Spatial control of irreversible protein aggregation

Liquid cellular compartments spatially segregate from the cytoplasm and can regulate aberrant protein aggregation, a process linked to several medical conditions, including Alzheimer's and Parkinson's diseases. Yet the mechanisms by which these droplet-like compartments affect protein aggregation remain unknown. Here, we combine kinetic theory of protein aggregation and liquid-liquid phase separation to study the spatial control of irreversible protein aggregation in the presence of liquid compartments. We find that, even for weak interactions between the compartment constituents and the aggregating monomers, aggregates are strongly enriched inside the liquid compartment relative to the surrounding cytoplasm. We show that this enrichment is caused by a positive feedback mechanism of aggregate nucleation and growth which is mediated by a flux maintaining the phase equilibrium between the compartment and the cytoplasm. Our model predicts that the compartment volume that maximizes aggregate enrichment in the compartment is determined by the reaction orders of aggregate nucleation. The underlying mechanism of aggregate enrichment could be used to confine cytotoxic protein aggregates inside droplet-like compartments suggesting potential new avenues against aberrant protein aggregation. Our findings could also represent a common mechanism for the spatial control of irreversible chemical reactions in general

The Use of Multi-beam Sonars to Image Bubbly Ship Wakes

During the past five years, researchers at Penn State University (PSU) have used upward-looking multi-beam (MB) sonar to image the bubbly wakes of surface ships. In 2000, a 19-beam, 5° beam width, 120° sector, 250 kHz MB sonar integrated into an autonomous vehicle was used to obtain a first-of-a-kind look at the three-dimensional variability of bubbles in a large ship wake. In 2001 we acquired a Reson 8101 MB sonar, which operates at 240 kHz and features 101-1.5º beams spanning a 150º sector. In July 2002, the Reson sonar was deployed looking upward from a 1.4 m diameter buoy moored at 29.5 m depth in 550 m of water using three anchor lines. A fiber optic cable connected the sonar to a support ship 500 m away. Images of the wake of a small research vessel provided new information about the persistence of bubble clouds in the ocean. An important goal is to use the MB sonar to estimate wake bubble distributions, as has been done with single beam sonar. Here we show that multipath interference and strong, specular reflections from the sea surface adversely affect the use of MB sonars to unambiguously estimate wake bubble distributio

Consistency in statistical moments as a test for bubble cloud clustering

Frequency dependent measurements of attenuation and/or sound speed through clouds of gas bubbles in liquids are often inverted to find the bubble size distribution and the void fraction of gas. The inversions are often done using an effective medium theory as a forward model under the assumption that the bubble positions are Poisson distributed (i.e., statistically independent). Under circumstances in which single scattering does not adequately describe the pressure field, the assumption of independence in position can yield large errors when clustering is present, leading to errors in the inverted bubble size distribution. It is difficult, however, to determine the existence of clustering in bubble clouds without the use of specialized acoustic or optical imaging equipment. A method is described here in which the existence of bubble clustering can be identified by examining the consistency between the first two statistical moments of multiple frequency acoustic measurements

Ku-band radar threshold analysis

The statistics of the CFAR threshold for the Ku-band radar was determined. Exact analytical results were developed for both the mean and standard deviations in the designated search mode. The mean value is compared to the results of a previously reported simulation. The analytical results are more optimistic than the simulation results, for which no explanation is offered. The normalized standard deviation is shown to be very sensitive to signal-to-noise ratio and very insensitive to the noise correlation present in the range gates of the designated search mode. The substantial variation in the CFAR threshold is dominant at large values of SNR where the normalized standard deviation is greater than 0.3. Whether or not this significantly affects the resulting probability of detection is a matter which deserves additional attention

Supersonic-combustion rocket

A supersonic combustion rocket is provided in which a small rocket motor is substituted for heavy turbo pumps in a conventional rocket engine. The substitution results in a substantial reduction in rocket engine weight. The flame emanating from the small rocket motor can act to ignite non-hypergolic fuels

What Difference do Bystanders Make? The Association of Bystander Involvement with Victim Outcomes in a Community Sample

Objective: To fill gaps in the bystander literature by describing patterns of bystander involvement and associations between bystander involvement and victim outcomes across different types of emotional, physical, and sexual victimizations and to expand these considerations to a rural rather than urban sample. Method: Adults and adolescents (n = 1,703) were surveyed about bystander actions, bystander safety, and victim outcomes (injury, disrupted routine, fear level, and current mental health) for 10 forms of victimization. Results: Bystanders were present for roughly 2 thirds of most victimization types (59% to 67%), except sexual victimization (17%). Relatives were the most common bystanders of family violence and friends or acquaintances were the most common bystanders of peer violence. For all 10 victimizations, more bystanders helped than harmed the situation, but most commonly had no impact. Rates of bystander harm for sexual victimizations were higher than for other types. Especially for peer-perpetrated incidents, victim outcomes were often better when bystanders helped. Bystander safety (unharmed and unthreatened) was consistently associated with better victim outcomes. Conclusion: Bystanders witness the majority of physical and psychological victimizations. These data lend support to the premise of many prevention programs that helpful bystanders are associated with more positive victim outcomes. Bystander prevention should focus on the type of bystanders most commonly present and should teach bystanders ways to stay safe while helping victims

Space Shuttle Proximity Operation Sensor Study

The performance of the Kuband radar was analyzed in detail, and the performance was updated and summarized. In so doing, two different radar design philosophies were described, and the corresponding differences in losses were enumerated. The resulting design margins were determined for both design philosophies and for both the designated and nondesignated range modes of operation. In some cases, the design margin was about zero, and in other cases it was significantly less than zero. With the point of view described above, the recommended solution is to allow more scan time but at the present scan rate. With no other changes in the present configuration, the radar met design detection specifications for all design philosophies at a range of 11.3 nautical miles

Performance Boost in Industrial Multifilamentary Nb3Sn Wires due to Radiation Induced Pinning Centers

We report non-Cu critical current densities of 4.09 * 10^9 A/m^2 at 12 T and 2.27 * 10^9 A/m^2 at 15 T obtained from transport measurements on a Ti-alloyed RRP Nb3Sn wire after irradiation to a fast neutron fluence of 8.9 * 10^21 m^-2. These values are to our knowledge unprecedented in multifilamentary Nb3Sn, and they correspond to a Jc enhancement of approximately 60% relative to the unirradiated state. Our magnetometry data obtained on short wire samples irradiated to fast neutron fluences of up to 2.5 * 10^22 m^-2 indicate the possibility of an even better performance, whereas earlier irradiation studies on bronze-processed Nb3Sn wires with a Sn content further from stoichiometry attested a decline of the critical current density at such high fluences. We show that radiation induced point-pinning centers rather than an increase of the upper critical field are responsible for this Jc enhancement, and argue that these results call for further research on pinning landscape engineering.Comment: 6 pages, 4 figures; to be published in Scientific Report

Effects of Neutron Irradiation on Pinning Force Scaling in State-of-the-Art Nb3Sn Wires

We present an extensive irradiation study involving five state-of-the-art Nb3Sn wires which were subjected to sequential neutron irradiation up to a fast neutron fluence of 1.6 * 10^22 m^-2 (E > 0.1 MeV). The volume pinning force of short wire samples was assessed in the temperature range from 4.2 to 15 K in applied fields of up to 7 T by means of SQUID magnetometry in the unirradiated state and after each irradiation step. Pinning force scaling computations revealed that the exponents in the pinning force function differ significantly from those expected for pure grain boundary pinning, and that fast neutron irradiation causes a substantial change in the functional dependence of the volume pinning force. A model is presented, which describes the pinning force function of irradiated wires using a two-component ansatz involving a point-pinning contribution stemming from radiation induced pinning centers. The dependence of this point-pinning contribution on fast neutron fluence appears to be a universal function for all examined wire types.Comment: 8 page

Study to investigate and evaluate means of optimizing the Ku-band combined radar/communication functions for the space shuttle

The performance of the space shuttle orbiter's Ku-Band integrated radar and communications equipment is analyzed for the radar mode of operation. The block diagram of the rendezvous radar subsystem is described. Power budgets for passive target detection are calculated, based on the estimated values of system losses. Requirements for processing of radar signals in the search and track modes are examined. Time multiplexed, single-channel, angle tracking of passive scintillating targets is analyzed. Radar performance in the presence of main lobe ground clutter is considered and candidate techniques for clutter suppression are discussed. Principal system parameter drivers are examined for the case of stationkeeping at ranges comparable to target dimension. Candidate ranging waveforms for short range operation are analyzed and compared. The logarithmic error discriminant utilized for range, range rate and angle tracking is formulated and applied to the quantitative analysis of radar subsystem tracking loops