The interrater reliability of static palpation of the thoracic spine for eliciting tenderness and stiffness to test for a manipulable lesion

Background Despite widespread use by manual therapists, there is little evidence regarding the reliability of thoracic spine static palpation to test for a manipulable lesion using stiffness or tenderness as diagnostic markers. We aimed to determine the interrater agreement of thoracic spine static palpation for segmental tenderness and stiffness and determine the effect of standardised training for examiners. The secondary aim was to explore expert consensus on the level of segmental tenderness required to locate a “manipulable lesion”. Methods Two experienced chiropractors used static palpation of thoracic vertebrae on two occasions (pragmatic and standardised approaches). Participants rated tenderness on an 11-point numerical pain rating scale (NPRS) and raters judged segmental stiffness based on their experience and perception of normal mobility with the requested outcomes of hypomobile or normal mobility. We calculated interrater agreement using percent agreement, Cohen’s Kappa coefficients (κ) and prevalence-adjusted bias-adjusted Kappa coefficients (PABAK). In a preliminary study, an expert panel of 10 chiropractors took part in a Delphi process to identify the level of meaningful segmental tenderness required to locate a “manipulable lesion”. Results Thirty-six participants (20 female) were enrolled for the reliability study on the 13th March 2017. Mean (SD) age was 22.4 (3.4) years with an equal distribution of asymptomatic (n = 17) and symptomatic (n = 17) participants. Overall, the interrater agreement for spinal segmental stiffness had Kappa values indicating less than chance agreement [κ range − 0.11, 0.53]. When adjusted for prevalence and bias, the PABAK ranged from slight to substantial agreement [0.12–0.76] with moderate or substantial agreement demonstrated at the majority of spinal levels (T1, T2 and T6 to T12). Generally, there was fair to substantial agreement for segmental tenderness [Kappa range 0.22–0.77]. Training did not significantly improve interrater agreement for stiffness or tenderness. The Delphi process indicated that an NPRS score of 2 out of 10 identified a potential “manipulable lesion”. Conclusion Static palpation was overall moderately reliable for the identification of segmental thoracic spine stiffness and tenderness, with tenderness demonstrating a higher reliability. Also, an increased agreement was found within the mid-thoracic spine. A brief training intervention failed to improve reliability

Data on the number and frequency of scientific literature citations for established medulloblastoma cell lines

This article collates information about the number of scientific articles mentioning each of the established medulloblastoma cell lines, derived through a systematic search of Web of Science, Scopus and Google Scholar in 2016. The data for each cell line have been presented as raw number of citations, percentage share of the total citations for each search engine and as an average percentage between the three search engines. In order to correct for the time since each cell line has been in use, the raw citation data have also been divided by the number of years since the derivation of each cell line. This is a supporting article for a review of in vitro models of medulloblastoma published in “in vitro models of medulloblastoma: choosing the right tool for the job” (D.P. Ivanov, D.A. Walker, B. Coyle, A.M. Grabowska, 2016) [1]

Potential risk factors and triggers for back pain in children and young adults. A scoping review, part I: Incident and episodic back pain

Background The one-month prevalence of back pain in children and adolescents has been reported at 33, 28 and 48% at ages 9, 13 and 15 respectively. There are many suspected risk factors and triggers of back pain in young people. Objective The purpose of this scoping review was to identify potential risk factors and potential triggers for back pain in young people. The purpose of part I was to identify potential risk factors for incident and episodic back pain in young people. Part II included all eligible studies with unclear or mixed types of back pain. Methods Due to the vast number of studies on “risk factors” for back pain, a two-part scoping review of the literature was chosen as the best way to summarise the evidence. We adhered to the PRISMA-ScR guideline for scoping reviews. General potential risk factors and triggers for back pain in children and young adults (≤ 24 years) were included, incorporating physical, environmental, and/or physiological factors. A search was conducted using PubMed and Cochrane databases from inception to September 2018, limited to the English language. Within part I, and because of their importance, only the results of the studies that investigated risk factors of incident back pain and back pain episodes are presented. Results The search identified 7356 articles, of which 91 articles were eligible for this scoping review. The majority of the eligible articles had an unclear definition of back pain (results presented in scoping review part II). There were 7 inception cohort studies included and 1 cohort study that met the criteria for part I. The most consistent risk factors for incident and episodic back pain are female sex and older age. Conclusion Due to inconsistent ways of reporting on the type of back pain, no definitive risk factor for back pain has been identified. In general, females often report more symptoms, also for other diseases, and older age is not a useful risk factor as it merely indicates that the onset may not be in childhood. Clearly, the time has come to study the causes of back pain from different angles

An evaluation of ocean color model estimates of marine primary productivity in coastal and pelagic regions across the globe

Nearly half of the earth\u27s photosynthetically fixed carbon derives from the oceans. To determine global and region specific rates, we rely on models that estimate marine net primary productivity (NPP) thus it is essential that these models are evaluated to determine their accuracy. Here we assessed the skill of 21 ocean color models by comparing their estimates of depth-integrated NPP to 1156 in situ C-14 measurements encompassing ten marine regions including the Sargasso Sea, pelagic North Atlantic, coastal Northeast Atlantic, Black Sea, Mediterranean Sea, Arabian Sea, subtropical North Pacific, Ross Sea, West Antarctic Peninsula, and the Antarctic Polar Frontal Zone. Average model skill, as determined by root-mean square difference calculations, was lowest in the Black and Mediterranean Seas, highest in the pelagic North Atlantic and the Antarctic Polar Frontal Zone, and intermediate in the other six regions. The maximum fraction of model skill that may be attributable to uncertainties in both the input variables and in situ NPP measurements was nearly 72%. On average, the simplest depth/wavelength integrated models performed no worse than the more complex depth/wavelength resolved models. Ocean color models were not highly challenged in extreme conditions of surface chlorophyll-a and sea surface temperature, nor in high-nitrate low-chlorophyll waters. Water column depth was the primary influence on ocean color model performance such that average skill was significantly higher at depths greater than 250 m, suggesting that ocean color models are more challenged in Case-2 waters (coastal) than in Case-1 (pelagic) waters. Given that in situ chlorophyll-a data was used as input data, algorithm improvement is required to eliminate the poor performance of ocean color NPP models in Case-2 waters that are close to coastlines. Finally, ocean color chlorophyll-a algorithms are challenged by optically complex Case-2 waters, thus using satellite-derived chlorophyll-a to estimate NPP in coastal areas would likely further reduce the skill of ocean color models

Assimilating bio-optical glider data during a phytoplankton bloom in the southern Ross Sea

The Ross Sea is a region characterized by high primary productivity in comparison to other Antarctic coastal regions, and its productivity is marked by considerable variability both spatially (1-50 km) and temporally (days to weeks). This variability presents a challenge for inferring phytoplankton dynamics from observations that are limited in time or space, which is often the case due to logistical limitations of sampling. To better understand the spatio-temporal variability in Ross Sea phytoplankton dynamics and to determine how restricted sampling may skew dynamical interpretations, high-resolution bio-optical glider measurements were assimilated into a one-dimensional biogeochemical model adapted for the Ross Sea. The assimilation of data from the entire glider track using the micro-genetic and local search algorithms in the Marine Model Optimization Testbed improves the model-data fit by similar to 50 %, generating rates of integrated primary production of 104 g C m(-2) yr(-1) and export at 200 m of 27 g C m(-2) yr(-1). Assimilating glider data from three different latitudinal bands and three different longitudinal bands results in minimal changes to the simulations, improves the model-data fit with respect to unassimilated data by similar to 35 %, and confirms that analyzing these glider observations as a time series via a one-dimensional model is reasonable on these scales. Whereas assimilating the full glider data set produces well-constrained simulations, assimilating subsampled glider data at a frequency consistent with cruise-based sampling results in a wide range of primary production and export estimates. These estimates depend strongly on the timing of the assimilated observations, due to the presence of high mesoscale variability in this region. Assimilating surface glider data subsampled at a frequency consistent with available satellite-derived data results in 40% lower carbon export, primarily resulting from optimized rates generating more slowly sinking diatoms. This analysis highlights the need for the strategic consideration of the impacts of data frequency, duration, and coverage when combining observations with biogeochemical modeling in regions with strong mesoscale variability

Effects of Adenosine on Inositol 1,4,5‐Trisphosphate Formation and Intracellular Calcium Changes in Formyl‐Met‐Leu‐Phe‐Stimulated Human Neutrophils

In the presence of adenosine, formyl‐Met‐Leu‐Phe‐stimulated human neutrophils show a greatly diminished production of superoxide anion. Analysis of changes in levels of intracellular calcium revealed that the immediate increase (occurring within seconds) in intracellular calcium following addition of formyl‐Met‐Leu‐Phe is not affected by the presence of adenosine, although there are significantly lower intracellular calcium levels during the late phase (occurring 1–4 min after addition of formyl‐Met‐Leu‐Phe). Consistent with these findings is the fact that adenosine does not affect the production of inositol 1,4,5‐trisphosphate in formyl‐Met‐Leu‐Phe‐stimulated neutrophils. These data suggest that the profound inhibitory effects of adenosine on superoxide responses in formyl‐Met‐Leu‐Phe‐stimulated neutrophils may be related to an action of adenosine occurring late in the sequence of events of signal transduction.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141119/1/jlb0281.pd

Correlated electron emission in laser-induced nonsequence double ionization of Helium

In this paper, we have investigated the correlated electron emission of the nonsequence double ionization (NSDI) in an intense linearly polarized field. The theoretical model we employed is the semiclassical rescattering model, the model atom we used is the helium. We find a significant correlation between magnitude and direction of the momentum of two emission electrons, and give a good explanation for this striking phenomenon by observing the classical collisional trajectories. We argue that this correlation phenomenon is universal in NSDI process, as revealed by the recent experiment on the argon.Comment: 4 pages, 3 figures, accepted for publication in Phys. Rev.

The ability of a host plant to associate with different symbiotic partners affects ectomycorrhizal functioning

Some plants that associate with ectomycorrhizal (ECM) fungi are also able to simultaneously establish symbiosis with other types of partners. The presence of alternative partners that may provide similar benefits may affect ECM functioning. Here we compared potential leucine-aminopeptidase (LA) and acid phosphatase (AP) enzyme activity (involved in N and P cycling, respectively) in ECM fungi of three hosts planted under the same conditions but differing in the type of partners: Pinus (ECM fungi only), Eucalyptus (ECM and arbuscular mycorrhizal -AM- fungi) and Acacia (ECM, AM fungi and rhizobial bacteria). We found that the ECM community on Acacia and Eucalyptus had higher potential AP activity than the Pinus community. The ECM community in Acacia also showed increased potential LA activity compared to Pinus. Morphotypes present in more than one host showed higher potential AP and LA activity when colonizing Acacia than when colonizing another host. Our results suggest that competition with AM fungi and rhizobial bacteria could promote increased ECM activity in Eucalyptus and Acacia. Alternatively, other host-related differences such as ECM community composition could also play a role. We found evidence for ECM physiological plasticity when colonizing different hosts, which might be key for adaptation to future climate scenarios.info:eu-repo/semantics/acceptedVersio

Absence of FMLP Receptors on Rat Macrophages

Although rat peritoneal neutrophils in the presence of cytochalasin B demonstrate superoxide (O2‐) responses to the chemotactic peptide N’‐formyl‐met‐leu‐phe (FMLP), neither elicited rat peritoneal macrophages nor rat alveolar macrophages show an O2‐response to FMLP (in the presence or absence of cytochalasin B), although a good O2‐response to opsonized zymosan is demonstrated by both types of macrophages. Using Fura‐2 loaded cells, peritoneal macrophages failed to show an increase in intracellular calcium after exposure to FMLP, f‐nor‐leu‐phe, F‐met‐met‐met, or F‐norleu‐leu‐phe‐norleu‐lys. FMLP also failed to induce elevations in intracellular calcium in alveolar macrophages. In 3H‐FMLP binding studies, the lack of responsiveness of peritoneal and alveolar macrophages was associated with the lack of FMLP receptors on these cell types, in striking contrast to the presence of functional receptors on rat neutrophils.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141351/1/jlb0600.pd