Clinical repercussions of Martin-Gruber anastomosis: anatomical study

AbstractObjectiveThe main objective of this study was to describe Martin-Gruber anastomosis anatomically and to recognize its clinical repercussions.Method100 forearms of 50 adult cadavers were dissected in an anatomy laboratory. The dissection was performed by means of a midline incision along the entire forearm and the lower third of the upper arm. Two flaps including skin and subcutaneous tissue were folded back on the radial and ulnar sides, respectively.ResultsNerve communication between the median and ulnar nerves in the forearm (Martin-Gruber anastomosis) was found in 27 forearms. The anastomosis was classified into six types: type I: anastomosis between the anterior interosseous nerve and the ulnar nerve (n=9); type II: anastomosis between the anterior interosseous nerve and the ulnar nerve at two points (double anastomosis) (n=2); type III: anastomosis between the median nerve and the ulnar nerve (n=4); type IV: anastomosis between branches of the median nerve and ulnar nerve heading toward the flexor digitorum profundus muscle of the fingers; these fascicles form a loop with distal convexity (n=5); type V: intramuscular anastomosis (n=5); and type VI: anastomosis between a branch of the median nerve to the flexor digitorum superficialis muscle and the ulnar nerve (n=2).ConclusionKnowledge of the anatomical variations relating to the innervation of the hand has great importance, especially with regard to physical examination, diagnosis, prognosis and surgical treatment. If these variations are not given due regard, errors and other consequences will be inevitable

Caracterização agronômica e edafoclimática dos vinhedos de elevada altitude

The objective of this research was to perform the agronomic and edaphoclimatic characterization of vineyards and to determine cultivation trends for the main varieties employed in the production of high altitude fine wines in Santa Catarina State, Brazil. High altitude vineyards were considered those vineyards located 900 m above sea level and vineyards belonging to members of the Santa Catarina Association of Altitude Fine Wines located below 900 m altitude. To assess cultivation trends, the vineyards were geo-referenced, following the Embrapa Uva e Vinho guidelines. To perform the edaphoclimatic characterization, the vineyard polygons were overlaid with physiographic, climatic and soil maps in a Geographic Information System. The characterization of the high altitude vineyards was based on planted area. The varieties with greatest areas were Cabernet Sauvignon (37.9%), Merlot (14.5%) Chardonnay (8.0%) and Sauvignon Blanc (7.5%). From 2009 to 2013 vineyards with C. Sauvignon were replaced for white varieties and for Pinot Noir, especially in altitudes over 1,100 m. A trend in the use of white varieties and Pinot Noir in new vineyards was identified, but the main trend was the increase of experimental areas, with additional 60 varieties of red and white grapes. In relation to physiography, the norm of the Acavitis requires that the vineyards of their associates be located above 900 m of altitude. However, vineyards were found at altitudes ranging from 675 m to 1,452 m, with 64.6% of the area found between altitudes ranging from 1,100 to 1,300 m above sea level. The annual rainfall was 240% higher than the values considered optimal described in the literature for high altitude vineyards. The average annual temperature ranged between 12 °C and 18 °C and both the temperature range as the annual hours of sunshine were considered sufficient to complete the cycle of all varieties.O objetivo desse trabalho foi realizar a caracterização agronômica e edafoclimática dos vinhedos e determinar as tendências de uso das principais variedades para produzir vinhos finos nas regiões de elevada altitude do Estado de Santa Catarina. Foram considerados vinhedos de elevada altitude os vinhedos localizados acima de 900 m em relação ao nível do mar e os vinhedos pertencentes aos associados da Associação Catarinense de Vinhos Finos de Altitude (Acavitis) localizados em altitudes inferiores a 900 m. Para avaliar as tendências de cultivo foi realizado um cadastro georreferenciado, seguindo as normas da Embrapa Uva e Vinho. Para realizar a caracterização edafoclimática, as áreas dos vinhedos foram cruzadas com mapas fisiográficos, climáticos e pedológicos em um Sistema de Informações Geográficas. A caracterização dos vinhedos de elevada altitude foi realizada com base na área plantada. As variedades com maior área foram Cabernet Sauvignon (37,9%), Merlot (14,5%), Chardonnay (8,0%) e Sauvignon Blanc (7,5%). De 2009 a 2013 houve substituição de C. Sauvignon por variedades brancas e Pinot Noir, principalmente nas altitudes superiores a 1.100 m. Houve uma tendência no uso de variedades brancas e Pinot Noir em vinhedos novos, mas a principal tendência observada foi no crescimento de áreas experimentais, com outras 60 variedades tintas e brancas. Em relação à fisiografia, a norma da Acavitis exige que os vinhedos dos associados estejam localizados acima de 900 m de altitude, porém foram encontrados vinhedos em altitudes entre de 675 m e 1.452 m, com 64,6% da área plantada entre 1.100 e 1.300 m acima do nível do mar. Os vinhedos estão em áreas onde a pluviosidade anual chegou a ser 240% superior aos valores considerados ótimos pela literatura especializada. A temperatura média anual variou entre 12 oC e 18 oC e tanto a amplitude térmica quanto as horas anuais de sol foram consideradas suficientes para completar o ciclo de todas as variedades

STUDY OF THE MEDIAN AND ULNAR NERVOUS BRANKS TO KAPLAN’S LINE

ABSTRACT Objective: This study aims to present lines A1 and A2 in association with Kaplan’s cardinal line (LCK), and relate them to the thenar motor branch of the median nerve (RMTNM) and to the deep branch of the ulnar nerve (RPNU). Methods: Ten hands of five adult cadavers were dissected. Results: The RMTNM origin was positioned proximal to the LCK in all limbs. In two, the RMTNM was positioned exactly on the A1 line; in seven, it was on the ulnar side in relation to A1. In one, it was on the radial side relative to the A1. The origin of the RPNU was identified between the pisiform and the LCK in nine limbs; in one, the RPNU was positioned from the ulnar nerve in relation to A2; and in two, the A2 passed exactly at the point of division of the ulnar nerve into superficial branches and deep. We did not identify the positioning of the RPNU on the radial side of the A2 line. Conclusion: The impact of this study was to identify the anatomical trajectory of these nerves by detaching A1 and A2 along with the KCL, avoiding iatrogenic lesions during surgical procedures. Level of Evidence IV, Case Series

Química sem fronteiras

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Chemistry in Brazil: perspectives and needs for the next decade. Introductory document

Over the past years the Brazilian Chemical Society (SBQ) has been working on different projects related to the development of Chemistry in Brazil. After a discussion throughout the country two documents have been published in Quimica Nova: Mobilizing Axes in Chemistry and The Chemist's Education. Here, we describe the initial document which was the starting point for the discussion of a new series of papers published in this special issue of Quimica Nova which presents an overview of the Chemistry in our country and the perspectives and needs for the next decade.S7S1

Efeito do duplo envelhecimento nas propriedades da liga ti-12mo-13nb

Ligas de Ti do tipo β tem sido muito estudadas para aplicação em dispositivos biomédicos por apresentarem um balanço de vantagens em relação às outras ligas de Ti, tais como alta relação resistência / densidade, excelente resistência à fadiga e, além disso, tendem a apresentar o menor módulo dentre todos os tipos de ligas. Contudo, para um material ser utilizado como implante ortopédico é necessário um balanço entre alta resistência e baixo módulo de elasticidade. Estudos mostram que não é trivial obter simultaneamente baixo módulo de elasticidade e alta resistência nas ligas de Ti. Várias microestruturas podem ser obtidas por diferentes tratamentos de envelhecimento os quais influenciam várias propriedades das ligas e, portanto um satisfatório tratamento de envelhecimento poderá resultar num melhor balanço entre baixo módulo de elasticidade e alta resistência. Há vários tratamentos de envelhecimento que podem ser realizados em uma liga β metaestável. Um destes é o duplo envelhecimento, que consiste em dois processos. Realiza-se um envelhecimento em uma temperatura em torno de 250 ºC que favoreça a precipitação da fase ω (isotérmica) e um subsequente envelhecimento numa temperatura maior para a precipitação de fase α. O foco deste envelhecimento é obter uma precipitação de fase α mais fina e uniforme. Desta forma, o objetivo deste trabalho foi estudar o efeito do duplo envelhecimento nas propriedades da liga Ti-12Mo-13Nb. A liga Ti-12Mo-13Nb foi processada termomecanicamente e passou por um duplo envelhecimento que consistiu primeiramente num envelhecimento na temperatura de 300 ᵒC por 10 min, 45 min e 3 h e um subsequente envelhecimento na temperatura de 500 ºC por 24 h. Como resultado foi observado que não houve variação significativa na dureza e no módulo de elasticidade com a variação da fração volumétrica da fase ω durante o envelhecimento prévio da temperatura externa

Erythrina velutina Willd. alkaloids : piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

Introduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. Erythrina species are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids (BIAs), which can act on several pathology-related biological targets. Objectives: In this sense, in an unprecedented approach used with a non-model Fabaceae species grown in its unique arid natural habitat, a combined transcriptome and metabolome analyses (seeds and leaves) is presented. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a NextSeq 500 platform. Regarding metabolite profiling, the High-resolution Liquid Chromatography was coupled to DAD and a micrOTOF-QII mass spectrometer by using electrospray ionization (ESI) and Time of Flight (TOF) analyzer. The tandem MS/MS data were processed and analyzed through Molecular Networking approach. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids, several of them unique. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: Overall, these results could contribute by indicating potential biotechnological targets formodulation of erythrina alkaloids biosynthesis as well as improve molecular databases with omic data from a non-model medicinal plant, and reveal an interesting chemical diversity of Erythrina BIA harvested in Caatinga

Erythrina velutina Willd. alkaloids: Piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

Introduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. The scarce studies regarding non-model plants impair advances in this field. Erythrina spp. are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids, which can act on several pathology-related biological targets. Objective: Herein the purpose is to employ combined transcriptome and metabolome analyses (seeds and leaves) of a non-model medicinal Fabaceae species grown in its unique arid natural habitat. The study tries to propose a putative biosynthetic pathway for the bioactive alkaloids by using an omic integrated approach. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a Illumina NextSeq 500 platform. Regarding the targeted metabolite profiling, Nuclear Magnetic Resonance and the High-Performance Liquid Chromatography coupled to a micrOTOF-QII, High Resolution Mass Spectrometer, were used. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids by metabolome tools. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: These results contribute by indicating potential biotechnological targets Erythrina alkaloids biosynthesis as well as to improve molecular databases with omic data from a non-model medicinal plant. Furthermore, they reveal an interesting chemical diversity in Erythrina velutina harvested in Caatinga. Last, but not least, this data may also contribute to tap Brazilian biodiversity in a rational and sustainable fashion, promoting adequate public policies for preservation and protection of sensitive areas within the Caatinga