Construction of Readership in \u3cem\u3eEbony\u3c/em\u3e, \u3cem\u3eEssence\u3c/em\u3e, and \u3cem\u3eO, the Oprah Magazine\u3c/em\u3e

Miller et al examine the construction of readership in Ebony, Essence and O, The Oprah magazine, three popular magazines that purport to be a vehicle of identity and awareness for their target audience. Upon evaluation, they found that Ebony and Essence both challenge the hegemonic process with the incorporation of cultural artifacts that call upon collective memory to form reader association

Meltwater sources and sinks for multiyear Arctic sea ice in summer

On Arctic sea ice, the melt of snow and sea ice generate a summertime flux of fresh water to the upper ocean. The partitioning of this meltwater to storage in melt ponds and deposition in the ocean has consequences for the surface heat budget, the sea ice mass balance, and primary productivity. Synthesizing results from the 1997-1998 SHEBA field experiment, we calculate the sources and sinks of meltwater produced on a multiyear floe during summer melt. The total meltwater input to the system from snowmelt, ice melt, and precipitation from 1 June to 9 August was equivalent to a layer of water 80ĝ€¯cm thick over the ice-covered and open ocean. A total of 85ĝ€¯% of this meltwater was deposited in the ocean, and only 15ĝ€¯% of this meltwater was stored in ponds. The cumulative contributions of meltwater input to the ocean from drainage from the ice surface and bottom melting were roughly equal

An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?

Reliable diagnosis of human helminth infection(s) is essential for ongoing disease surveillance and disease elimination. Current WHO-recommended diagnostic assays are unreliable in low-endemic near-elimination settings and typically involve the invasive, onerous and potentially hazardous sampling of bodily fluids such as stool and blood, as well as tissue via biopsy. In contrast, diagnosis by use of non-invasive urine sampling is generally painless, more convenient and low risk. It negates the need for specialist staff, can usually be obtained immediately upon request and is better accepted by patients. In some instances, urine-based diagnostic assays have also been shown to provide a more reliable diagnosis of infection when compared to traditional methods that require alternative and more invasive bodily samples, particularly in low-endemicity settings. Given these relative benefits, we identify and review current research literature to evaluate whether non-invasive urine sampling is currently exploited to its full potential in the development of diagnostic tools for human helminthiases. Though further development, assessment and validation is needed before their routine use in control programmes, low-cost, rapid and reliable assays capable of detecting transrenal helminth-derived antigens and cell-free DNA show excellent promise for future use at the point-of-care in high-, medium- and even low-endemicity elimination settings

Development of a lateral flow recombinase polymerase assay for the diagnosis of Schistosoma mansoni infections.

Infection with Schistosoma mansoni causes intestinal schistosomiasis, a major health problem across Africa. The accurate diagnosis of intestinal schistosomiasis is vital to inform surveillance/control programs. Diagnosis mainly relies on microscopic detection of eggs in faecal samples but many factors affect sensitivity. Molecular diagnostics are sensitive and specific but application is limited as necessary infrastructure, financial resources and skilled personnel are often lacking in endemic settings. Recombinase Polymerase Amplification (RPA) is an isothermal DNA amplification/detection technology that is practical in nearly any setting. Here we developed a RPA lateral flow (LF) assay targeting the 28S rDNA region of S. mansoni. The 28S LF-RPA assay's lower limit of detection was 10pg DNA with the lower test parameters permitting sufficient amplification being 6 min and 25°C. Optimal assay parameters were 40-45°C and 10 min with an analytical sensitivity of 102 copies of DNA. Additionally the PCRD3 lateral flow detection cassettes proved more robust and sensitive compared to the Milenia HybriDetect strips. This 28S LF-RPA assay produces quick reproducible results that are easy to interpret, require little infrastructure and is a promising PON test for the field molecular diagnosis of intestinal schistosomiasis

Future schistosome hybridizations: Will all Schistosoma haematobium hybrids please stand-up!

nterrogating the genetic make-up of schistosome larvae (i.e. eggs, miracidia and cercariae) originating from definitive or intermediate snail hosts with molecular DNA methods has, by noting unexpected inter-species hybrids, started a revolution in our appraisal of African schistosomiasis [1-4]. Here, two dominant species of human schistosome exist, Schistosoma haematobium and S. mansoni, which are transmitted by specific intermediate freshwater snails, Bulinus spp. for the former and Biomphalaria spp. for the latter. The two schistosomes cause either urogenital or intestinal schistosomiasis, respectively [5] and depending on local snail distributions, schistosome transmission zones in the aquatic habitat may or may not overlap [6]. Within the S. haematobium group, a further 8 sister species are described with S. intercalatum and S. guineensis of medical importance, causing intestinal schistosomiasis while others, such as S. bovis, S. curassoni and S. mattheei occur in livestock, with the remaining species infecting wildlife. Schistosoma mattheei is also of medical interest for occasional infection and associated disease [7]. In contrast, S. mansoni has a single sister species, S. rodhaini, typically found in small rodents which can hybridise with S. mansoni, if given sufficient opportunity [2]

The influence of snow on sea ice as assessed from simulations of CESM2

We assess the influence of snow on sea ice in experiments using the Community Earth System Model version 2 for a preindustrial and a 2xCO2 climate state. In the preindustrial climate, we find that increasing simulated snow accumulation on sea ice results in thicker sea ice and a cooler climate in both hemispheres. The sea ice mass budget response differs fundamentally between the two hemispheres. In the Arctic, increasing snow results in a decrease in both congelation sea ice growth and surface sea ice melt due to the snow\u27s impact on conductive heat transfer and albedo, respectively. These factors dominate in regions of perennial ice but have a smaller influence in seasonal ice areas. Overall, the mass budget changes lead to a reduced amplitude in the annual cycle of ice thickness. In the Antarctic, with increasing snow, ice growth increases due to snow-ice formation and is balanced by larger basal ice melt, which primarily occurs in regions of seasonal ice. In a warmer 2xCO2 climate, the Arctic sea ice sensitivity to snow depth is small and reduced relative to that of the preindustrial climate. In contrast, in the Antarctic, the sensitivity to snow on sea ice in the 2xCO2 climate is qualitatively similar to the sensitivity in the preindustrial climate. These results underscore the importance of accurately representing snow accumulation on sea ice in coupled Earth system models due to its impact on a number of competing processes and feedbacks that affect the melt and growth of sea ice

Epidemiological Interactions between Urogenital and Intestinal Human Schistosomiasis in the Context of Praziquantel Treatment across Three West African Countries

© 2015 Knowles et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The attached file is the published version of the article

The substructure of three repetitive DNA regions of Schistosoma haematobium group species as a potential marker for species recognition and interbreeding detection

The file attached is the Published/publisher’s pdf version of the article.© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated