Detection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapy

WOS: 000445944700005PubMed: 30041285Canine transmissible venereal tumor (CTVT) is a tumor that commonly occurs in genital and extragenital sites of both genders. Long interspersed nuclear elements (LINE-1) retrotransposon has a pivotal role in allogenic transfection among uncontrolled dog populations. This study aimed to perform pathomorphological, immunohistochemical, and in situ polymerase chain reaction (PCR) evaluation of CTVT (n = 18) in transfected dogs during chemotherapy. Immunohistochemically, tumor phases were investigated by using specific markers (CD3, CD4, CD8, CD79, and transforming growth factor beta [TGF-beta]), and investigated an amplified specific sequence of TVT LINE-1 retrotransposon by in situ PCR. Polyhedral-shaped neoplastic cells that had large, round, hypo/hyperchromatic nuclei and eosinophilic cytoplasm were detected. All marker results were positive, especially in the early weeks of recovery. CD4 and TGF-beta markers were conspicuously positive at the initial stage. In situ PCR LINE-1 sequence was initially positive in only four cases. It is believed that the CD and TGF-beta markers provide phase identification at tumor initiation and during chemotherapy. It is thought that presence of T and B lymphocytes, which have roles in cellular and humoral immunity, is needed so that regression of the tumor is possible.Ankara University Scientific Research Projects, Turkey [10B3338008/2014]This study was supported by the Ankara University Scientific Research Projects (project No. 10B3338008/2014), Turkey

Evalution of the relationship between milk glutathione peroxidase activity, milk composition and various parameters of subclinical mastitis under seasonal variations

YAZLIK, Murat Onur/0000-0002-0039-5597WOS: 000411813300003The aims of this study were to evaluate the Glutathione peroxidase (GSH-Px) activity in milk, and various milk parameters [milk fat, milk protein, milk solids non-fat (SNF), somatic cell count (SCC), total bacteria count (TBC), electrical conductivity (EC)] in healthy dairy cows during lactation, and to demonstrate the significant correlations between GSH-Px activity, milk composition, SCC, TBC and EC under seasonal variations. The cows calving in January were assigned into the winter group (n = 30) while the other cows calving in August were assigned into the summer group (n = 30). Milk samples were collected weekly during lactation, and monthly average values of the parameters were determined. Although milk GSH-Px activity was variable during lactation, it was significantly higher in the winter group compared to the summer group of cows during the first five months of lactation (P<0.001). However, between the 6th and 10th months of lactation, GSH-Px activity was significantly higher in the summer group (P<0.001). In both groups of cows, GSH-Px activity was negatively correlated with SCC, TBC and EC during lactation. No correlation was found between GSH-activity and milk composition. In conclusion, the alteration of milk GSH-Px levels could be used as a diagnostic tool for monitoring udder health due to its relationship with the parameters of subclinical mastitis. Furthermore, cows calving in winter had higher GSH-Px activity than those calving in summer, especially during the first few months of lactation. These findings indicate that milk GSH-Px activity in cows during lactation could be influenced by seasonal variations

Evaluation of the relationship between milk glutathione peroxidase activity, milk composition and various parameters of subclinical mastitis under seasonal variations

The aims of this study were to evaluate the Glutathione peroxidase (GSH-Px) activity in milk, and various milk parameters [milk fat, milk protein, milk solids non-fat (SNF), somatic cell count (SCC), total bacteria count (TBC), electrical conductivity (EC)] in healthy dairy cows during lactation, and to demonstrate the significant correlations between GSH-Px activity, milk composition, SCC, TBC and EC under seasonal variations. The cows calving in January were assigned into the winter group (n = 30) while the other cows calving in August were assigned into the summer group (n = 30). Milk samples were collected weekly during lactation, and monthly average values of the parameters were determined. Although milk GSH-Px activity was variable during lactation, it was significantly higher in the winter group compared to the summer group of cows during the first five months of lactation (P<0.001). However, between the 6th and 10th months of lactation, GSH-Px activity was significantly higher in the summer group (P<0.001). In both groups of cows, GSH-Px activity was negatively correlated with SCC, TBC and EC during lactation. No correlation was found between GSH-Px activity and milk composition. In conclusion, the alteration of milk GSH-Px levels could be used as a diagnostic tool for monitoring udder health due to its relationship with the parameters of subclinical mastitis. Furthermore, cows calving in winter had higher GSH-Px activity than those calving in summer, especially during the first few months of lactation. These findings indicate that milk GSH-Px activity in cows during lactation could be influenced by seasonal variations.Ciljevi ovog istraživanja bili su, tijekom laktacje zdravih krava, procijeniti aktivnost glutation peroksidaze (GSH-Px) u mlijeku te odrediti različite druge sastojke (mliječnu masti, mliječne bjelančevine, bezmasnu suhu tvar, broj somatskih stanica (SCC), ukupan broj bakterija (TBC), električnu provodljivost (EC) i prikazati značajnost korelacija između aktivnosti GSH-Px, sastava mlijeka, SCC, TBC i EC u uvjetima različitih sezona. Krave oteljene u siječnju raspoređene su u zimsku skupinu (n = 30), dok su ostale krave oteljene u kolovozu dodijeljene ljetnoj skupini (n = 30). Tijekom laktacije, uzorci mlijeka su prikupljani jednom tjedno i na temelju toga za svaki pokazatelj utvrđene su prosječne mjesečne vrijednosti. Iako je aktivnost GSH-Px u mlijeku tijekom laktacije bila varijabilna, krave iz zimske skupine imale su u prvih pet mjesci značajno (P<0,001) višu aktivnost GSH-Px u odnosu na krave iz ljetne skupine. Međutim, između 6. i 10. mjeseca laktacije, aktivnost GSH-Px bila je znatno viša u ljetnoj skupini krava (P<0,001). U obje skupine krava aktivnost GSH-Px bila je negativno povezana sa SCC, TBC i EC tijekom laktacije. Nije pronađena korelacija između aktivnosti GSH-Px i sastava mlijeka. Zaključno, na temelju njezinog odnosa s pokazateljima supkliničkog mastitisa, promjenjivost razine GSH-Px u mlijeku mogla bi se koristiti kao dijagnostički alat u praćenju zdravlja vimena. Nadalje, krave oteljene zimi imale su veću aktivnost GSH-Px od onih oteljenih ljeti, posebno tijekom prvih nekoliko mjeseci laktacije. Ovi rezultati upućuju na to da bi aktivnost GSH-Px u mlijeku krava tijekom laktacije mogla biti pod utjecajem sezonskih promjena

The evaluation of ozone and betahistine in the treatment of tinnitus

The aim of the study is to evaluate the effectiveness of ozone and betahistine treatments in the treatment of tinnitus. Sixty-eight patients were enrolled in this randomized, prospective controlled study. The ozone group consisted of 27, betahistine group consisted of 26 and control group consisted of 15 patients. The patients in ozone group received 10 sessions of ozone treatment via major autohemotherapy. Betahistine group received 48 mg/day betahistine tablets per oral for 3 months duration. The control group was followed up without any treatment given. The evaluation of tinnitus was made by tinnitus loudness and tinnitus handicap inventory (THI). The changes in findings from baseline to 3rd and 6th months were assessed, and the group results were compared. Comparison of the initial mean tinnitus loudness and 3 and 6 months after treatment in each of the three groups did not reveal a significant difference. The comparison between the groups in terms of the improvement of tinnitus loudness was not significant (p = 0.821). Comparison of the initial mean THI and 3 and 6 months after treatment revealed a significant difference in ozone and betahistine groups but not in the control group. When the delta (Delta) THI (the change of mean THI between the initial and 6th month) was compared between the groups, there was no significant difference. This randomized controlled study investigating the effects of ozone in tinnitus tries to shed light to a new method of treatment in tinnitus. The findings of the study does not provide enough evidence to support ozone and betahistine as a treatment for tinnitus and further research on the subject is necessary