Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

The Site Tilt and Lander Transfer Function from the Short-Period Seismometer of InSight on Mars

The National Aeronautics and Space Administration InSight mission has deployed the seis- mic experiment, SEIS, on the surface of Mars, and has recorded a variety of signals includ- ing marsquakes and dust devils. This work presents results on the tilt and local noise sources, which provide context to aid interpretation of the observed signals and allow an examination of the near-surface properties. Our analysis uses data recorded by the short-period sensors on the deck, throughout deployment and in the final configuration. We use thermal decorrelation to provide an estimate of the sol-to-sol tilt. This tilt is exam- ined across deployment and over a Martian year. After each modification to the site, the tilt is seen to stabilize over 3–20 sols depending on the action, and the total change in tilt is < 0.035°. Long-term tilt over a Martian year is limited to < 0.007°. We also investigate the attenuation of lander-induced vibrations between the lander and SEIS. Robotic arm motions provide a known lander source in the 5–9 Hz bandwidth, yielding an amplitude attenuation of lander signals between 100 and 1000 times. The attenuation of wind sen- sitivity from the deck to ground presents a similar value in the 1.5–9 Hz range, thus favor- ing a noise dominated by lander vibrations induced by the wind. Wind sensitivities outside this bandwidth exhibit different sensitivity changes, indicating a change in the coupling. The results are interpreted through a finite-element analysis of the regolith with a depth- dependent Young’s modulus. We argue that discrepancies between this model and the observations are due to local compaction beneath the lander legs and/or anelasticity. An estimate for the effective Young’s modulus is obtained as 62–81 MPa, corroborating previous estimates for the top layer duricrust

Experimental Validation of Molecular Dynamics Simulations of Lipid Bilayers: A New Approach

A novel protocol has been developed for comparing the structural properties of lipid bilayers determined by simulation with those determined by diffraction experiments, which makes it possible to test critically the ability of molecular dynamics simulations to reproduce experimental data. This model-independent method consists of analyzing data from molecular dynamics bilayer simulations in the same way as experimental data by determining the structure factors of the system and, via Fourier reconstruction, the overall transbilayer scattering-density profiles. Multi-nanosecond molecular dynamics simulations of a dioleoylphosphatidylcholine bilayer at 66% RH (5.4 waters/lipid) were performed in the constant pressure and temperature ensemble using the united-atom GROMACS and the all-atom CHARMM22/27 force fields with the GROMACS and NAMD software packages, respectively. The quality of the simulated bilayer structures was evaluated by comparing simulation with experimental results for bilayer thickness, area/lipid, individual molecular-component distributions, continuous and discrete structure factors, and overall scattering-density profiles. Neither the GROMACS nor the CHARMM22/27 simulations reproduced experimental data within experimental error. The widths of the simulated terminal methyl distributions showed a particularly strong disagreement with the experimentally observed distributions. A comparison of the older CHARMM22 with the newer CHARMM27 force fields shows that significant progress is being made in the development of atomic force fields for describing lipid bilayer systems empirically

Vortex-Dominated Aeolian Activity at InSight's Landing Site, Part 1: Multi-Instrument Observations, Analysis, and Implications

We report the aeolian changes observed in situ by NASA's InSight lander during the first 400 sols of operations: Granule creep, saltation, dust removal, and the formation of dark surface tracks. Aeolian changes are infrequent and sporadic. However, on sols, when they do occur, they consistently appear between noon to 3 p.m., and are associated with the passage of convective vortices during periods of high vortex activity. Aeolian changes are more frequent at elevated locations, such as the top surfaces of rocks and lander footpads. InSight observed these changes using, for the first time, simultaneous in-situ and orbital imaging and high-frequency meteorological, seismological, and magnetic measurements. Seismometer measurements of ground acceleration constrain the timing and trajectory of convective vortex encounters, linking surface changes to source vortices. Magnetometer measurements show perturbations in magnetic field strength during the passage of convective vortices consistent with charged-particle motion. Detachment of sand-scale particles occurs when high background winds and vortex-induced turbulence provide a peak surface friction wind speed above the classic saltation fluid threshold. However, detachment of dust- and granule-scale particles also occurred when the surface friction wind speed remained below this threshold. This may be explained by local enhancement of the surface roughness and other effects described here and further studied in Part 2 (Baker et al., 2021). The lack of saltation and bright dust-coated surfaces at the InSight landing site implies surface stability and the onset of particle motion may be suppressed by dust “cushioning.” This differentiates the InSight landing site from other areas on Mars that exhibit more aeolian activity.ISSN:0148-0227ISSN:2169-909

Red noise versus planetary interpretations in the microlensing event Ogle-2013-BLG-446

For all exoplanet candidates, the reliability of a claimed detection needs to be assessed through a careful study of systematic errors in the data to minimize the false positives rate. We present a method to investigate such systematics in microlensing data sets using the microlensing event OGLE-2013-BLG-0446 as a case study. The event was observed from multiple sites around the world and its high magnification (Amax ~ 3000) allowed us to investigate the effects of terrestrial and annual parallax. Real-time modeling of the event while it was still ongoing suggested the presence of an extremely low-mass companion (~3M⨁) to the lensing star, leading to substantial follow-up coverage of the light curve. We test and compare different models for the light curve and conclude that the data do not favor the planetary interpretation when systematic errors are taken into account