Mechanism of NaCl transport-stimulated prostaglandin formation in MDCK cells

Recently we have found that stimulation of NaCl transport in high-resistance MDCK cells enhances their prostaglandin formation. In the present study, we investigated the mechanisms by which prostaglandin formation could be linked to the ion transport in these cells. We found that stimulation of transport caused a transient stimulation of prostaglandin formation lasting 5-10 min. The rise in prostaglandin formation was paralleled by a rise of free intracellular arachidonic acid. Analysis of membrane lipids revealed that the rise of free arachidonic acid was paralleled by a loss of arachidonic acid from polyphosphoinositides. We failed to obtain indications for the stimulation of calcium-dependent phospholipase A2. However, we did obtain evidence that the incorporation of arachidonic acid into phospholipids was diminished during stimulation of ion transport, indicating a decreased rate of reesterification. Despite the fact that there was no significant fall in total cellular ATP on stimulation of ion transport, we found a high and transient rise of lactate production of the cells on stimulation of the ion transport indicating an alteration of the ADP/ATP ratio. Moreover, prostaglandin formation and lactate formation were linearly correlated in this situation. When glucose utilization was inhibited by mannoheptulose, the rise in lactate formation was abolished, whereas that of PG formation was unaltered, indicating that lactate formation and prostaglandin formation were not causally linked on stimulation of ion transport. Our results suggest that an increase in the rate of sodium chloride transport by MDCK cells stimulates formation by an inhibition of reesterification of free arachidonic acid.(ABSTRACT TRUNCATED AT 250 WORDS

Energy Policy: A Test for Federalism

This Article will examine the bases of state and federal power, exploring areas of both potential and existing conflict within the energy field. Situations in which either the state or federal government appears to have exclusive authority also will be scrutinized. Possible answers to problems caused by the clashing of governmental interests will be suggested, with an eye toward aiding policymakers to reach agreements which may avert such conflicts. Finally, a prognosis of the future of federalism in regard to the energy issue will be offered

Plasma Magnetohydrodynamics and Energy Conversion

Contains reports on four research projects.National Science Foundation (Grant G-24073)United States Air Force, Aeronautical Systems Division, Aeronautical Accessories Laboratory, Wright-Patterson Air Force Base (Contract AF33(616)-7624

Plasmas and Controlled Nuclear Fusion

Contains reports on thirteen research projects split into two sections.National Science Foundation (Grant GK-57)National Science Foundation (Grant GK-614

Analysis of all available zodiacal light observations Final report

Interplanetary dust model ensemble derived for analysis of zodiacal light observation

Multidisciplinary Applications of Detached-Eddy Simulation to Separated Flows at High Reynolds Numbers

We focus on multidisciplinary applications of detached-eddy simulation (DES), principally flight mechanics and aeroelasticity. Specifically, the lateral instability (known as abrupt wing stall) of the preproduction F/A-18E is reproduced using DES, including the unsteady shock motion. The presence of low frequency pressure oscillations due to shock motion in the current simulations and the experiments motivated a full aircraft calculation, which showed low frequency high-magnitude rolling moments that could be a significant contributor to the abrupt wing stall phenomenon. DES is also applied to the F-18 high angle of attack research vehicle (HARV) at a moderate angle of attack to reproduce the vortex breakdown leading to vertical stabilizer buffet. Unsteady tail loads are compared to flight test data. This work lays the foundation for future deforming grid calculations to reproduce the aero-elastic tail buffet seen in flight test. Solution based grid adaption is used on unstructured grids in both cases to improve the resolution in the separated region. Previous DoD Challenge work has demonstrated the unique ability of the DES turbulence treatment to accurately and efficiently predict flows with massive separation at flight Reynolds numbers. DES calculations have been performed using the Cobalt code and on unstructured grids, an approach that can deal with complete configurations with very few compromises. A broad range of flows has been examined in previous Challenge work, including aircraft forebodies, airfoil sections, a missile afterbody, vortex breakdown on a delta wing, and the F-16 and F-15E at high angles-of-attack. All DES predictions exhibited a moderate to significant improvement over results obtained using traditional Reynolds-averaged models and often excellent agreement with experimental/flight-test data. DES combines the efficiency of a Reynolds-averaged turbulence model near the wall with the fidelity of Large-Eddy Simulation (LES) in separated regions. Since it uses Large-Eddy Simulation in the separated regions, it is capable of predicting the unsteady motions associated with separated flows. The development and demonstration of improved methods for the prediction of flight mechanics and aeroelasticity in this Challenge is expected to reduce the acquisition cost of future military aircraft

Northern Monterey Bay upwelling shadow front : observations of a coastally and surface-trapped buoyant plume

Author Posting. © American Geophysical Union, 2009. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 114 (2009): C12013, doi:10.1029/2009JC005623.During the upwelling season in central California, northwesterly winds along the coast produce a strong upwelling jet that originates at Point Año Nuevo and flows southward across the mouth of Monterey Bay. A convergent front with a mean temperature change of 3.77 ± 0.29°C develops between the warm interior waters and the cold offshore upwelling jet. To examine the forcing mechanisms driving the location and movement of the upwelling shadow front and its effects on biological communities in northern Monterey Bay, oceanographic conditions were monitored using cross-shelf mooring arrays, drifters, and hydrographic surveys along a 20 km stretch of coast extending northwestward from Santa Cruz, California, during the upwelling season of 2007 (May–September). The alongshore location of the upwelling shadow front at the northern edge of the bay was driven by: regional wind forcing, through an alongshore pressure gradient; buoyancy forces due to the temperature change across the front; and local wind forcing (the diurnal sea breeze). The upwelling shadow front behaved as a surface-trapped buoyant current, which is superimposed on a poleward barotropic current, moving up and down the coast up to several kilometers each day. We surmise that the front is advected poleward by a preexisting northward barotropic current of 0.10 m s−1 that arises due to an alongshore pressure gradient caused by focused upwelling at Point Año Nuevo. The frontal circulation (onshore surface currents) breaks the typical two-dimensional wind-driven, cross-shelf circulation (offshore surface currents) and introduces another way for water, and the material it contains (e.g., pollutants, larvae), to go across the shelf toward shore.Funded primarily by the Gordon and Betty Moore Foundation and the David and Lucile Packard Foundation

Plasmas and Controlled Nuclear Fusion

Contains research objectives and reports on four research projects.National Science Foundation (Grant GK-614)National Science Foundation (Grant GK-57