Cosmology with next generation radio telescopes

Philosophiae Doctor - PhDThe next generation of radio telescopes will revolutionize cosmology by providing large three-dimensional surveys of the universe. This work presents forecasts using the technique 21cm intensity mapping (IM) combined with results from the cosmic microwave background, or mock data of galaxy surveys. First, we discuss prospects of constraining curvature independently of the dark energy (DE) model, finding that the radio instrument HIRAX will reach percent-level accuracy even when an arbitrary DE equation of state is assumed. This is followed by a study of the potential of the multi-tracer technique to surpass the cosmic variance limit, a crucial method to probe primordial non-Gaussianity and large scale general relativistic e↵ects. Using full sky simulations for the Square Kilometre Array phase 1 (SKA 1 MID) and the Large Synoptic Survey Telescope (LSST), including foregrounds, we demonstrate that the cosmic variance contaminated scenario can be beaten even in the noise free case. Finally, we derive the signal to noise ratio for the cosmic magnification signal from foreground HI intensity maps combined with background galaxy count maps. Instruments like SKA1 MID and HIRAX are highly complementary and well suited for this measurement. Thanks to the powerful design of the planned radio instruments, all results confirm their potential and promise an exciting future for cosmology

Simulating the Large-Scale Structure of HI Intensity Maps

Intensity mapping of neutral hydrogen (HI) is a promising observational probe of cosmology and large-scale structure. We present wide field simulations of HI intensity maps based on N-body simulations of a $2.6\, {\rm Gpc / h}$ box with $2048^3$ particles (particle mass $1.6 \times 10^{11}\, {\rm M_\odot / h}$). Using a conditional mass function to populate the simulated dark matter density field with halos below the mass resolution of the simulation ($10^{8}\, {\rm M_\odot / h} < M_{\rm halo} < 10^{13}\, {\rm M_\odot / h}$), we assign HI to those halos according to a phenomenological halo to HI mass relation. The simulations span a redshift range of 0.35 < z < 0.9 in redshift bins of width $\Delta z \approx 0.05$ and cover a quarter of the sky at an angular resolution of about 7'. We use the simulated intensity maps to study the impact of non-linear effects and redshift space distortions on the angular clustering of HI. Focusing on the autocorrelations of the maps, we apply and compare several estimators for the angular power spectrum and its covariance. We verify that these estimators agree with analytic predictions on large scales and study the validity of approximations based on Gaussian random fields, particularly in the context of the covariance. We discuss how our results and the simulated maps can be useful for planning and interpreting future HI intensity mapping surveys.Comment: 35 pages, 19 Figures. Accepted for publication in JCA

MuSK induces in vivo acetylcholine receptor clusters in a ligand-independent manner

Muscle-specific receptor tyrosine kinase (MuSK) is required for the formation of the neuromuscular junction. Using direct gene transfer into single fibers, MuSK was expressed extrasynaptically in innervated rat muscle in vivo to identify its contribution to synapse formation. Spontaneous MuSK kinase activity leads, in the absence of its putative ligand neural agrin, to the appearance of ε-subunit–specific transcripts, the formation of acetylcholine receptor clusters, and acetylcholinesterase aggregates. Expression of kinase-inactive MuSK did not result in the formation of acetylcholine receptor (AChR) clusters, whereas a mutant MuSK lacking the ectodomain did induce AChR clusters. The contribution of endogenous MuSK was excluded by using genetically altered mice, where the kinase domain of the MuSK gene was flanked by loxP sequences and could be deleted upon expression of Cre recombinase. This allowed the conditional inactivation of endogenous MuSK in single muscle fibers and prevented the induction of ectopic AChR clusters. Thus, the kinase activity of MuSK initiates signals that are sufficient to induce the formation of AChR clusters. This process does not require additional determinants located in the ectodomain

Prospects for cosmic magnification measurements using HI intensity mapping

We investigate the prospects of measuring the cosmic magnification effect by cross-correlating neutral hydrogen intensity mapping (H I IM) maps with background optical galaxies. We forecast the signal-to-noise ratio for H i IM data from SKA1-MID and HIRAX, combined with LSST photometric galaxy samples. We find that, thanks to their different resolutions, SKA1-MID and HIRAX are highly complementary in such an analysis. We predict that SKA1-MID can achieve a detection with a signal-to-noise ratio of ∼15 on a multipole range of ℓ ≲ 200, while HIRAX can reach a signal-to-noise ratio of ∼50 on 200 < ℓ < 2000. We conclude that measurements of the cosmic magnification signal will be possible on a wide redshift range with foreground H I intensity maps up to z ≲ 2, while optimal results are obtained when 0.6 ≲ z ≲ 1.3

Simulated multi-tracer analyses with HI intensity mapping

We use full sky simulations, including the effects of foreground contamination and removal, to explore multi-tracer synergies between a SKA-like 21cm intensity mapping survey and a LSST-like photometric galaxy redshift survey. In particular we study ratios of auto and cross-correlations between the two tracers as estimators of the ratio of their biases, a quantity that should benefit considerably from the cosmic variance cancellation of the multi-tracer approach. We show how well we should be able to measure the bias ratio on very large scales (down to $\ell \sim 3$), which is crucial to measure primordial non-Gaussianity and general relativistic effects on large scale structure. We find that, in the absence of foregrounds but with realistic noise levels of such surveys, the multi-tracer estimators are able to improve on the sensitivity of a cosmic-variance contaminated measurement by a factor of $2-4$. When foregrounds are included, estimators using the 21cm auto-correlation become biased. However, we show that cross-correlation estimators are immune to this and do not incur in any significant penalty in terms of sensitivity from discarding the auto-correlation data. However, the loss of long-wavelength radial modes caused by foreground removal in combination with the low redshift resolution of photometric surveys, reduces the sensitivity of the multi-tracer estimator, albeit still better than the cosmic variance contaminated scenario even in the noise free case. Finally we explore different alternative avenues to avoid this problem.Comment: 14 pages, 11 figures, 1 tabl

HR outsourcing:The impact on HR's strategic role and remaining in-house HR function

Past research on HR outsourcing (HRO) has offered conflicting views about its impact on HR's strategic position. This study highlights the processes by which decisions to outsource HR are made, followed by the processes implemented post such decisions and their effect on the HR function. Using a case study approach and semi-structured interviews (N = 35) within a German subsidiary of a US MNC, we provide a framework of HR processes seeking to achieve standardization in terms of harmonization of HR activities across the subsidiary. The findings reveal that the effects of outsourcing on in-house HR showed a decrease in flexibility of the HR function, a slowdown in processing time of transactional HR as well as a decrease in satisfaction and work intensification for HR managers. Further, it remains questionable as to whether the function of HR was able to enhance its strategic position through outsourcing

Motor Endplate—Anatomical, Functional, and Molecular Concepts in the Historical Perspective

By mediating voluntary muscle movement, vertebrate neuromuscular junctions (NMJ) play an extraordinarily important role in physiology. While the significance of the nerve-muscle connectivity was already conceived almost 2000 years back, the precise cell and molecular biology of the NMJ have been revealed in a series of fascinating research activities that started around 180 years ago and that continues. In all this time, NMJ research has led to fundamentally new concepts of cell biology, and has triggered groundbreaking advancements in technologies. This review tries to sketch major lines of thought and concepts on NMJ in their historical perspective, in particular with respect to anatomy, function, and molecular components. Furthermore, along these lines, it emphasizes the mutual benefit between science and technology, where one drives the other. Finally, we speculate on potential major future directions for studies on NMJ in these fields

Accumulation of muscle ankyrin repeat protein transcript reveals local activation of primary myotube endcompartments during muscle morphogenesis

The characteristic shapes and positions of each individual body muscle are established during the process of muscle morphogenesis in response to patterning information from the surrounding mesenchyme. Throughout muscle morphogenesis, primary myotubes are arranged in small parallel bundles, each myotube spanning the forming muscles from end to end. This unique arrangement potentially assigns a crucial role to primary myotube end regions for muscle morphogenesis. We have cloned muscle ankyrin repeat protein (MARP) as a gene induced in adult rat skeletal muscle by denervation. MARP is the rodent homologue of human C-193 (Chu, W., D.K. Burns, R.A. Swerick, and D.H. Presky. 1995. J. Biol. Chem. 270:10236-10245) and is identical to rat cardiac ankyrin repeat protein. (Zou, Y., S. Evans, J. Chen, H.-C. Kuo, R.P. Harvey, and K.R. Chien. 1997. Development. 124:793-804). In denervated muscle fibers, MARP transcript accumulated in a unique perisynaptic pattern. MARP was also expressed in large blood vessels and in cardiac muscle, where it was further induced by cardiac hypertrophy. During embryonic development, MARP was expressed in forming skeletal muscle. In situ hybridization analysis in mouse embryos revealed that MARP transcript exclusively accumulates at the end regions of primary myotubes during muscle morphogenesis. This closely coincided with the expression of thrombospondin-4 in adjacent prospective tendon mesenchyme, suggesting that these two compartments may constitute a functional unit involved in muscle morphogenesis. Transfection experiments established that MARP protein accumulates in the nucleus and that the levels of both MARP mRNA and protein are controlled by rapid degradation mechanisms characteristic of regulatory early response genes. The results establish the existence of novel regulatory muscle fiber subcompartments associated with muscle morphogenesis and denervation and suggest that MARP may be a crucial nuclear cofactor in local signaling pathways from prospective tendon mesenchyme to forming muscle and from activated muscle interstitial cells to denervated muscle fibers

Induction of adult-type nicotinic acetylcholine receptor gene expression in noninnervated regenerating muscle

Expression of adult-type nicotinic acetylcholine receptors at the neuromuscular junction is thought to result from selective induction of their genes in endplate-associated nuclei due to local neurotrophic control. However, denervation studies indicate that endplate-specific expression can be maintained in the absence of the nerve. We investigated the role played by the basal lamina in this expression by assaying for the adult-type-specific [var epsilon] RNA in noninnervated regenerating muscle. We found that this RNA is locally expressed beneath the old endplates after 10 days of regeneration. At earlier times [var epsilon] RNA is also found in areas other than the endplate region. These results indicate that in adult muscle the basal lamina contains all the components necessary to direct nicotinic acetylcholine receptor gene expression to the endplate.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29114/1/0000153.pd