322 research outputs found
Mining State-Based Models from Proof Corpora
Interactive theorem provers have been used extensively to reason about
various software/hardware systems and mathematical theorems. The key challenge
when using an interactive prover is finding a suitable sequence of proof steps
that will lead to a successful proof requires a significant amount of human
intervention. This paper presents an automated technique that takes as input
examples of successful proofs and infers an Extended Finite State Machine as
output. This can in turn be used to generate proofs of new conjectures. Our
preliminary experiments show that the inferred models are generally accurate
(contain few false-positive sequences) and that representing existing proofs in
such a way can be very useful when guiding new ones.Comment: To Appear at Conferences on Intelligent Computer Mathematics 201
Property-Based Testing - The ProTest Project
The ProTest project is an FP7 STREP on property based testing. The purpose of the project is to develop software engineering approaches to improve reliability of service-oriented networks; support fault-finding and diagnosis based on specified properties of the system. And to do so we will build automated tools that will generate and run tests, monitor execution at run-time, and log events for analysis.
The Erlang / Open Telecom Platform has been chosen as our initial implementation vehicle due to its robustness and reliability within the telecoms sector. It is noted for its success in the ATM telecoms switches by Ericsson, one of the project partners, as well as for multiple other uses such as in facebook, yahoo etc. In this paper we provide an overview of the project goals, as well as detailing initial progress in developing property based testing techniques and tools for the concurrent functional programming language Erlang
Electrically Small Supergain Arrays
The theory, computer simulations, and experimental measurements are presented
for electrically small two-element supergain arrays with near optimal endfire
gains of 7 dB. We show how the difficulties of narrow tolerances, large
mismatches, low radiation efficiencies, and reduced scattering of electrically
small parasitic elements are overcome by using electrically small resonant
antennas as the elements in both separately driven and singly driven
(parasitic) two-element electrically small supergain endfire arrays. Although
rapidly increasing narrow tolerances prevent the practical realization of the
maximum theoretically possible endfire gain of electrically small arrays with
many elements, the theory and preliminary numerical simulations indicate that
near maximum supergains are also achievable in practice for electrically small
arrays with three (and possibly more) resonant elements if the decreasing
bandwidth with increasing number of elements can be tolerated.Comment: 10 pages, 11 figures, submitted to IEEE Transactions on Antennas and
Propagation (December 2006
The AIQ Meta-Testbed: Pragmatically Bridging Academic AI Testing and Industrial Q Needs
AI solutions seem to appear in any and all application domains. As AI becomes
more pervasive, the importance of quality assurance increases. Unfortunately,
there is no consensus on what artificial intelligence means and interpretations
range from simple statistical analysis to sentient humanoid robots. On top of
that, quality is a notoriously hard concept to pinpoint. What does this mean
for AI quality? In this paper, we share our working definition and a pragmatic
approach to address the corresponding quality assurance with a focus on
testing. Finally, we present our ongoing work on establishing the AIQ
Meta-Testbed.Comment: Accepted for publication in the Proc. of the Software Quality Days
2021, Vienna, Austri
A new family of covalent inhibitors block nucleotide binding to the active site of pyruvate kinase
PYK (pyruvate kinase) plays a central role in the metabolism of many organisms and cell types, but the elucidation of the details of its function in a systems biology context has been hampered by the lack of specific high-affinity small-molecule inhibitors. High-throughput screening has been used to identify a family of saccharin derivatives which inhibit LmPYK (Leishmania mexicana PYK) activity in a time- (and dose-) dependent manner, a characteristic of irreversible inhibition. The crystal structure of DBS {4-[(1,1-dioxo-1,2-benzothiazol-3-yl)sulfanyl]benzoic acid} complexed with LmPYK shows that the saccharin moiety reacts with an active-site lysine residue (Lys335), forming a covalent bond and sterically hindering the binding of ADP/ATP. Mutation of the lysine residue to an arginine residue eliminated the effect of the inhibitor molecule, providing confirmation of the proposed inhibitor mechanism. This lysine residue is conserved in the active sites of the four human PYK isoenzymes, which were also found to be irreversibly inhibited by DBS. X-ray structures of PYK isoforms show structural differences at the DBS-binding pocket, and this covalent inhibitor of PYK provides a chemical scaffold for the design of new families of potentially isoform-specific irreversible inhibitors
Curcumin-induced HDAC inhibition and attenuation of medulloblastoma growth in vitro and in vivo
<p>Abstract</p> <p>Background</p> <p>Medulloblastoma is the most common brain tumor in children, and its prognosis is worse than for many other common pediatric cancers. Survivors undergoing treatment suffer from serious therapy-related side effects. Thus, it is imperative to identify safer, effective treatments for medulloblastoma. In this study we evaluated the anti-cancer potential of curcumin in medulloblastoma by testing its ability to induce apoptosis and inhibit tumor growth <it>in vitro </it>and <it>in vivo </it>using established medulloblastoma models.</p> <p>Methods</p> <p>Using cultured medulloblastoma cells, tumor xenografts, and the Smo/Smo transgenic medulloblastoma mouse model, the antitumor effects of curcumin were tested <it>in vitro </it>and <it>in vivo</it>.</p> <p>Results</p> <p>Curcumin induced apoptosis and cell cycle arrest at the G2/M phase in medulloblastoma cells. These effects were accompanied by reduced histone deacetylase (HDAC) 4 expression and activity and increased tubulin acetylation, ultimately leading to mitotic catastrophe. In <it>in vivo </it>medulloblastoma xenografts, curcumin reduced tumor growth and significantly increased survival in the Smo/Smo transgenic medulloblastoma mouse model.</p> <p>Conclusions</p> <p>The <it>in vitro </it>and <it>in vivo </it>data suggest that curcumin has the potential to be developed as a therapeutic agent for medulloblastoma.</p
Protective Effect of Tetrahydroxystilbene Glucoside on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway
Oxidative stress plays an important role in the pathogenesis of neurodegenerative diseases, such as Parkinson's disease. The molecule, 2,3,5,4′-tetrahydr- oxystilbene-2-O-β-D-glucoside (TSG), is a potent antioxidant derived from the Chinese herb, Polygonum multiflorum Thunb. In this study, we investigated the protective effect of TSG against 6-hydroxydopamine-induced apoptosis in rat adrenal pheochromocytoma PC12 cells and the possible mechanisms. Our data demonstrated that TSG significantly reversed the 6-hydroxydopamine-induced decrease in cell viability, prevented 6-hydroxydopamine-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. In addition, TSG slowed the accumulation of intracellular reactive oxygen species and nitric oxide, counteracted the overexpression of inducible nitric oxide syntheses as well as neuronal nitric oxide syntheses, and also reduced the level of protein-bound 3-nitrotyrosine. These results demonstrate that the protective effects of TSG on rat adrenal pheochromocytoma PC12 cells are mediated, at least in part, by the ROS-NO pathway. Our results indicate that TSG may be effective in providing protection against neurodegenerative diseases associated with oxidative stress
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