Effect of fertilization on the contents of macronutrients and chlorine in tobacco leaves cv. flue-cured (Nicotiana tabacum L.) in two municipalities in Huila, Colombia

In Colombia, there is little information on tobacco crops and the effect of fertilization on the nutrient content of leaves. The aim of this study was to determine the contents of mineral nutrients in tobacco (Nicotiana tabacum L. cv. flue-cured) plants subjected to different fertilizer rates and sources in two municipalities in the Huila Province (Colombia). Six foliar analyses, with three measurements from vegetative growth to f lowering and three measurements during harvest, were carried out. Seven treatments, including six fertilization alternatives (three doses and two alternative sources of application) and a commercial control, were evaluated. The contents of the macronutrients N, P, K, Ca, Mg, S, and chlorine in the leaves were evaluated. The leaf analyses performed from vegetative growth to f lowering showed differences in the foliar contents of nitrogen, calcium, magnesium, and chlorine between the two municipalities and over time, with contents that increased. During this stage, the elements with higher accumulations in the plants included N, K, and Ca. At harvest, the contents of potassium, magnesium, and calcium decreased over time. For nitrogen, sulfur and chlorine, differences were found in the time-municipality interaction

Design of a conservation program assited by genetic markers in a herd of Yacumeño cattle in Santa Cruz – Bolivia

La Facultad de Ciencias Veterinarias de la UAGRM consolido un programa de conservación del ganado Bovino Criollo Yacumeño a partir del año 2004. La conservación de recursos zoogenéticos generalmente se realiza en poblaciones pequeñas, las cuales tienen un tamaño poblacional efectivo muy reducido. En estos casos es importante realizar apareamientos que mantengan la variabilidad genética alta y los niveles de consanguinidad bajos. El objetivo del presente trabajo consistió en determinar las paternidades y los linajes paternos mediante el uso de marcadores moleculares. El ADN se extrajo partir de muestras de sangre de los 149 animales del hato y se procedió a genotipificar todos los individuos utilizando 18 microsatélites y 7 marcadores del cromosoma Y. Los resultados obtenidos permitieron identificar dos grandes grupos de vacas y 3 linajes paternos. Esta información servirá para diseñar un programa reproductivo que evite en lo posible la perdida de la variabilidad genética y mantenga niveles aceptables de consaguinidad en el hato a conservar.The Faculty of Veterinary Science of the UAGRM consolidated a conservation program of the Yacumeño Creole cattle. The conservation of zoogenetic resources is performed generally in small populations, which usually has a reduced effective population size. In those cases it is important to keep high genetic variability and low levels of consanguinity by performing specific mating among the animals. The main objective of this research consisted in determine the paternity and male lines by using molecular markers. The DNA was extracted from blood sample of 149 animals and were genotyped all the individuals using 18 microsatellites and 7 markers of the Y chromosome. The results obtained allowed to identify two groups of cows and 3 male lines. This information will be useful to design a reproductive program that avoids to a certain degree the lost of genetic variability and will keep acceptable levels of consanguinity among the herd.Fil: Pereira, J. A. C.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Carino, M. H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Hoyos, R.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Loza, A.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Mamani, T.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentin

Design of a Conservation Program Assited by Genetic Markers in a Herd of YacumeñO Cattle in Santa Cruz – Bolivia

La Facultad de Ciencias Veterinarias de la UAGRM consolido un programa de conservación del ganado Bovino Criollo Yacumeño a partir del año 2004. La conservación de recursos zoogenéticos generalmente se realiza en poblaciones pequeñas, las cuales tienen un tamaño poblacional efectivo muy reducido. En estos casos es importante realizar apareamientos que mantengan la variabilidad genética alta y los niveles de consanguinidad bajos. El objetivo del presente trabajo consistió en determinar las paternidades y los linajes paternos mediante el uso de marcadores moleculares. El ADN se extrajo partir de muestras de sangre de los 149 animales del hato y se procedió a genotipificar todos los individuos utilizando 18 microsatélites y 7 marcadores del cromosoma Y. Los resultados obtenidos permitieron identificar dos grandes grupos de vacas y 3 linajes paternos. Esta información servirá para diseñar un programa reproductivo que evite en lo posible la perdida de la variabilidad genética y mantenga niveles aceptables de consaguinidad en el hato a conservar.The Faculty of Veterinary Science of the UAGRM consolidated a conservation program of the Yacumeño Creole cattle. The conservation of zoogenetic resources is performed generally in small populations, which usually has a reduced effective population size. In those cases it is important to keep high genetic variability and low levels of consanguinity by performing specific mating among the animals. The main objective of this research consisted in determine the paternity and male lines by using molecular markers. The DNA was extracted from blood sample of 149 animals and were genotyped all the individuals using 18 microsatellites and 7 markers of the Y chromosome. The results obtained allowed to identify two groups of cows and 3 male lines. This information will be useful to design a reproductive program that avoids to a certain degree the lost of genetic variability and will keep acceptable levels of consanguinity among the herd.Instituto de Genética Veterinari

Design of a Conservation Program Assited by Genetic Markers in a Herd of YacumeñO Cattle in Santa Cruz – Bolivia

La Facultad de Ciencias Veterinarias de la UAGRM consolido un programa de conservación del ganado Bovino Criollo Yacumeño a partir del año 2004. La conservación de recursos zoogenéticos generalmente se realiza en poblaciones pequeñas, las cuales tienen un tamaño poblacional efectivo muy reducido. En estos casos es importante realizar apareamientos que mantengan la variabilidad genética alta y los niveles de consanguinidad bajos. El objetivo del presente trabajo consistió en determinar las paternidades y los linajes paternos mediante el uso de marcadores moleculares. El ADN se extrajo partir de muestras de sangre de los 149 animales del hato y se procedió a genotipificar todos los individuos utilizando 18 microsatélites y 7 marcadores del cromosoma Y. Los resultados obtenidos permitieron identificar dos grandes grupos de vacas y 3 linajes paternos. Esta información servirá para diseñar un programa reproductivo que evite en lo posible la perdida de la variabilidad genética y mantenga niveles aceptables de consaguinidad en el hato a conservar.The Faculty of Veterinary Science of the UAGRM consolidated a conservation program of the Yacumeño Creole cattle. The conservation of zoogenetic resources is performed generally in small populations, which usually has a reduced effective population size. In those cases it is important to keep high genetic variability and low levels of consanguinity by performing specific mating among the animals. The main objective of this research consisted in determine the paternity and male lines by using molecular markers. The DNA was extracted from blood sample of 149 animals and were genotyped all the individuals using 18 microsatellites and 7 markers of the Y chromosome. The results obtained allowed to identify two groups of cows and 3 male lines. This information will be useful to design a reproductive program that avoids to a certain degree the lost of genetic variability and will keep acceptable levels of consanguinity among the herd.Instituto de Genética Veterinari

Tetrahydropyrazolo[1,5-a]Pyrimidine-3-Carboxamide and N-Benzyl-6′,7′-Dihydrospiro[Piperidine-4,4′-Thieno[3,2-c]Pyran] analogues with bactericidal efficacy against Mycobacterium tuberculosis targeting MmpL3

Mycobacterium tuberculosis is a major human pathogen and the causative agent for the pulmonary disease, tuberculosis (TB). Current treatment programs to combat TB are under threat due to the emergence of multi-drug and extensively-drug resistant TB. As part of our efforts towards the discovery of new anti-tubercular leads, a number of potent tetrahydropyrazolo[1,5-a]pyrimidine-3-ca​rboxamide(THPP) and N-benzyl-6′,7′-dihydrospiro[piperidine-4,​4′-thieno[3,2-c]pyran](Spiro) analogues were recently identified against Mycobacterium tuberculosis and Mycobacterium bovis BCG through a high-throughput whole-cell screening campaign. Herein, we describe the attractive in vitro and in vivo anti-tubercular profiles of both lead series. The generation of M. tuberculosis spontaneous mutants and subsequent whole genome sequencing of several resistant mutants identified single mutations in the essential mmpL3 gene. This ‘genetic phenotype’ was further confirmed by a ‘chemical phenotype’, whereby M. bovis BCG treated with both the THPP and Spiro series resulted in the accumulation of trehalose monomycolate. In vivo efficacy evaluation of two optimized THPP and Spiro leads showed how the compounds were able to reduce >2 logs bacterial cfu counts in the lungs of infected mice

The Mars Environmental Dynamics Analyzer, MEDA. A Suite of Environmental Sensors for the Mars 2020 Mission

86 pags, 49 figs, 24 tabsNASA's Mars 2020 (M2020) rover mission includes a suite of sensors to monitor current environmental conditions near the surface of Mars and to constrain bulk aerosol properties from changes in atmospheric radiation at the surface. The Mars Environmental Dynamics Analyzer (MEDA) consists of a set of meteorological sensors including wind sensor, a barometer, a relative humidity sensor, a set of 5 thermocouples to measure atmospheric temperature at ∼1.5 m and ∼0.5 m above the surface, a set of thermopiles to characterize the thermal IR brightness temperatures of the surface and the lower atmosphere. MEDA adds a radiation and dust sensor to monitor the optical atmospheric properties that can be used to infer bulk aerosol physical properties such as particle size distribution, non-sphericity, and concentration. The MEDA package and its scientific purpose are described in this document as well as how it responded to the calibration tests and how it helps prepare for the human exploration of Mars. A comparison is also presented to previous environmental monitoring payloads landed on Mars on the Viking, Pathfinder, Phoenix, MSL, and InSight spacecraft.This work has been funded by the Spanish Ministry of Economy and Competitiveness, through the projects No. ESP2014-54256-C4-1-R (also -2-R, -3-R and -4-R) and AYA2015-65041-P; Ministry of Science, Innovation and Universities, projects No. ESP2016-79612-C3-1-R (also -2-R and -3-R), ESP2016-80320-C2-1-R, RTI2018-098728-B-C31 (also -C32 and -C33) and RTI2018-099825-B-C31; Instituto Nacional de Tecnica Aeroespacial; Ministry of Science and Innovation's Centre for the Development of Industrial Technology; Grupos Gobierno Vasco IT1366-19; and European Research Council Consolidator Grant no 818602.Peer reviewe

Gestión del conocimiento: perspectiva multidisciplinaria. Volumen 13

El libro “Gestión del Conocimiento. Perspectiva Multidisciplinaria”, Volumen 13 de la Colección Unión Global, es resultado de investigaciones. Los capítulos del libro, son resultados de investigaciones desarrolladas por sus autores. El libro es una publicación internacional, seriada, continua, arbitrada, de acceso abierto a todas las áreas del conocimiento, orientada a contribuir con procesos de gestión del conocimiento científico, tecnológico y humanístico. Con esta colección, se aspira contribuir con el cultivo, la comprensión, la recopilación y la apropiación social del conocimiento en cuanto a patrimonio intangible de la humanidad, con el propósito de hacer aportes con la transformación de las relaciones socioculturales que sustentan la construcción social de los saberes y su reconocimiento como bien público. El libro “Gestión del Conocimiento. Perspectiva Multidisciplinaria”, Volumen 13, de la Colección Unión Global, es resultado de investigaciones. Los capítulos del libro, son resultados de investigaciones desarrolladas por sus autores. El libro cuenta con el apoyo de los grupos de investigación: Universidad Sur del Lago “Jesús María Semprúm” (UNESUR) - Zulia – Venezuela; Universidad Politécnica Territorial de Falcón Alonso Gamero (UPTFAG) - Falcón – Venezuela; Universidad Politécnica Territorial de Mérida Kléber Ramírez (UPTM) - Mérida - Venezuela; Universidad Guanajuato (UG) - Campus Celaya - Salvatierra - Cuerpo Académico de Biodesarrollo y Bioeconomía en las Organizaciones y Políticas Públicas (CABBOPP) - Guanajuato – México; Centro de Altos Estudios de Venezuela (CEALEVE) - Zulia – Venezuela, Centro Integral de Formación Educativa Especializada del Sur (CIFE - SUR) - Zulia – Venezuela; Centro de Investigaciones Internacionales SAS (CEDINTER) - Antioquia – Colombia y diferentes grupos de investigación del ámbito nacional e internacional que hoy se unen para estrechar vínculos investigativos, para que sus aportes científicos formen parte de los libros que se publiquen en formatos digital e impreso

Effect of fertilization on the contents of macronutrients and chlorine in tobacco leaves cv. flue-cured (<i>Nicotiana tabacum</i> L.) in two municipalities in Huila, Colombia

In Colombia, there is little information on tobacco crops and the effect of fertilization on the nutrient content of leaves. The aim of this study was to determine the contents of mineral nutrients in tobacco (Nicotiana tabacum L. cv. flue-cured) plants subjected to different fertilizer rates and sources in two municipalities in the Huila Province (Colombia). Six foliar analyses, with three measurements from vegetative growth to f lowering and three measurements during harvest, were carried out. Seven treatments, including six fertilization alternatives (three doses and two alternative sources of application) and a commercial control, were evaluated. The contents of the macronutrients N, P, K, Ca, Mg, S, and chlorine in the leaves were evaluated. The leaf analyses performed from vegetative growth to f lowering showed differences in the foliar contents of nitrogen, calcium, magnesium, and chlorine between the two municipalities and over time, with contents that increased. During this stage, the elements with higher accumulations in the plants included N, K, and Ca. At harvest, the contents of potassium, magnesium, and calcium decreased over time. For nitrogen, sulfur and chlorine, differences were found in the time-municipality interaction

Phytosociology of weeds associated with rice crops in the department of Tolima, Colombia

Phytosociological studies allow for the characterization and descriptive analysis of weed communities in crops. This study aimed to characterize the weed communities associated with weed crops in the "Centro, Meseta, and Norte" zones of the Tolima Department. The study was conducted in 96 commercial lots, in which a 1 ha area was marked off for the sampling. The development stage, density and cover of the weeds were evaluated. The importance value index, the alpha diversity indices of Shannon-Wiener, Simpson and uniformity as well as the similarity indices of Jaccard, Sorensen and Steinhaus were calculated. For the entire department, 42 weed species were identified, with Echinochloa colona being the principal one in all of the zones. In the Centro zone, 27 species were identified; in the Meseta zone, 31 species were identified; and, in the Norte zone, 38 species were identified. The alpha indices demonstrated that the Meseta zone was the most diverse. The Jaccard and Sorensen indices showed dissimilarity in the weed community for all of the comparisons of the zones. The Steinhaus coefficient registered the highest similarity intensity between the Centro and Norte zones

PERFIL BIOQUÍMICO RENAL DEL COCODRILO DE TUMBES (Crocodylus acutus) CRIADO EN CAUTIVERIO EN EL NORTE DEL PERÚ

The aim of the study was to determine the biochemistry profile of the kidney in the Tumbes crocodile (Crocodylus acutus) reared in captivity in the north of Peru (Tumbes). Blood samples (6 ml) by puncture of the post-occipital venous sinus were collected to 60 males (24 juveniles, 27 sub-adults, and 9 adults). All animals were manually restrained. Values for uric acid was 74.2 ± 25.0 mg/L, creatinine 1.49 ± 0.39 mg/L, calcium 10.8 ± 2.4 mg/dl, and phosphorus 5.3 ± 2.0 mg/dl. There was significant difference (p&lt;0.05) between juveniles and sub-adults for uric acid and creatinine, and adults versus younger animals for calcium, whereas phosphorus values were not affected by age.El objetivo del estudio fue determinar el perfil bioquímico renal del cocodrilo de Tumbes (Crocodylus acutus) criado en cautiverio en Tumbes, Perú. Los animales fueron contenidos mediante sujeción manual y se colectaron muestras de sangre (6 ml) mediante punción del seno venoso post-occipital a 60 animales machos (24 juveniles, 27 subadultos y 9 adultos). El valor de ácido úrico fue de 74.2 ± 25.0 mg/L, creatinina: 1.49 ± 0.39 mg/L, calcio: 10.8 ± 2.4 mg/dl y fósforo: de 5.3 ± 2.0 mg/dl. Se encontró diferencia estadística (p&lt;0.05) entre juveniles y sub-adultos para el ácido úrico y creatinina, y entre adultos frente a juveniles y sub-adultos para el calcio. No hubo diferencia significativa entre valores de fósforo por grupo etario