Influence of the hydrological changes on the phytoplantkton structure and dynamics in a subtropical wetland-lake system

The hydroperiod-phytoplankton response in wetlands-lake coastal system is proposed in this study. The samples were taken monthly in three subsystems (North, transition and South) of Taim’s Hydrologic System (THS), since March to December in 2001. Only five species, two diatoms (Aulacoseira granulata and Synedra acus) and three cyanobacteria (Aphanocapsa delicatissima, Cylindrospermopsis raciborski and Planktolyngbya contorta) were related to water dynamics from 333 phytoplankton species identified to the THS. Diatoms species showed high densities in Mangueira Lake (north zone) during spring, when the highest wind velocity and water level were registered. On the other hand, cyanobacteria species were abundant in Mangueira Lake during calm periods and low water level. In the subsystems North and transition, none spatial phytoplankton patterns were identified as observed in South subsystem. Subsystems North and transition are constituted by small lakes where the wind force is not strong enough to drive hydrodynamics process. Therefore, in Mangueira Lake (South subsystem from THS) the hydrodynamic was the force which drove phytoplankton structure.A resposta do fitoplâncton ao hidroperíodo em um sistema costeiro lagoa-banhado é proposta neste estudo. As amostras foram tomadas mensalmente em três subsistemas (norte, transição e sul) do Sistema Hidrológico do Taim (SHT), de março a dezembro de 2001. Das 333 espécies fitoplanctônicas identificadas para o SHT somente cinco espécies, duas diatomáceas (Aulacoseira granulata e Synedra acus ) e três cianobactérias (Aphanocapsa delicatissima, Cylindrospermopsis raciborski e Planktolyngbya contorta) estiveram relacionadas com a dinâmica da água. Espécies de diatomáceas mostraram elevadas densidades na Lagoa Mangueira (zona norte) durante a primavera, quando os valores mais altos de velocidade do vento e nível d’água foram registrados. Por outro lado, espécies de cianobactérias foram abundantes na Lagoa Mangueira durante períodos calmos e baixo nível d’água. Nos subsistemas norte e de transição um padrão espacial não foi identificado para o fitoplâncton como observado no subsistema sul. Subsistemas norte e de transição são formados por pequenas lagoas onde a força do vento não é forte o suficiente para afetar os processos hidrodinâmicos. Portanto, na Lagoa Mangueira (subsistema sul do SHT) a hidrodinâmica foi a força que regula a estrutura do fitoplâncton

Educación en el trabajo de los profisionales de la salud en hospitales públicos

Estudo exploratório-descrito cujos objetivos foram identificar, caracterizar e analisar as atividades educativas desenvolvidas com profissionais de saúde, à luz das concepções de integralidade, trabalho em equipe e educação permanente. A coleta de dados ocorreu em três hospitais do Município de São Paulo, através de entrevista dirigida a informantes-chave, representantes de todas as categorias profissionais. As entrevistas foram gravadas e transcritas, as informações sistematizadas em categorias operacionais e armazenadas em banco de dados. Os resultados mostram que predominaram atividades educativas voltadas às ações de recuperação da saúde, com participação de profissionais de áreas específicas, revelando um distanciamento da concepção de integralidade e de trabalho em equipe. Concluiu-se que as ações de educação dos profissionais de saúde reiteram o modelo clínico de assistência individual, com fragmentação das ações. Recomenda-se que elas sejam repensadas como estratégia integradora de saberes, capazes de promover a integralidade na atenção hospitalar.The objectives of this descriptive exploratory study were to identify, characterize and analyze educational activities developed with health professionals based on integrality, teamwork and permanent education. Data collection was carried out in three hospitals of the city of São Paulo, by means of key-informant interviews, representing all the professional categories. Interviews were recorded and transcribed, information was systematized into operational categories and storage in data bank. The results showed that educational activities aiming actions to recover health with the assistance of professionals of specific areas, revealed a distance from the integrality conception and team work. It was concluded that actions aiming education of health professionals emphasizes the individual clinical model, with fragmented actions of professional categories. So, we suggest reconsidering the integrated strategies of knowledge to promote integrality of hospital attention.Estudio exploratorio-descriptivo cuyos objetivos fueron identificar, caracterizar y analisar las actividades educativas desarrolladas con profisionales de la salud, a la luz de concepciones de integralidad, trabajo en equipo y educación permanente.La colecta de datos ocurrió en tres hospitales del Municipio de São Paulo, a través de entrevista dirigida a informantes-clave, representantes de todas las categorías profisionales. Las entrevistas fueron grabadas y transcritas, las informaciones sistematizadas en categorías operacionales y almacenadas en banco de datos. Los resultados muestran que predominaron las actividades educativas hacia acciones de recuperación de la salud, con participación de profisionales de áreas específicas, revelando un distanciamiento de la concepción de integralidad y de trabajo en equipo. Concluyóse que las acciones de educación de los profisionales de salud reiteran el modelo clínico individual, con fragmentación de las acciones en las categorías profisionales. Estas acciones deberían ser repensadas como estrategia integradora de saberes, capazes de promover la integralidad en la atención hospitalaria

Educación en el trabajo de los profisionales de la salud en hospitales públicos

The objectives of this descriptive exploratory study were to identify, characterize and analyze educational activities developed with health professionals based on integrality, teamwork and permanent education. Data collection was carried out in three hospitals of the city of São Paulo, by means of key-informant interviews, representing all the professional categories. Interviews were recorded and transcribed, information was systematized into operational categories and storage in data bank. The results showed that educational activities aiming actions to recover health with the assistance of professionals of specific areas, revealed a distance from the integrality conception and team work. It was concluded that actions aiming education of health professionals emphasizes the individual clinical model, with fragmented actions of professional categories. So, we suggest reconsidering the integrated strategies of knowledge to promote integrality of hospital attention.Estudo exploratório-descrito cujos objetivos foram identificar, caracterizar e analisar as atividades educativas desenvolvidas com profissionais de saúde, à luz das concepções de integralidade, trabalho em equipe e educação permanente. A coleta de dados ocorreu em três hospitais do Município de São Paulo, através de entrevista dirigida a informantes-chave, representantes de todas as categorias profissionais. As entrevistas foram gravadas e transcritas, as informações sistematizadas em categorias operacionais e armazenadas em banco de dados. Os resultados mostram que predominaram atividades educativas voltadas às ações de recuperação da saúde, com participação de profissionais de áreas específicas, revelando um distanciamento da concepção de integralidade e de trabalho em equipe. Concluiu-se que as ações de educação dos profissionais de saúde reiteram o modelo clínico de assistência individual, com fragmentação das ações. Recomenda-se que elas sejam repensadas como estratégia integradora de saberes, capazes de promover a integralidade na atenção hospitalar.Estudio exploratorio-descriptivo cuyos objetivos fueron identificar, caracterizar y analisar las actividades educativas desarrolladas con profisionales de la salud, a la luz de concepciones de integralidad, trabajo en equipo y educación permanente.La colecta de datos ocurrió en tres hospitales del Municipio de São Paulo, a través de entrevista dirigida a informantes-clave, representantes de todas las categorías profisionales. Las entrevistas fueron grabadas y transcritas, las informaciones sistematizadas en categorías operacionales y almacenadas en banco de datos. Los resultados muestran que predominaron las actividades educativas hacia acciones de recuperación de la salud, con participación de profisionales de áreas específicas, revelando un distanciamiento de la concepción de integralidad y de trabajo en equipo. Concluyóse que las acciones de educación de los profisionales de salud reiteran el modelo clínico individual, con fragmentación de las acciones en las categorías profisionales. Estas acciones deberían ser repensadas como estrategia integradora de saberes, capazes de promover la integralidad en la atención hospitalaria

L-arginine abolishes the hypothalamic serotonergic activation induced by central interleukin-1 beta administration to normal rats

IL-1 beta-induced anorexia may depend on interactions of the cytokine with neuropeptides and neurotransmitters of the central nervous system control of energy balance and serotonin is likely to be one catabolic mediator targeted by IL-1 beta. in the complex interplay involved in feeding modulation, nitric oxide has been ascribed a stimulatory action, which could be of significance in counteracting IL-1 beta effects.The present study aims to explore the participation of the nitric oxide and the serotonin systems on the central mechanisms induced by IL-1 beta and the relevance of their putative interactions to IL-1 beta hypophagia in normal rats. Serotonin levels were determined in microdialysates of the ventromedial hypothalamus after a single intracerebroventricular injection of 10 ng of IL-1 beta, with or without the pre-injection of 20 mu g of the nitric oxide precursor L-arginine. IL-1 beta significantly stimulated hypothalamic serotonin extracellular levels, with a peak variation of 130 +/-37% above baseline. IL-1 beta also reduced the 4-h and the 24-h food intakes (by 23% and 58%, respectively). the IL-1 beta-induced serotonergic activation was abolished by the pre-injection of L-arginine while the hypophagic effect was unaffected.The data showed that one central effect of IL-1 beta is serotonergic stimulation in the ventromedial hypothalamus, an action inhibited by nitric oxide activity. It is suggested that, although serotonin participates in IL-1 beta anorexia, other mechanisms recruited by IL-1 beta in normal rats are able to override the absence of the serotonergic hypophagic influence.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Physiol, BR-04023060 São Paulo, BrazilUniversidade Federal de São Paulo, Div Appl Stat, BR-04023060 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, BR-04023060 São Paulo, BrazilUniversidade Federal de São Paulo, Div Appl Stat, BR-04023060 São Paulo, BrazilWeb of Scienc

"Já que tu fazes psicologia, então..." : reflexões acerca da concepção cotidiana da psicologia

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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly