Tracing large-scale structures in circumstellar disks with ALMA

Planets are supposed to form in circumstellar disks. The gravitational potential of a planet perturbs the disk and leads to characteristic structures, i.e. spiral waves and gaps, in the disk's density profile. We perform a large-scale parameter study of the observability of these planet-induced structures in circumstellar disks with ALMA. On the basis of HD and MHD simulations, we calculated the disk temperature structure and (sub)mm images of these systems. These were used to derive simulated ALMA images. Because appropriate objects are frequent in Taurus, we focused on a distance of 140pc and a declination of 20{\deg}. The explored range of star-disk-planet configurations consists of 6 HD simulations (including magnetic fields and different planet masses), 9 disk sizes, 15 total disk masses, 6 different central stars, and two different grain size distributions. On almost all scales and in particular down to a scale of a few AU, ALMA is able to trace disk structures induced by planet-disk interaction or by the influence of magnetic fields on the wavelength range between 0.4 and 2.0mm. In most cases, the optimum angular resolution is limited by the sensitivity. However, within the range of typical masses of protoplanetary disks (0.1-0.001Msun) the disk mass has a minor impact on the observability. It is possible to resolve disks down to 2.67e-6Msun and trace gaps induced by a planet with M_p/M_s = 0.001 in disks with 2.67e-4Msun with a signal-to-noise ratio greater than three. The central star has a major impact on the observability of gaps, as well as the considered maximum grainsize of the dust in the disk. In general, it is more likely to trace planet-induced gaps in our MHD models, because gaps are wider in the presence of magnetic fields. We also find that zonal flows resulting from MRI create gap-like structures in the disk's re-emission radiation, which are observable with ALMA.Comment: 17 pages, 21 figure

Planet-induced disk structures: A comparison between (sub)mm and infrared radiation

Young giant planets, which are embedded in a circumstellar disk, will significantly perturb the disk density distribution. This effect can potentially be used as an indirect tracer for planets. We investigate the feasibility of observing planet-induced gaps in circumstellar disks in scattered light. We perform 3D hydrodynamical disk simulations combined with subsequent radiative transfer calculations in scattered light for different star, disk, and planet configurations. The results are compared to those of a corresponding study for the (sub)mm thermal re-emission. The feasibility of detecting planet-induced gaps in scattered light is mainly influenced by the optical depth of the disk and therefore by the disk size and mass. Planet-induced gaps are in general only detectable if the photosphere of the disks is sufficiently disturbed. Within the limitations given by the parameter space here considered, we find that gap detection is possible in the case of disks with masses below $\sim 10^{-4\dots-3} \, \rm M_\odot$. Compared to the disk mass that marks the lower Atacama Large (Sub)Millimeter Array (ALMA) detection limit for the thermal radiation re-emitted by the disk, it is possible to detect the same gap both in re-emission and scattered light only in a narrow range of disk masses around $\sim 10^{-4} \, \rm M_\odot$, corresponding to $16\%$ of cases considered in our study.Comment: 4 pages, 6 figure

Phosphocholine-Modified Lipooligosaccharides of Haemophilus influenzae Inhibit ATP-Induced IL-1beta Release by Pulmonary Epithelial Cells

Phosphocholine-modified bacterial cell wall components are virulence factors enabling immune evasion and permanent colonization of the mammalian host, by mechanisms that are poorly understood. Recently, we demonstrated that free phosphocholine (PC) and PC-modified lipooligosaccharides (PC-LOS) from Haemophilus influenzae, an opportunistic pathogen of the upper and lower airways, function as unconventional nicotinic agonists and efficiently inhibit the ATP-induced release of monocytic IL-1beta. We hypothesize that H. influenzae PC-LOS exert similar effects on pulmonary epithelial cells and on the complex lung tissue. The human lung carcinoma-derived epithelial cell lines A549 and Calu-3 were primed with lipopolysaccharide from Escherichia coli followed by stimulation with ATP in the presence or absence of PC or PC-LOS or LOS devoid of PC. The involvement of nicotinic acetylcholine receptors was tested using specific antagonists. We demonstrate that PC and PC-LOS efficiently inhibit ATP-mediated IL-1beta release by A549 and Calu-3 cells via nicotinic acetylcholine receptors containing subunits alpha7, alpha9, and/or alpha10. Primed precision-cut lung slices behaved similarly. We conclude that H. influenzae hijacked an endogenous anti-inflammatory cholinergic control mechanism of the lung to evade innate immune responses of the host. These findings may pave the way towards a host-centered antibiotic treatment of chronic airway infections with H. influenzae

Ultrafast optical ranging using microresonator soliton frequency combs

Light detection and ranging (LIDAR) is critical to many fields in science and industry. Over the last decade, optical frequency combs were shown to offer unique advantages in optical ranging, in particular when it comes to fast distance acquisition with high accuracy. However, current comb-based concepts are not suited for emerging high-volume applications such as drone navigation or autonomous driving. These applications critically rely on LIDAR systems that are not only accurate and fast, but also compact, robust, and amenable to cost-efficient mass-production. Here we show that integrated dissipative Kerr-soliton (DKS) comb sources provide a route to chip-scale LIDAR systems that combine sub-wavelength accuracy and unprecedented acquisition speed with the opportunity to exploit advanced photonic integration concepts for wafer-scale mass production. In our experiments, we use a pair of free-running DKS combs, each providing more than 100 carriers for massively parallel synthetic-wavelength interferometry. We demonstrate dual-comb distance measurements with record-low Allan deviations down to 12 nm at averaging times of 14 $\mu$s as well as ultrafast ranging at unprecedented measurement rates of up to 100 MHz. We prove the viability of our technique by sampling the naturally scattering surface of air-gun projectiles flying at 150 m/s (Mach 0.47). Combining integrated dual-comb LIDAR engines with chip-scale nanophotonic phased arrays, the approach could allow widespread use of compact ultrafast ranging systems in emerging mass applications.Comment: 9 pages, 3 figures, Supplementary information is attached in 'Ancillary files

Microresonator solitons for massively parallel coherent optical communications

Optical solitons are waveforms that preserve their shape while propagating, relying on a balance of dispersion and nonlinearity. Soliton-based data transmission schemes were investigated in the 1980s, promising to overcome the limitations imposed by dispersion of optical fibers. These approaches, however, were eventually abandoned in favor of wavelength-division multiplexing (WDM) schemes that are easier to implement and offer improved scalability to higher data rates. Here, we show that solitons may experience a comeback in optical communications, this time not as a competitor, but as a key element of massively parallel WDM. Instead of encoding data on the soliton itself, we exploit continuously circulating dissipative Kerr solitons (DKS) in a microresonator. DKS are generated in an integrated silicon nitride microresonator by four-photon interactions mediated by Kerr nonlinearity, leading to low-noise, spectrally smooth and broadband optical frequency combs. In our experiments, we use two interleaved soliton Kerr combs to transmit a data stream of more than 50Tbit/s on a total of 179 individual optical carriers that span the entire telecommunication C and L bands. Equally important, we demonstrate coherent detection of a WDM data stream by using a pair of microresonator Kerr soliton combs - one as a multi-wavelength light source at the transmitter, and another one as a corresponding local oscillator (LO) at the receiver. This approach exploits the scalability advantages of microresonator soliton comb sources for massively parallel optical communications both at the transmitter and receiver side. Taken together, the results prove the significant potential of these sources to replace arrays of continuous-wave lasers in high-speed communications.Comment: 10 pages, 3 figure

Molecular cloning, expression analysis and assignment of the porcine tumor necrosis factor superfamily member 10 gene (TNFSF10) to SSC13q34 -> q36 by fluorescence in situ hybridization and radiation hybrid mapping

We have cloned the complete coding region of the porcine TNFSF10 gene. The porcine TNFSF10 cDNA has an ORF of 870 nucleotides and shares 85 % identity with human TNFSF10, and 75% and 72% identity with rat and mouse Tnfsf10 coding sequences, respectively. The deduced porcine TNFSF10 protein consists of 289 amino acids with the calculated molecular mass of 33.5 kDa and a predicted pI of 8.15. The amino acid sequence similarities correspond to 86, 72 and 70% when compared with human, rat and mouse sequences, respectively. Nor-them blot analysis detected TNFSF10-specific transcripts (similar to 1.7 kb) in various organs of a 10-week-old pig, suggesting ubiquitous expression. Real-time RT-PCR studies of various organs from fetal (days 73 and 98) and postnatal stages (two weeks, eight months) demonstrated developmental and tissue-specific regulation of TNFSF10 mRNA abundance. The chromosomal location of the porcine TNFSF10 gene was determined by FISH of a specific BAC clone to metaphase chromosomes. This TNFSF10 BAC clone has been assigned to SSC13q34 -> q36. Additionally, the localization of the TNFSF10 gene was verified by RH mapping on the porcine IMpRH panel. Copyright (c) 2005S. KargerAG, Basel

Structural and doping effects in the half-metallic double perovskite A2A_2CrWO6_6

he structural, transport, magnetic and optical properties of the double perovskite $A_2$CrWO$_6$ with $A=\text{Sr, Ba, Ca}$ have been studied. By varying the alkaline earth ion on the $A$ site, the influence of steric effects on the Curie temperature $T_C$ and the saturation magnetization has been determined. A maximum $T_C=458$ K was found for Sr$_2$CrWO$_6$ having an almost undistorted perovskite structure with a tolerance factor $f\simeq 1$. For Ca$_2$CrWO$_6$ and Ba$_2$CrWO$_6$ structural changes result in a strong reduction of $T_C$. Our study strongly suggests that for the double perovskites in general an optimum $T_C$ is achieved only for $f \simeq 1$, that is, for an undistorted perovskite structure. Electron doping in Sr$_2$CrWO$_6$ by a partial substitution of Sr$^{2+}$ by La$^{3+}$ was found to reduce both $T_C$ and the saturation magnetization $M_s$. The reduction of $M_s$ could be attributed both to band structure effects and the Cr/W antisites induced by doping. Band structure calculations for Sr$_2$CrWO$_6$ predict an energy gap in the spin-up band, but a finite density of states for the spin-down band. The predictions of the band structure calculation are consistent with our optical measurements. Our experimental results support the presence of a kinetic energy driven mechanism in $A_2$CrWO$_6$, where ferromagnetism is stabilized by a hybridization of states of the nonmagnetic W-site positioned in between the high spin Cr-sites.Comment: 14 pages, 10 figure

Re-emergence of tularemia in Germany: Presence of <it>Francisella tularensis </it>in different rodent species in endemic areas

<p>Abstract</p> <p>Background</p> <p>Tularemia re-emerged in Germany starting in 2004 (with 39 human cases from 2004 to 2007) after over 40 years of only sporadic human infections. The reasons for this rise in case numbers are unknown as is the possible reservoir of the etiologic agent <it>Francisella (F.) tularensis</it>. No systematic study on the reservoir situation of <it>F. tularensis </it>has been published for Germany so far.</p> <p>Methods</p> <p>We investigated three areas six to ten months after the initial tularemia outbreaks for the presence of <it>F. tularensis </it>among small mammals, ticks/fleas and water. The investigations consisted of animal live-trapping, serologic testing, screening by real-time-PCR and cultivation.</p> <p>Results</p> <p>A total of 386 small mammals were trapped. <it>F. tularensis </it>was detected in five different rodent species with carrier rates of 2.04, 6.94 and 10.87% per trapping area. None of the ticks or fleas (n = 432) tested positive for <it>F. tularensis</it>. We were able to demonstrate <it>F. tularensis-</it>specific DNA in one of 28 water samples taken in one of the outbreak areas.</p> <p>Conclusion</p> <p>The findings of our study stress the need for long-term surveillance of natural foci in order to get a better understanding of the reasons for the temporal and spatial patterns of tularemia in Germany.</p

Control of human endometrial stromal cell motility by PDGF-BB, HB-EGF and trophoblast-secreted factors

Human implantation involves extensive tissue remodeling at the fetal-maternal interface. It is becoming increasingly evident that not only trophoblast, but also decidualizing endometrial stromal cells are inherently motile and invasive, and likely contribute to the highly dynamic processes at the implantation site. The present study was undertaken to further characterize the mechanisms involved in the regulation of endometrial stromal cell motility and to identify trophoblast-derived factors that modulate migration. Among local growth factors known to be present at the time of implantation, heparin-binding epidermal growth factor-like growth factor (HB-EGF) triggered chemotaxis (directed locomotion), whereas platelet-derived growth factor (PDGF)-BB elicited both chemotaxis and chemokinesis (non-directed locomotion) of endometrial stromal cells. Supernatants of the trophoblast cell line AC-1M88 and of first trimester villous explant cultures stimulated chemotaxis but not chemokinesis. Proteome profiling for cytokines and angiogenesis factors revealed neither PDGF-BB nor HB-EGF in conditioned media from trophoblast cells or villous explants, while placental growth factor, vascular endothelial growth factor and PDGF-AA were identified as prominent secretory products. Among these, only PDGF-AA triggered endometrial stromal cell chemotaxis. Neutralization of PDGF-AA in trophoblast conditioned media, however, did not diminish chemoattractant activity, suggesting the presence of additional trophoblast-derived chemotactic factors. Pathway inhibitor studies revealed ERK1/2, PI3 kinase/Akt and p38 signaling as relevant for chemotactic motility, whereas chemokinesis depended primarily on PI3 kinase/Akt activation. Both chemotaxis and chemokinesis were stimulated upon inhibition of Rho-associated, coiled-coil containing protein kinase. The chemotactic response to trophoblast secretions was not blunted by inhibition of isolated signaling cascades, indicating activation of overlapping pathways in trophoblast-endometrial communication. In conclusion, trophoblast signals attract endometrial stromal cells, while PDGF-BB and HB-EGF, although not identified as trophoblast-derived, are local growth factors that may serve to fine-tune directed and non-directed migration at the implantation site