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Model Scale Investigation of Blade Root Cavitation Erosion on a Set of Marine Propellers

The present study is focused on the experimental analysis of the erosion caused by cavitation occurring at the blade root for a set of three model scale marine propellers. The experimental method is based on the adoption of soft paint technique together with cavitation observations. Cavitation dynamics and erosion damage patterns are recorded using three standard cameras and one high speed camera. Standard cameras are fixed on the top of test section to continuously monitor the effect of erosion damage on the blade root, the high-speed camera has been placed at different positions to investigate detailed evolution and collapse of bubbles on pressure and suction side of propeller blades. The soft paint test damage patterns have been simultaneously analysed with the high- speed videos, and results showed remarkable agreement between the occurrence of damage on the blades and the bubble collapse of cavitation. The damage pattern and cavities collapse seem to be inversely related with the inception time of damage

Propeller geometry optimization for pressure pulses reduction: an analysis of the influence of the rake distribution

The evaluation of pressure pulses is a current issue for any high-performance propeller design. It has been addressed experimentally, by means of model tests, and numerically but in most cases the analysis has been limited to the verification of a given geometry (or, at least of few configurations) identified at the end of a traditional design loop. A more direct inclusion of pressure pulses evaluation in the design procedure, for instance by very attractive multi-objective optimization approaches, could be beneficial, especially if more accurate codes may be exploited. Among the others, BEM represent an acceptable compromise between computational costs and accuracy with the further advantage, with respect to lower fidelity approaches, to account for effects of geometrical haracteristics (such as rake distribution) which are often defined only according to designer experience and special needs. However, if the ability of the BEM methods to predict propeller performance and cavitation extension is well documented, the direct computation of pressure pulses may be less reliable, especially in correspondence to heavy cavitating conditions, requiring further validations in particular when the influence of characteristics such as rake distribution, hardly addressed in literature also from the experimental point of view, are considered. Cavitation tunnel test, BEM and RANS calculations have been consequently carried out for two propellers, designed for the same functioning conditions with different rake distributions, in order to stress the capabilities and the limitations of the numerical approaches in dealing with cavitation, pressure pulses predictions and the capability to discriminate between slightly different geometries in the light of their possible application in a design by optimization procedure

Molecular and functional interactions between tumor necrosis factor-alpha receptors and the glutamatergic system in the mouse hippocampus : implications for seizure susceptibility

Tumor necrosis factor (TNF)-alpha is a proinflammatory cytokine acting on two distinct receptor subtypes, namely p55 and p75 receptors. TNF-alpha p55 and p75 receptor knockout mice were previously shown to display a decreased or enhanced susceptibility to seizures, respectively, suggesting intrinsic modifications in neuronal excitability. We investigated whether alterations in glutamate system function occur in these naive knockout mice with perturbed cytokine signaling that could explain their different propensity to develop seizures. Using Western blot analysis of hippocampal homogenates, we found that p55(-/-) mice have decreased levels of membrane GluR3 and NR1 glutamate receptor subunits while GluR1, GluR2, GluR6/7 and NR2A/B were unchanged as compared to wild-type mice. In p75(-/-) mice, GluR2, GluR3, GluR6/7 and NR2A/B glutamate receptor subunits were increased in the hippocampus while GluR1 and NR1 did not change. Extracellular single-cell recordings of the electrical activity of hippocampal neurons were carried out in anesthetized mice by standard electrophysiological techniques. Microiontophoretic application of glutamate increased the basal firing rate of hippocampal neurons in p75(-/-) mice versus wild-type mice, and this effect was blocked by 2-amino-5-phosphopentanoic acid and 6-nitro-7-sulfamoyl-benzo(f)quinoxaline-2,3-dione denoting the involvement of N-methyl-D-aspartic acid and AMPA receptors. In p55(-/-) mice, hippocampal neurons responses to glutamate were similar to wild-type mice. Spontaneous glutamate release measured by in vivo hippocampal microdialysis was significantly decreased only in p55(-/-) mice. No changes were observed in KCl-induced glutamate release in both receptor knockout mice strains versus wild-type mice. These findings highlight specific molecular and functional interactions between p55 and p75 receptor-mediated signaling and the glutamate system. These interactions may be relevant for controlling neuronal excitability in physiological and pathological conditions.peer-reviewe

The activation of the cannabinoid receptor type 2 reduces neutrophilic protease-mediated vulnerability in atherosclerotic plaques

Aims The activation of cannabinoid receptor type 2 (CB2)-mediated pathways might represent a promising anti-atherosclerotic treatment. Here, we investigated the expression of the endocannabinoid system in human carotid plaques and the impact of CB2 pharmacological activation on markers of plaque vulnerability in vivo and in vitro. Methods and results The study was conducted using all available residual human carotid tissues (upstream and downstream the blood flow) from our cohort of patients symptomatic (n = 13) or asymptomatic (n = 27) for ischaemic stroke. Intraplaque levels of 2-arachidonoylglycerol, anandamide N-arachidonoylethanolamine, N-palmitoylethanolamine, N-oleoylethanolamine, and their degrading enzymes (fatty acid amide hydrolase and monoacylglycerol lipase) were not different in human plaque portions. In the majority of human samples, CB1 (both mRNA and protein levels) was undetectable. In downstream symptomatic plaques, CB2 protein expression was reduced when compared with asymptomatic patients. In these portions, CB2 levels were inversely correlated (r = −0.4008, P = 0.0170) with matrix metalloprotease (MMP)-9 content and positively (r = 0.3997, P = 0.0174) with collagen. In mouse plaques, CB2 co-localized with neutrophils and MMP-9. Treatment with the selective CB2 agonist JWH-133 was associated with the reduction in MMP-9 content in aortic root and carotid plaques. In vitro, pre-incubation with JWH-133 reduced tumour necrosis factor (TNF)-α-mediated release of MMP-9. This effect was associated with the reduction in TNF-α-induced ERK1/2 phosphorylation in human neutrophils. Conclusion Cannabinoid receptor type 2 receptor is down-regulated in unstable human carotid plaques. Since CB2 activation prevents neutrophil release of MMP-9 in vivo and in vitro, this treatment strategy might selectively reduce carotid vulnerability in human