Discourse Dimensions Of "Go": Sounds, Gestures, and Speech

This paper examines the discourse dimensions of “go.” When used as a quotative, “go” functions in three capacities in introducing sounds, gestures, and speech. A world wide phenomenon, using “go” to introduce speech has become prominent in the last fifty years, but is understood by almost all native English speakers. Using “go” to indicate sound, gestures, and speech is certainly an informal register and is restricted to sentential con- straints of location and even tense. Examples from conversation, song lyrics, written texts, and television transcripts reveal the prominence of the discourse aspect of “go” to mark sounds, gestures and speech.Este artículo examina las dimensiones discursivas del verbo “go” inglés. Cuando se usa como cuotativo, “go” puede desempeñar distintas funciones al introducir sonidos, gestos y discurso. Como fenómeno mundial, el uso de “go” para introducir discurso ha alcanzado una mayor importancia en los últimos cincuenta años, aunque la mayoría de los hablantes nativos del inglés lo interpretan correctamente. Cabe señalar que el uso del verbo “go” para introducir sonidos, gestos y discurso se encuentra limitado a contextos de registro informal, así como a una determinada posición en la oración e incluso al tiempo verbal empleado. El análisis de ejemplos extraídos de conversaciones, letras de canciones, textos escritos y transcripciones de la televisión revelan la importancia del aspecto discursivo del verbo “go” en inglés para señalar sonidos, gestos y discurso

A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein.

Funder: G. Harold and Leila Y. Mathers Charitable Foundation; funder-id: http://dx.doi.org/10.13039/100001229Apolipoprotein B-containing lipoproteins (B-lps) are essential for the transport of hydrophobic dietary and endogenous lipids through the circulation in vertebrates. Zebrafish embryos produce large numbers of B-lps in the yolk syncytial layer (YSL) to move lipids from yolk to growing tissues. Disruptions in B-lp production perturb yolk morphology, readily allowing for visual identification of mutants with altered B-lp metabolism. Here we report the discovery of a missense mutation in microsomal triglyceride transfer protein (Mtp), a protein that is essential for B-lp production. This mutation of a conserved glycine residue to valine (zebrafish G863V, human G865V) reduces B-lp production and results in yolk opacity due to aberrant accumulation of cytoplasmic lipid droplets in the YSL. However, this phenotype is milder than that of the previously reported L475P stalactite (stl) mutation. MTP transfers lipids, including triglycerides and phospholipids, to apolipoprotein B in the ER for B-lp assembly. In vitro lipid transfer assays reveal that while both MTP mutations eliminate triglyceride transfer activity, the G863V mutant protein unexpectedly retains ~80% of phospholipid transfer activity. This residual phospholipid transfer activity of the G863V mttp mutant protein is sufficient to support the secretion of small B-lps, which prevents intestinal fat malabsorption and growth defects observed in the mttpstl/stl mutant zebrafish. Modeling based on the recent crystal structure of the heterodimeric human MTP complex suggests the G865V mutation may block triglyceride entry into the lipid-binding cavity. Together, these data argue that selective inhibition of MTP triglyceride transfer activity may be a feasible therapeutic approach to treat dyslipidemia and provide structural insight for drug design. These data also highlight the power of yolk transport studies to identify proteins critical for B-lp biology

Deconstructing HD 28867

The 3" pair of B9 stars, HD 28867, is one of the brightest X-ray sources in the Taurus-Auriga star forming region. In this multi-wavelength study, we attempt to deduce the source of the X-ray emission. We show that the East component is the X-ray source. The East component has a near-IR excess and displays narrow absorption lines in the optical, both of which are consistent with a cool stellar companion. This companion is one of the brightest low mass pre-main sequence stars in Tau-Aur; at 2 microns it and the B9 star are equally bright. We see evidence for radial velocity variability in the cool component of >34 km/s. It is not visible in K band speckle imaging, which constrains the companion to lie within 14 AU of the B star. We also report on a possible fourth member of the group, an M1 star 18" south of HD 28867.Comment: accepted by the Astronomical Journa

Early Response to Preventive Strategies in the Diabetes Prevention Program

BACKGROUND Recommendations for diabetes prevention in patients with prediabetes include lifestyle modification and metformin. However, the significance of early weight loss and glucose measurements when monitoring response to these proven interventions is unknown. OBJECTIVE To quantify the relationship between early measures of weight and glucose and subsequent diabetes in patients undergoing diabetes prevention interventions. DESIGN Analysis of results from a randomized controlled trial in 27 academic medical centers in the United States. PARTICIPANTS/INTERVENTIONS 3,041 adults with hyperglycemia randomized to lifestyle (n = 1,018), metformin (n = 1,036), or placebo (n = 987) with complete follow-up in The Diabetes Prevention Program. MAIN MEASURES Independent variables were weight loss at 6 and 12 months; fasting glucose (FG) at 6 months; hemoglobin A1c (HbA1c) at 6 months; and post-load glucose at 12 months. The main outcome was time to diabetes diagnosis. KEY RESULTS After 6 months, 604 participants developed diabetes in the lifestyle (n = 140), metformin (n = 206), and placebo (n = 258) arms over 2.7 years. In the lifestyle arm, 6-month weight loss predicted decreased diabetes risk in a graded fashion: adjusted HR (95 % CI) 0.65 (0.35–1.22), 0.62 (0.33–1.18), 0.46 (0.24–0.87), 0.34 (0.18–0.64), and 0.15 (0.07–0.30) for 0–60 % lower diabetes risk across arms. We found a significant interaction between 6-month weight loss and FG in the lifestyle arm (P = 0.038). CONCLUSION Weight and glucose at 6 and 12 months strongly predict lower subsequent diabetes risk with a lifestyle intervention; lower FG predicts lower risk even with substantial weight loss. Early reduction in glycemia is a stronger predictor of future diabetes risk than weight loss for metformin. We offer the first evidence to guide clinicians in making interval management decisions for high-risk patients undertaking measures to prevent diabetes

Mass Determination and Detection of the Onset of Chromospheric Activity for the Sub-Stellar Object in EF Eridani

EF Eri is a magnetic cataclysmic variable that has been in a low accretion state for the past nine years. Low state optical spectra reveal the underlying Zeeman-split white dwarf absorption lines. These features are used to determine a value of 13-14 MG as the white dwarf field strength. Recently, 5-7 years into the low state, Balmer and other emission lines have appeared in the optical. An analysis of the H$\alpha$ emission line yields the first radial velocity solution for EF Eri, leading to a spectroscopic ephemeris for the binary and, using the best available white dwarf mass of 0.6M${\odot}$, a mass estimate for the secondary of 0.055M${\odot}$. For a white dwarf mass of 0.95M${\odot}$, the average for magnetic white dwarfs, the secondary mass increases to 0.087M${\odot}$. At EF Eri's orbital period of 81 minutes, this higher mass secondary could not be a normal star and still fit within the Roche lobe. The source of the Balmer and other emission lines is confirmed to be from the sub-stellar secondary and we argue that it is due to stellar activity. We compare EF Eri's emission line spectrum and activity behavior to that recently observed in AM Her and VV Pup and attributed to stellar activity. We explore observations and models originally developed for V471 Tau, for the RS CVn binaries, and for extra-solar planets. We conclude that irradiation of the secondary in EF Eri and similar systems is unlikely and, in polars, the magnetic field interaction between the two stars (with a possible tidal component) is a probable mechanism which would concentrate chromospheric activity on the secondary near the sub-stellar point of the white dwarf.Comment: 49 pages, 12 figures Accepted to ApJ (Main journal

?2-Microglobulin Amyloid Fibril-Induced Membrane Disruption Is Enhanced by Endosomal Lipids and Acidic pH

Although the molecular mechanisms underlying the pathology of amyloidoses are not well understood, the interaction between amyloid proteins and cell membranes is thought to play a role in several amyloid diseases. Amyloid fibrils of ?2-microglobulin (?2m), associated with dialysis-related amyloidosis (DRA), have been shown to cause disruption of anionic lipid bilayers in vitro. However, the effect of lipid composition and the chemical environment in which ?2m-lipid interactions occur have not been investigated previously. Here we examine membrane damage resulting from the interaction of ?2m monomers and fibrils with lipid bilayers. Using dye release, tryptophan fluorescence quenching and fluorescence confocal microscopy assays we investigate the effect of anionic lipid composition and pH on the susceptibility of liposomes to fibril-induced membrane damage. We show that ?2m fibril-induced membrane disruption is modulated by anionic lipid composition and is enhanced by acidic pH. Most strikingly, the greatest degree of membrane disruption is observed for liposomes containing bis(monoacylglycero)phosphate (BMP) at acidic pH, conditions likely to reflect those encountered in the endocytic pathway. The results suggest that the interaction between ?2m fibrils and membranes of endosomal origin may play a role in the molecular mechanism of ?2m amyloid-associated osteoarticular tissue destruction in DRA

For public (and recontextualized) sociology: The promises and perils of public engagement in an age of mediated communication

This article argues for the analysis of public engagement as an essentially mediated activity. Although recent studies note that academic knowledge is increasingly available for consumption by nonacademic audiences, they tell us little about how it gets recontextualized while passing through the hands of media professionals on its way toward such audiences. In Burawoy’s (2005) influential call for the rebirth of public sociology, as in the debates his work provoked, the media is treated solely as a means for the transportation of knowledge. But as this article demonstrates, the media does not simply transport knowledge; it also, and at the same time, translates that knowledge in various, rhetorically consequential ways. Focusing on the mediated trajectory of an attempt by a group of academics to connect with audiences beyond academia, their initial contribution is compared to its subsequent translation(s) across various British newspapers. A discursive analysis reveals the techniques via which a classic form of public sociology came to be recontextualized such that, remarkably, these authors were left appearing to voice nothing but their own petty prejudices. The article concludes by noting that where public engagement involves mediation, public sociology should pay more attention to the recontextualizing affordances of media discourse