A Short communication on Pichia pastoris vs. E. coli: Efficient expression system

One of the major challenges for vaccine producing companies is having favourable conditions for efficient expression system for living organisms in order to produce biologicals. Several companies across the globe looking for several alternative sources for better yield through efficient expression based system

Adsorption of Heparin-Binding Fragments of Fibronectin onto Hydrophobic Surfaces

Fibronectin is a multi-domain, extracellular matrix protein that plays a number of biological roles. As the adsorption of fibronectin onto the surface of implanted devices can lead to an inflammatory response or bacterial colonisation, understanding the interaction of fibronectin with material surfaces is important in the design of materials for biomedical applications. This, however, relies on having knowledge of the molecular-scale behaviour of proteins, which is difficult to investigate experimentally. In this paper, we used molecular dynamics simulations to investigate the adsorption of heparin-binding fibronectin domains onto hydrophobic surfaces. Despite the high similarity between these, their adsorption differs both in terms of the strength and the specificity of this, indicating that relatively small changes in protein structure can lead to significant changes in adsorption behaviour. This suggests that the interplay between protein structure and surface chemistry is vital for understanding the protein adsorption process and the design of novel biomaterials