Diseño y Análisis de viabilidad económica de recuperación energética en una red de riego a presión para el Sector Betíes de la CC.RR. de La Romana

[ES] En este trabajo, se diseñará una red de distribución de riego. La red en cuestión, se encuentra en el término municipal de Novelda y pertenece a la “Comunidad de Regantes de La Romana”, concretamente al sector “Beties”. Dicho sector, proporciona servicio a 603,77 ha, a partir de una balsa con una capacidad de 450.000 m3 y con un total de 93 hidrantes. El dimensionado se realizará mediante un diseño a la demanda, basado en la metodología de Clement y aplicando criterios validados en redes reales de riego. También se llevará a cabo, el dimensionado y diseño de los anclajes de las tuberías, así como otros elementos e instalaciones necesarias. Se realizará también un análisis de viabilidad económica de recuperación energética, mediante la instalación de sistemas basados en Pumps As Turbines (PATs), determinando a su vez la energía a recuperar y su mejor ubicación.[EN] In this work, an irrigation distribution network will be designed. This network is located in “Novelda” and belongs to the “Comunidad de Regantes de La Romana”, in particular, the “Beties” sector. This sector provides service to 603.77 ha, from 450.000 m3 resrvoir and with 93 hydrants. The design and sizing of the pipe anchors will also be carried out, as well as other necessary elements and facilities. An analysis of the economic viability of the recoverable energy is also performed, by installating of systems based on Pumps As Turbines (PATs), setting at the same time the energy to be recovered and its best location.Sirera Vera, M. (2021). Diseño y Análisis de viabilidad económica de recuperación energética en una red de riego a presión para el Sector Betíes de la CC.RR. de La Romana. Universitat Politècnica de València. http://hdl.handle.net/10251/179101TFG

Immunohistochemical expression of glucose transporter 1 in keratin-producing odontogenic cysts

Background: Keratin-producing odontogenic cysts (KPOCs) are a group of cystic lesions that are often aggressive, with high rates of recurrence and multifocality. KPOCs included orthokeratinised odontogenic cyst (OOC) and parakeratotic odontogenic cysts, which are now considered true tumours denominated keratocystic odontogenic tumours (KCOTs). GLUT1 is a protein transporter that is involved in the active uptake of glucose across cell membranes and that is overexpressed in tumours in close correlation with the proliferation rate and positron emission tomography (PET) imaging results. Methods: A series of 58 keratin-producing odontogenic cysts was evaluated histologically and immunohistochemically in terms of GLUT1 expression. Different data were correlated using the beta regression model in relation to histological type and immunohistochemical expression of GLUT1, which was quantified using two different morphological methods. Results: KPOC cases comprised 12 OOCs and 46 KCOTs, the latter corresponding to 6 syndromic and 40 sporadic KCOTs. GLUT1 expression was very low in OOC cases compared with KCOT cases, with statistical significant differences when quantification was considered. Different GLUT1 localisation patterns were revealed by immunostaining, with the parabasal cells showing higher reactivity in KCOTs. However, among KCOTs cases, GLUT1 expression was unable to establish differences between syndromic and sporadic cases. Conclusions: GLUT1 expression differentiated between OOC and KCOT cases, with significantly higher expression in KCOTs, but did not differentiate between syndromic and sporadic KCOT cases. However, given the structural characteristics of KCOTs, we hypothesised that PET imaging methodology is probably not a useful diagnostic tool for KCOTs. Further studies of GLUT1 expression and PET examination in KCOT series are needed to confirm this last hypothesis. Keywords: Glucose transporter protein, Immunohistochemistry, Keratin-producing odontogenic cyst, Keratocystic odontogenic tumour, Orthokeratinised odontogenic cyst, Positron emission tomograph

Synovial cysts of the temporomandibular joint: an immunohistochemical characterization and literature review.

Synovial cysts of the temporomandibular joint (TMJ) are very rare, and to date, only 12 cases of a synovial cyst in the TMJ region have been reported in the literature. In this paper, we present the clinicopathological and immunohistochemical characteristics of one such lesion affecting a 48-year-old woman, presented with a mass in the left preauricular region.We describe the usefulness of immunohistochemical analysis for recognizing the synovial lining, which allowed for clear differentiation between ganglion and synovial cysts. Immunohistochemical analyses can be used to diagnose synovial cysts with certainty; however, using at least two markers is advisable to distinguish the two existing synovial cell subtypes. Our findings indicate that synovial cysts of TMJ possess an internal lining dominated by type B (fibroblast-like) synoviocytes

Orthokeratinized odontogenic cysts: a Spanish tertiary care center study based on HPV DNA detection

The role of human papillomavirus (HPV) in orthokeratinized odontogenic cysts (OOCs) has rarely been studied. The objective is to describe the clinicopathological findings in a series of OOCs from a Spanish population that were investigated in relation to the possible presence of HPV. Methods A clinicopathological retrospective analysis followed by a molecular analysis of 28 high- and low-risk HPV genotypes was performed in OOC samples of patients seen during the last 15-years in a Spanish tertiary care center. Results Of 115 odontogenic cysts with keratinization, 16 cases of OOCs were confirmed and evaluated. OOCs occurred predominantly in the mandible of males (mean age 36.06 ± 13.16 years). Swelling of the jaw followed by pain were the most common clinical symptoms, and 56.5% of the OOC cases were associated with an unerupted tooth. After a mean post-cystectomy follow-up of 3.8 years, only one recurrent case was observed, resulting in a verrucous cystic lesion that was considered premalignant after immunohistological examination. DNA extraction was successful from 14 of the 16 OOC cases. None of the primary OCCs or the single recurrent OOC were positive for HPV in the molecular analysis. Conclusions OOCs show a very limited potential for recurrence. Our results suggest that neither high- or low-risk HPV subtypes are likely to play a role in the etiology or neoplastic transformation of OOC, at least in the Spanish population

Tumor odontogenico adenomatoide folicular: estudio inmunohistoquimico

El tumor odontogénico adenomatoide (TOA) es una infrecuente lesión odontogénica benigna, que aparece en pacientes jóvenes, generalmente mujeres en la segunda década de la vida, a menudo como una lesión radiolúcida de aspecto quístico unilocular, en asociación a un diente, usualmente canino, no erupcionado. A pesar de haberse denominado también adenoameloblastoma o tumor ameloblástico adenomatoide, el TOA es una lesión benigna con una muy baja tendencia a la recidiva, mostrando una morfología muy peculiar (apariencia basaloide con estructuras glanduliformes, calcificaciones esferulares, presencia de material amiloide) que facilitan su reconocimiento histológico. Se presenta un análisis clínico-patológico de un TOA de tipo folicular de maxilar inferior, en asociación a la inclusión de un canino inferior izquierdo, en una paciente pediátrica de 9 años. El estudio inmunohistoquímico realizado muestra algunos datos previamente no referidos. A pesar de existir distintos tipos celulares en el TOA se observo una universal inmunorreactividad para p63, demostrando el carácter basal de los distintos elementos que lo constituyen. En concordancia con su benignidad y con su baja tasa de recidiva existe una escasa actividad proliferativa (2-3% de núcleos marcados por el antígeno Ki-67), estando la proliferación reducida a pequeños nódulos de células epiteliales (AE1-3 +) de núcleos elongados o fusiformes; de otra parte la mayor incidencia en pacientes de sexo femenino no puede ser explicada en base a la existencia de una hormonodependecia tumoral dada la ausencia de expresión de receptores hormonales (RE y RPg).Adenomatoid odontogenic tumor (AOT) is an uncommon benign odontogenic lesion that affects young patients, with female predominance, mainly in second decade, showing a radiolucent unilocular image associated with an unerupted tooth, usually a canine. In spite of previous and confusing denominations, such as adenoameloblastoma or adenomatoid ameloblastic tumor, AOT is a benign tumor with a very low rate of recurrence, that show a peculiar morphological picture (basaloid appearance with glandular-like structures, calcifying areas, and amiloid-like material) that allow its histopathological recognition. We present a clinicopathological analysis of a case of follicular AOT affecting the mandible in a 9 years-old female patient associated with unerupted lower left canine. Immunohistochemical study showed some data previously unrecognised. All cellular types that composed AOT showed nuclear positivity for p63 indicating a basal characterization in the different cellular components. According to its benign character and low potential for recurrence, AOT revealed a scant proliferative activity (2-3% nuclei showed Ki-67 positivity) limited to some epithelial nodules (AE1-3 +) of fusiform appearance. Absence of reactivity for hormonal receptors (RE and RPg) excluded a possible hormonodependence in AOT that could explain the observed female predominance

Follicular adenomatoid odontogenic tumor : immunohistochemical study

Adenomatoid odontogenic tumor (AOT) is an uncommon benign odontogenic lesion that affects young patients, with female predominance, mainly in second decade, showing a radiolucent unilocular image associated with an unerupted tooth, usually a canine. In spite of previous and confusing denominations, such as adenoameloblastoma or adenomatoid ameloblastic tumor, AOT is a benign tumor with a very low rate of recurrence, that show a peculiar morphological picture (basaloid appearance with glandular-like structures, calcifying areas, and amiloid-like material) that allow its histopathological recognition. We present a clinicopathological analysis of a case of follicular AOT affecting the mandible in a 9 years-old female patient associated with unerupted lower left canine. Immunohistochemical study showed some data previously unrecognised. All cellular types that composed AOT showed nuclear positivity for p63 indicating a basal characterization in the different cellular components. According to its benign character and low potential for recurrence, AOT revealed a scant proliferative activity (2-3% nuclei showed Ki-67 positivity) limited to some epithelial nodules (AE1-3 +) of fusiform appearance. Absence of reactivity for hormonal receptors (RE and RPg) excluded a possible hormonodependence in AOT that could explain the observed female predominance

In search for the role of thermospermine synthase gene in poplar vascular development

This work is supported by the FCT project PTDC/AGR-GPL/098369/2008 and FCT PhD grant SFRH/BD/30074/2006 (A.M.).Peer Reviewe

Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions

[EN] Comparison of the proteins of thermophilic, mesophilic, and psychrophilic prokaryotes has revealed several features characteristic to proteins adapted to high temperatures, which increase their thermostability. These characteristics include a profusion of disulfide bonds, salt bridges, hydrogen bonds, and hydrophobic interactions, and a depletion in intrinsically disordered regions. It is unclear, however, whether such differences can also be observed in eukaryotic proteins or when comparing proteins that are adapted to temperatures that are more subtly different. When an organism is exposed to high temperatures, a subset of its proteins is overexpressed (heat-induced proteins), whereas others are either repressed (heat-repressed proteins) or remain unaffected. Here, we determine the expression levels of all genes in the eukaryotic model system Arabidopsis thaliana at 22 and 37 degrees C, and compare both the amino acid compositions and levels of intrinsic disorder of heat-induced and heat-repressed proteins. We show that, compared to heat-repressed proteins, heat-induced proteins are enriched in electrostatically charged amino acids and depleted in polar amino acids, mirroring thermophile proteins. However, in contrast with thermophile proteins, heat-induced proteins are enriched in intrinsically disordered regions, and depleted in hydrophobic amino acids. Our results indicate that temperature adaptation at the level of amino acid composition and intrinsic disorder can be observed not only in proteins of thermophilic organisms, but also in eukaryotic heat-induced proteins; the underlying adaptation pathways, however, are similar but not the same.D.A.-P. and F.F. were supported by funds from the University of Nevada, Reno, and by pilot grants from Nevada INBRE (P20GM103440) and the Smooth Muscle Plasticity COBRE from the University of Nevada, Reno (5P30GM110767-04), both funded by the National Institute of General Medical Sciences (National Institutes of Health). M.X.R.-G. and M.A.F. were supported by grants from Science Foundation Ireland (12/IP/1637) and the Spanish Ministerio de Economia y Competitividad, Spain (MINECO-FEDER; BFU201236346 and BFU2015-66073-P) to MAF. MXRG was supported by a JAE DOC fellowship from the MINECO, Spain. F.V.-S. and M.A.P.-A. were supported by grant BIO2014-55946-P from MINECO-FEDER.Alvarez-Ponce, D.; Ruiz-González, M.; Vera Sirera, FJ.; Feyertag, F.; Perez Amador, MA.; Fares Riaño, MA. (2018). Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions. International Journal of Molecular Sciences. 19(8). https://doi.org/10.3390/ijms19082276S198Karshikoff, A., & Ladenstein, R. (2001). Ion pairs and the thermotolerance of proteins from hyperthermophiles: a ‘traffic rule’ for hot roads. Trends in Biochemical Sciences, 26(9), 550-557. doi:10.1016/s0968-0004(01)01918-1Strop, P., & Mayo, S. L. (2000). Contribution of Surface Salt Bridges to Protein Stability†,‡. Biochemistry, 39(6), 1251-1255. doi:10.1021/bi992257jPERUTZ, M. F., & RAIDT, H. (1975). Stereochemical basis of heat stability in bacterial ferredoxins and in haemoglobin A2. Nature, 255(5505), 256-259. doi:10.1038/255256a0Argos, P., Rossmann, M. G., Grau, U. M., Zuber, H., Frank, G., & Tratschin, J. D. (1979). Thermal stability and protein structure. Biochemistry, 18(25), 5698-5703. doi:10.1021/bi00592a028Beeby, M., O’Connor, B. D., Ryttersgaard, C., Boutz, D. R., Perry, L. J., & Yeates, T. O. (2005). The Genomics of Disulfide Bonding and Protein Stabilization in Thermophiles. PLoS Biology, 3(9), e309. doi:10.1371/journal.pbio.0030309Haney, P. J., Badger, J. H., Buldak, G. L., Reich, C. I., Woese, C. R., & Olsen, G. J. (1999). Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species. Proceedings of the National Academy of Sciences, 96(7), 3578-3583. doi:10.1073/pnas.96.7.3578Kreil, D. P. (2001). Identification of thermophilic species by the amino acid compositions deduced from their genomes. Nucleic Acids Research, 29(7), 1608-1615. doi:10.1093/nar/29.7.1608Tekaia, F., Yeramian, E., & Dujon, B. (2002). Amino acid composition of genomes, lifestyles of organisms, and evolutionary trends: a global picture with correspondence analysis. Gene, 297(1-2), 51-60. doi:10.1016/s0378-1119(02)00871-5Zeldovich, K. B., Berezovsky, I. N., & Shakhnovich, E. I. (2007). Protein and DNA Sequence Determinants of Thermophilic Adaptation. PLoS Computational Biology, 3(1), e5. doi:10.1371/journal.pcbi.0030005Chakravarty, S., & Varadarajan, R. (2000). Elucidation of determinants of protein stability through genome sequence analysis. FEBS Letters, 470(1), 65-69. doi:10.1016/s0014-5793(00)01267-9Cambillau, C., & Claverie, J.-M. (2000). Structural and Genomic Correlates of Hyperthermostability. Journal of Biological Chemistry, 275(42), 32383-32386. doi:10.1074/jbc.c000497200Burra, P. V., Kalmar, L., & Tompa, P. (2010). Reduction in Structural Disorder and Functional Complexity in the Thermal Adaptation of Prokaryotes. PLoS ONE, 5(8), e12069. doi:10.1371/journal.pone.0012069Wang, J., Yang, Y., Cao, Z., Li, Z., Zhao, H., & Zhou, Y. (2013). The Role of Semidisorder in Temperature Adaptation of Bacterial FlgM Proteins. Biophysical Journal, 105(11), 2598-2605. doi:10.1016/j.bpj.2013.10.026Vicedo, E., Schlessinger, A., & Rost, B. (2015). Environmental Pressure May Change the Composition Protein Disorder in Prokaryotes. PLOS ONE, 10(8), e0133990. doi:10.1371/journal.pone.0133990Galea, C. A., High, A. A., Obenauer, J. C., Mishra, A., Park, C.-G., Punta, M., … Kriwacki, R. W. (2009). Large-Scale Analysis of Thermostable, Mammalian Proteins Provides Insights into the Intrinsically Disordered Proteome. Journal of Proteome Research, 8(1), 211-226. doi:10.1021/pr800308vTsvetkov, P., Myers, N., Moscovitz, O., Sharon, M., Prilusky, J., & Shaul, Y. (2012). Thermo-resistant intrinsically disordered proteins are efficient 20S proteasome substrates. Mol. BioSyst., 8(1), 368-373. doi:10.1039/c1mb05283gGalea, C. A., Nourse, A., Wang, Y., Sivakolundu, S. G., Heller, W. T., & Kriwacki, R. W. (2008). Role of Intrinsic Flexibility in Signal Transduction Mediated by the Cell Cycle Regulator, p27Kip1. Journal of Molecular Biology, 376(3), 827-838. doi:10.1016/j.jmb.2007.12.016Van Noort, V., Bradatsch, B., Arumugam, M., Amlacher, S., Bange, G., Creevey, C., … Bork, P. (2013). Consistent mutational paths predict eukaryotic thermostability. BMC Evolutionary Biology, 13(1), 7. doi:10.1186/1471-2148-13-7Wang, G.-Z., & Lercher, M. J. (2010). Amino acid composition in endothermic vertebrates is biased in the same direction as in thermophilic prokaryotes. BMC Evolutionary Biology, 10(1), 263. doi:10.1186/1471-2148-10-263Windisch, H. S., Lucassen, M., & Frickenhaus, S. (2012). Evolutionary force in confamiliar marine vertebrates of different temperature realms: adaptive trends in zoarcid fish transcriptomes. BMC Genomics, 13(1), 549. doi:10.1186/1471-2164-13-549Albanèse, V., Yam, A. Y.-W., Baughman, J., Parnot, C., & Frydman, J. (2006). Systems Analyses Reveal Two Chaperone Networks with Distinct Functions in Eukaryotic Cells. Cell, 124(1), 75-88. doi:10.1016/j.cell.2005.11.039Berry, J., & Bjorkman, O. (1980). Photosynthetic Response and Adaptation to Temperature in Higher Plants. Annual Review of Plant Physiology, 31(1), 491-543. doi:10.1146/annurev.pp.31.060180.002423Sueoka, N. (1961). CORRELATION BETWEEN BASE COMPOSITION OF DEOXYRIBONUCLEIC ACID AND AMINO ACID COMPOSITION OF PROTEIN. Proceedings of the National Academy of Sciences, 47(8), 1141-1149. doi:10.1073/pnas.47.8.1141Cherry, J. L. (2009). Highly Expressed and Slowly Evolving Proteins Share Compositional Properties with Thermophilic Proteins. Molecular Biology and Evolution, 27(3), 735-741. doi:10.1093/molbev/msp270The amino acid composition is different between the cytoplasmic and extracellular sides in membrane proteins. (1992). FEBS Letters, 303(2-3), 141-146. doi:10.1016/0014-5793(92)80506-cNakashima, H., & Nishikawa, K. (1994). Discrimination of Intracellular and Extracellular Proteins Using Amino Acid Composition and Residue-pair Frequencies. Journal of Molecular Biology, 238(1), 54-61. doi:10.1006/jmbi.1994.1267Dosztanyi, Z., Csizmok, V., Tompa, P., & Simon, I. (2005). IUPred: web server for the prediction of intrinsically unstructured regions of proteins based on estimated energy content. Bioinformatics, 21(16), 3433-3434. doi:10.1093/bioinformatics/bti541Peng, Z., Uversky, V. N., & Kurgan, L. (2016). Genes encoding intrinsic disorder in Eukaryota have high GC content. Intrinsically Disordered Proteins, 4(1), e1262225. doi:10.1080/21690707.2016.1262225Yruela, I., & Contreras-Moreira, B. (2013). Genetic recombination is associated with intrinsic disorder in plant proteomes. BMC Genomics, 14(1), 772. doi:10.1186/1471-2164-14-772Paliy, O., Gargac, S. M., Cheng, Y., Uversky, V. N., & Dunker, A. K. (2008). Protein Disorder Is Positively Correlated with Gene Expression inEscherichia coli. Journal of Proteome Research, 7(6), 2234-2245. doi:10.1021/pr800055rSingh, G. P., & Dash, D. (2008). How expression level influences the disorderness of proteins. Biochemical and Biophysical Research Communications, 371(3), 401-404. doi:10.1016/j.bbrc.2008.04.072Yang, J.-R., Liao, B.-Y., Zhuang, S.-M., & Zhang, J. (2012). Protein misinteraction avoidance causes highly expressed proteins to evolve slowly. Proceedings of the National Academy of Sciences, 109(14), E831-E840. doi:10.1073/pnas.1117408109Hendsch, Z. S., & Tidor, B. (1994). Do salt bridges stabilize proteins? A continuum electrostatic analysis. Protein Science, 3(2), 211-226. doi:10.1002/pro.5560030206Zhou, X.-X., Wang, Y.-B., Pan, Y.-J., & Li, W.-F. (2007). Differences in amino acids composition and coupling patterns between mesophilic and thermophilic proteins. Amino Acids, 34(1), 25-33. doi:10.1007/s00726-007-0589-xCatanzano, F., Barone, G., Graziano, G., & Capasso, S. (1997). Thermodynamic analysis of the effect of selective monodeamidation at asparagine 67 in ribonuclease A. Protein Science, 6(8), 1682-1693. doi:10.1002/pro.5560060808Charlesworth, B. (2009). Effective population size and patterns of molecular evolution and variation. Nature Reviews Genetics, 10(3), 195-205. doi:10.1038/nrg2526Bolser, D., Staines, D. M., Pritchard, E., & Kersey, P. (2016). Ensembl Plants: Integrating Tools for Visualizing, Mining, and Analyzing Plant Genomics Data. Methods in Molecular Biology, 115-140. doi:10.1007/978-1-4939-3167-5_6Kasprzyk, A. (2003). EnsMart: A Generic System for Fast and Flexible Access to Biological Data. Genome Research, 14(1), 160-169. doi:10.1101/gr.1645104Hooper, C. M., Castleden, I. R., Tanz, S. K., Aryamanesh, N., & Millar, A. H. (2016). SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations. Nucleic Acids Research, 45(D1), D1064-D1074. doi:10.1093/nar/gkw1041R: A language and environment for statistical computing. R Foundation for Statistical Computinghttp://www.R-project.org/Kim, S. (2015). ppcor: An R Package for a Fast Calculation to Semi-partial Correlation Coefficients. Communications for Statistical Applications and Methods, 22(6), 665-674. doi:10.5351/csam.2015.22.6.66

Molecular program of senescence in dry and fleshy fruit

[EN] Fruits of angiosperms can be divided into dry and fleshy fruits, depending on their dispersal strategies. Despite their apparently different developmental programmes, researchers have attempted to compare dry and fleshy fruits to establish analogies of the distinct biochemical and physiological processes that occur. But what are the common and specific phenomena in both biological strategies? Is valve dehiscence and senescence of dry fruits comparable to final ripening of fleshy fruits, when seeds become mature and fruits are competent for seed dispersal, or to over-ripening when advanced senescence occurs? We briefly review current knowledge on dry and fleshy fruit development, which has been extensively reported recently, and is the topic of this special issue. We compare the processes taking place in Arabidopsis (dry) and tomato (fleshy) fruit during final development steps using transcriptome data to establish possible analogies. Interestingly, the transcriptomic programme of Arabidopsis silique shares little similarity in gene number to tomato fruit ripening or over-ripening. In contrast, the biological processes carried out by these common genes from ripening and over-ripening programmes are similar, as most biological processes are shared during both programmes. On the other hand, several biological terms are specific of Arabidopsis and tomato ripening, including senescence, but little or no specific processes occur during Arabidopsis and tomato over-ripening. These suggest a closer analogy between silique senescence and ripening than over-ripening, but a major common biological programme between Arabidopsis silique senescence and the last steps of tomato development, irrespective of its distinction between ripening and over-ripening.We wish to thank Dr J. Carbonell for critically reading the manuscript. We also thank Clara Pons for the preliminary tomato microarray data mining. Our work has been supported by grants BIO2008-01039 and BIO2011-26302 from the Spanish Ministry of Science and Innovation and ACOMP/2010/079, ACOMP/2011/287, and ACOMP/2013/048 from the Generalitat Valenciana.Gómez Jiménez, MD.; Vera Sirera, FJ.; Perez Amador, MA. (2014). Molecular program of senescence in dry and fleshy fruit. Journal of Experimental Botany. 65(16):4515-4526. https://doi.org/10.1093/jxb/eru093S45154526651

Arabidopsis copper transport protein COPT2 participates in the crosstalk between iron deficiency responses and low phosphate signaling

[EN] Copper and iron are essential micronutrients for most living organisms because they participate as cofactors in biological processes, including respiration, photosynthesis, and oxidative stress protection. In many eukaryotic organisms, including yeast (Saccharomyces cerevisiae) and mammals, copper and iron homeostases are highly interconnected; yet, such interdependence is not well established in higher plants. Here, we propose that COPT2, a high-affinity copper transport protein, functions under copper and iron deficiencies in Arabidopsis (Arabidopsis thaliana). COPT2 is a plasma membrane protein that functions in copper acquisition and distribution. Characterization of the COPT2 expression pattern indicates a synergic response to copper and iron limitation in roots. We characterized a knockout of COPT2, copt2-1, that leads to increased resistance to simultaneous copper and iron deficiencies, measured as reduced leaf chlorosis and improved maintenance of the photosynthetic apparatus. We propose that COPT2 could play a dual role under iron deficiency. First, COPT2 participates in the attenuation of copper deficiency responses driven by iron limitation, possibly to minimize further iron consumption. Second, global expression analyses of copt2-1 versus wild-type Arabidopsis plants indicate that low-phosphate responses increase in the mutant. These results open up new biotechnological approaches to fight iron deficiency in crops.This work was supported by the Spanish Ministry of Economy and Competitiveness (grant nos. BIO2011-24848 and CSD2007-00057 to L. P. and predoctoral Formacion Personal Investigador fellowships to A. P.-G and A. G.-M.) and by the European Regional Developmental Fund of the European Union.Perea García, A.; Garcia Molina, A.; Andres-Colas, N.; Vera Sirera, FJ.; Perez Amador, MA.; Puig, S.; Penarrubia, L. (2013). Arabidopsis copper transport protein COPT2 participates in the crosstalk between iron deficiency responses and low phosphate signaling. Plant Physiology. 162(1):180-194. https://doi.org/10.1104/pp.112.212407S180194162