498 research outputs found
Analysis of surface moisture variations within large field sites
A statistical analysis was made on ground soils to define the general relationship and ranges of values of the field moisture relative to both the variance and coefficient of variation for a given test site and depth increment. The results of the variability study show that: (1) moisture variations within any given large field area are inherent and can either be controlled nor reduced; (2) neither a single value of the standard deviation nor coefficient of variation uniquely define the variability over the complete range of mean field moisture contents examined; and (3) using an upper bound standard deviation parameter clearly defines the maximum range of anticipated moisture variability. 87 percent of all large field moisture content standard deviations were less than 3 percent while about 96 percent of all the computed values had an upper bound of sigma=4 percent for these intensively sampled fields. The limit of accuracy curves of mean soil moisture measurements for large field sites relative to the required number of samples were determined
Vier Arzneimittelinteraktionsdatenbanken im Vergleich:eine Untersuchung im Kontext komplexer pädiatrisch-onkologischer Arzneimittelverordnungen
Pädiatrisch-onkologische Patienten repräsentieren ein Risikokollektiv für Arzneimittelinteraktionen. Können Arzneimittelinteraktionsdatenbanken als hilfreiche und effiziente Entscheidungsunterstützungssysteme, sowohl zur Detektion von, als auch zur Informationsgewinnung über potentielle Interaktionen dienen? Es erfolgte ein Vergleich der Arzneimittelinteraktionsdatenbanken Drug-Reax®, Drug Interaction Facts®, ifap index®KLINIK und Lexi-Interact® anhand einer Arzneimittelliste, und in einer Anwendungsphase anhand von Medikationsprofilen von 50 Patienten der Station für pädiatrische Hämatologie und Onkologie. Durch die fehlende Konkordanz zwischen den Datenbanken, das Fehlen einer standardisierten Bewertung des Schweregrades und der klinischen Relevanz potentieller Interaktionen, sowie einer Betrachtung patientenspezifischer Risikofaktoren, erscheinen die untersuchten Interaktionsdatenbanken für den klinischen Gebrauch momentan noch wenig praktikabel und können zurzeit eher als Screeningtool dienen
PCV22 OUTCOMES ANALYSIS OF PROFILACTICAL HEALTH CARE PROGRAMS, BASED ON “HEART VESSEL PROPHYLACTIC PROGRAM” FOR 40 AND 50 YEAR OLD RESIDENTS OF WROCLAW CITY
Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice
Background:
A short-term high-fat diet impairs mitochondrial function and the ability of skeletal muscle to respond to growth stimuli, but it is unknown whether such a diet alters the ability to respond to atrophy signals. The purpose of this study was to determine whether rapid weigh gain induced by a high-fat (HF) diet accelerates denervation-induced muscle atrophy.
Methods:
Adult, male mice (C57BL/6) were fed a control or HF (60Â % calories as fat) diet for 3Â weeks (3wHF). Sciatic nerve was sectioned unilaterally for the final 5 or 14Â days of the diet. Soleus and extensor digitorum longus (EDL) muscles were removed and incubated in vitro to determine rates of protein degradation and subsequently homogenized for determination of protein levels of LC3, ubiquitination, myosin heavy chain (MHC) distribution, and mitochondrial subunits.
Results:
When mice were fed the 3wHF diet, whole-body fat mass more than doubled, but basal (innervated) muscle weights, rates of protein degradation, LC3 content, mitochondrial protein content, and myosin isoform distribution were not significantly different than with the control diet in either soleus or EDL. However in the 14Â day denervated soleus, the 3wHF diet significantly augmented loss of mass, proteolysis rate, amount of the autophagosome marker LC3 II, and the amount of overall ubiquitination as compared to the control fed mice. On the contrary, the 3wHF diet had no significant effect in the EDL on amount of mass loss, proteolysis rate, LC3 levels, or ubiquitination. Fourteen days denervation also induced a loss of mitochondrial proteins in the soleus but not the EDL, regardless of the diet.
Conclusions:
Taken together, a short-term, high-fat diet augments denervation muscle atrophy by induction of protein degradation in the mitochondria-rich soleus but not in the glycolytic EDL. These findings suggest that the denervation-induced loss of mitochondria and HF diet-induced impairment of mitochondrial function may combine to promote skeletal muscle atrophy
Evaluating the HYPE model for estimating groundwater recharge in a groundwater dominated catchment in Poland
Hydrological models can be useful tools simulating climate and land use changes and their impact on nutrients outflows from a catchment area. One of them is the HYPE (HYdrological Predictions for the Environment) water quality model applicable to different spatial scales. Groundwater recharge via infiltrating precipitation is a significant water budget component. The rate of groundwater recharge in the HYPE model is estimated from the water balance in soils. The Kocinka river catchment is one of the test areas in the BONUS-Soils2Sea project where HYPE model modelling was carried out. A hydrograph, among others, is one of the modelling results and, based on it, the recharge rate of groundwater was determined. This value was compared with groundwater recharge rates estimated by the infiltration method used for the Groundwater Vulnerability Map of Poland
Constitutively Active CaMKKα Stimulates Skeletal Muscle Glucose Uptake in Insulin-Resistant Mice In Vivo
In insulin-sensitive skeletal muscle, the expression of constitutively active Ca(2+)/calmodulin-dependent protein kinase kinase α (caCaMKKα) stimulates glucose uptake independent of insulin signaling (i.e., Akt and Akt-dependent TBC1D1/TBC1D4 phosphorylation). Our objectives were to determine whether caCaMKKα could stimulate glucose uptake additively with insulin in insulin-sensitive muscle, in the basal state in insulin-resistant muscle, and if so, to determine whether the effects were associated with altered TBC1D1/TBC1D4 phosphorylation. Mice were fed a control or high-fat diet (60% kcal) for 12 weeks to induce insulin resistance. Muscles were transfected with empty vector or caCaMKKα plasmids using in vivo electroporation. After 2 weeks, caCaMKKα protein was robustly expressed. In insulin-sensitive muscle, caCaMKKα increased basal in vivo [(3)H]-2-deoxyglucose uptake approximately twofold, insulin increased glucose uptake approximately twofold, and caCaMKKα plus insulin increased glucose uptake approximately fourfold. caCaMKKα did not increase basal TBC1D1 (Ser(237), Thr(590), Ser(660), pan-Thr/Ser) or TBC1D4 (Ser(588), Thr(642), pan-Thr/Ser) phosphorylation. In insulin-resistant muscle, caCaMKKα increased basal glucose uptake approximately twofold, and attenuated high-fat diet–induced basal TBC1D1 (Thr(590), pan-Thr/Ser) and TBC1D4 (Ser(588), Thr(642), pan-Thr/Ser) phosphorylation. In cell-free assays, CaMKKα increased TBC1D1 (Thr(590), pan-Thr/Ser) and TBC1D4 (Ser(588), pan-Thr/Ser) phosphorylation. Collectively, these results demonstrate that caCaMKKα stimulates glucose uptake additively with insulin, and in insulin-resistant muscle, and alters the phosphorylation of TBC1D1/TBC1D4
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