Obstacles to the diffusion of adaptation in the Rhein-Neckar region in Germany

Because the impacts of climate change are felt at the local level, we assess adaptation diffusion mechanisms (i.e., learning, competition, and emulation) among smaller big cities and medium-sized towns. Since the diffusion of adaptation has immediate spatial implications, we argue that local conditions play an important role in the diffusion process. The densely populated Rhein-Neckar Metropolitan Region in Germany is an ideal case for studying diffusion mechanisms with regard to adaptation. Using a rich data set of 28 interviews, we unpack the views of local actors such as mayors, city council politicians, city administration representatives, and representatives from regional agencies and identify factors influencing the diffusion of adaptation. We find limited or compromised diffusion due to insufficient knowledge about adaptation, competition between municipalities, and cooperation with cities outside the region. In addition, we find some (albeit limited) political will for adaptation. While some of the interviewed politicians considered making long-term investments in adaptation, most highlighted competing local issues and viewed adaptation with caution, illustrating adaptation’s lack of salience and social legitimacy. Indeed, one crucial finding was that housing and mobility are more important to a wide range of politicians and bureaucrats alike. By examining diffusion mechanisms at the subnational level, we combine theoretical perspectives from political science and geography to show how local decision-makers—in particular, politicians—influence the diffusion mechanisms of adaptation

Psychometric Evaluation and Design of Patient-Centered Communication Measures for Cancer Care Settings

Objective To evaluate the psychometric properties of questions that assess patient perceptions of patient-provider communication and design measures of patient-centered communication (PCC). Methods Participants (adults with colon or rectal cancer living in North Carolina) completed a survey at 2 to 3 months post-diagnosis. The survey included 87 questions in six PCC Functions: Exchanging Information, Fostering Health Relationships, Making Decisions, Responding to Emotions, Enabling Patient Self-Management, and Managing Uncertainty. For each Function we conducted factor analyses, item response theory modeling, and tests for differential item functioning, and assessed reliability and construct validity. Results Participants included 501 respondents; 46% had a high school education or less. Reliability within each Function ranged from 0.90 to 0.96. The PCC-Ca-36 (36-question survey; reliability=0.94) and PCC-Ca-6 (6-question survey; reliability=0.92) measures differentiated between individuals with poor and good health (i.e., known-groups validity) and were highly correlated with the HINTS communication scale (i.e., convergent validity). Conclusion This study provides theory-grounded PCC measures found to be reliable and valid in colorectal cancer patients in North Carolina. Future work should evaluate measure validity over time and in other cancer populations. Practice implications The PCC-Ca-36 and PCC-Ca-6 measures may be used for surveillance, intervention research, and quality improvement initiatives

The Perceived Stressors and Coping Skills of Graduate Students: A Developmental and Validation Study

This article outlines the development and validation of two instruments evaluating common stressors and coping skills as perceived by graduate counseling students. The review of the literature illustrated a need for the development of measures to provide empirical support in regard to the stressors and coping skills of graduate students in counseling programs. Exploratory factor analyses were applied to the two respective scales to evaluate the constructs. Recommendations and limitations are offered to further the development of psychometric properties within the scales

Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio–venous connections

Recent reports suggest that mammalian embryonic coronary endothelium (CoE) originates from the sinus venosus and ventricular endocardium. However, the contribution of extracardiac cells to CoE is thought to be minor and nonsignificant for coronary formation. Using classic (Wt1(Cre)) and previously undescribed (G2-Gata4(Cre)) transgenic mouse models for the study of coronary vascular development, we show that extracardiac septum transversum/proepicardium (ST/PE)-derived endothelial cells are required for the formation of ventricular coronary arterio-venous vascular connections. Our results indicate that at least 20% of embryonic coronary arterial and capillary endothelial cells derive from the ST/PE compartment. Moreover, we show that conditional deletion of the ST/PE lineage-specific Wilms' tumor suppressor gene (Wt1) in the ST/PE of G2-Gata4(Cre) mice and in the endothelium of Tie2(Cre) mice disrupts embryonic coronary transmural patterning, leading to embryonic death. Taken together, our results demonstrate that ST/PE-derived endothelial cells contribute significantly to and are required for proper coronary vascular morphogenesi

Divergent Evolutionary Histories of DNA Markers in a Hawaiian Population of the Coral Montipora capitata

We investigated intra- and inter-colony sequence variation in a population of the dom- inant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers

Genome analysis of the rice coral \u3cem\u3eMontipora capitata\u3c/em\u3e

Corals comprise a biomineralizing cnidarian, dinoflagellate algal symbionts, and associated microbiome of prokaryotes and viruses. Ongoing efforts to conserve coral reefs by identifying the major stress response pathways and thereby laying the foundation to select resistant genotypes rely on a robust genomic foundation. Here we generated and analyzed a high quality long-read based ~886 Mbp nuclear genome assembly and transcriptome data from the dominant rice coral, Montipora capitata from Hawai’i. Our work provides insights into the architecture of coral genomes and shows how they differ in size and gene inventory, putatively due to population size variation. We describe a recent example of foreign gene acquisition via a bacterial gene transfer agent and illustrate the major pathways of stress response that can be used to predict regulatory components of the transcriptional networks in M. capitata. These genomic resources provide insights into the adaptive potential of these sessile, long-lived species in both natural and human influenced environments and facilitate functional and population genomic studies aimed at Hawaiian reef restoration and conservation

Comparison of effectiveness of cefovecin, doxycycline, and amoxicillin for the treatment of experimentally induced early Lyme borreliosis in dogs

Background While Koch’s postulates have been fulfilled for Lyme disease; causing transient fever, anorexia and arthritis in young dogs; treatment of sero-positive dogs, especially asymptomatic animals, remains a topic of debate. To complicate this matter the currently recommended antibiotic treatments of Lyme Disease in dogs caused by Borrelia burgdorferi require daily oral administrations for 31 days or longer, which makes non-compliance a concern. Additionally, there is no approved veterinary antimicrobial for the treatment of Lyme Disease in dogs in the USA and few recommended treatments have been robustly tested. In vitro testing of cefovecin, a novel extended-spectrum cephalosporin, demonstrated inhibition of spirochete growth. A small pilot study in dogs indicated that two cefovecin injections two weeks apart would be as efficacious against B. burgdorferi sensu stricto as the recommended treatments using doxycycline or amoxicillin daily for 31 days. This hypothesis was tested in 17–18 week old Beagle dogs, experimentally infected with B. burgdorferisensu stricto, using wild caught ticks, 75 days prior to antimicrobial administration. Results Clinical observations for lameness were performed daily but were inconclusive as this characteristic sign of Lyme Disease rarely develops in the standard laboratory models of experimentally induced infection. However, each antibiotic tested was efficacious against B. burgdorferi as measured by a rapid elimination of spirochetes from the skin and reduced levels of circulating antibodies to B. burgdorferi. In addition, significantly less cefovecin treated animals had Lyme Disease associated histopathological changes compared to untreated dogs. Conclusions Convenia was efficacious against B. burgdorferi sensu stricto infection in dogs as determined by serological testing, PCR and histopathology results. Convenia provides an additional and effective treatment option for Lyme Disease in dogs

Modelling observable signatures of jet-ISM interaction: thermal emission and gas kinematics

Relativistic jets are believed to have a substantial impact on the gas dynamics and evolution of the interstellar medium (ISM) of their host galaxies. In this paper, we aim to draw a link between the simulations and the observable signatures of jet-ISM interactions by analyzing the emission morphology and gas kinematics resulting from jet-induced shocks in simulated disc and spherical systems. We find that the jet-induced laterally expanding forward shock of the energy bubble sweeping through the ISM causes large-scale outflows, creating shocked emission and high-velocity dispersion in the entire nuclear regions ($\sim2$ kpcs) of their hosts. The jetted systems exhibit larger velocity widths (> 800 km/s), broader Position-Velocity maps and distorted symmetry in the disc's projected velocities than systems without a jet. We also investigate the above quantities at different inclination angles of the observer with respect to the galaxy. Jets inclined to the gas disc of its host are found to be confined for longer times, and consequently couple more strongly with the disc gas. This results in prominent shocked emission and high-velocity widths, not only along the jet's path, but also in the regions perpendicular to them. Strong interaction of the jet with a gas disc can also distort its morphology. However, after the jets escape their initial confinement, the jet-disc coupling is weakened, thereby lowering the shocked emission and velocity widths.Comment: Matches the Published versio