Fatigue in seafarers working in the offshore oil and gas re-supply industry: effects of safety climate, psychosocial work environment and shift arrangement

This study examined the influence of safety climate and psychosocial work environment on the reportedfatigue of seafarers working in the offshore oil and gas re-supply industry (n = 402). We found that seafarerswho reported high psychological demands and perceived the organisational-level safety climate negatively,reported significantly more mental fatigue, physical fatigue, and lack of energy. In addition, seafarers whoreported having high levels of job control reported being significantly less mentally fatigued. We also foundsome combined effects of safety climate and shift arrangement. Organisational-level safety climate did notinfluence the levels of physical fatigue in seafarers working on the night shift. On the contrary, seafarersworking during the days reported to be more physically fatigued when they perceived the organisational-levelclimate to be negative compared with the positive. The opposite effect was found for group-level safetyclimate: seafarers working during the nights reported to be more physically fatigued when they perceivedthe group-level climate to be negative compared with the positive. The results from this study point to theimportance of taking into consideration aspects of the psychosocial work environment and safety climate,and their potential impact on fatigue and safety in the maritime organisations

Chromosome-level assemblies from diverse clades reveal limited structural and gene content variation in the genome of Candida glabrata

Background Candida glabrata is an opportunistic yeast pathogen thought to have a large genetic and phenotypic diversity and a highly plastic genome. However, the lack of chromosome-level genome assemblies representing this diversity limits our ability to accurately establish how chromosomal structure and gene content vary across strains. Results Here, we expanded publicly available assemblies by using long-read sequencing technologies in twelve diverse strains, obtaining a final set of twenty-one chromosome-level genomes spanning the known C. glabrata diversity. Using comparative approaches, we inferred variation in chromosome structure and determined the pan-genome, including an analysis of the adhesin gene repertoire. Our analysis uncovered four new adhesin orthogroups and inferred a rich ancestral adhesion repertoire, which was subsequently shaped through a still ongoing process of gene loss, gene duplication, and gene conversion. Conclusions C. glabrata has a largely stable pan-genome except for a highly variable subset of genes encoding cell wall-associated functions. Adhesin repertoire was established for each strain and showed variability among clades.TG group acknowledges support from the Spanish Ministry of Science and Innovation (MCIN) for grant PGC2018-099921-B-I00, cofounded by European Regional Development Fund (ERDF); from the Catalan Research Agency (AGAUR) SGR423; from the European Union’s Horizon 2020 research and innovation programme (ERC-2016-724173); from the Gordon and Betty Moore Foundation (Grant GBMF9742); from the “La Caixa” foundation (Grant LCF/PR/HR21/00737), and from the Instituto de Salud Carlos III (IMPACT Grant IMP/00019 and CIBERINFEC CB21/13/00061- ISCIII-SGEFI/ERDF). PWJG acknowledges support by grants SBPLY/19/180501/000114 and SBPLY/19/180501/000356 funded by the Regional government of Castilla-La Mancha and grants SAF2013-47570-P and PID2020-117983RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by ERDF a way of making Europe.Peer ReviewedPostprint (author's final draft

Jove i participatiu : requisits del nou model de transmissió cultural

La imitació com a mètode d'aprenentatge humà mai ha estat un procés simple. L'evolució cultural de l'espècie humana que d'ella es deriva segueix arrelada a una actitud selectiva -els models escollits es caracteritzen per ser individus amb prestigi-, però sembla ser que aquesta reputació ja no recau en els mateixos de sempre. Mitjançant un estudi de camp amb els Tsimane' -un grup ètnic bolivià-, s'ha vist que el lideratge ha passat a mans dels més joves, tenint aquests un alt nivell educatiu i una forta vinculació amb el treball comunitari.La imitación como método de aprendizaje humano nunca ha sido un proceso simple. La evolución cultural de la especie humana que de ella se deriva sigue arraigada a una actitud selectiva -los modelos escogidos se caracterizan por ser individuos con prestigio-, pero parece ser que esa reputación ya no recae en los mismos de siempre. Mediante un estudio de campo con los Tsimane -un grupo étnico boliviano-, se ha visto que el liderazgo ha pasado a manos de los más jóvenes, teniendo éstos un alto nivel educativo y una fuerte vinculación con el trabajo comunitario.Imitation as a method of human learning has never been a simple process. The cultural evolution of the human species based on this method continues to be established in a selective attitude - the selected models are characterized for being prestigious individuals. However, it seems to be that this prestige is no longer conferred to the same models as before. A field study with the Tsimane' - an ethnic group in Bolivia-, has demonstrated that the leadership has moved to the hands of the youngest, who have a high educational level and strong links with community work

Reprogramming human T cell function and specificity with non-viral genome targeting.

Decades of work have aimed to genetically reprogram T cells for therapeutic purposes1,2 using recombinant viral vectors, which do not target transgenes to specific genomic sites3,4. The need for viral vectors has slowed down research and clinical use as their manufacturing and testing is lengthy and expensive. Genome editing brought the promise of specific and efficient insertion of large transgenes into target cells using homology-directed repair5,6. Here we developed a CRISPR-Cas9 genome-targeting system that does not require viral vectors, allowing rapid and efficient insertion of large DNA sequences (greater than one kilobase) at specific sites in the genomes of primary human T cells, while preserving cell viability and function. This permits individual or multiplexed modification of endogenous genes. First, we applied this strategy to correct a pathogenic IL2RA mutation in cells from patients with monogenic autoimmune disease, and demonstrate improved signalling function. Second, we replaced the endogenous T cell receptor (TCR) locus with a new TCR that redirected T cells to a cancer antigen. The resulting TCR-engineered T cells specifically recognized tumour antigens and mounted productive anti-tumour cell responses in vitro and in vivo. Together, these studies provide preclinical evidence that non-viral genome targeting can enable rapid and flexible experimental manipulation and therapeutic engineering of primary human immune cells

The association of HLA-DQB1, -DQA1 and -DPB1 alleles with anti- glomerular basement membrane (GBM) disease in Chinese patients

<p>Abstract</p> <p>Background</p> <p>Human leukocyte antigen (HLA) alleles are associated with many autoimmune diseases, including anti-glomerular basement membrane (GBM) disease. In our previous study, it was demonstrated that HLA-DRB1*1501 was strongly associated with anti-GBM disease in Chinese. However, the association of anti-GBM disease and other HLA class II genes, including HLA-DQB1, -DQA1,-DPB1 alleles, has rarely been investigated in Asian, especially Chinese patients. The present study further analyzed the association between anti-GBM disease and HLA-DQB1, -DQA1, and -DPB1 genes. Apart from this, we tried to locate the potential risk amino acid residues of anti-GBM disease.</p> <p>Methods</p> <p>This study included 44 Chinese patients with anti-GBM disease and 200 healthy controls. The clinical and pathological data of the patients were collected and analyzed. Typing of HLA-DQB1, -DQA1 and -DPB1 alleles were performed by bi-directional sequencing of exon 2 using the SeCoreTM Sequencing Kits.</p> <p>Results</p> <p>Compared with normal controls, the prevalence of HLA-DPB1*0401 was significantly lower in patients with anti-GBM disease (3/88 vs. 74/400, p = 4.4 × 10^-4, pc = 0.039). Comparing with normal controls, the combination of presence of DRB1*1501 and absence of DPB1*0401 was significantly prominent among anti-GBM patients (p = 2.0 × 10^-12, pc = 1.7 × 10^-10).</p> <p>Conclusions</p> <p>HLA-DPB1*0401 might be a protective allele to anti-GBM disease in Chinese patients. The combined presence of DRB1*1501 and absence of DPB1*0401 might have an even higher risk to anti-GBM disease than HLA-DRB1*1501 alone.</p

Basement membrane components are key players in specialized extracellular matrices

More than three decades ago, basement membranes (BMs) were described as membrane-like structures capable of isolating a cell from and connecting a cell to its environment. Since this time, it has been revealed that BMs are specialized extracellular matrices (sECMs) with unique components that support important functions including differentiation, proliferation, migration, and chemotaxis of cells during development. The composition of these sECM is as unique as the tissues to which they are localized, opening the possibility that such matrices can fulfill distinct functions. Changes in BM composition play significant roles in facilitating the development of various diseases. Furthermore, tissues have to provide sECM for their stem cells during development and for their adult life. Here, we briefly review the latest research on these unique sECM and their components with a special emphasis on embryonic and adult stem cells and their niches