1,746 research outputs found
Healthy volunteersâ perceptions of risk in US Phase I clinical trials: A mixed-methods study
Schroedinger cat-like states by conditional measurements on a beam-splitter
A scheme for generating Schr\"{o}dinger cat-like states of a single-mode
optical field by means of conditional measurement is proposed. Feeding into a
beam splitter a squeezed vacuum and counting the photons in one of the output
channels, the conditional states in the other output channel exhibit a number
of properties that are very similar to those of superpositions of two coherent
states with opposite phases. We present analytical and numerical results for
the photon-number and quadrature-component distributions of the conditional
states and their Wigner and Husimi functions. Further, we discuss the effect of
realistic photocounting on the states.Comment: 6 figures(divided in subfigures) using a4.st
Aromatase Inhibition Reduces Insulin Sensitivity in Healthy Men
CONTEXT: Deficiency of aromatase, the enzyme that catalyzes the conversion of androgens to estrogens, is associated with insulin resistance in humans and mice. OBJECTIVE: We hypothesized that pharmacological aromatase inhibition results in peripheral insulin resistance in humans. DESIGN: This was a double-blind, randomized, controlled, crossover study. SETTING: The study was conducted at a clinical research facility. PARTICIPANTS: Seventeen healthy male volunteers (18â50 y) participated in the study. INTERVENTION: The intervention included oral anastrozole (1 mg daily) and placebo, each for 6 weeks with a 2-week washout period. MAIN OUTCOME MEASURE: Glucose disposal and rates of lipolysis were measured during a stepwise hyperinsulinemic euglycemic clamp. Data are mean (SEM). RESULTS: Anastrozole therapy resulted in significant estradiol suppression (59.9 ± 3.6 vs 102.0 ± 5.7 pmol/L, P = < .001) and a more modest elevation of total T (25.8 ± 1.2 vs 21.4 ± 0.7 nmol/L, P = .003). Glucose infusion rate, during the low-dose insulin infusion, was lower after anastrozole administration (12.16 ± 1.33 vs 14.15 ± 1.55 ÎŒmol/kg·min, P = .024). No differences in hepatic glucose production or rate of lipolysis were observed. CONCLUSION: Aromatase inhibition reduces insulin sensitivity, with respect to peripheral glucose disposal, in healthy men. Local generation and action of estradiol, at the level of skeletal muscle, is likely to be an important determinant of insulin sensitivity
Comparison of three different diets for organic broilers: effects on performance and body condition
"Dark energy" in the Local Void
The unexpected discovery of the accelerated cosmic expansion in 1998 has
filled the Universe with the embarrassing presence of an unidentified "dark
energy", or cosmological constant, devoid of any physical meaning. While this
standard cosmology seems to work well at the global level, improved knowledge
of the kinematics and other properties of our extragalactic neighborhood
indicates the need for a better theory. We investigate whether the recently
suggested repulsive-gravity scenario can account for some of the features that
are unexplained by the standard model. Through simple dynamical considerations,
we find that the Local Void could host an amount of antimatter
() roughly equivalent to the mass of a typical
supercluster, thus restoring the matter-antimatter symmetry. The antigravity
field produced by this "dark repulsor" can explain the anomalous motion of the
Local Sheet away from the Local Void, as well as several other properties of
nearby galaxies that seem to require void evacuation and structure formation
much faster than expected from the standard model. At the global cosmological
level, gravitational repulsion from antimatter hidden in voids can provide more
than enough potential energy to drive both the cosmic expansion and its
acceleration, with no need for an initial "explosion" and dark energy.
Moreover, the discrete distribution of these dark repulsors, in contrast to the
uniformly permeating dark energy, can also explain dark flows and other
recently observed excessive inhomogeneities and anisotropies of the Universe.Comment: 6 pages, accepted as a Letter to the Editor by Astrophysics and Space
Scienc
Pion photoproduction on the nucleon in the quark model
We present a detailed quark-model study of pion photoproduction within the
effective Lagrangian approach. Cross sections and single-polarization
observables are investigated for the four charge channels, , , , and .
Leaving the coupling strength to be a free parameter, we obtain a
reasonably consistent description of these four channels from threshold to the
first resonance region. Within this effective Lagrangian approach, strongly
constrainted by the quark model, we consider the issue of double-counting which
may occur if additional {\it t}-channel contributions are included.Comment: Revtex, 35 pages, 16 eps figures; version to appear on PR
Genetic characterisation of Escherichia coli RecN protein as a member of SMC family of proteins
YesThe proteins of SMC family are characterised by having Walker A and B sites. The Escherichia coli RecN protein is a prokaryotic member of SMC family that involved in the induced excision of Tn10 and the repair of the DNA double strand breaks. In this work, the Walker A nucleotide binding site of the E. coli RecN protein was mutated by changing the highly conserved lysine residue 35 to the aspartic acid (D), designated as recN(K35D). Reverse genetics was utilized to delete the entire recN gene (Delta recN108) or introduce the recN(K35D) gene into the E. coli chromosomal DNA. The recN(K35D) cells showed decreasing in the frequency of excision of Tn10 from gal7
Derivatization of estrogens enhances specificity and sensitivity of analysis of human plasma and serum by liquid chromatography tandem mass spectrometry
AbstractEstrogens circulate at concentrations less than 20pg/mL in men and postmenopausal women, presenting analytical challenges. Quantitation by immunoassay is unreliable at these low concentrations. Liquid chromatography tandem mass spectrometry (LCâMS/MS) offers greater specificity and sometimes greater sensitivity, but ionization of estrogens is inefficient. Introduction of charged moieties may enhance ionization, but many such derivatives of estrogens generate non-specific product ions originating from the âreagentâ group. Therefore an approach generating derivatives with product ions specific to individual estrogens was sought.Estrogens were extracted from human plasma and serum using solid phase extraction and derivatized using 2-fluoro-1-methylpyridinium-p-toluenesulfonate (FMP-TS). Electrospray in positive mode with multiple reaction monitoring using a QTrap 5500 mass spectrometer was used to quantify âFMPâ derivatives of estrogens, following LC separation.Transitions for the FMP derivatives of estrone (E1) and estradiol (E2) were compound specific (m/z 362â238 and m/z 364â128, respectively). The limits of detection and quantitation were 0.2pg on-column and the method was linear from 1â400pg/sample. Measures of intra- and inter-assay variability, precision and accuracy were acceptable (<20%). The derivatives were stable over 24h at 10°C (7â9% degradation). Using this approach, E1 and E2, respectively were detected in human plasma and serum: pre-menopausal female serum (0.5mL) 135â473, 193â722pmol/L; male plasma (1mL) 25â111, 60â180pmol/L and post-menopausal female plasma (2mL), 22â78, 29â50pmol/L.Thus FMP derivatization, in conjunction with LCâMS/MS, is suitable for quantitative analysis of estrogens in low abundance in plasma and serum, offering advantages in specificity over immunoassay and existing MS techniques
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