Infection Characteristics of Rice Stripe Mosaic Virus in the Body of the Vector Leafhoppers

Rice stripe mosaic virus (RSMV), a novel species of Cytorhabdovirus, is transmitted by the leafhopper Recilia dorsalis in a persistent-propagative manner. In this study, we firstly confirmed that N protein of RSMV is a component of viroplasm and virion in vector culture cells of R. dorsalis. Confocal microscopy revealed that RSMV initially accumulated in epithelial cells of the filter chamber of R. dorsalis, from where it proceeded to the visceral muscles surrounding the filter chamber. Subsequently, RSMV spread quickly throughout the suspensory ligament to the salivary glands. Meanwhile, RSMV spread from the filter chamber to midgut, hindgut, esophagus, hemolymph, and central nervous system. We further observed that RSMV particles displayed as non-enveloped form when propagating in cytoplasm of different tissues, and became enveloped when spread within insect body by electron microscopy. Additionally, we found that the leafhopper Nephotettix virescens was also able to acquire and transmit RSMV. These results clarified the infection characteristics of RSMV in its leafhopper vectors, which will help guide the formulation of RSMV prevention and control strategies

Rice Yellow Stunt Nucleorhabdovirus Matrix Protein Mediates Viral Axonal Transport in the Central Nervous System of Its Insect Vector

Persistently transmitted plant viruses encounter multiple membrane and tissue barriers in the process of completing their infection routes within their insect vectors. Some of these viruses have been reported to overcome the elaborate barriers of the central nervous system (CNS) to travel through the nervous tissues, but the specific mechanisms of this process remain unknown. Here, we report the axonal transport mechanism of rice yellow stunt virus (RYSV), a nucleorhabdovirus, in the CNS of the green rice leafhopper (Nephotettix cincticeps). The infection route of RYSV in the internal organs of its insect vector after ingestion of the virus was investigated by immunofluorescence microscopy. RYSV was first detected in the epithelial cells of midgut regions, from where it proceeded to the nervous system, and finally into the salivary glands. We then utilized immunofluorescence and electron microscopy to investigate the distribution of RYSV particles within the leafhopper CNS, demonstrating that non-enveloped viral particles distributed along the microtubule-based neurofilaments in the axon cytoplasm following the direct interaction of leafhopper α-tubulin with the RYSV M protein. Tubulin inhibitors inhibited the dissemination of RYSV to the CNS, then into the salivary glands in leafhoppers. We therefore describe a mechanism of plant virus transport through CNS axons as an alternative means of rapid viral dissemination in an insect vector

Exploration of an Actin Promoter-Based Transient Expression Vector to Trace the Cellular Localization of Nucleorhabdovirus Proteins in Leafhopper Cultured Cells

Continuously cultured cell lines derived from planthopper and leafhopper have greatly facilitated the investigation of rice viruses transmitted by these insects. However, the lack of a suitable transient expression vector has limited their utility. Here, by cloning and analyzing the promoter sequence of the gene encoding cytoplasmic actin from the leafhopper Nephotettix cincticeps, we successfully developed the first efficient transient expression vector for cultured leafhopper cells, which can also be used to express exogenous proteins in other insect culture cell lines, including those derived from Recilia dorsalis leafhopper and Spodoptera frugiperda (Sf9). Furthermore, insertion of the Hr5 viral enhancer element and knockdown of the endogenous Dicer2 gene notably improved the vector’s expression efficiency in leafhopper cells. Using the optimized vector, we have for the first time traced the cellular localization of the proteins encoded by rice yellow stunt virus (RYSV) in cells of its insect vector and demonstrated that P6 protein is a component of the viroplasm

Formation of Complexes at Plasmodesmata for Potyvirus Intercellular Movement Is Mediated by the Viral Protein P3N-PIPO

Intercellular transport of viruses through cytoplasmic connections, termed plasmodesmata (PD), is essential for systemic infection in plants by viruses. Previous genetic and ultrastructural data revealed that the potyvirus cyclindrical inclusion (CI) protein is directly involved in cell-to-cell movement, likely through the formation of conical structures anchored to and extended through PD. In this study, we demonstrate that plasmodesmatal localization of CI in N. benthamiana leaf cells is modulated by the recently discovered potyviral protein, P3N-PIPO, in a CI:P3N-PIPO ratio-dependent manner. We show that P3N-PIPO is a PD-located protein that physically interacts with CI in planta. The early secretory pathway, rather than the actomyosin motility system, is required for the delivery of P3N-PIPO and CI to PD. Moreover, CI mutations that disrupt virus cell-to-cell movement compromise PD-localization capacity. These data suggest that the CI and P3N-PIPO complex coordinates the formation of PD-associated structures that facilitate the intercellular movement of potyviruses in infected plants

ICTV virus taxonomy profile : Sedoreoviridae 2022

Sedoreoviridae is a large family of icosahedral viruses that are usually regarded as non- enveloped with segmented (10–12 linear segments) dsRNA genomes of 18–26 kbp. Sedoreovirids have a broad host range, infecting mammals, birds, crustaceans, arthropods, algae and plants. Some of them have important pathogenic potential for humans (e.g. rotavirus A), livestock (e.g. bluetongue virus) and plants (e.g. rice dwarf virus).Instituto de BiotecnologíaFil: Matthijnssens, Jelle. University of Leuven; BélgicaFil: Attoui, Houssam. National Institute for Agricultural Research (INRA); FranciaFil: Bányai, Krisztián. Veterinary Medical Research Institute; HungríaFil: Brussaard, Corina P. D. NIOZ Royal Netherlands Institute for Sea Research; Países BajosFil: Brussaard, Corina P. D. University of Utrecht; Países BajosFil: Danthi, Pranav. Indiana University; Estados UnidosFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Dermody, Terence S. University of Pittsburgh. School of Medicine; Estados UnidosFil: Duncan, Roy. Dalhousie University; CanadáFil: Fāng, Qín. Wuhan Institute of Virology; ChinaFil: Johne, Reimar. German Federal Institute for Risk Assessment; AlemaniaFil: Mertens, Peter P. C. University of Nottingham; Reino UnidoFil: Jaafar, Fauziah Mohd. Ecole Nationale Vétérinaire d’Alfort; FranciaFil: Patton, John T. Indiana University; Estados UnidosFil: Sasaya, Takahide. National Agriculture and Food Research Organization; JapónFil: Suzuki, Nobuhiro. Okayama University. JapónFil: Wei, Taiyun. Fujian Agriculture and Forestry University; Chin

Formation of Complexes at Plasmodesmata for Potyvirus Intercellular Movement Is Mediated by the Viral Protein P3N-PIPO

Intercellular transport of viruses through cytoplasmic connections, termed plasmodesmata (PD), is essential for systemic infection in plants by viruses. Previous genetic and ultrastructural data revealed that the potyvirus cyclindrical inclusion (CI) protein is directly involved in cell-to-cell movement, likely through the formation of conical structures anchored to and extended through PD. In this study, we demonstrate that plasmodesmatal localization of CI in N. benthamiana leaf cells is modulated by the recently discovered potyviral protein, P3N-PIPO, in a CI:P3N-PIPO ratio-dependent manner. We show that P3N-PIPO is a PD-located protein that physically interacts with CI in planta. The early secretory pathway, rather than the actomyosin motility system, is required for the delivery of P3N-PIPO and CI to PD. Moreover, CI mutations that disrupt virus cell-to-cell movement compromise PD-localization capacity. These data suggest that the CI and P3N-PIPO complex coordinates the formation of PD-associated structures that facilitate the intercellular movement of potyviruses in infected plants

Taxonomy of the order Bunyavirales : second update 2018

In October 2018, the order Bunyavirales was amended by inclusion of the family Arenaviridae, abolishment of three families, creation of three new families, 19 new genera, and 14 new species, and renaming of three genera and 22 species. This article presents the updated taxonomy of the order Bunyavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).Non peer reviewe