Prediction of Apple Maggot Fly Emergence from Thermal Unit Accumulation

The minimum temperature threshold for the development of apple maggot pupae, Rhagoletis pomonella was 6.4°C. From 1951-75 the 1st flies emerged in cages over infested apples on the avg on June 15 near Highland, NY and on June 23 in Geneva, NY. The avg accumulated air temperature thermal units (T.U.'s) from Mar. 1 using a threshold of 6.4°C until 1st emergence were 614±53 and 641±48, respectively, at the 2 locations. The annual deviations between the actual first emergence and the date when the avg T.U.'s were accumulated ranged from 1-8 days with an avg of 3.5 at Geneva and from 1-14 days with an avg of 5-7 days at Highland. From 1975-77 the mean T.U. accumulation and the 99% confidence interval developed at Geneva was used to predict emergence in 5 locations in Wayne Co. The deviations between the observed emergence and dates in which the appropriate number of T.U.'s was accumulated averaged 3.5 and 0.8 days, respectivel

Chem-News - An On-Line Pesticide Information Program

Computerization of pesticide information is rapidly becoming a necessity as regulatory agencies expand their activities through enforcement, monitoring, and certification of pesticide applicators. Educational institutions responsible for providing pesticide information and pesticide applicator training for certification must also expand their capabilities for immediate updating and faster retrieval. Two programs at Cornell University, the Chemical-Pesticides Program and the Pesticide Impact Assessment Program (PIAP), are presently involved in developing on-line pesticide information for researchers, extension personnel, and regulatory agencies, as well as for those using pesticides

Submerged in the mainstream? A case study of an immigrant learner in a New Zealand primary classroom

Immigrant children from diverse language backgrounds face not only linguistic challenges when enrolled in mainstream English-medium classrooms, but also difficulties adjusting to an unfamiliar learning community. The culture of primary school classrooms in New Zealand typically reflects conventions across three dimensions: interactional, instructional task performance and cognitive-academic development. All three dimensions are underpinned by the culturally specific discourse conventions involved in language socialisation. New learners may be helped by classmates or their teacher to understand and successfully use these conventions, but left on their own they may sink rather than swim. This is a case study of one Taiwanese 11-year old boy, 'John', who entered a New Zealand primary classroom midway through the school year. John's basic conversational ability was sound, but he did not possess the interactive classroom skills needed to operate in the new culture of learning. Selected from a wider study of the classroom, transcript data from audio-recorded excerpts of John's interactions over several months with his teacher and classmates are interpreted from perspectives derived from sociocultural and language socialisation theories. The article concludes with a brief consideration of the extent to which John constructed, or was constrained from constructing meaningful learning experiences, and suggestions for further research and reflection

In vivo kidney storage

A prelininary communicatio

When to stay, when to go: trade-offs for southern African arid-zone birds in times of drought

Arid environments remind one of the punctuated equilibrium theory of evolution : they experience long periods of stasis and low productivity, interrupted with episodic rainfall which spurs reproduction and movement. Birds, as highly dispersive organisms, are among the most dramatic indicators of these fluctuations. Here we review birds' two main strategies, residency and nomadism, and the trade-offs faced by individuals in uncertain times. In general, wet years stimulate higher densities of nests (i.e. smaller territories), larger clutch sizes, unseasonal breeding, and at some times of year, higher breeding success. Rainfall above a certain threshold triggers breeding in resident species and an influx of nomadic species which breed and then move on. The environmental cues which trigger nomadism are sometimes poorly understood, but include distant thunderstorms for aquatic species, and perhaps for insectivores. Environmental cues that draw nomadic granivores to areas that have had recent rain are not known

On the artificial nature of aperiodic variability in XMM-Newton observations of M 31 X-ray sources and the ultraluminous X-ray source NGC 4559 ULX-7

Context: Power density spectra (PDS) that are characteristic of low mass X-ray binaries (LMXBs) have been previously reported for M 31 X-ray sources, observed by XMM-Newton. However, we have recently discovered that these PDS result from the improper addition/subtraction of non-simultaneous lightcurves. Aims: To understand the properties and origins of the artefact. Methods: We re-analysed our XMM-Newton observations of M 31 with non-simultaneous and simultaneous lightcurves, then combined simulated lightcurves at various intensities with various offsets and found that the artefact is more dependent on the offset than the intensity. Results: The lightcurves produced by the XMM-Newton Science Analysis Software (SAS) are non-synchronised by default. This affects not only the combination of lightcurves from the three EPIC detectors (MOS1, MOS2 and pn), but also background subtraction in the same CCD. It is therefore imperative that all SAS-generated lightcurves are synchronised by time filtering, even if the whole observation is to be used. We also find that the reported timing behaviour for NGC 4559 ULX-7 was also contaminated by the artefact; there is no significant variability in the correctly-combined lightcurves of NGC 4559 ULX-7. Hence, the classification of this source as an intermediate-mass black hole is no longer justified. Conclusions: While previous timing results from M 31 have been proven wrong, and also the broken power law PDS in NGC 4559 ULX-7, XMM-Newton was able to detect aperiodic variability in just 3 ks of observations of NGC 5408 ULX1. Hence XMM-Newton remains a viable tool for analysing variability in extra-galactic X-ray sources

Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus.

Sialic acids (Sias) are important glycans displayed on the cells and tissues of many different animals and are frequent targets for binding and modification by pathogens, including influenza viruses. Influenza virus hemagglutinins bind Sias during the infection of their normal hosts, while the encoded neuraminidases and/or esterases remove or modify the Sia to allow virion release or to prevent rebinding. Sias naturally occur in a variety of modified forms, and modified Sias can alter influenza virus host tropisms through their altered interactions with the viral glycoproteins. However, the distribution of modified Sia forms and their effects on pathogen-host interactions are still poorly understood. Here we used probes developed from viral Sia-binding proteins to detect O-acetylated (4-O-acetyl, 9-O-acetyl, and 7,9-O-acetyl) Sias displayed on the tissues of some natural or experimental hosts for influenza viruses. These modified Sias showed highly variable displays between the hosts and tissues examined. The 9-O-acetyl (and 7,9-) modified Sia forms were found on cells and tissues of many hosts, including mice, humans, ferrets, guinea pigs, pigs, horses, dogs, as well as in those of ducks and embryonated chicken egg tissues and membranes, although in variable amounts. The 4-O-acetyl Sias were found in the respiratory tissues of fewer animals, being primarily displayed in the horse and guinea pig, but were not detected in humans or pigs. The results suggest that these Sia variants may influence virus tropisms by altering and selecting their cell interactions. IMPORTANCE Sialic acids (Sias) are key glycans that control or modulate many normal cell and tissue functions while also interacting with a variety of pathogens, including many different viruses. Sias are naturally displayed in a variety of different forms, with modifications at several positions that can alter their functional interactions with pathogens. In addition, Sias are often modified or removed by enzymes such as host or pathogen esterases or sialidases (neuraminidases), and Sia modifications can alter those enzymatic activities to impact pathogen infections. Sia chemical diversity in different hosts and tissues likely alters the pathogen-host interactions and influences the outcome of infection. Here we explored the display of 4-O-acetyl, 9-O-acetyl, and 7,9-O-acetyl modified Sia forms in some target tissues for influenza virus infection in mice, humans, birds, guinea pigs, ferrets, swine, horses, and dogs, which encompass many natural and laboratory hosts of those viruses

SCUBA observations of the host galaxies of four dark gamma-ray bursts

We present the results of a search for submillimetre-luminous host galaxies of optically dark gamma-ray bursts (GRBs) using the Submillimetre Common-User Bolometer Array (SCUBA) on the James Clerk Maxwell Telescope (JCMT). We made photometry measurements of the 850-micron flux at the location of four `dark bursts', which are those with no detected optical afterglow despite rapid deep searches, and which may therefore be within galaxies containing substantial amounts of dust. We were unable to detect any individual source significantly. Our results are consistent with predictions for the host galaxy population as a whole, rather than for a subset of dusty hosts. This indicates that optically dark GRBs are not especially associated with very submillimetre-luminous galaxies and so cannot be used as reliable indicators of dust-enshrouded massive star-formation activity. Further observations are required to establish the relationship between the wider GRB host galaxy population and SCUBA galaxies.Comment: 6 pages. Accepted for publication in MNRA

Groundwater flow code verification ``benchmarking`` activity (COVE-2A): Analysis of participants` work

The Nuclear Waste Repository Technology Department at Sandia National Laboratories (SNL) is investigating the suitability of Yucca Mountain as a potential site for underground burial of nuclear wastes. One element of the investigations is to assess the potential long-term effects of groundwater flow on the integrity of a potential repository. A number of computer codes are being used to model groundwater flow through geologic media in which the potential repository would be located. These codes compute numerical solutions for problems that are usually analytically intractable. Consequently, independent confirmation of the correctness of the solution is often not possible. Code verification is a process that permits the determination of the numerical accuracy of codes by comparing the results of several numerical solutions for the same problem. The international nuclear waste research community uses benchmarking for intercomparisons that partially satisfy the Nuclear Regulatory Commission (NRC) definition of code verification. This report presents the results from the COVE-2A (Code Verification) project, which is a subset of the COVE project

Protease inhibitors targeting coronavirus and filovirus entry.

In order to gain entry into cells, diverse viruses, including Ebola virus, SARS-coronavirus and the emerging MERS-coronavirus, depend on activation of their envelope glycoproteins by host cell proteases. The respective enzymes are thus excellent targets for antiviral intervention. In cell culture, activation of Ebola virus, as well as SARS- and MERS-coronavirus can be accomplished by the endosomal cysteine proteases, cathepsin L (CTSL) and cathepsin B (CTSB). In addition, SARS- and MERS-coronavirus can use serine proteases localized at the cell surface, for their activation. However, it is currently unclear which protease(s) facilitate viral spread in the infected host. We report here that the cysteine protease inhibitor K11777, ((2S)-N-[(1E,3S)-1-(benzenesulfonyl)-5-phenylpent-1-en-3-yl]-2-{[(E)-4-methylpiperazine-1-carbonyl]amino}-3-phenylpropanamide) and closely-related vinylsulfones act as broad-spectrum antivirals by targeting cathepsin-mediated cell entry. K11777 is already in advanced stages of development for a number of parasitic diseases, such as Chagas disease, and has proven to be safe and effective in a range of animal models. K11777 inhibition of SARS-CoV and Ebola virus entry was observed in the sub-nanomolar range. In order to assess whether cysteine or serine proteases promote viral spread in the host, we compared the antiviral activity of an optimized K11777-derivative with that of camostat, an inhibitor of TMPRSS2 and related serine proteases. Employing a pathogenic animal model of SARS-CoV infection, we demonstrated that viral spread and pathogenesis of SARS-CoV is driven by serine rather than cysteine proteases and can be effectively prevented by camostat. Camostat has been clinically used to treat chronic pancreatitis, and thus represents an exciting potential therapeutic for respiratory coronavirus infections. Our results indicate that camostat, or similar serine protease inhibitors, might be an effective option for treatment of SARS and potentially MERS, while vinyl sulfone-based inhibitors are excellent lead candidates for Ebola virus therapeutics