System and market failures: the unavailability of magnesium sulphate for the treatment of eclampsia and pre-eclampsia in Mozambique and Zimbabwe.

Low cost and effective drugs, such as magnesium sulphate, need to be included in initiatives to improve access to essential medicines in Afric

Risk factors associated with contracting dysentery during shigella dysenteriae type 1 outbreak in Harare, 1993

A study to ascertain the cause, nature and management of an epidemic outbreak of diarrhea in Zimbabwe's urban areas in 1993.Towards the end of 1992, there were numerous reports of dysentery cases from Harare and most provinces of Zimbabwe. According to reports from the districts and the media, the disease was characterized by bouts of bloody diarrhea, vomiting and general body weakness. Of 4 915 patients with dysentery who presented themselves to the two municipal hospitals, 149 died, giving a case fatality rate of 3%.' Laboratory investigations confirmed that Shigella Dysenteriae type-1 as the predominant cause of the dysentery outbreak in Harare. A matched case control study, was carried out in the City of Harare from 26 November to 1 December, 1993 in order to document risk factors associated with contracting dysentery in this environment

The Cyclotron Fundamental Exposed in the High-Field Magnetic Variable V884 Her

High-quality phase-resolved optical spectropolarimetry is presented for the magnetic cataclysmic variable V884 Her. The overall circular polarization during active accretion states is low and only slightly variable in the range 5000-8000A. However, the polarization is highly structured with wavelength, showing very broad polarization humps, narrow features that are associated with weak absorption lines in the total spectral flux, and sharp reversals across each major emission line. The polarization reversals arise from Zeeman splitting in the funnel gas in a longitudinal magnetic field B~30kG. The set of narrow, polarized absorption features matches the Zeeman pattern of hydrogen for a nearly uniform magnetic field of B=150MG, indicating that the features are "halo" absorption lines formed in a relatively cool reversing layer above the shock. With this identification, the broad polarization humps centered near 7150A and below 4000A are assigned to cyclotron emission from the fundamental and first harmonic (n=2), respectively. V884 Her is only the second AM Her system known with a field exceeding 100MG, and the first case in which the cyclotron fundamental has been directly observed from a magnetic white dwarf.Comment: 14 pages, 6 figures, LaTeX, to appear in The Astrophysical Journal, Part

InGaAs MOMBE -- system drift and material quality

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29659/1/0000748.pd

The Three Dimensional Structure of EUV Accretion Regions in AM Herculis Stars: Modeling of EUV Photometric and Spectroscopic Observations

We have developed a model of the high-energy accretion region for magnetic cataclysmic variables and applied it to {\it Extreme Ultraviolet Explorer} observations of 10 AM Herculis type systems. The major features of the EUV light curves are well described by the model. The light curves exhibit a large variety of features such as eclipses of the accretion region by the secondary star and the accretion stream, and dips caused by material very close to the accretion region. While all the observed features of the light curves are highly dependent on viewing geometry, none of the light curves are consistent with a flat, circular accretion spot whose lightcurve would vary solely from projection effects. The accretion region immediately above the WD surface is a source of EUV radiation caused by either a vertical extent to the accretion spot, or Compton scattering off electrons in the accretion column, or, very likely, both. Our model yields spot sizes averaging 0.06 R$_{WD}$, or $f \sim 1 \times 10^{-3}$ the WD surface area, and average spot heights of 0.023 R$_{WD}$. Spectra extracted during broad dip phases are softer than spectra during the out-of-dip phases. This spectral ratio measurement leads to the conclusion that Compton scattering, some absorption by a warm absorber, geometric effects, an asymmetric temperature structure in the accretion region and an asymmetric density structure of the accretion columnare all important components needed to fully explain the data. Spectra extracted at phases where the accretion spot is hidden behind the limb of the WD, but with the accretion column immediately above the spot still visible, show no evidence of emission features characteristic of a hot plasma.Comment: 30 Pages, 11 Figure

The X-ray properties of the magnetic cataclysmic variable UUColumbae

Aims. XMM-Newton observations to determine for the first time the broad-band X-ray properties of the faint, high galactic latitude intermediate polar UUCol are presented. Methods. We performed X-ray timing analysis in different energy ranges of the EPIC cameras, which reveals the dominance of the 863 s white dwarf rotational period. The spin pulse is strongly energy dependent. Weak variabilities at the beat 935 s and at the 3.5 h orbital periods are also observed, but the orbital modulation is detected only below 0.5 keV. Simultaneous UV and optical photometry shows that the spin pulse is anti-phased with respect to the hard X-rays. Analysis of the EPIC and RGS spectra reveals the complexity of the X-ray emission, which is composed of a soft 50 eV black–body component and two optically thin emission components at 0.2 keV and 11 keV strongly absorbed by dense material with an equivalent hydrogen column density of 1023 cm−2 that partially (50%) covers the X-ray source. Results. The complex X-ray and UV/optical temporal behaviour indicates that accretion occurs predominantly (∼80%) via a disc with a partial contribution (∼20%) directly from the stream. The main accreting pole dominates at high energies whilst the secondary pole mainly contributes in the soft X-rays and at lower energies. The bolometric flux ratio of the soft-to-hard X-ray emissions is found to be consistent with the prediction of the standard accretion shock model. We find the white dwarf in UUCol accretes at a low rate and possesses a low magnetic moment. It is therefore unlikely that UUCol will evolve into a moderate field strength polar, so that the soft X-ray intermediate polars still remain an enigmatic small group of magnetic cataclysmic variables

Technical aspects of InGaAs MOMBE -- shutter action, system drift, and material quality

Lattice matched InxGa1 - x As films were deposited on InP substrates using metalorganic molecular beam epitaxy (MOMBE) with trimethylindium (TMIn), triethylgallium (TEGa) and a solid arsenic source. The effect of growth temperature and molecular beam composition on growth rate and crystal composition was investigated. A long term drift of the molecular beam composition and an increasing difference between temperature readings of the thermocouple and the pyrometer were observed. The corrected data show a linear dependence of crystal composition on molecular beam composition. Shutter action on TMIn and TEGa was investigated. The results show the adverse effect of solely using the shutters to control the metalorganic molecular beam, leading to inferior material quality and rough surface morphology. Material grown in the optimized process consistently showed electron mobilities of [mu]300 [approximate] 9000 cm2/V[middle dot]s and [mu]77 [approximate] 35,000 cm2/[middle dot]s at 1.2 x 1015 cm-3 n-type background concentration.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29421/1/0000497.pd

Induction of fibroblast senescence generates a non-fibrogenic myofibroblast phenotype that differentially impacts on cancer prognosis

Cancer-associated fibroblasts (CAF) remain a poorly characterized, heterogeneous cell population. Here we characterized two previously described tumor-promoting CAF sub-types, smooth muscle actin (SMA)-positive myofibroblasts and senescent fibroblasts, identifying a novel link between the two

Quantitative non-canonical amino acid tagging based proteomics identifies distinct patterns of protein synthesis rapidly induced by hypertrophic agents in cardiomyocytes, revealing new aspects of metabolic remodeling

Cardiomyocytes undergo growth and remodeling in response to specific pathological or physiological conditions. Pathological myocardial growth is a risk factor for cardiac failure to which faster protein synthesis is a major driving element. We aimed to quantify the rapid effects of different pro-hypertrophic stimuli on the synthesis of specific proteins in ARVC and to determine whether such effects are due to alterations on mRNA abundance or the translation of specific mRNAs. Cardiomyocytes have very low rates of protein synthesis, posing a challenging problem in terms of studying changes in the synthesis of specific proteins, which also applies to other non-dividing primary cells. To address this, an optimized QuaNCAT LC/MS method was used to selectively quantify newly synthesized proteins in such cells. The study showed both classical (phenylephrine; PE) and more recent (insulin) pathological cardiac hypertrophic agents increased the synthesis of proteins involved in glycolysis, the Krebs cycle / beta-oxidation, and sarcomeric components. However, insulin increased synthesis of many metabolic enzymes to a greater extent than PE. Using a novel validation method, we confirmed that synthesis of selected candidates is indeed up-regulated by PE and insulin. Synthesis of all proteins studied was upregulated by signaling through mTORC1 without changes in their mRNA levels, showing the key importance of translational control in the rapid effects of hypertrophic stimuli. Expression of PKM2 was upregulated in rat hearts following TAC. This isoform possesses specific regulatory properties that may be involved in metabolic remodeling and as a novel candidate biomarker. Levels of translation factor eEF1 also increased during TAC, likely contributing to faster cell mass accumulation. Interestingly, PKM2 and eEF1 were not up-regulated in pregnancy or exercise induced CH, suggesting them as pathological CH specific markers. The study methods may be of utility to the examination of protein synthesis in primary cells

Regulation of Translesion Synthesis DNA Polymerase η by Monoubiquitination

DNA polymerase eta is a Y family polymerase involved in translesion synthesis (TLS). Its action is initiated by simultaneous interaction between the PIP box in pol eta and PCNA and between the UBZ in pol eta and monoubiquitin attached to PCNA. Whereas monoubiquitination of PCNA is required for its interaction with pol eta during TLS, we now show that monoubiquitination of pol eta inhibits this interaction, preventing its functions in undamaged cells. Identification of monoubiquitination sites within pol eta nuclear localization signal (NLS) led to the discovery that pol eta NLS directly contacts PCNA, forming an extended pol eta-PCNA interaction surface. We name this the PCNA-interacting region (PIR) and show that its monoubiquitination is downregulated by various DNA-damaging agents. We propose that this mechanism ensures optimal availability of nonubiquitinated, TLS-competent pol eta after DNA damage. Our work shows how monoubiquitination can either positively or negatively regulate the assembly of a protein complex, depending on which substrates are targeted by ubiquitin