265 research outputs found
The Non-Coding Transcriptome of Prostate Cancer: Implications for Clinical Practice
Prostate cancer (PCa) is the most common type of cancer and the second leading cause of cancer-related death in men. Despite extensive research, the molecular mechanisms underlying PCa initiation and progression remain unclear, and there is increasing need of better biomarkers that can distinguish indolent from aggressive and life-threatening disease. With the advent of advanced genomic technologies in the last decade, it became apparent that the human genome encodes tens of thousands non-protein-coding RNAs (ncRNAs) with yet to be discovered function. It is clear now that the majority of ncRNAs exhibit highly specific expression patterns restricted to certain tissues and organs or developmental stages and that the expression of many ncRNAs is altered in disease and cancer, including cancer of the prostate. Such ncRNAs can serve as important biomarkers for PCa diagnosis, prognosis, or prediction of therapy response. In this review, we give an overview of the different types of ncRNAs and their function, describe ncRNAs relevant for the diagnosis and prognosis of PCa, and present emerging new aspects of ncRNA research that may contribute to the future utilization of ncRNAs as clinically useful therapeutic targets
Influence of polymer excluded volume on the phase behavior of colloid-polymer mixtures
We determine the depletion-induced phase-behavior of hard sphere colloids and
interacting polymers by large-scale Monte Carlo simulations using very accurate
coarse-graining techniques. A comparison with standard Asakura-Oosawa model
theories and simulations shows that including excluded volume interactions
between polymers leads to qualitative differences in the phase diagrams. These
effects become increasingly important for larger relative polymer size. Our
simulations results agree quantitatively with recent experiments.Comment: 5 pages, 4 figures submitted to Physical Review Letter
Mental States Are Like Diseases
While Quine’s linguistic behaviorism is well-known, his Kant Lectures contain one of his most detailed discussions of behaviorism in psychology and the philosophy of mind. Quine clarifies the nature of his psychological commitments by arguing for a modest view that is against ‘excessively restrictive’ variants of behaviorism while maintaining ‘a good measure of behaviorist discipline…to keep [our mental] terms under control’. In this paper, I use Quine’s Kant Lectures to reconstruct his position. I distinguish three types of behaviorism in psychology and the philosophy of mind: ontological behaviorism, logical behaviorism, and epistemological behaviorism. I then consider Quine’s perspective on each of these views and argue that he does not fully accept any of them. By combining these perspectives we arrive at Quine’s surprisingly subtle view about behaviorism in psychology
Aniline incorporated silica nanobubbles
We report the synthesis of stearate functionalized nanobubbles of SiO2 with a few aniline
molecules inside, represented as C6H5NH2@SiO2@stearate, exhibiting fluorescence with red-shifted
emission. Stearic acid functionalization allows the materials to be handled just as free molecules, for dissolution,
precipitation, storage etc. The methodology adopted involves adsorption of aniline on the surface of
gold nanoparticles with subsequent growth of a silica shell through monolayers, followed by the selective
removal of the metal core either using sodium cyanide or by a new reaction involving halocarbons. The
material is stable and can be stored for extended periods without loss of fluorescence. Spectroscopic and
voltammetric properties of the system were studied in order to understand the interaction of aniline with
the shell as well as the monolayer, whilst transmission electron microscopy has been used to study the
silica shell
Prevalence of xenotropic murine leukaemia virus-related virus in patients with chronic fatigue syndrome in the Netherlands: retrospective analysis of samples from an established cohort
Objective The presence of the retrovirus xenotropic murine leukaemia virus-related virus (XMRV) has been reported in peripheral blood mononuclear cells of patients with chronic fatigue syndrome. Considering the potentially great medical and social relevance of such a discovery, we investigated whether this finding could be confirmed in an independent European cohort of patients with chronic fatigue syndrome
The defect in the AT-like hamster cell mutants is complemented by mouse chromosome 9 but not by any of the human chromosomes
X-ray-sensitive Chinese hamster V79 cells mutants, V-C4, V-E5 and V-G8, show an abnormal response to X-ray-induced DNA damage. Like ataxia telangiectasia (AT) cells, they display increased cell killing, chromosomal instability and a diminished inhibition of DNA synthesis following ionizing radiation. To localize the defective hamster gene (XRCC8) on the human genome, human chromosomes were introduced into the AT-like hamster mutants, by microcell mediated chromosome transfer. Although, none of the human chromosomes corrected the defect in these mutants, the defect was corrected by a single mouse chromosome, derived from the A9 microcell donor cell line. In four independent X-ray-resistant microcell hybrid clones of V-E5, the presence of the mouse chromosome was determined by fluorescent in situ hybridization, using a mouse cot-1 probe. By PCR analysis with primers specific for different mouse chromosomes and Southern blot analysis with the mouse Ldlr probe, the mouse chromosome 9, was identified in all four X-ray-resistant hybrid clones. Segregation of the mouse chromosome 9 from these hamster-mouse microcell hybrids led to the loss of the regained X-ray-resistance, confirming that mouse chromosome 9 is responsible for complementation of the defect in V-E5 cells. The assignment of the mouse homolog of the ATM gene to mouse chromosome 9, and the presence of this mouse chromosome only in the radioresistant hamster cell hybrids suggest that the hamster AT-like mutants are homologous to AT, although they are not complemented by human chromosome 11
Spectroscopic investigations of detachment on the MAST Upgrade Super-X divertor
We present the first analysis of the atomic and molecular processes at play
during detachment in the MAST-U Super-X divertor using divertor spectroscopy
data. Our analysis indicates detachment in the MAST-U Super-X divertor can be
separated into four sequential phases: First, the ionisation region detaches
from the target at detachment onset leaving a region of increased molecular
densities downstream. The plasma interacts with these molecules, resulting in
molecular ions ( and/or ) that further react
with the plasma leading to Molecular Activated Recombination and Dissociation
(MAR and MAD), which results in excited atoms and significant Balmer line
emission. Second, the MAR region detaches from the target leaving a sub-eV
temperature region downstream. Third, an onset of strong emission from
electron-ion recombination (EIR) ensues. Finally, the electron density decays
near the target, resulting in a density front moving upstream.
The analysis in this paper indicates that plasma-molecule interactions have a
larger impact than previously reported and play a critical role in the
intensity and interpretation of hydrogen atomic line emission characteristics
on MAST-U. Furthermore, we find that the Fulcher band emission profile in the
divertor can be used as a proxy for the ionisation region and may also be
employed as a plasma temperature diagnostic for improving the separation of
hydrogenic emission arising from electron-impact excitation and that from
plasma-molecular interactions.
We provide evidences for the presence of low electron temperatures (
eV) during detachment phases III-IV based on quantitative spectroscopy
analysis, a Boltzmann relation of the high-n Balmer line transitions together
with an analysis of the brightness of high-n Balmer lines
- …