Supplemental materials for Largemouth Bass respirometry research: data archived in support of open-access publication

Fisheries induced evolution (FIE) is hypothesized to cause changes in the life history, physiology and behavior of exploited fish stocks. Most studies evaluating FIE have occurred in exploited marine stocks which offer little opportunity for replication and few unexploited stocks exist for comparison. Inland recreational fisheries, however, offer the opportunity to replicate experiments in multiple systems, and while rare, unexploited populations exist for comparison. We measured the resting metabolic rate of four populations of largemouth bass, two exploited and two unexploited, to evaluate whether patterns of resting metabolic rates conformed to expectations for fisheries induced evolution. This document contains the resting metabolic rate data collected in the field from September 16th 2013 through October 22nd 2013. These data form the basis for an open access publication and are archived here as supplemental information

Camera trap arrays improve detection probability of wildlife: Investigating study design considerations using an empirical dataset.

Camera trapping is a standard tool in ecological research and wildlife conservation. Study designs, particularly for small-bodied or cryptic wildlife species often attempt to boost low detection probabilities by using non-random camera placement or baited cameras, which may bias data, or incorrectly estimate detection and occupancy. We investigated the ability of non-baited, multi-camera arrays to increase detection probabilities of wildlife. Study design components were evaluated for their influence on wildlife detectability by iteratively parsing an empirical dataset (1) by different sizes of camera arrays deployed (1-10 cameras), and (2) by total season length (1-365 days). Four species from our dataset that represented a range of body sizes and differing degrees of presumed detectability based on life history traits were investigated: white-tailed deer (Odocoileus virginianus), bobcat (Lynx rufus), raccoon (Procyon lotor), and Virginia opossum (Didelphis virginiana). For all species, increasing from a single camera to a multi-camera array significantly improved detection probability across the range of season lengths and number of study sites evaluated. The use of a two camera array increased survey detection an average of 80% (range 40-128%) from the detection probability of a single camera across the four species. Species that were detected infrequently benefited most from a multiple-camera array, where the addition of up to eight cameras produced significant increases in detectability. However, for species detected at high frequencies, single cameras produced a season-long (i.e, the length of time over which cameras are deployed and actively monitored) detectability greater than 0.75. These results highlight the need for researchers to be critical about camera trap study designs based on their intended target species, as detectability for each focal species responded differently to array size and season length. We suggest that researchers a priori identify target species for which inference will be made, and then design camera trapping studies around the most difficult to detect of those species

Estimating size-specific brook trout abundance in continuously sampled headwater streams using Bayesian mixed models with zero inflation and overdispersion

Abstract -We examined habitat factors related to reach-scale brook trout Salvelinus fontinalis counts of four size classes in two headwater stream networks within two contrasting summers in Connecticut, USA. Two study stream networks (7.7 and 4.4 km) were surveyed in a spatially continuous manner in their entirety, and a set of Bayesian generalised linear mixed models was compared. Trout abundance was best described by a zero-inflated overdispersed Poisson model. The effect of habitat covariates was not always consistent among size classes and years. There were nonlinear relationships between trout counts and stream temperature in both years. Colder reaches harboured higher trout counts in the warmer summer of 2008, but this pattern was not observed in the cooler and very wet summer of 2009. Amount of pool habitat was nearly consistently important across size classes and years, and counts of the largest size class were correlated positively with maximum depth and negatively with stream gradient. Spatial mapping of trout distributions showed that reaches with high trout counts may differ among size classes, particularly between the smallest and largest size classes, suggesting that movement may allow the largest trout to exploit spatially patchy habitats in these small headwaters

Influence of rare species on electrofishing distance when estimating species richness of stream and river reaches

Abstract.-The electrofishing distance needed to estimate fish species richness at the stream or river reach scale is an important question in fisheries science. This distance is governed by the shape of the species accumulation curve, which, in turn, is influenced by a combination of factors, including the number of species, their overall abundances, habitat associations, the efficiency of the sampling method, and the occurrence of rare species. In this study we document the influence of rare species on the species accumulation curves from stream and river sites in data sets from five dispersed regions of the USA. Spatial discontinuity (i.e., a noncontinuous distribution within reaches) was observed in four of the five data sets, and the four data sets contained numerically rare species represented by one or two individuals (termed singletons and doubletons, respectively). Numerically rare species were typically proportionately rare (i.e., ,1% of the total number of individuals captured), but proportionately rare species were not always numerically rare and were dependent on the total number of fish captured. Species richness asymptotes were reached at shorter electrofishing distances when singletons and doubletons were removed. The number of singletons and doubletons in the samples remained relatively constant with increasing sampling effort (i.e., sampling distance and total abundance). Simulation modeling indicated that individual aggregation within species was not a plausible reason for spatially discontinuous species distributions. When accurately detecting the presence of species is a sampling goal, the presence and prevalence of numerically rare species may need to be considered in determining sampling protocols

Distribution of Channel Catfish Life Stages in a Prairie River Basin

To describe the pattern of use by age 0, juvenile, and adult channel catfish (Ictalurus punctatus) in a prairie river basin, we collected concurrent samples from tributaries and mainstem study sites in the Grand River basin of northern Missouri. Using standardized methods, we made collections in June, August, and October of 1997 and 1998. Age 0 channel catfish were found in all three streams in August, but rarely were observed in the tributaries during October. Juveniles (ages 1-3) and adults were observed in all three streams in all three months sampled. Tag returns suggested that age 2 and over channel catfish moved to overwintering locations in deepwater habitats in the mainstem river

Looking Ahead to Spring\u27s Returning Bounty: Natal Homing

Natal homing plays a part in fisheries restoration. This article describes research by Dr. Jason Vokoun and his students on otoliths in migratory finfish such as river herring, alewives, etc

Looking Ahead to Spring\u27s Returning Bounty: Natal Homing

Natal homing plays a part in fisheries restoration. This article describes research by Dr. Jason Vokoun and his students on otoliths in migratory finfish such as river herring, alewives, etc

Data from: Fine-scale population structure and riverscape genetics of brook trout (Salvelinus fontinalis) distributed continuously along headwater channel networks

Linear and heterogeneous habitat makes headwater stream networks an ideal ecosystem in which to test the influence of environmental factors on spatial genetic patterns of obligatory aquatic species. We investigated fine-scale population structure and influence of stream habitat on individual-level genetic differentiation in brook trout (Salvelinus fontinalis) by genotyping eight microsatellite loci in 740 individuals in two headwater channel networks (7.7 km and 4.4 km) in Connecticut, USA. A weak but statistically significant isolation-by-distance pattern was ubiquitous in both sites. In the field, many tagged individuals were recaptured in the same 50m-reaches within a single field season (summer to fall). One study site was characterized with a hierarchical population structure, where seasonal barriers (natural falls > 1.5 m in height) greatly reduced gene flow and weaker spatial patterns emerged due to the presence of tributaries, each with a group of genetically distinguishable individuals. Genetic differentiation increased when pairs of individuals were separated by high stream gradient (steep channel slope) or warm stream temperature in this site, although the evidence of their influence was equivocal. In a second site, evidence for genetic clusters was very weak at the most, but genetic differentiation between individuals was positively correlated with number of tributary confluences. We concluded that the movement of brook trout was limited in the study headwater streams, resulting in the fine-scale population structure (genetic clusters and clines) even at distances of a few kilometers, and gene flow was mitigated by “riverscape” variables, particularly by physical barriers, waterway distance (i.e., isolation-by-distance) and the presence of tributaries