TriticeaeSSRdb: a comprehensive database of simple sequence repeats in Triticeae

Microsatellites, known as simple sequence repeats (SSRs), are short tandem repeats of 1 to 6 nucleotide motifs found in all genomes, particularly eukaryotes. They are widely used as co-dominant markers in genetic analyses and molecular breeding. Triticeae, a tribe of grasses, includes major cereal crops such as bread wheat, barley, and rye, as well as abundant forage and lawn grasses, playing a crucial role in global food production and agriculture. To enhance genetic work and expedite the improvement of Triticeae crops, we have developed TriticeaeSSRdb, an integrated and user-friendly database. It contains 3,891,705 SSRs from 21 species and offers browsing options based on genomic regions, chromosomes, motif types, and repeat motif sequences. Advanced search functions allow personalized searches based on chromosome location and length of SSR. Users can also explore the genes associated with SSRs, design customized primer pairs for PCR validation, and utilize practical tools for whole-genome browsing, sequence alignment, and in silico SSR prediction from local sequences. We continually update TriticeaeSSRdb with additional species and practical utilities. We anticipate that this database will greatly facilitate trait genetic analyses and enhance molecular breeding strategies for Triticeae crops. Researchers can freely access the database at http://triticeaessrdb.com/

Mapping the global flows of steel scraps: an alloy elements recovery perspective

Recycling steel scraps by the use of electric arc furnace is one of the most promising approaches for the steel industry to achieve net-zero emissions. Due to the uneven distribution of global steel scraps, many countries are actively involving in the global steel scraps trade. Steel scraps contain a range of critical elements, which may be transferred across borders through international trade of steel scraps. However, existing studies have paid little attention to the global flows of steel scraps and its embodied alloy elements (AEs). This study maps the journey of global steel scraps and the embodied AEs for the period of 2000–2021 for the first time by employing trade-linked material flow analysis and social network analysis. The results indicate that the global steel scraps trade had increased during the study period, with a few core countries (such as USA, Germany, and Turkey) leading the global steel scraps network. Also, critical metals had been transferred across borders in the form of AEs through the trade of steel scraps, especially from global north countries to global south countries. The largest AE flows include Chromium (Cr), nickel (Ni), manganese (Mn) and molybdenum (Mo) flows. Other AE flows, such as cobalt (Co), vanadium (V), and niobium (Nb) flows, were less, but with high values or being regarded scarce. From a global perspective, steel scraps trade and recycling can contribute to the decarbonization efforts of the global steel industry and address resource shortages in some countries. Therefore, it is urgent to promote the overall resource efficiency of steel scraps and the embodied AEs by various efforts

Lanosterol reverses protein aggregation in cataracts

The human lens is comprised largely of crystallin proteins assembled into a highly ordered, interactive macro-structure essential for lens transparency and refractive index. Any disruption of intra- or inter-protein interactions will alter this delicate structure, exposing hydrophobic surfaces, with consequent protein aggregation and cataract formation. Cataracts are the most common cause of blindness worldwide, affecting tens of millions of people1, and currently the only treatment is surgical removal of cataractous lenses. The precise mechanisms by which lens proteins both prevent aggregation and maintain lens transparency are largely unknown. Lanosterol is an amphipathic molecule enriched in the lens. It is synthesized by lanosterol synthase (LSS) in a key cyclization reaction of a cholesterol synthesis pathway. Here we identify two distinct homozygous LSS missense mutations (W581R and G588S) in two families with extensive congenital cataracts. Both of these mutations affect highly conserved amino acid residues and impair key catalytic functions of LSS. Engineered expression of wild-type, but not mutant, LSS prevents intracellular protein aggregation of various cataract-causing mutant crystallins. Treatment by lanosterol, but not cholesterol, significantly decreased preformed protein aggregates both in vitro and in cell-transfection experiments. We further show that lanosterol treatment could reduce cataract severity and increase transparency in dissected rabbit cataractous lenses in vitro and cataract severity in vivo in dogs. Our study identifies lanosterol as a key molecule in the prevention of lens protein aggregation and points to a novel strategy for cataract prevention and treatment