Aversive Stimuli Drive Drug Seeking in a State of Low Dopamine Tone

Background Stressors negatively impact emotional state and drive drug seeking, in part, by modulating the activity of the mesolimbic dopamine system. Unfortunately, the rapid regulation of dopamine signaling by the aversive stimuli that cause drug seeking is not well characterized. In a series of experiments, we scrutinized the subsecond regulation of dopamine signaling by the aversive stimulus, quinine, and tested its ability to cause cocaine seeking. Additionally, we examined the midbrain regulation of both dopamine signaling and cocaine seeking by the stress-sensitive peptide, corticotropin releasing factor (CRF). Methods Combining fast-scan cyclic voltammetry with behavioral pharmacology, we examined the effect of intraoral quinine administration on nucleus accumbens dopamine signaling and hedonic expression in 21 male Sprague-Dawley rats. We tested the role of CRF in modulating aversion-induced changes in dopamine concentration and cocaine seeking by bilaterally infusing the CRF antagonist, CP-376395, into the ventral tegmental area (VTA). Results We found that quinine rapidly reduced dopamine signaling on two distinct time scales. We determined that CRF acted in the VTA to mediate this reduction on only one of these time scales. Further, we found that the reduction of dopamine tone and quinine-induced cocaine seeking were eliminated by blocking the actions of CRF in the VTA during the experience of the aversive stimulus. Conclusions These data demonstrate that stress-induced drug seeking can occur in a terminal environment of low dopamine tone that is dependent on a CRF-induced decrease in midbrain dopamine activity

Characteristics of Wound-Associated Wood of Yellow-Poplar (Liriodendron Tulipifera L.)

Selected anatomical characteristics and specific gravity of yellow-poplar wood formed after wounding and adjacent to the wound were compared to similar characteristics of yellow-poplar wood formed before and after wounding and away from the wound. The wood formed immediately after wounding was similar anatomically to the barrier zones described for other species. Vessel volume, vessel diameter, percentage of vessel multiples, and vessel element length were significantly lower in wound-associated wood, while ray volume, ray density, and specific gravity were significantly greater. Such changes in the vessel system would result in a decrease in conductivity in the wounded area, while the increase in parenchyma would increase the potential for manufacture of fungitoxic compounds. With increasing radial distance from the wound area, the anatomical features of the wound-associated wood gradually approached those of normal wood, although by four years after wounding, the wood still had not returned to normal. The specific gravity stayed significantly greater

Cloud fraction, layer, and direction of movement results from sky cameras during the FIRE IFO, Coffeyville, Kansas, experiment for the period Nov. 12 through Dec. 9, 1991

Tables and figures are presented which show local site observations of cloud fractions, the number of cloud layers, direction of movement, and precipitation data collected during the FIRE (First ISCCP Regional Experiment) Phase 2 Cirrus Intensive Field Observations (IFO) conducted in Coffeyville, Kansas during November and December, 1991. Selected data are also presented at the times of the TIROS Operational Vertical Sounder (TOVS) satellite overpass. Several major scientific projects have used surface-based observations of clouds to compare directly with those being observed from satellites. Characterizing the physical properties of clouds is extremely useful in obtaining a more accurate analysis of the effect of clouds and their movements on weather and climate. It is the purpose of this paper to report data collected during the FIRE Phase 2 IFO experiment and to provide a brief history of such a surface-based system and the technical information required for recording local cloud parameters

The Exceptionally Luminous Type Ia Supernova 2007If

SN 2007if was the third over-luminous Type Ia supernova (SN Ia) detected after 2003fg and 2006gz. We present the photometric and spectroscopic observations of the SN and its host by ROTSE-III, HET, and Keck. From the H a line identified in the host spectra, we determine a redshift of 0.0736. At this distance, the SN reached an absolute magnitude of -20.4, brighter than any other SNe Ia ever observed. If the source of luminosity is radioactive decay, a large amount of radioactive nickel (similar to 1.5 M(circle dot)) is required to power the peak luminosity, more than can be produced realistically in a Chandrasekhar mass progenitor. Low expansion velocity, similar to that of 2003fg, is also measured around the maximum light. The observations may suggest that SN 2007if was from a massive white dwarf progenitor, plausibly exploding with mass well beyond 1.4 M(circle dot). Alternatively, we investigate circumstellar interaction that may contribute to the excess luminosity.NASA NNX-08AN25G, NNX-08AV63GNSF AST-0707769, PHY-0801007Australian Research CouncilUniversity of New South WalesUniversity of TexasUniversity of MichiganAstronom

Physiological Disorders in Closed, Controlled Environment Crops

This slide presentation reviews some of the physiological disorders that affect crops grown in closed controlled environments. A physiological disorder is understood to be a problem resulting from the influence of environmental and horticultural factors on plan development other than a problem caused by a pathogen or some other abiotic cause. The topics that are addressed are: (1) Calcium-Related Disorders (2) Oedema (Intumescence) (3) Long-Photoperiod Injury (4) Light Spectral Quality Effects (5) Super-Elevated CO2 Injuries (6) Ethylene (7) Other Disorders (8) Considerations for Closed Space Environments. Views of plant with the disorders are shown

Visualizing Spacetime Curvature via Frame-Drag Vortexes and Tidal Tendexes I. General Theory and Weak-Gravity Applications

When one splits spacetime into space plus time, the Weyl curvature tensor (vacuum Riemann tensor) gets split into two spatial, symmetric, and trace-free (STF) tensors: (i) the Weyl tensor's so-called "electric" part or tidal field, and (ii) the Weyl tensor's so-called "magnetic" part or frame-drag field. Being STF, the tidal field and frame-drag field each have three orthogonal eigenvector fields which can be depicted by their integral curves. We call the integral curves of the tidal field's eigenvectors tendex lines, we call each tendex line's eigenvalue its tendicity, and we give the name tendex to a collection of tendex lines with large tendicity. The analogous quantities for the frame-drag field are vortex lines, their vorticities, and vortexes. We build up physical intuition into these concepts by applying them to a variety of weak-gravity phenomena: a spinning, gravitating point particle, two such particles side by side, a plane gravitational wave, a point particle with a dynamical current-quadrupole moment or dynamical mass-quadrupole moment, and a slow-motion binary system made of nonspinning point particles. [Abstract is abbreviated; full abstract also mentions additional results.]Comment: 25 pages, 20 figures, matches the published versio

Schroedinger's cat and the clock: Lessons for quantum gravity

I review basic principles of the quantum mechanical measurement process in view of their implications for a quantum theory of general relativity. It turns out that a clock as an external classical device associated with the observer plays an essential role. This leads me to postulate a ``principle of the integrity of the observer''. It essentially requires the observer to be part of a classical domain connected throughout the measurement process. Mathematically this naturally leads to a formulation of quantum mechanics as a kind of topological quantum field theory. Significantly, quantities with a direct interpretation in terms of a measurement process are associated only with amplitudes for connected boundaries of compact regions of space-time. I discuss some implications of my proposal such as in-out duality for states, delocalization of the ``collapse of the wave function'' and locality of the description. Differences to existing approaches to quantum gravity are also highlighted.Comment: 12 pages, 3 figures, LaTeX + AMS + eps; introduction, section numbers and two references adde