369 research outputs found
Comment on Mie Scattering from a Sonoluminescing Bubble with High Spatial and Temporal Resolution [Physical Review E 61, 5253 (2000)]
A key parameter underlying the existence of sonoluminescence (SL)is the time
relative to SL at which acoustic energy is radiated from the collapsed bubble.
Light scattering is one route to this quantity. We disagree with the statement
of Gompf and Pecha that -highly compressed water causes the minimum in
scattered light to occur 700ps before SL- and that this effect leads to an
overestimate of the bubble wall velocity. We discuss potential artifacts in
their experimental arrangement and correct their description of previous
experiments on Mie scattering.Comment: 10 pages, 2 figure
Neutrophil gelatinase associated lipocalin (NGAL) is elevated in type 2 diabetics with carotid artery stenosis and reduced under metformin treatment
Abstract Background Neutrophil gelatinase-associated lipocalin (NGAL), an acute phase protein released by neutrophils, has been described as biomarker of inflammatory states. Type 2 diabetes mellitus (T2DM) is characterized by increased inflammation and an elevated risk for embolization of carotid artery stenosis (CAS). We aimed to explore the role of NGAL systemically and in plaques of diabetics undergoing carotid endarterectomy. Moreover, the potential anti-inflammatory effect of metformin on NGAL was addressed in diabetics. Methods Serum NGAL and matrix metalloproteinase (MMP)-9/NGAL levels were measured in 136 patients (67 with T2DM vs. 69 non-diabetics) by specific ELISA. Endarterectomy samples were graded histologically according to the American Heart Association´s classification. NGAL mRNA expression was detected using RealTime-PCR in carotid endarterectomy specimens. Results Serum NGAL [median 107.4 ng/ml (quartiles: 75.2–145.0) vs. 64.4 (50.4 –81.3), p < 0.0001] and MMP-9/NGAL [41.5 ng/ml (20.8–63.9) vs. 27.6 (16.0–42.4), p = 0.017] were significantly elevated in diabetics compared to non-diabetics, as were leukocytes, neutrophils, C-reactive protein and fibrinogen (all p < 0.05). In patients with symptomatic and asymptomatic CAS diabetics had higher NGAL levels compared to non-diabetics [128.8 ng/ml (100.8–195.6) vs. 64.8 (48.9–82.2] and [101.6 ng/ml (70.1–125.3) vs. 63.8 (51.0–81.3), respectively, both p < 0.0001]. Presence of T2DM and type VI plaques (with surface defect, hemorrhage or thrombus) had a profound impact on NGAL levels (both p < 0.01) in multiple linear regression analysis. NGAL mRNA was detectable in 95% of analyzed carotid artery lesions of diabetics compared to 5% of non-diabetics (p < 0.0001). Accordingly, cerebral embolization was more frequent in diabetics (52.2% vs. 29%, p = 0.006). Metformin treatment was associated with decreased NGAL [60.7 ng/ml (51.9–69.2) vs. 121.7 (103.7–169.9), p < 0.0001] and MMP-9/NGAL [20.8 ng/ml (12.1–26.5) vs. 53.7 (27.4–73.4), p = 0.007] in diabetics and reduced leukocyte infiltration in carotid lesions of diabetics. Conclusions Higher NGAL levels in serum and plaques are associated with T2DM in patients with CAS. Metformin significantly reduced the inflammatory burden including NGAL in diabetics. Early treatment of these patients may be recommended, as elevated NGAL levels were linked with vulnerable plaques prone for embolization
Mechanisms for Stable Sonoluminescence
A gas bubble trapped in water by an oscillating acoustic field is expected to
either shrink or grow on a diffusive timescale, depending on the forcing
strength and the bubble size. At high ambient gas concentration this has long
been observed in experiments. However, recent sonoluminescence experiments show
that in certain circumstances when the ambient gas concentration is low the
bubble can be stable for days. This paper presents mechanisms leading to
stability which predict parameter dependences in agreement with the
sonoluminescence experiments.Comment: 4 pages, 3 figures on request (2 as .ps files
An Alternative Method to Deduce Bubble Dynamics in Single Bubble Sonoluminescence Experiments
In this paper we present an experimental approach that allows to deduce the
important dynamical parameters of single sonoluminescing bubbles (pressure
amplitude, ambient radius, radius-time curve) The technique is based on a few
previously confirmed theoretical assumptions and requires the knowledge of
quantities such as the amplitude of the electric excitation and the phase of
the flashes in the acoustic period. These quantities are easily measurable by a
digital oscilloscope, avoiding the cost of expensive lasers, or ultrafast
cameras of previous methods. We show the technique on a particular example and
compare the results with conventional Mie scattering. We find that within the
experimental uncertainties these two techniques provide similar results.Comment: 8 pages, 5 figures, submitted to Phys. Rev.
Spinal cord from body donors is suitable for multicolor immunofluorescence
Immunohistochemistry is a powerful tool for studying neuronal tissue from humans at the molecular level. Obtaining fresh neuronal tissue from human organ donors is difficult and sometimes impossible. In anatomical body donations, neuronal tissue is dedicated to research purposes and because of its easier availability, it may be an alternative source for research. In this study, we harvested spinal cord from a single organ donor 2 h (h) postmortem and spinal cord from body donors 24, 48, and 72 h postmortem and tested how long after death, valid multi-color immunofluorescence or horseradish peroxidase (HRP) immunohistochemistry is possible. We used general and specific neuronal markers and glial markers for immunolabeling experiments. Here we showed that it is possible to visualize molecularly different neuronal elements with high precision in the body donor spinal cord 24 h postmortem and the quality of the image data was comparable to those from the fresh organ donor spinal cord. High-contrast multicolor images of the 24-h spinal cords allowed accurate automated quantification of different neuronal elements in the same sample. Although there was antibody-specific signal reduction over postmortem intervals, the signal quality for most antibodies was acceptable at 48 h but no longer at 72 h postmortem. In conclusion, our study has defined a postmortem time window of more than 24 h during which valid immunohistochemical information can be obtained from the body donor spinal cord. Due to the easier availability, neuronal tissue from body donors is an alternative source for basic and clinical research
Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants
Mutations and inadequate methylation profiles of CITED2 are associated with human congenital heart disease (CHD). In mouse, Cited2 is necessary for embryogenesis, particularly for heart development, and its depletion in embryonic stem cells (ESC) impairs cardiac differentiation. We have now determined that Cited2 depletion in ESC affects the expression of transcription factors and cardiopoietic genes involved in early mesoderm and cardiac specification. Interestingly, the supplementation of the secretome prepared from ESC overexpressing CITED2, during the onset of differentiation, rescued the cardiogenic defects of Cited2-depleted ESC. In addition, we demonstrate that the proteins WNT5A and WNT11 held the potential for rescue. We also validated the zebrafish as a model to investigate cited2 function during development. Indeed, the microinjection of morpholinos targeting cited2 transcripts caused developmental defects recapitulating those of mice knockout models, including the increased propensity for cardiac defects and severe death rate. Importantly, the co-injection of anti-cited2 morpholinos with either CITED2 or WNT5A and WNT11 recombinant proteins corrected the developmental defects of Cited2-morphants. This study argues that defects caused by the dysfunction of Cited2 at early stages of development, including heart anomalies, may be remediable by supplementation of exogenous molecules, offering the opportunity to develop novel therapeutic strategies aiming to prevent CHD.Agência financiadora:
Fundação para a Ciência e a Tecnologia (FCT)
Comissão de Coordenação e Desenvolvimento Regional do Algarve (CCDR Algarve)
ALG-01-0145-FEDER-28044; DFG 568/17-2 Algarve Biomedical Center (ABC)
Municipio de Louléinfo:eu-repo/semantics/publishedVersio
Identifying source populations for the reintroduction of the Eurasian beaver, Castor fiber L. 1758, into Britain: evidence from ancient DNA
The file attached is the Published/publisher’s pdf version of the article
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