Nitric oxide binds to the proximal heme coordination site of the ferrocytochrome c/cardiolipin complex: formation mechanism and dynamics.

Mammalian mitochondrial cytochrome c interacts with cardiolipin to form a complex (cyt. c/CL) important in apoptosis. Here we show that this interaction leads to structural changes in ferrocytochrome c that leads to an open coordinate site on the central iron, resulting from the dissociation of the intrinsic methionine residue, where NO can rapidly bind (k = 1.2 x 10(7) m(-1) s(-1)). Accompanying NO binding, the proximal histidine dissociates leaving the heme pentacoordinate, in contrast to the hexacoordinate nitrosyl adducts of native ferrocytochrome c or of the protein in which the coordinating methionine is removed by chemical modification or mutation. We present the results of stopped-flow and photolysis experiments that show that following initial NO binding to the heme, there ensues an unusually complex set of kinetic steps. The spectral changes associated with these kinetic transitions, together with their dependence on NO concentration, have been determined and lead us to conclude that NO binding to cyt. c/CL takes place via an overall scheme comparable to that described for cytochrome c' and guanylate cyclase, the final product being one in which NO resides on the proximal side of the heme. In addition, novel features not observed before in other heme proteins forming pentacoordinate nitrosyl species, include a high yield of NO escape after dissociation, rapid (<1 ms) dissociation of proximal histidine upon NO binding and its very fast binding (60 ps) after NO dissociation, and the formation of a hexacoordinate intermediate. These features all point at a remarkable mobility of the proximal heme environment induced by cardiolipin

Optimum estimate of delays and dispersive effects in low-frequency interferometric observations

Modern radio interferometers sensitive to low frequencies will make use of wide-band detectors. For such wide bandwidths, dispersive atmospheric effects introduce variations in the fringe delay which change through the band of the receivers. These undesired dispersive effects must be estimated and calibrated with the highest precision. We studied the achievable precision in the estimate of the ionospheric dispersion and the dynamic range of the correlated fringes for different distributions of sub-bands in low-frequency and wide-band interferometric observations. Our study is focused on the case of sub-bands with a bandwidth much narrower than that of the total covered spectrum (case of LOFAR). We computed the uncertainty of the ionospheric delay, the delay ambiguity, and the dynamic range of the fringes using four different kinds of sub-band distributions: constant spacing between sub-bands, random spacings, spacings based on a power-law distribution, and spacings based on Golomb rulers (sets of integers whose sets of differences have non-repeated elements). For a large number of sub-bands ($> 20$, depending on the delay window) spacings based on Golomb rulers give the most precise estimates of dispersive effects and the highest fringe dynamic ranges. Spacings based on the power-law distribution give similar results, although better than those with the Golomb rulers for smaller number of sub-bands. Random distributions result in large fringe dynamic ranges, but the estimate of dispersive effects is worse. A constant spacing of sub-bands results in very bad fringe dynamic ranges, but good estimates of ionospheric dispersion. Combining all the results, the power-law distribution gives the best compromise between homogeneity in the bandwidth sampling, precision in the estimate of ionospheric effects, dynamic range of the correlated fringes, and group-delay ambiguity.Comment: 8 pages, 7 figures. Accepted for publication in A&

An Appraisal of Humanitarian Intervention under International Law

Humanitarian intervention is a controversial concept in international law. It is not provided for by the United Nations Charter as it neither constitutes a legitimate use of force authorized by the United Nations Security Council (UNSC) nor the use of force in self-defence. Article 2(4) of the United Nations Charter clearly prohibits the threat or use of force against the territorial integrity or political independence of any state1. However, since the creation of the United Nations Organization in 1945, there have been many instances of intervention on humanitarian grounds and the practice has vigorously resurged after the cold war2. In this article, we shall consider the meaning of humanitarian intervention as well as the legal basis of the doctrine. We shall also review some cases of humanitarian intervention so as to ascertain whether or not there is presently a right to humanitarian intervention in international law and finally make suggestion for the future of the doctrine

Physical limitations to the spatial resolution of solid-state detectors

In this paper we explore the effect of $\delta$-ray emission, fluctuations in th e signal deposition on the detection of charged particles in silicon-based detec tors. We show that these two effects ultimately limit the resolution that can be achieved by interpolation of the signal in finely segmented position-sensitive solid-state devices.Comment: 5 page

The association between parenting behavior and somatization in adolescents explained by physiological responses in adolescents

Introduction: This study adds to the knowledge on somatization in adolescents by exploring its relation with parenting behavior and the mediating/moderating role of physiological responses in adolescents to parenting behavior. Method: Eighteen adolescents with high and 18 adolescents with low somatization scores and their mothers completed a discussion task, from which observed parenting behavior scores were derived. Skin conductance in adolescents was measured before and during the discussion. Results: For adolescents with high levels of physiological responses, unadaptive parenting was related to a higher chance of high somatization scores. For low physiologically responsive adolescents, the relation between parenting behavior and somatization was not significant. Conclusion: Parenting behavior is not univocally related to somatization in adolescents, but the association depends on physiological responses in adolescents. (C) 2014 Elsevier B.V. All rights reserved

An eccentric companion at the edge of the brown dwarf desert orbiting the 2.4 Msun giant star HIP67537

We report the discovery of a substellar companion around the giant star HIP67537. Based on precision radial velocity measurements from CHIRON and FEROS high-resolution spectroscopic data, we derived the following orbital elements for HIP67537$\,b$: m$_b$sin$i$ = 11.1$^{+0.4}_{-1.1}$ M$_{\rm {\tiny jup}}$, $a$ = 4.9$^{+0.14}_{-0.13}$ AU and $e$ = 0.59$^{+0.05}_{-0.02}$. Considering random inclination angles, this object has $\gtrsim$ 65% probability to be above the theoretical deuterium-burning limit, thus it is one of the few known objects in the planet to brown-dwarf transition region. In addition, we analyzed the Hipparcos astrometric data of this star, from which we derived a minimum inclination angle for the companion of $\sim$ 2 deg. This value corresponds to an upper mass limit of $\sim$ 0.3 M$_\odot$, therefore the probability that HIP67537$\,b$ is stellar in nature is $\lesssim$ 7%. The large mass of the host star and the high orbital eccentricity makes HIP67537$\,b$ a very interesting and rare substellar object. This is the second candidate companion in the brown dwarf desert detected in the sample of intermediate-mass stars targeted by the EXPRESS radial velocity program, which corresponds to a detection fraction of $f$ = 1.6$^{+2.0}_{-0.5}$%. This value is larger than the fraction observed in solar-type stars, providing new observational evidence of an enhanced formation efficiency of massive substellar companions in massive disks. Finally, we speculate about different formation channels for this object.Comment: Accepted for publication to A&

Building a Socio-technical Perspective of Community Resilience with a Semiotic Approach

Situated in the diversity and adversity of real-life contexts facing crisis situations, this research aims at boosting the resilience process within communities supported by digital and social technology. In this paper, eight community leaders in different parts of the world are invited to express their issues and wishes regarding the support of technology to face social challenges. Methods and artefacts based on the Organisational Semiotics (OS) and the Socially-Aware computing have been applied to analyse and consolidate this data. By providing both a systemic view of the problem and also leading to the identification of requirements, the analysis evidences some benefits of the OS-based approach to consolidate perspectives from different real-life scenarios towards building a socio-technical solution