A Short Study in Perspective on Enhancing Communication Skills (LSRW) for English Language Learners Through Select Journalistic-Print Articles & Radio Broadcasting

The paper’s aim is to reach out to the inner most circles of rural India with the specific purpose of enhancing English language learning through usage of print and radio media by English language enhancers. Reportage in English world over has made tremendous impact for over a century now. The prominence of English as a link language to connect masses across all corners in India has undergone tectonic shifts since gaining Independence in August 15, 1947. In the paper, I have taken various articles published in English that are of national importance drawn between the years 1959 and 2009. During these times, India has massively progressed on the lines of agriculture, economy, defense, education and industrialization. During these tumultuous times, significant contribution has been made by English Reportage to abridge India and the rest of the world as well. English Reportage enhances LSRW Skills in English Language much needed for both current generation language learners and the next. Listening (through Radio and TV Dispatches), Speaking (To Communicate – for English Language Learners), Reading (Print Media) Writing (To Practice and Publish). Reportage in English as an employable skill during the first decades post Independent India was only exclusive to the ones who had access to university education or to the ones who went abroad and returned home armed with degrees. But, since the beginning of the 21st century, it has been considered as the dawn of Information Age in India. And along with universalization of education and vast increase in the circulation of English Newspapers, current generation of learners in schools, colleges and researchers have found scope in English as a language as strong medium of communication and as a tool for learning for the upcoming new generation of learners as well. English Language learners till today find journalism as a medium to not just read or hear from it but also contribute toward it

Knowledge And Awareness on Iron Deficiency Anaemia Among Women - A Questionnaire Based Survey

Iron deficiency anaemia is one of the major health problems faced by women. Several factors such as age, sex, socio-economic status, educational level play prominent roles in influencing the health status.Iron deficiency anaemia may result in slow growth and development, fatigue, stillbirth etc.But the knowledge and awareness towards it was significantly low.The main purpose of this study was to evaluate the knowledge and awareness of women towards iron deficiency anaemia. A questionnaire based survey was conducted among 100 female participants. Questionnaire was distributed through a survey link. Results were collected and analysed using IBM SPSS Statistical Analyzer (23.0 version). Frequency distribution and descriptive analysis were carried out. Pearson Chi square test was done. P value less than or equal to 0.05 was considered to be statistically significant.The results of the study showed that the knowledge and awareness of women carried according to their level of education. Participants who are educated were found to have re knowledge and awareness compared to the participants who are not educated. Therefore, it is of utmost importance for women to gain adequate knowledge on iron deficiency anaemia to make lifestyle modifications for healthy living.Saveetha Dental Colleg

Mesenchymal Stem Cells: A Future Option for Intervening Disease Management

Regeneration, revitalizing and reversal (RRR) are the primordial functions of the stem cells in the field of regenerative medicine. Though there are several cases of successful stem cell transplantation the reversal of metabolic diseases and the acquired secondary complications like chronic renal failure, neuropathy, stroke or vascular diseases are not well studied. The transplanted cells in many cases failed to home or graft in the host with no reason to attribute for such failures. Therefore, it becomes necessary to address these secondary complications with cellular therapy. The oxidative stress of the cells and tissues are attributed to the hostile microenvironment, not suitable for the survival of newly recruited cells. From our few animal studies and published literatures sources elsewhere, we foresee a huge potential for using mesenchymal stem cells (MSCs) to initially combat the secondary cardiovascular and neuronal complications in the management of the metabolic diseases. However, not all the stem cells have been tested in these lines, and further we do not know, whether all the progenitor cells from various sources and origin will behave like MSCs, which needs to be studied extensively

Enhancement of security and handling the inconspicuousness in IoT using a simple size extensible blockchain

Blockchain technology is increasingly used worldwide to enhance the performance and profit of any environment through its defining characteristics, such as security, auditability, immutability, and inconspicuousness. Owing to these characteristics, the blockchain can be used in various non-financial operations of some domains, such as the Internet of Things (IoT) and distributed computing. However, implementing blockchain technology in IoT is not always a feasible solution because blockchain deployment is costly, it has limited extensibility and provides irregular bandwidth and latency. In this regard, a simple size extensible (SSE) blockchain has been proposed to provide an optimal solution for IoT environments by satisfying the needs of the IoT environment as well as ensuring end-to-end security. The implementation of the proposed blockchain develops an overlay network to obtain a distributed environment where the blockchain is handled by the resources present therein. Two novel algorithms were introduced into the proposed system to minimize the irregularity and latency on one hand, and to maximize the throughput of the system on the other. The shared-time depending agreement algorithm (STD) minimizes the irregularity in the extraction operation and latency. The other, the shared throughput administration algorithm (STA) justifies the overall collection of the transmission load in the network and maintains the performance of the blockchain. The proposed system was applied to smart home IoT appliances to test the performance of the proposed system. The experimental results show that the proposed blockchain system minimizes nearly 70% of the data irregularity, latency, and furthermore, 30% of the blockchain extensibility is maximized as compared to the existing systems

The ubiquitin ligase CHIP integrates proteostasis and aging by regulation of insulin receptor turnover

Aging is attended by a progressive decline in protein homeostasis (proteostasis), aggravating the risk for protein aggregation diseases. To understand the coordination between proteome imbalance and longevity, we addressed the mechanistic role of the quality-control ubiquitin ligase CHIP, which is a key regulator of proteostasis. We observed that CHIP deficiency leads to increased levels of the insulin receptor (INSR) and reduced lifespan of worms and flies. The membrane-bound INSR regulates the insulin and IGF1 signaling (IIS) pathway and thereby defines metabolism and aging. INSR is a direct target of CHIP, which triggers receptor monoubiquitylation and endocytic-lysosomal turnover to promote longevity. However, upon proteotoxic stress conditions and during aging, CHIP is recruited toward disposal of misfolded proteins, reducing its capacity to degrade the INSR. Our study indicates a competitive relationship between proteostasis and longevity regulation through CHIP-assisted proteolysis, providing a mechanistic concept for understanding the impact of proteome imbalance on aging

Ubiquitylation Pathways In Insulin Signaling and Organismal Homeostasis

The insulin/insulin-like growth factor-1 (IGF-1) signaling (IIS) pathway is a pivotal genetic program regulating cell growth, tissue development, metabolic physiology, and longevity of multicellular organisms. IIS integrates a fine-tuned cascade of signaling events induced by insulin/IGF-1, which is precisely controlled by post-translational modifications. The ubiquitin/proteasome-system (UPS) influences the functionality of IIS through inducible ubiquitylation pathways that regulate internalization of the insulin/IGF-1 receptor, the stability of downstream insulin/IGF-1 signaling targets, and activity of nuclear receptors for control of gene expression. An age-related decline in UPS activity is often associated with an impairment of IIS, contributing to pathologies such as cancer, diabetes, cardiovascular, and neurodegenerative disorders. Recent findings identified a key role of diverse ubiquitin modifications in insulin signaling decisions, which governs dynamic adaption upon environmental and physiological changes. In this review, we discuss the mutual crosstalk between ubiquitin and insulin signaling pathways in the context of cellular and organismal homeostasis

In Vitro Analysis of E3 Ubiquitin Ligase Function

The covalent attachment of ubiquitin (Ub) to internal lysine residue(s) of a substrate protein, a process termed ubiquitylation, represents one of the most important post-translational modifications in eukaryotic organisms. Ubiquitylation is mediated by a sequential cascade of three enzyme classes including ubiquitin-activating enzymes (E1 enzymes), ubiquitin-conjugating enzymes (E2 enzymes), and ubiquitin ligases (E3 enzymes), and sometimes, ubiquitin-chain elongation factors (E4 enzymes). Here, in vitro protocols for ubiquitylation assays are provided, which allow the assessment of E3 ubiquitin ligase activity, the cooperation between E2-E3 pairs, and substrate selection. Cooperating E2-E3 pairs can be screened by monitoring the generation of free poly-ubiquitin chains and/or auto-ubiquitylation of the E3 ligase. Substrate ubiquitylation is defined by selective binding of the E3 ligase and can be detected by western blotting of the in vitro reaction. Furthermore, an E2 similar to Ub discharge assay is described, which is a useful tool for the direct assessment of functional E2-E3 cooperation. Here, the E3-dependent transfer of ubiquitin is followed from the corresponding E2 enzyme onto free lysine amino acids (mimicking substrate ubiquitylation) or internal lysines of the E3 ligase itself (auto-ubiquitylation). In conclusion, three different in vitro protocols are provided that are fast and easy to perform to address E3 ligase catalytic functionality