Economic Regions Based on the Agglomeration Economics: an Evaluation for the Spanish Case.

What is region? From an economic point of view, a region is a unit in which capital and labour move freely and goods and services are totally open to trade with other regions without any frontiers or limitations. The openness of the regions and their interaction with other regions are their main characteristics. From a statistical point of view internal homogeneity and also heterogeneity between the regions are both desirable properties of a set of regions. The objective of this chapter is revise the concept of region and propose a division of the territory which satisfy the statistical and economical region concept requirements in the way of propose a classification that has economic theoretical meaning but, at the same time, satisfy the internal homogeneity and heterogeneity among classes. Using micro data from the latest Census available, the Spanish territory can be divided into functional regions that emphasize the importance of location and agglomeration economies (a classification with theoretical economic meaning). A set of contrast and indexes is applied to prove that, when studying labor economic issues, such classification based on economic criteria results on more convenient regions than the administrative ones commonly used

Improving motivation in pre-school education through the use of project-based learning and cooperative learning

[EN] The emphasis on skills-based learning has caused curricular change in education systems across the European Union, particularly in terms of the increased use of active teaching methodologies at all stages of education. These sorts of methodology are proven to have a positive impact on students’ motivation to learn and involve teaching strategies that represent a significant departure from traditional approaches. In this way, teachers in Pre-school Education can employ play-based strategies, which not only foster students’ acquisition of new skills but also their motivation. The objective of this study is to assess the impact of using active methodologies on the intrinsic motivation of students in the second cycle of Pre-school Education (3–6 years old) toward the process of teaching and learning.S

Mapping poverty at the local level in Europe: A consistent spatial disaggregation of the AROPE indicator for France, Spain, Portugal and the United Kingdom

In the EU, territorial inequalities in terms of income and poverty have been broadly analysed at the national and regional levels. However, mainly due to the lack of reliable data, very little attention has been paid to territorial inequalities within European regions, namely, at a more local level, such as in metropolitan areas, cities or neighbourhoods. This paper proposes a methodology to disaggregate official regional poverty figures into poverty indicators for smaller spatial units, mainly local administrative units. For each country, poverty figures at the regional level from household surveys are combined with microcensus data that contain details on the local entities of residence to disaggregate the regional poverty indicator. In contrast to previous methodologies, our proposed technique guarantees consistency between the local poverty estimates and the regional poverty figures through a second step that adjusts the initial estimates based on generalized cross entropy. The procedure is applied for four European countries: France, Spain, the United Kingdom and Portugal. The resulting local estimates provide an intraregional map of poverty and some insights into the particular behaviour of the capital regions and the disparities between city centres and their surrounding areas.En la UE, las desigualdades territoriales en cuanto a ingresos y pobreza se han analizado ampliamente a nivel nacional y regional. Sin embargo, debido principalmente a la falta de datos fiables, se ha prestado muy poca atención a las desigualdades territoriales dentro de las regiones europeas a un nivel más local, como pueden ser las zonas metropolitanas, las ciudades o los barrios. En el presente artículo se propone una metodología para desglosar las cifras regionales oficiales de pobreza en indicadores de pobreza para unidades espaciales más pequeñas, principalmente unidades administrativas locales. Para cada país, se combinan las cifras de pobreza a nivel regional procedentes de las encuestas de hogares con datos de microcensos que contienen detalles sobre las entidades locales de residencia para desglosar el indicador de pobreza regional. A diferencia de las metodologías anteriores, la técnica que se propone aquí garantiza la coherencia entre las estimaciones de pobreza locales y las cifras de pobreza regionales mediante un segundo paso que ajusta las estimaciones iniciales basadas en la entropía cruzada generalizada. El procedimiento se aplica en cuatro países europeos: Francia, España, el Reino Unido y Portugal. Las estimaciones locales resultantes proporcionan un mapa intrarregional de la pobreza y algunas percepciones del comportamiento particular de las regiones que albergan la capital y las disparidades entre los centros de las ciudades y sus zonas circundantes

Aging Uncouples Heritability and Expression-QTL in Caenorhabditis elegans

The number and distribution of gene expression QTL (eQTL) represent the genetic architecture of many complex traits, including common human diseases. We previously reported that the heritable eQTL patterns are highly dynamic with age in an N2 × CB4856 recombinant inbred population of the nematode Caenorhabditis elegans. In particular, we showed that the number of eQTL decreased with age. Here, we investigated the reason for this decrease by combining gene expression profiles at three ages in the wild types N2 and CB4856 with the reported expression profiles of the RIL population. We determined heritability and transgression (when gene expression levels in the RILs are more extreme than the parents) and investigated their relation with eQTL changes with age. Transgressive segregation was widespread but depended on physiological age. The percentage of genes with an eQTL increased with a higher heritability in young worms. However, for old worms this percentage hardly increased. Using a single marker approach, we found that almost 20% of genes with heritability >0.9 had an eQTL in developing worms. Surprisingly, only 10% was found in old worms. Using a multimarker approach, this percentage increased to almost 30% for both age groups. Comparison of the single marker to a multiple marker eQTL mapping indicated that heritable regulation of gene expression becomes more polygenic in aging worms due to multiple loci and possible epistatic interactions. We conclude that linkage studies should account for the relation between increased polygenic regulation and diminished effects at older ages

Ehlers- Danlos tipo IV

El Sd. Ehlers-Danlos tipo IV o vascular es un trastorno hereditario autosómico dominante causado por mutaciones en el gen COL3A1. Dicho gen codifica la proteína procolágeno III que forma parte del tejido conectivo. Esta alteración se manifiesta por la presencia de rasgos faciales característicos, piel translúcida, así como roturas arteriales, digestivas y uterinas por fragilidad de sus paredes. Las complicaciones pueden afectar a todas las áreas anatómicas, siendo habituales la disección de las arterias vertebrales y carótidas, perforaciones de útero durante el embarazo y perforación de colón de forma recurrente. El diagnóstico, ya que no existe ninguno específico para esta enfermedad, está basado en la clínica, en técnicas de imagen y en la identificación de mutaciones del gen COL3A1. La intervención médica está enfocada al tratamiento sintomático y a la profilaxis de las complicaciones debido a su alto índice de mortalidad tras su aparición. Se debe realizar diagnóstico diferencial principalmente con el Sd. De Marfan, Sd. De Loeys-Dietz y Sd. Ehlers-Danlos (en la edad adulta).Grado en Medicin

No evidence of biodiversity effects on stream ecosystem functioning across green and brown food web pathways

[EN] Biodiversity loss is known to affect the two fundamental and opposite processes controlling carbon and nutrient cycles globally, that is, primary production and decomposition, which are driven by green and brown food web compartments, respectively. However, biodiversity in these two food web compartments has been mostly studied independently, and potential reciprocal effects of biodiversity loss on ecosystem processes remain unclear. We conducted a 35-day stream mesocosm experiment with two levels of algal diversity (natural and diluted periphyton communities) and three levels of litter diversity (no litter, monocultures of poplar, maple, and oak, and the three-spp. mixture) to simulate changes in biodiversity in both the green and brown pathways of an aquatic food web. We then measured multiple ecosystem processes pertaining to carbon cycling. We predicted that algal diversity would enhance decomposition and sporulation of fungal decomposers, while litter diversity would enhance algal growth and net primary production, due to the more diverse algal exudates or litter nutrients being released from more diverse mixtures. In contrast to this hypothesis, we only found biodiversity effects on an ecosystem process within the green pathway: there was a relationship between algal diversity and biofilm carrying capacity. Nevertheless, we found that this relationship was affected by the presence or absence of litter (algal diversity increased the carrying capacity in presence of litter and decreased it in its absence), which also influenced the algal community structure. Our mesocosm experiment did not evidence relationships between biodiversity and ecosystem processes across different food web compartments, but further studies in more realistic conditions would be necessary to confirm this result. If supported, the lack of biodiversity-ecosystem functioning relationships across compartments would facilitate the prediction of the impacts of biodiversity loss on ecosystems.Spanish Ministry for Science, Innovation and Universities and FEDER; U.S. National Science Foundation, Grant/Award Number: 133234

Extreme temperature events alter stream ecosystem functioning

Extreme temperature events have increased in intensity, duration and frequency in the last century, with potential consequences on organisms and ecosystems. In many streams, leaf litter of terrestrial origin is a key resource for microorganisms and some detritivores, and its decomposition has a main role on ecosystem functioning and is often used as an indicator of ecological integrity. As litter is often exposed to atmospheric conditions before entering the stream, extreme warming and freezing events may alter its physicochemical structure and affect decomposition and associated detritivores. We tested this prediction in a microcosm experiment by exposing litter of three tree species (in single-species treatments and the 3-species mixture) to different temperature pre-treatments: heating (40 degrees C), freezing ( - 20 degrees C) and both (heating followed by freezing). We then examined changes in litter traits due to leaching (72 h), litter decomposition in the absence and presence of detritivores, and detritivore growth (28 d), with focus on mass and nutrient (nitrogen and phosphorus) changes. Nutrient leaching was promoted mostly by the heating pre-treatment, which apparently produced lower-quality litter. However, microbial activity mostly resulted in litter mass and nutrient gain, which were reinforced by the heating pre-treatment, while freezing had the opposite effect. When detritivores were present, decomposition showed high variation among litter types but, again, the heating and freezing pre-treatments tended to reduce and enhance nutrient loss, respectively. The greatest and more consistent effects occurred for detritivore growth, which was reduced by temperature pre-treatments, particularly in the highest-quality litter type. In general, the sequential application of heating and freezing pre-treatments showed no synergistic effect, and the litter mixture showed similar responses to single-species treatments. Our results demonstrate that short-term extreme temperatures can modify litter quality in riparian soils and have subsequent effects on its decomposition within the stream and associated fauna, potentially altering stream food webs, ecosystem functioning and biogeochemical cycles.This study was funded by the Spanish Ministry for Science, Innovation and Universities and FEDER (project BioLoss, Ref. RTI2018-095023-B400), Basque Goverment funds (Ref. IT951-16) and Initiation Fondecyt Project (Ref. 11170390). I. Diaz and U. Apodaka-Etxebarria contributed to the sample processing

Transmitted drug resistance to antiretroviral drugs in Spain during the period 2019–2021

HIV; Clinical resistance; TransmissionVIH; Resistencia clínica; TransmisiónVIH; Resistència clínica; TansmissióTo evaluate the prevalence of transmitted drug resistance (TDR) to nucleoside and nonnucleoside reverse transcriptase inhibitors (NRTI, NNRTI), protease inhibitors (PI), and integrase strand transfer inhibitors (INSTI) in Spain during the period 2019–2021, as well as to evaluate transmitted clinically relevant resistance (TCRR) to antiretroviral drugs. Reverse transcriptase (RT), protease (Pro), and Integrase (IN) sequences from 1824 PLWH (people living with HIV) were studied. To evaluate TDR we investigated the prevalence of surveillance drug resistance mutations (SDRM). To evaluate TCRR (any resistance level ≥ 3), and for HIV subtyping we used the Stanford v.9.4.1 HIVDB Algorithm and an in-depth phylogenetic analysis. The prevalence of NRTI SDRMs was 3.8% (95% CI, 2.8%–4.6%), 6.1% (95% CI, 5.0%–7.3%) for NNRTI, 0.9% (95% CI, 0.5%–1.4%) for PI, and 0.2% (95% CI, 0.0%–0.9%) for INSTI. The prevalence of TCRR to NRTI was 2.1% (95% CI, 1.5%–2.9%), 11.8% for NNRTI, (95% CI, 10.3%–13.5%), 0.2% (95% CI, 0.1%–0.6%) for PI, and 2.5% (95% CI, 1.5%–4.1%) for INSTI. Most of the patients were infected by subtype B (79.8%), while the majority of non-Bs were CRF02_AG (n = 109, 6%). The prevalence of INSTI and PI resistance in Spain during the period 2019–2021 is low, while NRTI resistance is moderate, and NNRTI resistance is the highest. Our results support the use of integrase inhibitors as first-line treatment in Spain. Our findings highlight the importance of ongoing surveillance of TDR to antiretroviral drugs in PLWH particularly with regard to first-line antiretroviral therapy.This work is supported in part by grants from Plan Nacional de I+D+I and Fondo Europeo de Desarrollo Regional-FEDER (www.redes/redes/inicio) (RD16/0025/0040; RD16/0025/0026), Fundacion Progreso y salud, Junta de Andalucia (http://www.juntadeandalucia.es/fundacionprogresoysalud/es) (PI- 0550-2017), FIS PI 18/00819 and FIS PI 22/00882; Centro de Investigación Biomédica en Red (CIBER 21/13/00088). Adolfo de Salazar and Ana Fuentes are supported by “Instituto de Salud Carlos III” (grant number CM20/00016 and CM21/00060, respectively)

Direct long-read RNA sequencing uncovers functional variation affecting transcript production and RNA modifications

The production of multiple transcripts per gene is a process regulated by inherited genetic variants and epitranscriptomic modifications, and plays a prominent role in modulating complex traits and diseases. To simultaneously characterize the effect of genetic variants on transcript abundance and N6-methyladenosine (m6A) modifications, we produced long-read native poly(A) RNA-seq data for 60 genetically different lymphoblastoid cell lines (LCLs) from the 1000 Genomes/Geuvadis project. We identified a high diversity of both annotated (31%) and unannotated (61%) transcripts, with only a small proportion expressed across individuals (35% and 7%, respectively). In a genome-wide genetic analysis on transcripts, we identified 105 trQTLs, of which 76 were not detected as eQTLs using a larger published short-read RNAseq dataset (317 samples). A population wide characterization of m6A methylation DRACH motifs identified an average of 40.1 m6A modifications on 6,222 genes. Genetic association analysis of highly variable modifications from 1,155 genes identified m6A modification quantitative trait loci (m6A-QTLs) for 16 transcripts. Colocalization analysis of trQTL and m6A-QTLs, identified 33 candidate transcripts mediating GWAS traits, with 46.4% of the colocalized trQTLs implicating novel risk transcripts. Overall, the simultaneous characterization of transcripts and post-transcriptional modifications identified genetic effects on transcription often missed when using other sequencing technologies