Detection of a z=0.0515, 0.0522 absorption system in the QSO S4 0248+430 due to an intervening galaxy

In some of the few cases where the line of sight to a Quasi-Stellar Object (QSO) passes near a galaxy, the galaxy redshift is almost identical to an absorption redshift in the spectrum of the QSO. Although these relatively low redshift QSO-galaxy pairs may not be typical of the majority of the narrow heavy-element QSO absorption systems, they provide a direct measure of column densities in the outer parts of galaxies and some limits on the relative abundances of the gas. Observations are presented here of the QSO S4 0248+430 and a nearby anonymous galaxy (Kuhr 1977). The 14 second separation of the line of sight to the QSO (z sub e = 1.316) and the z=0.052 spiral galaxy, (a projected separation of 20 kpc ((h sub o = 50, q sub o = 0)), makes this a particularly suitable pair for probing the extent and content of gas in the galaxy. Low resolution (6A full width half maximum), long slit charge coupled device (CCD) spectra show strong CA II H and K lines in absorption at the redshift of the galaxy (Junkkarinen 1987). Higher resolution spectra showing both Ca II H and K and Na I D1 and D2 in absorption and direct images are reported here

Medical Device Incident Reports by Professional Users in Finland 2014 2021

Publisher Copyright: © 2022 The authors and IOS Press.Medical Device incident reporting is a legal obligation for professional users in Finland. We analyzed all medical device incident reports recorded into the national incident repository from January 2014 to August 2021. Among the total 5,897 records, annual numbers of incident reports varied between 463 and 1,190. Approximately 80% of the medical device incident reports were near misses, 18.7% were person injuries and 1.3% deaths. The number of annual medical device incident reports between hospital districts varied more than expected when related to the population of catchment area. There was a tendency towards lesser reports per population from smaller hospital districts. In conclusion, medical device incident reporting activity of the professional user varied both annually and geographically. A high number of incidents caused person injuries or even death, which arouses safety concerns. A further analysis is required to explore the causes behind our findings.Peer reviewe

Electronic Health Records on the Top of Medical Device Incident Reports

Publisher Copyright: © 2022 European Federation for Medical Informatics (EFMI) and IOS Press.Medical Device incident reporting is a legal obligation for professional users in Finland. We analyzed all medical device incident reports recorded into the national incident repository from January 2014 to August 2021. Almost 30% of the total of 5,897 recorded incidents were caused by top ten devices, of which electronic health records were the most common (332 incidents). High number of incidents caused by electronic health records arouses safety concerns. A further analysis is required to explore the causes of findings.Peer reviewe

DigiBuzz-VTT – Towards digital twin’s concrete commercial exploitation

The DigiBuzz-VTT project, a part of the DigiBuzz common effort, focused on the applications of digital twins in manufacturing industry ecosystems. The DigiBuzz-VTT project had two main focuses, 1) functional digital twins, or simulation-based digital twins, of machines and machine systems and their applications, and 2) the life cycle management of digital twins (the digital part of the twin), emphasising data modelling and data management. These themes were studied from the technical and from the business point of views. The detailed research topics were:• Business opportunities and added value of digital twins for manufacturing industry• Data-based digital twins, use of machine learning for feature recognition• The status of standardisation for the lifecycle data management of digital twins, means for preserving model data• Hybrid modelling with digital twins, combination of experimental and simulation data• The optimisation of the measurement points location, method development• The use of Kalman filters in estimating simulation data correlation with measured data• The status of Industrial Internet of Things (IIoT) for digital twinsThis report summarises the implementation of the DigiBuzz-VTT project and lists the main deliverables of the project. The project produced several scientific articles and research reports, which report the research results in detail

Short-term bone biochemical response to a single bout of high-impact exercise

Bone response to a single bout of exercise can be observed with biochemical markers of bone formation and resorption. The purpose of this study was to examine the response of bone biochemical markers to a single bout of exhaustive high-impact exercise. 15 physically active young subjects volunteered to participate. The subjects performed continuous bilateral jumping with the ankle plantarflexors at 65 % of maximal ground reaction force (GRF) until exhaustion. Loading was characterized by analyzing the GRF recorded for the duration of the exercise. Venous blood samples were taken at baseline, immediately after, 2h and on day 1 and day 2 after the exercise. Procollagen type I amino terminal propeptide (P1NP, marker of bone formation) and carboxyterminal crosslinked telopeptide (CTx, marker of bone resorption) were analyzed from the blood samples. CTx increased significantly (32 %, p = 0.015) two days after the exercise and there was a tendensy towards increase seen in P1NP (p = 0.053) one day after the exercise. A significant positive correlation (r = 0.49 to 0.69, p &le; 0.038) was observed between change in P1NP from baseline to day 1 and exercise variables (maximal slope of acceleration, body weight (BW) adjusted maximal GRF, BW adjusted GRF exercise intensity and osteogenic index). Based on the two biochemical bone turnover markers, it can be concluded that bone turnover is increased in response to a very<br /

Generation of mice with a conditional allele for the p75 NTR neurotrophin receptor gene

The p75 NTR neurotrophin receptor has been implicated in multiple biological and pathological processes. While significant advances have recently been made in understanding the physiologic role of p75 NTR , many details and aspects remain to be determined. This is in part because the two existing knockout mouse models (Exons 3 or 4 deleted, respectively), both display features that defy definitive conclusions. Here we describe the generation of mice that carry a conditional p75 NTR (p75 NTR‐FX ) allele made by flanking Exons 4–6, which encode the transmembrane and all cytoplasmic domains, by loxP sites. To validate this novel conditional allele, both neural crest‐specific p75 NTR /Wnt1‐Cre mutants and conventional p75 NTR null mutants were generated. Both mutants displayed abnormal hind limb reflexes, implying that loss of p75 NTR in neural crest‐derived cells causes a peripheral neuropathy similar to that seen in conventional p75 NTR mutants. This novel conditional p75 NTR allele will offer new opportunities to investigate the role of p75 NTR in specific tissues and cells. genesis 49:862–869, 2011. © 2011 Wiley‐Periodicals, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/88029/1/20747_ftp.pd

Structural and functional characteristics of xenavidin, the first frog avidin from Xenopus tropicalis

<p>Abstract</p> <p>Background</p> <p>Avidins are proteins with extraordinarily high ligand-binding affinity, a property which is used in a wide array of life science applications. Even though useful for biotechnology and nanotechnology, the biological function of avidins is not fully understood. Here we structurally and functionally characterise a novel avidin named xenavidin, which is to our knowledge the first reported avidin from a frog.</p> <p>Results</p> <p>Xenavidin was identified from an EST sequence database for <it>Xenopus tropicalis </it>and produced in insect cells using a baculovirus expression system. The recombinant xenavidin was found to be homotetrameric based on gel filtration analysis. Biacore sensor analysis, fluorescently labelled biotin and radioactive biotin were used to evaluate the biotin-binding properties of xenavidin - it binds biotin with high affinity though less tightly than do chicken avidin and bacterial streptavidin. X-ray crystallography revealed structural conservation around the ligand-binding site, while some of the loop regions have a unique design. The location of structural water molecules at the entrance and/or within the ligand-binding site may have a role in determining the characteristic biotin-binding properties of xenavidin.</p> <p>Conclusion</p> <p>The novel data reported here provide information about the biochemically and structurally important determinants of biotin binding. This information may facilitate the discovery of novel tools for biotechnology.</p

Altered expiratory flow dynamics at peak exercise in adult men with well-controlled type 1 diabetes.

Peer reviewe

Internalization of novel non-viral vector TAT-streptavidin into human cells

BACKGROUND: The cell-penetrating peptide derived from the Human immunodeficiency virus-1 transactivator protein Tat possesses the capacity to promote the effective uptake of various cargo molecules across the plasma membrane in vitro and in vivo. The objective of this study was to characterize the uptake and delivery mechanisms of a novel streptavidin fusion construct, TAT(47–57)-streptavidin (TAT-SA, 60 kD). SA represents a potentially useful TAT-fusion partner due to its ability to perform as a versatile intracellular delivery vector for a wide array of biotinylated molecules or cargoes. RESULTS: By confocal and immunoelectron microscopy the majority of internalized TAT-SA was shown to accumulate in perinuclear vesicles in both cancer and non-cancer cell lines. The uptake studies in living cells with various fluorescent endocytic markers and inhibiting agents suggested that TAT-SA is internalized into cells efficiently, using both clathrin-mediated endocytosis and lipid-raft-mediated macropinocytosis. When endosomal release of TAT-SA was enhanced through the incorporation of a biotinylated, pH-responsive polymer poly(propylacrylic acid) (PPAA), nuclear localization of TAT-SA and TAT-SA bound to biotin was markedly improved. Additionally, no significant cytotoxicity was detected in the TAT-SA constructs. CONCLUSION: This study demonstrates that TAT-SA-PPAA is a potential non-viral vector to be utilized in protein therapeutics to deliver biotinylated molecules both into cytoplasm and nucleus of human cells