741 research outputs found
Establishment of mouse and rat hepatoma cell clones showing stable expression of rabbit cytochrome P450 IA2
AbstractCytochrome P450 IA2, a liver-specific member of the 3-methylcholanthrene-inducible family, is never detected in established cell lines. With the aim of isolating cells stably producing this protein, we have used rat and mouse hepatoma cells as recipients in transfection experiments involving rabbit cytochrome P450 IA2 cDNA. We report here the isolation of five hepatoma cell clones expressing functional P450 IA2. The level of expression is comparable to that found in COS cells transiently transformed by other P450 cDNAs. It ranges between 0.4 and 1.6 pmol P450 IA2/mg total cell protein
Conjugate gradient algorithms and the Galerkin boundary element method
Original article can be found at: http://www.sciencedirect.com/science/journal/08981221 Copyright Elsevier Ltd. DOI: 10.1016/j.camwa.2004.02.002Peer reviewe
Detection of serum antibodies and circulating antigens in a chimpanzee experimentally infected with Onchocerca volvulus
The course of the humoral immune response was followed in a chimpanzee experimentally infected over 27 weeks with a total of 168 Onchocerca volvulus 3rd-stage larvae obtained from naturally infected wild-caught blackflies. Antibodies against an adult worm extract could be detected by ELISA from week 16 onwards (after the inoculation of 44 larvae). Peak antibody levels were observed between weeks 66 and 74 (about one year after the last larval injection). Thereafter, antibody levels markedly decreased but rose again after week 120. First microfilariae could be detected from week 124 onwards. Microfilarial counts remained low (not more than two microfilariae per skin snip) until the end of the observation period. High levels of IgM antibodies against adult O. volvulus antigens were detectable between weeks 26 and 80 by ELISA. Total IgE levels were found to be only marginally elevated during the course of the infection. Circulating parasite antigens were only detectable for a short time (weeks 34 to 44) of the prepatent period by immuno-radiometric assays (IRMAs) using monoclonal antibodies which were raised against O. gibsoni eggs. Competitive radio-immuno-assays detected host antibodies inhibiting binding of 125I-labelled monoclonal antibodies to parasite antigens from week 28 onwards. Host antibodies clearly interfere later in infection with the detection of circulating antigen
Phenology and temperature‐dependent development of Ceutorhynchus assimilis, a potential biological control agent for Lepidium draba
Lepidium draba (Brassicaceae) is a major concern for agriculture and biodiversity in the western United States. As current control methods do not provide long-term, sustainable solutions, research has been conducted to find biological control agents. Ceutorhynchus assimilis is one of the currently investigated candidates. Known as oligophagous in the literature, a specialist clade of this root-galling weevil exists in southern Europe. This raised the question of its ability to survive in colder climates in the target range. We investigated the phenology of C. assimilis in the field in southern France (specialist clade) and Romania (generalist clade) and measured various temperature-dependent parameters in the laboratory. In both ranges, weevils were univoltine. Oviposition in autumn started later in France compared to Romania, while mature larvae exited galls (to pupate in the soil) earlier the following year. On average, 25% and 32% of galls from France and Romania were completely below the soil surface, respectively, and this appeared to depend on soil substrate. Weevils transported from France to Romania were able to develop, but at a much lower rate than Romanian weevils. Mortality of overwintering larvae of both clades increased with decreasing temperature and exposure time. At −5°C, lethal times Lt50 and Lt95 were 15 and 42 days for the specialist clade and 26 and 72 days for the generalist clade. A higher proportion of third instar larvae compared to first and second instar larvae survived. Pupation time at different temperatures did not differ between weevils from France or Romania. A climate match model (comparing winter temperatures) indicated that the specialist clade of C. assimilis from France has the potential to establish in some parts of the target range (e.g. Washington, Oregon, California). However, temperature extremes and winters without snow cover will likely limit its establishment unless rapid adaptive evolution takes place
Sign-reversal of drag in bilayer systems with in-plane periodic potential modulation
We develop a theory for describing frictional drag in bilayer systems with
in-plane periodic potential modulations, and use it to investigate the drag
between bilayer systems in which one of the layers is modulated in one
direction. At low temperatures, as the density of carriers in the modulated
layer is changed, we show that the transresistivity component in the direction
of modulation can change its sign. We also give a physical explanation for this
behavior.Comment: 4 pages, 4 figure
Spatial Patterns Induced Purely by Dichotomous Disorder
We study conditions under which spatially extended systems with coupling a la
Swift-Hohenberg exhibit spatial patterns induced purely by the presence of
quenched dichotomous disorder. Complementing the theoretical results based on a
generalized mean-field approximation, we also present numerical simulations of
particular dynamical systems that exhibit the proposed phenomenology
Magnetic and charge structures in itinerant-electron magnets: Coexistence of multiple SDW and CDW
A theory of Kondo lattices is applied to studying possible magnetic and
charge structures of itinerant-electron antiferromagnets. Even helical spin
structures can be stabilized when the nesting of the Fermi surface is not sharp
and the superexchange interaction, which arises from the virtual exchange of
pair excitations across the Mott-Hubbard gap, is mainly responsible for
magnetic instability. Sinusoidal spin structures or spin density waves (SDW)
are only stabilized when the nesting of the Fermi surface is sharp enough and a
novel exchange interaction arising from that of pair excitations of
quasi-particles is mainly responsible for magnetic instability. In particular,
multiple SDW are stabilized when their incommensurate ordering wave-numbers
are multiple; magnetizations of different components
are orthogonal to each other in double and triple SDW when magnetic anisotropy
is weak enough. Unless are commensurate, charge density waves
(CDW) with coexist with SDW with . Because the
quenching of magnetic moments by the Kondo effect depends on local numbers of
electrons, the phase of CDW or electron densities is such that magnetic moments
are large where the quenching is weak. It is proposed that the so called stipe
order in cuprate-oxide high-temperature superconductors must be the coexisting
state of double incommensurate SDW and CDW.Comment: 10 pages, no figure
Frequency-dependent magnetotransport and particle dynamics in magnetic modulation systems
We analyze the dynamics of a charged particle moving in the presence of
spatially-modulated magnetic fields. From Poincare surfaces of section and
Liapunov exponents for characteristic trajectories we find that the fraction of
pinned and runaway quasiperiodic orbits {\em vs}. chaotic orbits depends
strongly on the ratio of cyclotron radius to the structure parameters, as well
as on the amplitude of the modulated field. We present a complete
characterization of the dynamical behavior of such structures, and investigate
the contribution to the magnetoconductivity from all different orbits using a
classical Kubo formula. Although the DC conductivity of the system depends
strongly on the pinned and runaway trajectories, the frequency response
reflects the topology of all different orbits, and even their unusual temporal
behavior.Comment: Submitted to PRB - 14 figure files - REVTEX tex
Motion integration using competitive priors
Psychophysical experiments show that humans are better at perceiving rotation and expansion than translation [5][9]. These findings are inconsistent with standard models of motion integration which predict best performance for translation. To explain this discrepancy, our theory formulates motion perception at two levels of inference: we first perform model selection between the competing models (e.g. translation, rotation, and expansion) and then estimate the velocity using the selected model. We define novel prior models for smooth rotation and expansion using techniques similar to those in the slow-and-smooth model [23] (e.g. Green functions of differential operators). The theory gives good agreement with the trends observed in four human experiments
Weak-Localization in Chaotic Versus Non-Chaotic Cavities: A Striking Difference in the Line Shape
We report experimental evidence that chaotic and non-chaotic scattering
through ballistic cavities display distinct signatures in quantum transport. In
the case of non-chaotic cavities, we observe a linear decrease in the average
resistance with magnetic field which contrasts markedly with a Lorentzian
behavior for a chaotic cavity. This difference in line-shape of the
weak-localization peak is related to the differing distribution of areas
enclosed by electron trajectories. In addition, periodic oscillations are
observed which are probably associated with the Aharonov-Bohm effect through a
periodic orbit within the cavities.Comment: 4 pages revtex + 4 figures on request; amc.hub.94.
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