Ultimate opportunists - The emergent Enterocytozoon group Microsporidia

This is the final version. Available on open access from Public Library of Science via the DOI in this recordDefraBritish Council Newton FundUniversity of Exete

Operating theatre photography for orthopaedics and aesthetic surgery.

The aim of this paper is to examine the author's personal experience and practice in operating theatre photography. The ways of working are personal to the author but hopefully will help others in undertaking this type of work

Membrane receptors of mouse leukocytes. II. Sequential expression of membrane receptors and phagocytic capacity during leukocyte differentiation

Analysis of four mature cell markers on mouse bone marrow leukocytes grown in vitro, demonstrated a distinct sequence of marker appearance during the terminal phases of granulocytic cell differentiation. A similar pattern of marker expression was also suggested by analysis of mature neutrophils and macrophages isolated from normal tissues. Among cultured neutrophils, receptors for the Fc portion of IgG (FcR) were first expressed on myelocytes and metamyelocytes, and then subsequently on more mature cells. Morphologically mature colony neutrophils (polymorphs) from agar cultures contained only FcR and complement receptor type two (CR(2)) (C3d receptor), and lacked both complement receptor type one (CR(1)) (C3b receptor) and the capacity to ingest latex, bacteria, or iron particles. Neutrophils from 2 and 3 wk liquid media cultures of marrow cells differed from agar grown neutrophils in that they had phagocytic capacity (particle ingestion) [Pi] in addition to FcR and CR(2). Furthermore, in the 4th and 5th wk of these continuous liquid cultures, CR(1) was also expressed, completing the surface marker profile of normal blood neutrophils. Based on these studies, the following order of appearance of these four markers on cells from the myelocytic series was proposed: FcR {arrow} FcR CR(2) {arrow} FcR CR(2) Pi {arrow} FcR CR(2) Pi CR(1). Differential studies of tissue leukocytes containing these same markers revealed that a heterogeneity existed among morphologically mature neutrophils. Even though 95 percent of blood polymorphs contained all four markers, the same was true of only half of spleen polymorphs and only 20 percent of bone marrow polymorphs. Cells of the monocyte-macrophage series were studies in parallel with neutrophils. Cultured marrow monocytes acquired the four mature cell markers so rapidly that the order of receptor appearance could not be determined. However, it was found that CR2 was lost during the terminal phase of monocyte maturation into activated macrophages

Outer zone electrons

Spatial and temporal behavior of high energy trapped electrons in outer zone of magnetospher

Liquid-phase synthesis of 2′-methyl-RNA on a homostar support through organic-solvent nanofiltration

Due to the discovery of RNAi, oligonucleotides (oligos) have re-emerged as a major pharmaceutical target that may soon be required in ton quantities. However, it is questionable whether solid-phase oligo synthesis (SPOS) methods can provide a scalable synthesis. Liquid-phase oligo synthesis (LPOS) is intrinsically scalable and amenable to standard industrial batch synthesis techniques. However, most reported LPOS strategies rely upon at least one precipitation per chain extension cycle to separate the growing oligonucleotide from reaction debris. Precipitation can be difficult to develop and control on an industrial scale and, because many precipitations would be required to prepare a therapeutic oligonucleotide, we contend that this approach is not viable for large-scale industrial preparation. We are developing an LPOS synthetic strategy for 2′-methyl RNA phosphorothioate that is more amenable to standard batch production techniques, using organic solvent nanofiltration (OSN) as the critical scalable separation technology. We report the first LPOS-OSN preparation of a 2′-Me RNA phosphorothioate 9-mer, using commercial phosphoramidite monomers, and monitoring all reactions by HPLC, (31)P NMR spectroscopy and MS

A nested PCR assay to avoid false positive detection of the microsporidian enterocytozoon hepatopenaei (EHP) in environmental samples in shrimp farms

PublishedJournal Article© 2016 Jaroenlak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP) is an important disease of cultivated shrimp. Heavy infections may lead to retarded growth and unprofitable harvests. Existing PCR detection methods target the EHP small subunit ribosomal RNA (SSU rRNA) gene (SSU-PCR). However, we discovered that they can give false positive test results due to cross reactivity of the SSU-PCR primers with DNA from closely related microsporidia that infect other aquatic organisms. This is problematic for investigating and monitoring EHP infection pathways. To overcome this problem, a sensitive and specific nested PCR method was developed for detection of the spore wall protein (SWP) gene of EHP (SWP-PCR). The new SWP-PCR method did not produce false positive results from closely related microsporidia. The first PCR step of the SWP-PCR method was 100 times (104 plasmid copies per reaction vial) more sensitive than that of the existing SSU-PCR method (106 copies) but sensitivity was equal for both in the nested step (10 copies). Since the hepatopancreas of cultivated shrimp is not currently known to be infected with microsporidia other than EHP, the SSU-PCR methods are still valid for analyzing hepatopancreatic samples despite the lower sensitivity than the SWP-PCR method. However, due to its greater specificity and sensitivity, we recommend that the SWP-PCR method be used to screen for EHP in feces, feed and environmental samples for potential EHP carriers.OI acknowledges support from Agricultural Research Development Agency under project CRP5905020530 and Mahidol University. KS received funding from National Research Council Thailand, Division of Plan Administration and Research Budget/2557-79. PJ is supported by the Science Achievement Scholarship of Thailand (SAST). GDS acknowledges support of DG SANCO of the European Commission, and the UK Department of Environment, Food and Rural Affairs under project FB002. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

New Paradigms to Help Solve the Global Aquaculture Disease Crisis.

Published onlineJournal ArticleThis is the final version of the article. Available from Public Library of Science via the DOI in this record.n/aThe authors (GDS, KS) acknowledge funding administered by the British Council under the Newton Fund Researcher Links Programme, for a UK-Thailand bilateral workshop entitled "Scientific, technological and social solutions for sustainable aquaculture in Thailand: a key player in global aquatic food supply," Bangkok, March 2016. Further funding support is acknowledged from the European Commission (EC) and the UK Department for Environment, Food and Rural Affairs (Defra) under contracts C6928 and FB002 (to GDS and DB); from the Royal Society under a University Research Fellowship (to BAPW); and to the Agricultural Research Development Agency (ARDA) and National Research Council of Thailand (NRCT) (to KS, TWF, and OI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Seasonality of warm water intrusions onto the continental shelf near the Totten Glacier

Warm Modified Circumpolar Deep Water (MCDW) from the Southern Ocean drives rapid basal melt of the Totten Ice Shelf on the Sabrina Coast (East Antarctica), affecting the mass balance of the grounded Totten Glacier. Recent observations show that MCDW intrudes onto the continental shelf through a depression at the shelf break. Here we investigate such intrusions by combining (1) new oceanographic and bathymetric observations collected for two consecutive years by profiling floats in the depression south of the shelf break, (2) oceanographic measurements collected by conductivity‐temperature‐depth‐instrumented seals on continental slope, and (3) an ocean model. The depression provides a pathway for persistent inflow of warm (0‐1°C) MCDW to the inner shelf. In austral autumn and early winter MCDW intrusions were up to 0.5°C warmer and were are ~75 m thicker than in spring and summer. The seasonality of the flow on the continental slope explains the seasonality of the intrusions. The MCDW layer on the continental slope is warmer and thicker to the east of the depression than to the west. In autumn and early winter a strong, top‐to‐bottom westward current (Antarctic Slope Current) transports the warmer and thicker MCDW layer along the slope and is diverted poleward at the eastern entrance of the depression. A bottom‐intensified eastward current (Antarctic Slope Undercurrent) develops in other months, allowing cooler and thinner intrusions to enter the depression from the west. Our study illustrates how circulation on the Antarctic slope regulates the ocean heat delivery to the continental shelf and ultimately to the ice shelves

Distribution of water masses and meltwater on the continental shelf near the Totten and Moscow University ice shelves

Warm waters flood the continental shelf of the Amundsen and Bellingshausen seas in West Antarctica, driving rapid basal melt of ice shelves. In contrast, waters on the continental shelf in East Antarctica are cooler and ice shelves experience relatively low rates of basal melt. An exception is provided by the Totten and Moscow University ice shelves on the Sabrina Coast, where satellite-derived basal melt rates are comparable to West Antarctica. Recent oceanographic observations have revealed that relatively warm (∼−0.4°C) modified Circumpolar Deep Water (mCDW) enters the cavity beneath the Totten Ice Shelf through a 1100 m deep trough, delivering sufficient heat to drive rapid basal melt. Here we use observations from a recent summer survey to show that mCDW is widespread on the continental shelf of the Sabrina Coast, forming a warm (up to 0.3°C) and saline (34.5–34.6) bottom layer overlaid by cold (∼freezing point) and fresh (salinity ∼34.3) Winter Water. Dense Shelf Water is not observed. A 1000 deep m trough allows water at −1.3°C to reach the Moscow University ice-shelf cavity to drive basal melt. Freshening by addition of glacial meltwater is widespread on the southern shelf at depths above 300–400 m, with maximum meltwater concentrations up to 4–5 ml L−1 observed in outflows from the ice-shelf cavities. Our observations indicate that the ocean properties on the Sabrina Coast more resemble those found on the continental shelf of the Amundsen and Bellingshausen seas than those typical of East Antarctica