Catalysis-dependent stabilization of Bre1 fine-tunes histone H2B ubiquitylation to regulate gene transcription

Monoubiquitylation of histone H2B on Lys123 (H2BK123ub1) plays a multifaceted role in diverse DNA-templated processes, yet the mechanistic details by which this modification is regulated are not fully elucidated. Here we show in yeast that H2BK123ub1 is regulated in part through the protein stability of the E3 ubiquitin ligase Bre1. We found that Bre1 stability is controlled by the Rtf1 subunit of the polymerase-associated factor (PAF) complex and through the ability of Bre1 to catalyze H2BK123ub1. Using a domain in Rtf1 that stabilizes Bre1, we show that inappropriate Bre1 levels lead to defects in gene regulation. Collectively, these data uncover a novel quality control mechanism used by the cell to maintain proper Bre1 and H2BK123ub1 levels, thereby ensuring proper control of gene expression

The Frequency of Barred Spiral Galaxies in the Near-IR

We have determined the fraction of barred galaxies in the H-band for a statistically well-defined sample of 186 spirals drawn from the Ohio State University Bright Spiral Galaxy survey. We find 56% of our sample to be strongly barred at H, while another 16% is weakly barred. Only 27% of our sample is unbarred in the near-infrared. The RC3 and the Carnegie Atlas of Galaxies both classify only about 30% of our sample as strongly barred. Thus strong bars are nearly twice as prevalent in the near-infrared as in the optical. The frequency of genuine optically hidden bars is significant, but lower than many claims in the literature: 40% of the galaxies in our sample that are classified as unbarred in the RC3 show evidence for a bar in the H-band, while for the Carnegie Atlas this fraction is 66%. Our data reveal no significant trend in bar fraction as a function of morphology in either the optical or H-band. Optical surveys of high redshift galaxies may be strongly biased against finding bars, as bars are increasingly difficult to detect at bluer rest wavelengths.Comment: LaTeX with AASTeX style file, 23 pages with 6 figures. Accepted for publication in The Astronomical Journal (Feb. 2000

On the origin of M81 group extended dust emission

Galactic cirrus emission at far-infrared wavelengths affects many extragalactic observations. Separating this emission from that associated with extragalactic objects is both important and difficult. In this paper we discuss a particular case, the M81 group, and the identification of diffuse structures prominent in the infrared, but also detected at optical wavelengths. The origin of these structures has previously been controversial, ranging from them being the result of a past interaction between M81 and M82 or due to more local Galactic emission. We show that over an order of a few arcmin scales, the far-infrared (Herschel 250 mu m) emission correlates spatially very well with a particular narrow-velocity (2-3 km s(-1)) component of the Galactic HI. We find no evidence that any of the far-infrared emission associated with these features actually originates in the M81 group. Thus we infer that the associated diffuse optical emission must be due to galactic light-back scattered off dust in our galaxy. Ultraviolet observations pick out young stellar associations around M81, but no detectable far-infrared emission. We consider in detail one of the Galactic cirrus features, finding that the far-infrared HI relation breaks down below arcmin scales and that at smaller scales there can be quite large dust-temperature variation

SPIRE imaging of M82: cool dust in the wind and tidal streams

M82 is a unique representative of a whole class of galaxies, starbursts with superwinds, in the Very Nearby Galaxy Survey with Herschel. In addition, its interaction with the M81 group has stripped a significant portion of its interstellar medium from its disk. SPIRE maps now afford better characterization of the far-infrared emission from cool dust outside the disk, and sketch a far more complete picture of its mass distribution and energetics than previously possible. They show emission coincident in projection with the starburst wind and in a large halo, much more extended than the PAH band emission seen with Spitzer. Some complex substructures coincide with the brightest PAH filaments, and others with tidal streams seen in atomic hydrogen. We subtract the far-infrared emission of the starburst and underlying disk from the maps, and derive spatially-resolved far-infrared colors for the wind and halo. We interpret the results in terms of dust mass, dust temperature, and global physical conditions. In particular, we examine variations in the dust physical properties as a function of distance from the center and the wind polar axis, and conclude that more than two thirds of the extraplanar dust has been removed by tidal interaction, and not entrained by the starburst wind.Comment: accepted in A&A Herschel special issu

Association of Taf14 with acetylated histone H3 directs gene transcription and the DNA damage response

The YEATS domain, found in a number of chromatin-associated proteins, has recently been shown to have the capacity to bind histone lysine acetylation. Here, we show that the YEATS domain of Taf14, a member of key transcriptional and chromatin-modifying complexes in yeast, is a selective reader of histone H3 Lys9 acetylation (H3K9ac). Structural analysis reveals that acetylated Lys9 is sandwiched in an aromatic cage formed by F62 and W81. Disruption of this binding in cells impairs gene transcription and the DNA damage response. Our findings establish a highly conserved acetyllysine reader function for the YEATS domain protein family and highlight the significance of this interaction for Taf14

SED fitting of nearby galaxies in the Herschel Reference Survey

We compute UV to radio continuum spectral energy distributions of 51 nearby galaxies recently observed with SPIRE onboard Herschel and present infrared colours (in the 25-500 μm spectral range). SPIRE data of normal galaxies are well reproduced with a modified black body (β=2) of temperature T≅q 20 K. In ellipticals hosting a radio galaxy, the far-infrared (FIR) emission is dominated by the synchrotron nuclear emission. The colour temperature of the cold dust is higher in quiescent E-S0a than in star-forming systems probably because of the different nature of their dust heating sources (evolved stellar populations, X-ray, fast electrons) and dust grain properties