The duality between care and education in a nursery daily activities

This article has the as objective to verify concepts related to care and to educate, with nursery professionals. Some documents about children education were studied and some interviews and observations of the work were done with seven nursery professionals. The main focus were the activities related to care developed with children. Data were organized following the degrees: function of the nursery, education and care concepts. Data were submitted of quality analysis, allowing the confrontation between the theorical and empirical material. The results showed that official documents do not worry about the phase under discussion, letting inaccurate the relation between the activities of care and its educational role. This inexactness is noticed in the nursery professionals' speech. The activities of care are enclosed in child education, once in the vision of the professionals it has a corrective function. This aspect is justified by the lack of formation and by the fact of they entail this activity to their mothers' experience.Esse trabalho objetivou verificar conceitos relativos ao cuidar e educar, junto a profissionais de berçário. Foram estudados documentos sobre educação infantil e realizadas entrevistas e observações da prática de sete profissionais de berçários, com enfoque nas atividades de cuidados desenvolvidas com as crianças. Os dados foram organizados segundo categorias: função do berçário, conceito de educar e de cuidar e sofreram análise qualitativa, permitindo a confrontação entre o material empírico e o teórico. Os resultados mostraram que os documentos oficiais pouco discutem a fase em questão, deixando imprecisa a relação entre as atividades de cuidado e seu papel educacional. Essa imprecisão se reflete no discurso das profissionais do berçário. As atividades de cuidados passam a estar contidas na educação da criança, na medida em que, na visão das profissionais, têm função disciplinadora. Esse aspecto justifica-se pela falta de formação e pelo fato de vincularem esta atividade às suas experiências de mães

PROTÓTIPO DE UM AEROGERADOR PARA USO DIDÁTICO

O presente trabalho visa demonstrar o funcionamento e as possibilidades de utilização didática de um protótipo de um aerogerador. A confecção do protótipo dividiu-se em três etapas: (1) estudo e projeto; (2) montagem e testes; (3) reforma do protótipo. As duas primeiras etapas foram desenvolvidas no módulo III do curso de PROEJA em Eletromecânica, na unidade curricular Projeto Integrador III, cujo tema é Agroenergias, Energias Renováveis e Meio Ambient

Prognostic Significance and Gene Expression Profiles of p53 Mutations in Microsatellite-Stable Stage III Colorectal Adenocarcinomas

Although the prognostic value of p53 abnormalities in Stage III microsatellite stable (MSS) colorectal cancers (CRCs) is known, the gene expression profiles specific to the p53 status in the MSS background are not known. Therefore, the current investigation has focused on identification and validation of the gene expression profiles associated with p53 mutant phenotypes in MSS Stage III CRCs. Genomic DNA extracted from 135 formalin-fixed paraffin-embedded tissues, was analyzed for microsatellite instability (MSI) and p53 mutations. Further, mRNA samples extracted from five p53-mutant and five p53-wild-type MSS-CRC snap-frozen tissues were profiled for differential gene expression by Affymetrix Human Genome U133 Plus 2.0 arrays. Differentially expressed genes were further validated by the high-throughput quantitative nuclease protection assay (qNPA), and confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) and by immunohistochemistry (IHC). Survival rates were estimated by Kaplan-Meier and Cox regression analyses. A higher incidence of p53 mutations was found in MSS (58%) than in MSI (30%) phenotypes. Both univariate (log-rank, P = 0.025) and multivariate (hazard ratio, 2.52; 95% confidence interval, 1.25–5.08) analyses have demonstrated that patients with MSS-p53 mutant phenotypes had poor CRC-specific survival when compared to MSS-p53 wild-type phenotypes. Gene expression analyses identified 84 differentially expressed genes. Of 49 down-regulated genes, LPAR6, PDLIM3, and PLAT, and, of 35 up-regulated genes, TRIM29, FUT3, IQGAP3, and SLC6A8 were confirmed by qNPA, qRT-PCR, and IHC platforms. p53 mutations are associated with poor survival of patients with Stage III MSS CRCs and p53-mutant and wild-type phenotypes have distinct gene expression profiles that might be helpful in identifying aggressive subsets