2,634 research outputs found
Growth and survival of nearshore fishes in Lake Michigan
Illinois Department of Natural resources Grant/Contract No: F-138INHS Technical Report Prepared for Illinois Department of Natural resource
Who Inhabits a Business Ecosystem? The Technospecies as a Unifying Concept
Currently, many terms are used to describe business ecosystems and their inhabitants. These terms have meanings that can cause definitional confusion and an ambiguous level of analysis as to what constitutes a business ecosystem. To understand business ecosystem relationships, an unambiguous understanding of the ecosystem components is required. The importance of standardized terminology and clear definitions of these components has been recognized in the literature. From a managerial perspective, identifying the relationships a firm is situated in is valuable and useful information that can be practically applied. We propose a business ecosystem model anchored around interdependent technospecies similar to the biological model that many of the existing concepts are drawn from. Technospecies are unique entities based on their organizational routines, capabilities, and use of technology. This article will present an alternative formulation of the business ecosystem model with the aim of synthesizing the diverse terminology presently in use into a concise, common language
The failure of microglia to digest developmental apoptotic cells contributes to the pathology of RNASET2-deficient leukoencephalopathy
The contribution of microglia in neurological disorders is emerging as a leading disease driver rather than a consequence of pathology. RNAseT2‐deficient leukoencephalopathy is a severe childhood white matter disorder affecting patients in their first year of life and mimicking a cytomegalovirus brain infection. The early onset and resemblance of the symptoms to a viral infection suggest an inflammatory and embryonic origin of the pathology. There are no treatments available for this disease as our understanding of the cellular drivers of the pathology are still unknown. In this study, using a zebrafish mutant for the orthologous rnaset2 gene, we have identified an inflammatory signature in early development and an antiviral immune response in mature adult brains. Using the optical transparency and the ex utero development of the zebrafish larvae we studied immune cell behavior during brain development and identified abnormal microglia as an early marker of pathology. Live imaging and electron microscopy identified that mutant microglia displayed an engorged morphology and were filled with undigested apoptotic cells and undigested substrate. Using microglia‐specific depletion and rescue experiments, we identified microglia as drivers of this embryonic phenotype and potential key cellular player in the pathology of RNAseT2‐deficient leukoencephalopathy. Our zebrafish model also presented with reduced survival and locomotor defects, therefore recapitulating many aspects of the human disease. Our study therefore placed our rnaset2 mutant at the forefront of leukodystrophy preclinical models and highlighted tissue‐specific approaches as future therapeutic avenues
2-Arachidonoylglycerol mobilizes myeloid cells and worsens heart function after acute myocardial infarction.
Myocardial infarction (MI) leads to an enhanced release of endocannabinoids and a massive accumulation of neutrophils and monocytes within the ischaemic myocardium. These myeloid cells originate from haematopoietic precursors in the bone marrow and are rapidly mobilized in response to MI. We aimed to determine whether endocannabinoid signalling is involved in myeloid cell mobilization and cardiac recruitment after ischaemia onset.
Intravenous administration of endocannabinoid 2-arachidonoylglycerol (2-AG) into wild type (WT) C57BL6 mice induced a rapid increase of blood neutrophil and monocyte counts as measured by flow cytometry. This effect was blunted when using cannabinoid receptor 2 knockout mice. In response to MI induced in WT mice, the lipidomic analysis revealed significantly elevated plasma and cardiac levels of the endocannabinoid 2-AG 24 h after infarction, but no changes in anandamide, palmitoylethanolamide, and oleoylethanolamide. This was a consequence of an increased expression of 2-AG synthesizing enzyme diacylglycerol lipase and a decrease of metabolizing enzyme monoacylglycerol lipase (MAGL) in infarcted hearts, as determined by quantitative RT-PCR analysis. The opposite mRNA expression pattern was observed in bone marrow. Pharmacological blockade of MAGL with JZL184 and thus increased systemic 2-AG levels in WT mice subjected to MI resulted in elevated cardiac CXCL1, CXCL2, and MMP9 protein levels as well as higher cardiac neutrophil and monocyte counts 24 h after infarction compared with vehicle-treated mice. Increased post-MI inflammation in these mice led to an increased infarct size, an impaired ventricular scar formation assessed by histology and a worsened cardiac function in echocardiography evaluations up to 21 days. Likewise, JZL184-administration in a myocardial ischaemia-reperfusion model increased cardiac myeloid cell recruitment and resulted in a larger fibrotic scar size.
These findings suggest that changes in endocannabinoid gradients due to altered tissue levels contribute to myeloid cell recruitment from the bone marrow to the infarcted heart, with crucial consequences on cardiac healing and function
The time-of-day of myocardial infarction onset affects healing through oscillations in cardiac neutrophil recruitment
Myocardial infarction (MI) is the leading cause of death in Western countries. Epidemiological studies show acute MI to be more prevalent in the morning and to be associated with a poorer outcome in terms of mortality and recovery. The mechanisms behind this association are not fully understood. Here, we report that circadian oscillations of neutrophil recruitment to the heart determine infarct size, healing, and cardiac function after MI Preferential cardiac neutrophil recruitment during the active phase (Zeitgeber time, ZT13) was paralleled by enhanced myeloid progenitor production, increased circulating numbers of CXCR2(hi) neutrophils as well as upregulated cardiac adhesion molecule and chemokine expression. MI at ZT13 resulted in significantly higher cardiac neutrophil infiltration compared to ZT5, which was inhibited by CXCR2 antagonism or neutrophil-specific CXCR2 knockout. Limiting exaggerated neutrophilic inflammation at this time point significantly reduced the infarct size and improved cardiac function
Extramedullary Hematopoiesis Generates Ly-6C(high) Monocytes That Infiltrate Atherosclerotic Lesions
BACKGROUND: Atherosclerotic lesions are believed to grow via the recruitment of bone marrow-derived monocytes. Among the known murine monocyte subsets, Ly-6C(high) monocytes are inflammatory, accumulate in lesions preferentially, and differentiate. Here we hypothesized that the bone marrow outsources the production of Ly-6C(high) monocytes during atherosclerosis. METHODS AND RESULTS: Using murine models of atherosclerosis and fate-mapping approaches, we show that hematopoietic stem and progenitor cells (HSPC) progressively relocate from the bone marrow to the splenic red pulp where they encounter GM-CSF and IL-3, clonally expand, and differentiate to Ly-6C(high) monocytes. Monocytes born in such extramedullary niches intravasate, circulate, and accumulate abundantly in atheromata. Upon lesional infiltration, Ly-6C(high) monocytes secrete inflammatory cytokines, reactive oxygen species, and proteases. Eventually, they ingest lipids and become foam cells. CONCLUSIONS: Our findings indicate that extramedullary sites supplement the bone marrow’s hematopoietic function by producing circulating inflammatory cells that infiltrate atherosclerotic lesions
The rp-process and new measurements of beta-delayed proton decay of light Ag and Cd isotopes
Recent network calculations suggest that a high temperature rp-process could
explain the abundances of light Mo and Ru isotopes, which have long challenged
models of p-process nuclide production. Important ingredients to network
calculations involving unstable nuclei near and at the proton drip line are
-halflives and decay modes, i.e., whether or not -delayed proton
decay takes place. Of particular importance to these network calculation are
the proton-rich isotopes Ag, Ag, Cd and Cd. We
report on recent measurements of -delayed proton branching ratios for
Ag, Ag, and Cd at the on-line mass separator at GSI.Comment: 4 pages, uses espcrc1.sty. Proceedings of the 4th International
Symposium Nuclei in the Cosmos, June 1996, Notre Dame/IN, USA, Ed. M.
Wiescher, to be published in Nucl.Phys.A. Also available at
ftp://ftp.physics.ohio-state.edu/pub/nucex/nic96-gs
Very High Resolution Solar X-ray Imaging Using Diffractive Optics
This paper describes the development of X-ray diffractive optics for imaging
solar flares with better than 0.1 arcsec angular resolution. X-ray images with
this resolution of the \geq10 MK plasma in solar active regions and solar
flares would allow the cross-sectional area of magnetic loops to be resolved
and the coronal flare energy release region itself to be probed. The objective
of this work is to obtain X-ray images in the iron-line complex at 6.7 keV
observed during solar flares with an angular resolution as fine as 0.1 arcsec -
over an order of magnitude finer than is now possible. This line emission is
from highly ionized iron atoms, primarily Fe xxv, in the hottest flare plasma
at temperatures in excess of \approx10 MK. It provides information on the flare
morphology, the iron abundance, and the distribution of the hot plasma.
Studying how this plasma is heated to such high temperatures in such short
times during solar flares is of critical importance in understanding these
powerful transient events, one of the major objectives of solar physics. We
describe the design, fabrication, and testing of phase zone plate X-ray lenses
with focal lengths of \approx100 m at these energies that would be capable of
achieving these objectives. We show how such lenses could be included on a
two-spacecraft formation-flying mission with the lenses on the spacecraft
closest to the Sun and an X-ray imaging array on the second spacecraft in the
focal plane \approx100 m away. High resolution X-ray images could be obtained
when the two spacecraft are aligned with the region of interest on the Sun.
Requirements and constraints for the control of the two spacecraft are
discussed together with the overall feasibility of such a formation-flying
mission
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