Egyes környezeti terhelések (kadmium, króm, nikkel, ólom) hatásának vizsgálata baromfi embrió modellben különös tekintettel a lipidperoxidációra és a glutation redox rendszer működésére = Investigation of some environmental loading (cadmium, chromium, nickel, lead) in chicken embryo model system with special attention to the peroxidation and the glutathione redox system

A fémterheléseket az inkubáció előtt alkalmazott egyszeri fürösztéssel, illetve a légkamrába történő injek-tálásos technikával idéztük elő kadmium, króm és nikkel vízoldható sóinak szubletális dózisaival. Megállapítottuk, hogy a fürösztéses módszerrel végzett fémterhelés nem idézett elő szignifikáns morfológiai elváltozásokat a csirke embrióban. A kezelések hatására az MDA tartalom nőtt, a GSHPx aktivitás pedig csök-kent, ami oxidatív stressz folyamatokra utal. Az injektálással végzett kezelések hatására nem szignifikánsan nőtt az elhalt embriók és a morfológiai elváltozások aránya. Nikkel hatására a GSH koncentráció szignifikán-san kisebb, a GSHPx aktivitás pedig nagyobb volt a kontroll-hoz viszonyítva. A változások fokozott lipid per-oxidációra utalnak, amelyet az antioxidáns rendszer ha-tékonyan kivédett. Kadmium hatására a MDA kon-centráció és a GSHPx aktivitás szignifikánsan nőtt, amely fokozott lipidperoxidáció mellett az antioxidáns védelmi rendszer fokozott aktivitására utal. A króm hatására az MDA tartalom nőtt, a GSHPx aktivitás pedig csökkent, ami a glutation redox rendszer terhelését jelzi. Az injektálásos módszerrel kezelt csirkékkel vég-zett nevelési kísérletben a króm és a nikkel terhelt csoportokban a kis állatlétszám miatt csak előzetes ered-ményeket kaptunk, amelyek még a kelést követően is jelentős oxidatív stresszre és a glutation rendszer ki-merülésére mutatnak. A kadmium terhelés a nevelési kísérlet során nem mutatott számottevő változásokat. | Metal-load was carried out using single bathing or single injection into air cell before incubation with sub-le-thal doses of water-soluble salts of cadmium, chromium and nickel. It was found that metal-load with bath-ing method did not cause significant morphological changes in the chicken embryo. The treat-ments caused increase of MDA content and decrease of GSHPx activity as which suggest oxidative stress processes. As effect of the treatments with single injection ratio of the dead embryos and morphological abnormalities increased not significantly. Nickel caused decrease in the GSH content and increase in GSHPx activity, which suggests improvement in lipid peroxidation which was defence by the antioxidant system effectively. Cadmium caused significant increase in both MDA content and GSHPx activity which suggest improvement in lipid peroxidation and higher activity of the antioxidant defence. Chromium caused higher MDA content and lower GSHPx activity, which suggests the exhaustion of the glutathione redox system. In the rearing experi-ment with chicken hatched from the eggs treated with injection method in the case of chromium and nickel loading only preliminary results were obtained because of the low number of viable animals. The results suggests oxidative stress and exhaustion of the glutathione system even after hatching. Cadmium did not cause measurable effects during the rearing period

Concordance of the spectral properties of dorsal wing scales with the phylogeographic structure of European male Polyommatus icarus butterflies

Abstract The males of more than 80% of the Lycaenidae species belonging to the tribe Polyommatini exhibit structural coloration on their dorsal wing surfaces. These colors have a role in reinforcement in prezygotic reproductive isolation. The species-specific colors are produced by the cellular self-assembly of chitin/air nanocomposites. The spectral position of the reflectance maximum of such photonic nanoarchitectures depends on the nanoscale geometric dimensions of the elements building up the nanostructure. Previous work showed that the coloration of male Polyommatus icarus butterflies in the Western and Eastern Palearctic exhibits a characteristic spectral difference (20 nm). We investigated the coloration and the de novo developed DNA microsatellites of 80 P. icarus specimens from Europe from four sampling locations, spanning a distance of 1621 km. Remarkably good concordance was found between the spectral properties of the blue sexual signaling color (coincident within 5 nm) and the population genetic structure as revealed by 10 microsatellites for the P. icarus species

Pest categorisation of Aleurocanthus spp

The Panel on Plant Health performed a pest categorisation of Aleurocanthus spp., a well-de fi ned insect genus of the white fl y family Aleyrodidae (Arthropoda: Hemiptera). Dif fi culties within the taxonomy of the genus give doubt about the ability to accurately identify some members to species level. Nevertheless, the genus is thought to currently include about ninety species mainly reported from tropical and subtropical areas. The genus is listed in Council Directive 2000/29/EC and is regulated on Citrus, Fortunella and Poncirus . Several Aleurocanthu s species are highly polyphagous; Aleurocanthu s spiniferus has hosts in 38 plant families; Aleurocanthu s woglumi has more than 300 hosts including Pyrus , Rosa and Vitis vinifera as well as Citrus . A. spiniferus is present in the EU in restricted areas of Italy and Greece, where it is under of fi cial control. No other Aleurocanthus spp. are known to occur in the EU. Host plants for planting, excluding seeds, and cut fl owers or branches are the main pathways for entry. Outside of the EU, the genus can be found in regions that have climate types which also occur within the EU, suggesting establishment is possible. Aleurocanthus spp. can be signi fi cant pests of crops that are also grown in the EU. Phytosanitary measures are available to reduce the likelihood of entry into the EU, e.g. sourcing host plants for planting from pest free areas. As a genus Aleurocanthus does satisfy all the criteria that are within the remit of EFSA to assess and required by risk managers to give it consideration as a Union quarantine pest. Aleurocanthus does not meet all of the criteria to allow it consideration by risk managers as a Union regulated non-quarantine pest (RNQP). Speci fi cally, Aleurocanthus is not widespread in the EU

Pest categorisation of Ips amitinus

The Panelon Plant Health performed a pest categorisation of the small spruce bark beetle, Ipsamitinus (Eichhoff) (Coleoptera: Curculionidae, Scolytinae), for the EU. I.amitinus is a well-defined and distinguishable species, native to Europe and attacking mainly spruce (Picea spp.) and pine (Pinus spp.) and sporadically fir (Abies spp.) and larch (Larix spp.). It is distributed in 16 EU Member States and is locally spreading in some. The pest is listed in Annex IIB of Council Directive 2000/29/EC. Protected zones are in place in Ireland, Greece and the United Kingdom. Wood, wood products, bark and wood packaging material are considered as pathways for this pest, which is also able to disperse by flight over tens of kilometres. The insects normally establish on fallen or weakened trees (e.g. after a fire or a drought) but can also occasionally mass-attack healthy trees, when population densities are high. The males produce pheromones that attract conspecifics of both sexes. Each male attracts one to seven females to establish a brood system; each female produces 1-60 offspring. The insects also inoculate their hosts with pathogenic fungi. There are one or two generations per year. The wide current geographic range of I.amitinus suggests that it is able to establish in most areas in the EU, including the protected zones, where its hosts are present. The damage due to I.amitinus is limited and usually does not require control. Sanitary thinning or clear-felling is the usual control methods, when necessary. Quarantine measures are implemented to prevent entry in protected zones. All criteria for consideration as a potential protected zone quarantine pest are met. The criteria for considering I.amitinus as a potential regulated non-quarantine pest are not met since plants for planting are not viewed as a pathway

Pest categorisation of Aschistonyx eppoi

The Panel on Plant Health performed a pest categorisation of the gall midge Aschistonyx eppoi Inouye (1964) (Diptera, Cecidomyiidae), for the EU. A. eppoi is a well‐defined and distinguishable species, native to Japan and Korea, and recognised as a pest of Juniperus chinensis, although our knowledge is solely based on one unique publication. A. eppoi is absent from the EU, and is listed in Annex IIAI of Directive 2000/29/EC. Its host plants, Juniperus spp. are also listed in Annex III of Directive 2000/29/EC. Plants for planting and branches are considered as pathways for this pest. A. eppoi has been intercepted twice (1974; 1975) in the EU and has been eradicated. The pest is likely to affect bonsai plants of J. chinensis if it were to establish in the EU territory. However, as it is unknown whether A. eppoi would attack the Juniperus spp. that occur in the EU, its potential impact on the wild vegetation is also unknown. As the pest originates from areas with warm climates, impact outdoors would affect the southern parts of the EU. Cultural control (destruction of infested material) and chemical control are the major control methods. All criteria assessed by EFSA for consideration as a potential quarantine pest are met, although there are high uncertainties regarding impact. The species is presently absent from the EU, and thus the criteria for consideration as a potential regulated non‐quarantine pest are not met

Pest categorisation of Scaphoideus luteolus

The Panel on Plant Health performed a pest categorisation of Scaphoideus luteolus, a well-defined phloem sap-feeding insect species in the family Cicadellidae (Insecta: Hemiptera). It can be identified using taxonomic keys. S. luteolus is only present in the eastern part of the USA. The main host plants of S. luteolus are species of the genus Ulmus (U. americana, U. alata, U. bergmanianna, U. szechuanica, U. rubra), but specimens have also been collected on Vitis sp., Salix sp. and Populus sp. The species does not cause damage by itself, but it is the only confirmed vector of the phytoplasma Candidatus Phytoplasma ulmi (CPu), which is present in North America where it causes heavy damage to the local elms, as well as in some European countries where the local elms are considered less susceptible. S. luteolus has three developmental stages (egg, nymph, adult). It overwinters in the egg stage, takes 36–42 days to complete nymphal stage, and adults are found throughout the summer period. Both nymphs and adults are capable of transmitting CPu and, after acquiring the pathogen, remain infective for the rest of their life. The main pathways are cut branches and plants for planting. These pathways are not regulated for the main host, Ulmus, though requirements are in place in relation to other pests on Ulmus. These pathways are also not regulated for Salix. Establishment would be favoured by the wide coverage of Ulmus spp. in the EU territory and by climatic conditions comparable to those of the pest’s native range. S. luteolus meets all the criteria assessed by EFSA for consideration as potential Union quarantine pest. The criteria for considering it as a potential Union regulated non-quarantine pest are not met since the species is absent from the EU

Pest categorisation of non-EU Acleris spp

The Panel on Plant Health performed a pest categorisation of non-EU Acleris spp. Acleris is a welldefined insect genus in the family Tortricidae (Insecta: Lepidoptera). Species can be identified using taxonomic keys based on adult morphology and genitalia. The genus includes 261 species attacking conifers and non-conifer plants in many areas in the world, among which 40 species are present in the EU. The non-EU species are collectively listed in Annex IAI of Council Directive 2000/29/EC as Acleris spp. (non-European). Some species are important defoliators in North America, mainly on conifers but also on several broadleaf trees. Females lay eggs on the leaves or on the bark. The larvae bind together with silk the leaves upon which they feed. Pupation occurs in leaves attached with silk or in the soil. Some species are univoltine; others are bivoltine or multivoltine. Flight capacity is not documented, but outbreak expansion suggests that the adults can probably fly long distances. The main pathways for entry are host plants for planting with or without soil, cut branches, fruits of host plants (including cones), round wood with bark and bark. The presence of host plants and suitable EU climate would allow the establishment of the known non-EU harmful species. In the literature, nine non-EU Acleris species are reported as pests on various host plants, namely A. gloverana, A. variana, A. minuta, A. nishidai, A. issikii, A. semipurpurana, A. robinsoniana, A. senescens and A. nivisellana. These non-EU Acleris spp. satisfy all the criteria to be considered as Union quarantine pests. Concerning the other 212 non-EU Acleris species, there is scarce information on host plants, pests status and climatic suitability. Measures are in place to prevent the introduction of non-EU Acleris spp. through the pathways described in the document. As non-EU Acleris spp. are not present in the EU and plants for planting are not the major pathway for spread, non-EU Acleris spp. do not meet the criteria to be considered as regulated non-quarantine pests

Pest categorisation of Pseudopityophthorus minutissimus and P. pruinosus

The Panel on Plant Health performed a pest categorisation of Pseudopityophthorus minutissimus and Pseudopityophthorus pruinosus, two well-defined insect species in the family Curculionidae, subfamily Scolytinae (Insecta: Coleoptera). They can be identified using taxonomic keys. P. minutissimus is present in parts of Canada and the USA, and P. pruinosus is present in parts of the USA, Guatemala, Honduras and Mexico. The main host plants of the two species are Quercus spp., but they also attack several other genera. The two species mostly colonise weakened or dead branches but can also attack the stems. They are mostly secondary pests but they vector the oak wilt fungus, Bretziella fagacearum, which causes heavy damage in American Quercus spp. populations. The fungus is mainly transmitted by the young adults during their maturation feeding on twigs, leaf petioles and young acorn stems. The beetles are polygamous and have two generations per year in most of their range. The main pathways are wood, bark, plants for planting, cut branches, chips and wood waste. These pathways are fully or partly regulated for the genera Quercus, Castanea and Prunus. However, the pathways are not regulated for the following genera: Carpinus, Fagus, Hamamelis, Alnus. P. minutissimus and P. pruinosus meet all the criteria assessed by EFSA for consideration as potential Union quarantine pest. The criteria for considering P. minutissimus and P. pruinosus as potential Union regulated non-quarantine pests are not met since neither species are known to be present in the EU

Pest categorisation of Arrhenodes minutus

The Panel on Plant Health performed a pest categorisation of Arrhenodes minutus, a well-defined wood-boring insect species in the family Brentidae (Insecta: Coleoptera). It can be identified using taxonomic keys. A. minutus is only present in southern Canada and eastern USA down to Florida. The main host plants of A. minutus are species of the genera Quercus, Ulmus, Fagus and Populus. The pest larvae bore galleries in the wood, causing structural damage to the timber. The pest is also a vector of the quarantine pest Breziella (Ceratocystis) fagacearum. A. minutus most often lays its eggs in wounded parts of the trees where sap is oozing. The female bores minute holes with her snout and deposits one egg in each of them. The larvae bore a straight gallery against the grain. When the gallery nearly reaches the other side of the bole, it makes a sharp U-turn towards the point of origin. These galleries cause structural damage to the timber. The life cycle lasts generally 3 years, but some individuals develop in 2 years and a few require 4 years. The main pathways are wood and possibly plants for planting. Specific phytosanitary requirements exist for Quercus and Populus only, while Ulmus is regulated in relation to other pests. Establishment would be favoured by the wide distribution of host trees in the EU territory and by climatic conditions locally comparable to those of the pest’s native range. A. minutus meets all the criteria assessed by EFSA for consideration as potential Union quarantine pest. The criteria for considering it as a potential Union regulated non-quarantine pest are not met since the species is absent from the EU

Pest categorisation of Cephalcia lariciphila

The Panel on Plant health performed a pest categorisation of the larch web‐spinning sawfly Cephalcia lariciphila (Hymenoptera: Pamphiliidae) for the EU. The insect has been reported in 11 EU Member States (MSs). It is a quarantine pest listed in Annex IIB of Council Directive 2000/29/EC. Protected zones are in place in Ireland and the UK (Northern Ireland, Isle of Man and Jersey). C. lariciphila can feed on all species of the genus Larix. There have been reported outbreaks in the Czech Republic, Germany, the Netherlands and the UK (England and Wales) in plantations of European larch (Larix decidua) and Japanese larch (Larix kaempferi = Larix leptolepis). C. lariciphila is absent in the protected zones. The pest can enter the protected zones by human‐assisted spread or by natural spread from EU areas where the pest is present. Plants for planting are considered the most important pathway for the pest. The pest can establish in the protected zones because the climatic conditions are similar to those of the 11 MSs where C. lariciphila is established, and the pest's main host plants are present. The prepupae overwinter in the litter, the adults emerge during May–June, and each female lays 30–40 eggs in slits in mature needles. The larvae feed on the needles through four instars. There is one generation per year; some of the prepupae undergo prolonged diapause for more than 1 year. The impact where the pest occurs is mainly related to the loss of tree growth following defoliation, while tree mortality was locally observed only after repeated defoliation. However, impact is likely to be mitigated by local biological control agents. All criteria assessed by EFSA above for consideration as a potential protected zone quarantine pest and as a potential regulated non‐quarantine pest were met