Interplay between n-3 and n-6 long-chain polyunsaturated fatty acids and the endocannabinoid system in brain protection and repair.

The brain is enriched in arachidonic acid (ARA) and docosahexaenoic acid (DHA), long-chain polyunsaturated fatty acids (LCPUFA) of the n-6 and n-3 series, respectively. Both are essential for optimal brain development and function. Dietary enrichment with DHA and other long-chain n-3 PUFA, such as eicosapentaenoic acid (EPA) have shown beneficial effects on learning and memory, neuroinflammatory processes and synaptic plasticity and neurogenesis. ARA, DHA and EPA are precursors to a diverse repertoire of bioactive lipid mediators, including endocannabinoids. The endocannabinoid system comprises cannabinoid receptors, their endogenous ligands, the endocannabinoids, and their biosynthetic and degradation enzymes. Anandamide (AEA) and 2-archidonoylglycerol (2-AG) are the most widely studied endocannabinoids, and are both derived from phospholipid-bound ARA. The endocannabinoid system also has well established roles in neuroinflammation, synaptic plasticity and neurogenesis, suggesting an overlap in the neuroprotective effects observed with these different classes of lipids. Indeed, growing evidence suggests a complex interplay between n-3 and n-6 LCPUFA and the endocannabinoid system. For example, long-term DHA and EPA supplementation reduces AEA and 2-AG levels, with reciprocal increases in levels of the analogous endocannabinoid-like DHA and EPA-derived molecules. This review summarises current evidence of this interplay and discusses the therapeutic potential for brain protection and repair

Cooperativity-Dependent Folding of Single-Stranded DNA

none5The folding of biological macromolecules is a fundamental process of which we lack a full comprehension. Mostly studied in proteins and RNA, single-stranded DNA (ssDNA) also folds, at physiological salt conditions, by forming nonspecific secondary structures that are difficult to characterize with biophysical techniques. Here, we present a helix-coil model for secondary-structure formation, where ssDNA bases are organized in two different types of domains (compact and free). The model contains two parameters: the energy gain per base in a compact domain, ϵ, and the cooperativity related to the interfacial energy between different domains, γ. We test the ability of the model to quantify the formation of secondary structure in ssDNA molecules mechanically stretched with optical tweezers. The model reproduces the experimental force-extension curves in ssDNA of different molecular lengths and varying sodium and magnesium concentrations. Salt-correction effects for the energy of compact domains and the interfacial energy are found to be compatible with those of DNA hybridization. The model also predicts the folding free energy and the average size of domains at zero force, finding good agreement with secondary-structure predictions by mfold. We envision the model could be further extended to investigate native folding in RNA and proteins.noneViader-Godoy X.; Pulido C.R.; Ibarra B.; Manosas M.; Ritort F.Viader-Godoy, X.; Pulido, C. R.; Ibarra, B.; Manosas, M.; Ritort, F

Mechanical characterization of base analogue modified nucleic acids by force spectroscopy

We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy

Experimental study of a microchannel bubble injector for microgravity applications

We perform a quantitative characterization of a microbubble injector in conditions relevant to microgravity. The injector pregenerates a slug flow by using a capillary T-junction, whose operation is robust to changes in gravity level. We address questions regarding the performance under different injection conditions. In particular we focus on the variation of both gas and liquid flow rates. The injection performance is characterized by measuring bubble injection frequency and bubble sizes. We obtain two distinct working regimes of the injector and identify the optimal performance as the crossover region between them.Peer ReviewedPostprint (published version

Experimental study of a microchannel bubble injector for microgravity applications

We perform a quantitative characterization of a microbubble injector in conditions relevant to microgravity. The injector pregenerates a slug flow by using a capillary T-junction, whose operation is robust to changes in gravity level. We address questions regarding the performance under different injection conditions. In particular we focus on the variation of both gas and liquid flow rates. The injection performance is characterized by measuring bubble injection frequency and bubble sizes. We obtain two distinct working regimes of the injector and identify the optimal performance as the crossover region between them.Peer Reviewe