16 research outputs found
Futureproofing [18F]Fludeoxyglucose manufacture at an Academic Medical Center
Abstract
Background
We recently upgraded our [18F]fludeoxyglucose (FDG) production capabilities with the goal of futureproofing our FDG clinical supply, expanding the number of batches of FDG we can manufacture each day, and improving patient throughput in our nuclear medicine clinic. In this paper we report upgrade of the synthesis modules to the GE FASTLab 2 platform (Phase 1) and cyclotron updates (Phase 2) from both practical and regulatory perspectives. We summarize our experience manufacturing FDG on the FASTLab 2 module with a high-yielding self-shielded niobium (Nb) fluorine-18 target.
Results
Following installation of Nb targets for production of fluorine-18, a 55 μA beam for 22 min generated 1330 ± 153 mCi of [18F]fluoride. Using these cyclotron beam parameters in combination with the FASTLab 2, activity yields (AY) of FDG were 957 ± 102 mCi at EOS, corresponding to 72% non-corrected AY (n = 235). Our workflow, inventory management and regulatory compliance have been greatly simplified following the synthesis module and cyclotron upgrades, and patient wait times for FDG PET have been cut in half at our nuclear medicine clinic.
Conclusions
The combination of FASTlab 2 and self-shielded Nb fluorine-18 targets have improved our yield of FDG, and enabled reliable and repeatable manufacture of the radiotracer for clinical use.https://deepblue.lib.umich.edu/bitstream/2027.42/145727/1/41181_2018_Article_48.pd
Automated Radiosynthesis of [<sup>18</sup>F]FluoFAPI and Its Dosimetry and Single Acute Dose Toxicological Evaluation
Background: Cancer-associated fibroblasts have become a new target for therapy. Fibroblasts present within malignancies express the fibroblast activation protein (FAP). Inhibitors to FAP (FAPI) are small molecules recently developed as a theranostic agents for imaging and radiotherapy. All currently used FAPI rely on a linker–chelator complex attached to the ‘inhibitor’. We describe a new automated method of the direct attachment of the radioisotope to the inhibitor, resulting in a >50% MW reduction with the hope of an improved tumor-to-background ratio and tumor uptake. Methods: [18F]FluroFAPI was developed from a Sn precursor. This allowed for subsequent automated radioflourination. We obtained the biodistribution of [18F]FluroFAPI in rats, performed estimated human radiation dosimetry, and performed a 100× expected single dose toxicology analysis for eventual first-in-human experiments. Results: The synthesis of the Sn precursor for FluorFAPI and the automated synthesis of [18F]FluroFAPI was demonstrated. [18F]FluroFAPI had favorable estimated human radiation dosimetry, and demonstrated no adverse effects when injected at a dose of 100× that planned for [18F]FluroFAPI. Conclusions: With the successful development of an automated synthesis of [18F]FluroFAPI, first-in-human testing can be planned with the hope of an improved tumor-to-background performance compared to other FAPI agents
3AcFNP-59 for Positron Emission Tomography Imaging of Cholesterol Trafficking and Utilization
Cholesteryl ester analogues of [18F]FNP-59
have the
ability to provide information on cholesterol trafficking and utilization
at earlier time points than those of [18F]FNP-59 or [131I]NP-59. It is well-known that free cholesterol and cholesteryl
esters have differing distribution profiles and that they can be interconverted
enzymatically. Substitution of the ester influences the rate of cholesterol
ester hydrolysis and the subsequent mixing of cholesterol esters with
the lipid pool in the body. This can be utilized by preparing esters
that are more readily taken up by lipoprotein, are quickly hydrolyzed
and mixed with the endogenous lipid pool and delivered to tissues
of interest more quickly than free cholesterol analogues that require
esterification for lipoprotein association. The acetyl ester of FNP-59
demonstrated the preferred uptake properties and response to adrenal
cortical manipulation, indicating its ability to image hormone production.
Finally, dosimetry studies were conducted in preparation for the clinical
translation of [18F]3AcFNP-59