A PILOT INVESTIGATION INTO FORCE PRODUCTION IN TETHERED SWIMMING AS AN INDIVIDUAL MONITORING TOOL

Gaining an understanding of the physical capabilities and capacities that underpin the performance of a complex skill are important to help guide performance development at the elite level. The purpose of this study was to improve understanding of the force generating characteristics that relate to performance in elite swimmers. Three elite butterfly swimmers were tested over six weeks to examine the interactions between force-derived variables from 30 s of maximal tethered swimming and maximal 50m swimming performance, both performed using the butterfly stroke. Mean force, peak force and impulse per stroke all displayed very strong relationships with 50m swim time (r \u3e -0.8) and distance per stroke (r \u3e 0.8) in group analysis. Peak force and impulse per stroke were better than mean force at discriminating variations in performance for an individual

Proteomic Analysis of Neisseria gonorrhoeae Biofilms Shows Shift to Anaerobic Respiration and Changes in Nutrient Transport and Outermembrane Proteins

Neisseria gonorrhoeae, the causative agent of gonorrhea, can form biofilms in vitro and in vivo. In biofilms, the organism is more resistant to antibiotic treatment and can serve as a reservoir for chronic infection. We have used stable isotope labeling by amino acids in cell culture (SILAC) to compare protein expression in biofilm and planktonic organisms. Two parallel populations of N. gonorrhoeae strain 1291, which is an arginine auxotroph, were grown for 48 h in continuous-flow chambers over glass, one supplemented with 13C6-arginine for planktonic organisms and the other with unlabeled arginine for biofilm growth. The biofilm and planktonic cells were harvested and lysed separately, and fractionated into three sequential protein extracts. Corresponding heavy (H) planktonic and light (L) biofilm protein extracts were mixed and separated by 1D SDS-PAGE gels, and samples were extensively analyzed by liquid chromatography-mass spectrometry. Overall, 757 proteins were identified, and 152 unique proteins met a 1.5-fold cutoff threshold for differential expression with p-values <0.05. Comparing biofilm to planktonic organisms, this set included 73 upregulated and 54 downregulated proteins. Nearly a third of the upregulated proteins were involved in energy metabolism, with cell envelope proteins making up the next largest group. Of the downregulated proteins, the largest groups were involved in protein synthesis and energy metabolism. These proteomics results were compared with our previously reported results from transcriptional profiling of gonococcal biofilms using microarrays. Nitrite reductase and cytochrome c peroxidase, key enzymes required for anaerobic growth, were detected as highly upregulated in both the proteomic and transcriptomic datasets. These and other protein expression changes observed in the present study were consistent with a shift to anaerobic respiration in gonococcal biofilms, although changes in membrane proteins not explicitly related to this shift may have other functions

Antimony Anomalies around abandoned Silver Mines in Tyrol/Austria

Austria was one of the richest Silver producers in Medieval Times, with most of the important mines located in Tyrol. Nearly all those mines in the Inntal Valley mined Fahlores of which the Silver contents were the basis for Tyrol’s political power by that time. In 1999 a large rock slide caused the last working mine to shut down, and intensive geotechnical, geological, and hydrogeological investigations started around the sliding area. These investigations found Sb anomalies in many springs around the dolomite underground mine and in the underground mine itself. While some scientists recognized a close relation to the former Silver mine, the mine owner suggested a relation to the geological situation. About 50 sampling points in different geological strata near the Großkogel and Schwaz dolomite and visitor’s mines were sampled in summer and winter and analysed for the main and trace elements. As a result of those investigations we were able to show, that the anomalies are closely linked to the geological situation and that mined as well as unmined areas are subject to Sb anomalies of up to 2.2 mg L-1. It is clear from our investigations, that the Antimony anomalies are linked to the geological situation and that the current operators are not responsible for the Antimony concentrations that can be found today

FemZone trial: a randomized phase II trial comparing neoadjuvant letrozole and zoledronic acid with letrozole in primary breast cancer patients

Background: The objective of this prospectively randomized phase II trial (Trial registration: EUCTR2004-004007-37-DE) was to compare the clinical response of primary breast cancer patients to neoadjuvant therapy with letrozole alone (LET) or letrozole and zoledronic acid (LET + ZOL). Methods: Patients were randomly assigned to receive either LET 2.5 mg/day (n = 79) or the combination of LET 2.5 mg/day and a total of seven infusions of ZOL 4 mg every 4 weeks (n = 89) for 6 months. Primary endpoint was clinical response rate as assessed by mammogram readings. The study was terminated prematurely due to insufficient recruitment. We report here on an exploratory analysis of this data. Results: Central assessment of tumor sizes during the treatment period was available for 131 patients (66 LET, 65 LET + ZOL). Clinical responses (complete or partial) were seen in 54.5% (95% CI: 41.8-66.9) of the patients in the LET arm and 69.2% (95% CI: 56.6-80.1) of those in the LET + ZOL arm (P = 0.106). A multivariate model showed an OR of 1.72 (95% CI: 0.83-3.59) for the experimental arm. Conclusion: No increase in the clinical response rate was observed with the addition of ZOL to a neoadjuvant treatment regimen with LET. However a trend towards a better reponse in the LET + ZOL arm could be observed. This trend is consistent with previous studies that have investigated the addition of ZOL to chemotherapy, and it may support the evidence for a direct antitumor action of zoledronic acid

The Dutch list of essential drugs for undergraduate medical education: A modified Delphi study

Aims: Prescribing errors among junior doctors are common in clinical practice because many lack prescribing competence after graduation. This is in part due to inadequate education in clinical pharmacology and therapeutics (CP&T) in the undergraduate medical curriculum. To support CP&T education, it is important to determine which drugs medical undergraduates should be able to prescribe safely and effectively without direct supervision by the time they graduate. Currently, there is no such list with broad-based consensus. Therefore, the aim was to reach consensus on a list of essential drugs for undergraduate medical education in the Netherlands. Methods: A two-round modified Delphi study was conducted among pharmacists, medical specialists, junior doctors and pharmacotherapy teachers from all eight Dutch academic hospitals. Participants were asked to indicate whether it was essential that medical graduates could prescribe specific drugs included on a preliminary list. Drugs for which ≥80% of all respondents agreed or strongly agreed were included in the final list. Results: In all, 42 (65%) participants completed the two Delphi rounds. A total of 132 drugs (39%) from the preliminary list and two (3%) newly proposed drugs were included. Conclusions: This is the first Delphi consensus study to identify the drugs that Dutch junior doctors should be able to prescribe safely and effectively without direct supervision. This list can be used to harmonize and support the teaching and assessment of CP&T. Moreover, this study shows that a Delphi method is suitable to reach consensus on such a list, and could be used for a European list

The Taxonomy and Ontology of Sexual Difference: Implications for Sport

When it comes to sexing the body, the effect of increasing knowledge has not been to consolidate the two-sex model of sexual difference, but to challenge the certainties of binary thinking. While this has consequences across all discourses, sport finds itself in a particularly bright spotlight because of its reliance on a clear distinction between male and female bodies. This article argues that sex testing is not based on knowledge of reality, but on an edifice of gender ideology that is simplistic and out-dated. It proposes that, in the light of recent controversies, there is now an urgent requirement to take the growing challenges to the taxonomy and ontology of sexual difference seriously. This should be done through a pro-active programme of education, targeted at all those concerned with sport, so that they can think differently rather than attempt to bolster the status quo