The Effect of Maternal Diet on Fetal Development

Using mice as a model system, this study aimed to compare the effect of a high fat diet (HFD), high sugar diet (HSD) and a control diet (CD) on fetal growth and development at the time of birth (post-natal day 0 or PND 0), post-natal day 7 (PND 7) and post-natal day 14 (PND 14). Published literature has documented the effects of a negative in-utero environment on fetal development, but currently there is limited research regarding the continued effects on development after birth. Mouse pups were harvested and then imaged in 3-D using a high-resolution micro-computed tomography (micro-CT) system. Total bone volume, average bone mineral density, and limb lengths were quantified individually for each pup using the micro-CT images. At PND 0, limb lengths, bone mineral densities, and total bone volumes were significantly decreased in HFD and HSD pups compared to CD samples. By PND 7, limb length measurements for the HFD caught up to the CD samples. Pups on the HSD showed evidence of accelerated “catch-up” growth as well. By PND 14 there were no differences between the HFD, HSD and the CD. This indicates that while maternal diet during gestation profoundly influences pre-natal growth, pups exposed to intra-uterine HFD or HSD exhibit “catch-up” growth post-natally. While outcome measures for the HFD and HSD pups normalize to CD specimens prior to weaning, this rapid catch-up growth has been shown to lead to negative health effects later in life

Encouraging teacher change within the realities of school-based agricultural education: lessons from teachers’ initial use of socioscientific issues-based instruction

Calls for increased interdisciplinary education have led to the development of numerous teaching techniques designed to help teachers provide meaningful experiences for their students. However, methods of guiding teachers in the successful adoption of innovative teaching approaches are not firmly set. This qualitative study sought to better understand how school-based agricultural education teachers decide to adopt or discontinue a teaching innovation when introduced through ready-made lesson plans, which is currently a common practice of teaching method integration in school-based agricultural education (SBAE). Constant comparative analysis unveiled themes within the reactions to the teaching method’s use, as well as how teacher actions to those reactions led to their ultimate adoption or discontinuance of the teaching method

Effects of Energy and Protein Levels and Antibiotics on Growing Turkeys

Much attention has been given recently to the use and value of high energy rations for poultry, particularly for broilers and to a limited extent for laying hens and young turkeys. Although the general fundamentals of the findings may appear applicable in formulation of diets for growing turkeys beyond the age of 8 weeks, little direct evidence has been available to justify such application. Actually, there is some evidence to indicate that the higher energy diets may not necessarily promote a more rapid rate of gain for the older birds. The current study considers the possible effects of protein and antibiotics on the problem

Growth and body composition in pku children— a three-year prospective study comparing the effects of l-amino acid to glycomacropeptide protein substitutes

Protein quality and quantity are important factors in determining lean body (muscle) mass (LBM). In phenylketonuria (PKU), protein substitutes provide most of the nitrogen, either as amino acids (AA) or glycomacropeptide with supplementary amino acids (CGMP-AA). Body composition and growth are important indicators of long-term health. In a 3-year prospective study comparing the impact of AA and CGMP-AA on body composition and growth in PKU, 48 children were recruited. N = 19 (median age 11.1 years, range 5–15 years) took AA only, n = 16 (median age 7.3 years, range 5–15 years) took a combination of CGMP-AA and AA, (CGMP50) and 13 children (median age 9.2 years, range 5–16 years) took CGMP-AA only (CGMP100). A dual energy X-ray absorptiometry (DXA) scan at enrolment and 36 months measured LBM, % body fat (%BF) and fat mass (FM). Height was measured at enrolment, 12, 24 and 36 months. No correlation or statistically significant differences (after adjusting for age, gender, puberty and phenylalanine blood concentrations) were found between the three groups for LBM, %BF, FM and height. The change in height z scores, (AA 0, CGMP50 +0.4 and CGMP100 +0.7) showed a trend that children in the CGMP100 group were taller, had improved LBM with decreased FM and % BF but this was not statistically significant. There appeared to be no advantage of CGMP-AA compared to AA on body composition after 3-years of follow-up. Although statistically significant differences were not reached, a trend towards improved body composition was observed with CGMP-AA when it provided the entire protein substitute requirement.publishersversionpublishe

Direct numerical simulation of a tip-leakage flow in a planar duct with a longitudinal slit

A planar duct flow configuration with a cross-flow injected from a longitudinal slit close to the upper wall of the duct is studied by using a direct numerical simulation approach to explore the underlying flow mechanism in relation to the tip-leakage vortex (TLV), which is one of the most important flow phenomena in turbomachinery. Major characteristics of TLV in a rotor of turbomachinery are identified in the current flow model. The analysis of mean and instantaneous flow fields reveals that the interaction between the main (axial) flow and jet (cross) flow is the primary source of the generation of the TLV. The evolution of the TLV is then investigated, and a vortex breakup phenomenon is identified. The evolution of TLV can be divided into three phases, i.e. the formation phase, the break-up phase, and the diffusion phase. Mean streamlines and turbulence kinetic energy (TKE) budgets are analysed, showing that the high TKE central spot in the formation phase is due to the interaction between highly swirling vortex filaments and mean velocity gradient. In the outer part of the TLV, the TKE is mainly produced in the shear-layer and transported towards the centre by the turbulence transport

Internet tool to support self-assessment and self-swabbing of sore throat: development and feasibility study

Background: Sore throat is a common problem and a common reason for the overuse of antibiotics. A web-based tool that helps people assess their sore throat, through the use of clinical prediction rules, taking throat swabs or saliva samples, and taking throat photographs, has the potential to improve self-management and help identify those who are the most and least likely to benefit from antibiotics. Objective: We aimed to develop a web-based tool to help patients and parents or carers self-assess sore throat symptoms and take throat photographs, swabs, and saliva samples for diagnostic testing. We then explored the acceptability and feasibility of using the tool in adults and children with sore throats. Methods: We used the Person-Based Approach to develop a web-based tool and then recruited adults and children with sore throats who participated in this study by attending general practices or through social media advertising. Participants self-assessed the presence of FeverPAIN and Centor score criteria and attempted to photograph their throat and take throat swabs and saliva tests. Study processes were observed via video call, and participants were interviewed about their views on using the web-based tool. Self-assessed throat inflammation and pus were compared to clinician evaluation of patients’ throat photographs. Results: A total of 45 participants (33 adults and 12 children) were recruited. Of these, 35 (78%) and 32 (71%) participants completed all scoring elements for FeverPAIN and Centor scores, respectively, and most (30/45, 67%) of them reported finding self-assessment relatively easy. No valid response was provided for swollen lymph nodes, throat inflammation, and pus on the throat by 11 (24%), 9 (20%), and 13 (29%) participants respectively. A total of 18 (40%) participants provided a throat photograph of adequate quality for clinical assessment. Patient assessment of inflammation had a sensitivity of 100% (3/3) and specificity of 47% (7/15) compared with the clinician-assessed photographs. For pus on the throat, the sensitivity was 100% (3/3) and the specificity was 71% (10/14). A total of 89% (40/45), 93% (42/45), 89% (40/45), and 80% (30/45) of participants provided analyzable bacterial swabs, viral swabs, saliva sponges, and saliva drool samples, respectively. Participants were generally happy and confident in providing samples, with saliva samples rated as slightly more acceptable than swab samples. Conclusions: Most adult and parent participants were able to use a web-based intervention to assess the clinical features of throat infections and generate scores using clinical prediction rules. However, some had difficulties assessing clinical signs, such as lymph nodes, throat pus, and inflammation, and scores were assessed as sensitive but not specific. Many participants had problems taking photographs of adequate quality, but most were able to take throat swabs and saliva samples

Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell development and endocrine hormone expression

AbstractNkx2.2 and Arx are essential pancreatic transcription factors. Nkx2.2 is necessary for the appropriate specification of the islet alpha, beta, PP and epsilon cell lineages, whereas Arx is required to form the correct ratio of alpha, beta, delta and PP cells. To begin to understand the cooperative functions of Nkx2.2 and Arx in the development of endocrine cell lineages, we generated progenitor cell-specific deletions of Arx on the Nkx2.2 null background. The analysis of these mutants demonstrates that expansion of the ghrelin cell population in the Nkx2.2 null pancreas is not dependent on Arx; however, Arx is necessary for the upregulation of ghrelin mRNA levels in Nkx2.2 mutant epsilon cells. Alternatively, in the absence of Arx, delta cell numbers are increased and Nkx2.2 becomes essential for the repression of somatostatin gene expression. Interestingly, the dysregulation of ghrelin and somatostatin expression in the Nkx2.2/Arx compound mutant (Nkx2.2null;ArxΔpanc) results in the appearance of ghrelin+/somatostatin+ co-expressing cells. These compound mutants also revealed a genetic interaction between Nkx2.2 and Arx in the regulation of the PP cell lineage; the PP cell population is reduced when Nkx2.2 is deleted but is restored back to wildtype numbers in the Nkx2.2null;ArxΔpanc mutant. Moreover, conditional deletion of Arx in specific pancreatic cell populations established that the functions of Arx are necessary in the Neurog3+ endocrine progenitors. Together, these experiments identify novel genetic interactions between Nkx2.2 and Arx within the endocrine progenitor cells that ensure the correct specification and regulation of endocrine hormone-producing cells