Chemical composition and mixing in giant HII regions: NGC3603, 30Doradus, and N66

We investigate the chemical abundances of NGC3603 in the Milky Way, of 30Doradus in the Large Magellanic Cloud, and of N66 in the Small Magellanic Cloud. Mid-infrared observations with the Infrared Spectrograph onboard the Spitzer Space Telescope allow us to probe the properties of distinct physical regions within each object: the central ionizing cluster, the surrounding ionized gas, photodissociation regions, and buried stellar clusters. We detect [SIII], [SIV], [ArIII], [NeII], [NeIII], [FeII], and [FeIII] lines and derive the ionic abundances. Based on the ionic abundance ratio (NeIII/H)/(SIII/H), we find that the gas observed in the MIR is characterized by a higher degree of ionization than the gas observed in the optical spectra. We compute the elemental abundances of Ne, S, Ar, and Fe. We find that the alpha-elements Ne, S, and Ar scale with each other. Our determinations agree well with the abundances derived from the optical. The Ne/S ratio is higher than the solar value in the three giant HII regions and points toward a moderate depletion of sulfur on dust grains. We find that the neon and sulfur abundances display a remarkably small dispersion (0.11dex in 15 positions in 30Doradus), suggesting a relatively homogeneous ISM, even though small-scale mixing cannot be ruled out.Comment: Accepted for submission to ApJ. The present version replaces the submitted one. Changes: new title, new figure, the text was modified in the discussio

Partial Dynamical Symmetry and Mixed Dynamics

Partial dynamical symmetry describes a situation in which some eigenstates have a symmetry which the quantum Hamiltonian does not share. This property is shown to have a classical analogue in which some tori in phase space are associated with a symmetry which the classical Hamiltonian does not share. A local analysis in the vicinity of these special tori reveals a neighbourhood of phase space foliated by tori. This clarifies the suppression of classical chaos associated with partial dynamical symmetry. The results are used to divide the states of a mixed system into ``chaotic'' and ``regular'' classes.Comment: 10 pages, Revtex, 3 figures, Phys. Rev. Lett. in pres

Oral tolerance to cancer can be abrogated by T regulatory cell inhibition

Oral administration of tumour cells induces an immune hypo-responsiveness known as oral tolerance. We have previously shown that oral tolerance to a cancer is tumour antigen specific, non-cross-reactive and confers a tumour growth advantage. We investigated the utilisation of regulatory T cell (Treg) depletion on oral tolerance to a cancer and its ability to control tumour growth. Balb/C mice were gavage fed homogenised tumour tissue – JBS fibrosarcoma (to induce oral tolerance to a cancer), or PBS as control. Growth of subcutaneous JBS tumours were measured; splenic tissue excised and flow cytometry used to quantify and compare systemic Tregs and T effector (Teff) cell populations. Prior to and/or following tumour feeding, mice were intraperitoneally administered anti-CD25, to inactivate systemic Tregs, or given isotype antibody as a control. Mice which were orally tolerised prior to subcutaneous tumour induction, displayed significantly higher systemic Treg levels (14% vs 6%) and faster tumour growth rates than controls (p<0.05). Complete regression of tumours were only seen after Treg inactivation and occurred in all groups - this was not inhibited by tumour feeding. The cure rates for Treg inactivation were 60% during tolerisation, 75% during tumour growth and 100% during inactivation for both tolerisation and tumour growth. Depletion of Tregs gave rise to an increased number of Teff cells. Treg depletion post-tolerisation and post-tumour induction led to the complete regression of all tumours on tumour bearing mice. Oral administration of tumour tissue, confers a tumour growth advantage and is accompanied by an increase in systemic Treg levels. The administration of anti-CD25 Ab decreased Treg numbers and caused an increase in Teffs. Most notably Treg cell inhibition overcame established oral tolerance with consequent tumor regression, especially relevant to foregut cancers where oral tolerance is likely to be induced by the shedding of tumour tissue into the gut

Uncovering the Outflow Driven by the Brown Dwarf LS-RCr A1: H-alpha as a Tracer of Outflow Activity in Brown Dwarfs

It is now apparent that classical T Tauri-like outflows commonly accompany the formation of young brown dwarfs. To date two optical outflows have been discovered and results presented in this paper increase this number to three. Using spectro-astrometry the origin of the LS-RCrA 1 forbidden emission lines in a blue-shifted outflow is confirmed. The non-detection of the red-shifted component of the outflow in forbidden lines, along with evidence for some separation between low and high velocity outflow components, do not support the hypothesis that LS-RCrA 1 has an edge-on accretion disk. The key result of this analysis is the discovery of an outflow component to the H-alpha line. The H-alpha line profile has blue and red-shifted features in the wings which spectro-astrometry reveals to also originate in the outflow. The discovery that H-alpha emission in BDs can have a significant contribution from an outflow suggests the use of H-alpha line widths as a proxy of mass accretion in BDs is not clear-cut. This method assumes that any contribution to the H-alpha line flux from a possible outflow is negligible. Finally the fact that the H-alpha line traces both lobes of the outflow while only the blue-shifted lobe is seen in forbidden emission points to the presence of a dust hole in the accretion disk of LS-RCrA 1. This is commonly seen in CTTSs and is assumed to signal the onset of planet formation.Comment: 13 pages, 2 figure

A versatile, automated and high-throughput drug screening platform for zebrafish embryos

Zebrafish provide a unique opportunity for drug screening in living animals, with the fast developing, transparent embryos allowing for relatively high-throughput, microscopy-based screens. However, the limited availability of rapid, flexible imaging and analysis platforms has limited the use of zebrafish in drug screens. We have developed an easy-to-use, customisable automated screening procedure suitable for high-throughput phenotype-based screens of live zebrafish. We utilised the WiScan® Hermes High Content Imaging System to rapidly acquire brightfield and fluorescent images of embryos, and the WiSoft® Athena Zebrafish Application for analysis, which harnesses an Artificial Intelligence-driven algorithm to automatically detect fish in brightfield images, identify anatomical structures, partition the animal into regions, and exclusively select the desired side-oriented fish. Our initial validation combined structural analysis with fluorescence images to enumerate GFP-tagged haematopoietic stem and progenitor cells in the tails of embryos, which correlated with manual counts. We further validated this system to assess the effects of genetic mutations and x-ray irradiation in high content using a wide range of assays. Further, we performed simultaneous analysis of multiple cell types using dual fluorophores in high throughput. In summary, we demonstrate a broadly applicable and rapidly customisable platform for high-content screening in zebrafish

Recovery of the X-Ray Transient QX Nor (=X1608-52) in Outburst and Quiescence

We present optical and near-IR observations of QX Nor, the counterpart to the recurrent soft X-ray transient X1608-52, after its reappearance following the X-ray outburst in February 1996. The object has been seen only once before, during an X-ray outburst in 1977. Data from 3-5 months after the outburst show the counterpart at a mean magnitude of R=20.2 and variable on timescales of days. A comparison with identical observations in 1995 implies that the object has brightened by at least 1.8 mag in R following the X-ray outburst. We also detected QX Nor in the IR in both quiescence and outburst. A faint source is visible in the J but not the R band in May 1995. These first observations in the quiescent state yield magnitudes and colors consistent with optical emission from a low mass companion in the binary system, as is true in other soft X-ray transients.Comment: 10 pages including 4 figures and 2 tables; Uses AASTeX 4.0; Accepted for publication in The Astrophysical Journal, Volume 485, August 20, 199

Model Flames in the Boussinesq Limit: The Effects of Feedback

We have studied the fully nonlinear behavior of pre-mixed flames in a gravitationally stratified medium, subject to the Boussinesq approximation. Key results include the establishment of criterion for when such flames propagate as simple planar flames; elucidation of scaling laws for the effective flame speed; and a study of the stability properties of these flames. The simplicity of some of our scalings results suggests that analytical work may further advance our understandings of buoyant flames.Comment: 11 pages, 14 figures, RevTex, gzipped tar fil

Periodic Orbits and Spectral Statistics of Pseudointegrable Billiards

We demonstrate for a generic pseudointegrable billiard that the number of periodic orbit families with length less than $l$ increases as $\pi b_0l^2/\langle a(l) \rangle$, where $b_0$ is a constant and $\langle a(l) \rangle$ is the average area occupied by these families. We also find that $\langle a(l) \rangle$ increases with $l$ before saturating. Finally, we show that periodic orbits provide a good estimate of spectral correlations in the corresponding quantum spectrum and thus conclude that diffraction effects are not as significant in such studies.Comment: 13 pages in RevTex including 5 figure

Energy Level Statistics of the U(5) and O(6) Symmetries in the Interacting Boson Model

We study the energy level statistics of the states in U(5) and O(6) dynamical symmetries of the interacting boson model and the high spin states with backbending in U(5) symmetry. In the calculations, the degeneracy resulting from the additional quantum number is eliminated manually. The calculated results indicate that the finite boson number $N$ effect is prominent. When $N$ has a value close to a realistic one, increasing the interaction strength of subgroup O(5) makes the statistics vary from Poisson-type to GOE-type and further recover to Poisson-type. However, in the case of $N \to \infty$, they all tend to be Poisson-type. The fluctuation property of the energy levels with backbending in high spin states in U(5) symmetry involves a signal of shape phase transition between spherical vibration and axial rotation.Comment: 38 pages, 13 figure