Validity of oral mucosal transudate specimens for HIV testing using enzyme-linked immunosorbent assay in children in Chimanimani district, Zimbabwe

Objective. To assess the validity of oral mucosal transudate (OMT) specimens for HIV testing in children using enzyme-linked immunosorbent assay (ELISA). Methods. A cross-sectional descriptive study was conducted as part of a community-based behavioural and HIV sero-status survey of adults and children in the Chimanimani district of Zimbabwe. Dried blood spot (DBS) and OMT samples were collected from children aged between 2 and 14 years, inclusive. Both samples were tested for HIV using the Vironostika Uniform II plus O kits. The main study outcomes were the sensitivity and specificity of OMT samples, with DBS as the gold-standard specimen. Results. Paired DBS and OMT specimens were available from 1 274 (94.4%) of the 1 350 children enrolled. Using the DBS, HIV prevalence was 3.2%. Overall sensitivity of OMT was 48.8% (95% confidence interval (CI) 33.3 - 64.5), and specificity was 98.5% (95% CI 97.7 - 99.1). Conclusion. The overall sensitivity of OMT specimens for HIV testing in children using ELISA was low. Stratifying the analysis by sector showed that OMT samples are good specimens for HIV testing. It is important to note that factors such as the low HIV prevalence in our study population, quality of the OMT, diet and oral hygiene could have influenced the results

The human resource implications of improving financial risk protection for mothers and newborns in Zimbabwe

Peer reviewedPublisher PD

Carbapenem resistance expressed by Gram-negative bacilli isolated from a cohort of Libyan patients

Background and objectives: Carbapenem-resistant Enterobacteriaceae (CRE) and other Gram-negative bacteria are among the most common pathogens responsible for both community and hospital acquired infection. The global spread of cephalosporinases in Enterobacteriaceae has led to the increased use of carbapenems resulting in the emergence and rapid spread of CRE. This has become an alarming public health concern, yet the condition in Libya remains unclear. The aim of this study was to obtain a better understanding of CRE strains prevalent in Libyan patients by investigating their phenotypic characteristics and antibiograms. Methods: Gram-negative bacterial species were collected from Misrata Central Hospital, Misrata Cancer Centre and Privet Pathology Laboratories. Clinical samples and swabs were obtained from hospitalised and non-hospitalised patients and from mechanical ventilation and suction machines. Patients who had received antibiotic therapy for at least three days prior to the study were excluded. The identification and characterization of the isolated species were achieved using the growth characteristics on MacConkey and blood agar, spot tests and API 20E or API 20NE biochemical testing systems. Screening for carbapenem resistance was performed using the disk diffusion method with carbapenem 10 μg and cephalosporin 30 μg disks and minimum inhibitory concentrations (MIC) determined using the Sensititre Gram-negative Xtra plate format (GNX2F). All strains demonstrating resistance or reduced susceptibility to one of the four carbapenems were subjected to carbapenememase activity detection using the RAPIDEC CARBA NP test, Modified Hodge test and carbapenem inactivation methods. Results: A total of one hundred and forty isolates representing fourteen bacterial species were isolated from 140 non-duplicated specimens. Clinical specimens included urine samples (96/140, 68.57%), sputum (15/140, 10.71%), surgical wound swabs (18/140, 12.85%), foot swabs from diabetes mellitus (DM) patients (6/140, 4.29%), ear swabs (3/140, 2.14%) and wound swabs (2/140, 1.43%). Thirty-four (24.29%) isolates demonstrated resistance to at least one of the four carbapenems with Klebsiella pneumoniae representing 73.53% (25 isolates) of all carbapenem resistant species, followed by 8.82% for Pseudomonas aeruginosa (3 isolates), 5.88% for both Proteus mirabilis (2 isolates) and Escherichia coli (2 isolates) and 2.94% for both Citrobacter koseri (1 isolate) and Rahnella aquatilis (1 isolate). The other isolates were either susceptible or cephalosporinase producers. Conclusion: This study has revealed the high rate of carbapenem resistance amongst Libyan patients and emphasizes the crucial need for accurate screening, identification and susceptibility testing to prevent further spread of nosocomial and community acquired resistance. This may be achieved through the establishment of antibiotic stewardship programmes along with firm infection control practices.National Research Foundation of South Africa; Libyan GovernmentWeb of Scienc

The acute phase response to parturition:a cross-sectional study in Zimbabwe

Parturition triggers an acute phase response, but its magnitude, duration and predictors are not well described. We determined serum a1-antichymotrypsin (ACT) and C-reactive protein (CRP) among 216women attending postpartum services in south-eastern Zimbabwe. Serum CRP peaked during the first week and serum ACT around 9 days postpartum. Serum ACT, but not serum CRP, was lower amongHIV infected women. Multiparity was a negative, and preterm delivery and caesarean section were positive predictors of both serum ACT and CRP. There is a need for a better understanding of the acute phase response to parturition (Afr J Reprod Health 2009; 13[2]:61-68)

Réponse de la phase aiguë a la parturition: une étude transversale au Zimbabwe

Parturition triggers an acute phase response, but its magnitude, duration and predictors are not well described. We determined serum α1-antichymotrypsin (ACT) and C-reactive protein (CRP) among 216 women attending postpartum services in south-eastern Zimbabwe. Serum CRP peaked during the first week and serum ACT around 9 days postpartum. Serum ACT, but not serum CRP, was lower among HIV infected women. Multiparity was a negative, and preterm delivery and caesarean section were positive predictors of both serum ACT and CRP. There is a need for a better understanding of the acute phase response to parturition (Afr J Reprod Health 2009; 13[2]:61-68).La parturition déclenché une réponse de la phase aiguë, mais son ampleur, sa durée et ses indices ne Sont pas bien décrits. Nous avons détermine la sérique alpha-antichymotrypsin (AAT) et la protéine C-réactive (PCR) chez 216 femmes qui fréquentaient les services du post-partum au sud-est du Zimbabwe. La sérique PCR a atteint le maximum au cours de la première semaine et la sérique AAT après un post-partum d’à peu près neuf jours. La sérique AAT (non pas la sérique PCR) était moins élevée chez les femmes séropositives. La multiparité était un indice négatif, tandis que l’accouchement avant terme et l’opération césarienne étaient des indices positifs de la sérique AAT et PCR. Il faut une meilleure compréhension de la réponse de la phase aiguë à la parturition (Afr J Reprod Health 2009; 13[2]:61-68)

Flexibility of deployment: challenges and policy options for retaining health workers during crisis in Zimbabwe

Zimbabwe experienced a socio-economic crisis from 1997 to 2008 which heavily impacted all sectors. In this context, human resource managers were confronted with the challenge of health worker shortage in rural areas and, at the same time, had to operate under a highly centralised, government-centred system which defined health worker deployment policies. This study examines the implementation of deployment policies in Zimbabwe before, during and after the crisis in order to analyse how the official policy environment evolved over time, present the actual practices used by managers to cope with the crisis and draw lessons. ‘Deployment’ here was considered to include all the human resource management functions for getting staff into posts and managing subsequent movements: recruitment, bonding, transfer and secondment. The study contributes to address the existing paucity of evidence on flexibility on implementation of policies in crisis/conflict setting