Floral patterns in the California Current System off southern California: 1990–1996

In 1990, CalCOFI cruises began routine collection of samples for the enumeration of phytoplankton species. From each quarterly cruise, nearsurface samples from 34 stations are pooled into four regional samples prior to counting. This paper summarizes the first 6.5 years of the program in order to identify major large-scale patterns of species composition and fluctuations. A total of 312 species were recognized during this study. Recurrent group analysis defines two major floral clusters. The first is composed of diatoms characteristic of enriched regions. This cluster is most abundant in the northeastern region and often attains maximum abundances in the spring. Seventy-two percent of the variability of chlorophyll is accounted for by the variability of these species. The second cluster is composed of species common in the offshore central North Pacific. These species have relatively low spatial and temporal variability in the study region. There is no detectable seasonality. In neither cluster can interannual variability be detected above seasonal variability, spatial variability and error. These patterns differ from the geographic patterns of zooplankton species in the region, which are often dominated by fauna from the subarctic North Pacific and transition zone. The apparent absence of a similar subarctic flora is briefly discussed

Floral patterns in the California Current: The coastal-offshore boundary zone

This study quantified the vertical distributions of 294 taxa of phytoplankton from 112 samples in a vertical section across the California Current system off Southern California. The goal was to examine the relationship between coastal and offshore floras throughout the euphotic zone. The two associations were distinct throughout the upper 140 m. The coastal flora was found in water adjacent to and east of the Santa Rosa Ridge. The offshore flora occurred west of the California Current, and also at the station immediately east of the current, presumably the result of upstream entrainment across it. Both coastal and offshore floras were similar to the analogous floras described from the mixed layers in the springs of 1993 and 1995, in spite of different ENSO conditions prevailing during the three years.There were two unexpected results. Although the hydrographic characteristics of offshore water had been strongly modified by mixing with California Current water, the offshore flora remained similar to that of a central Pacific study site, 3000 km to the west. Clearly, the maintenance of this species association is not directly dependent upon temperature or salinity. In contrast, even though the temperature-salinity relationship of the California Current fell within the envelop of T-S relationships of source water to the north, the floristic analyses identified no flora unique to the California Current. Absence of oceanic subarctic species in the Current may be related to upstream depletion of nutrients

Environmental heterogeneity and plankton community structure in the central North Pacific

Spatial and temporal patterns of heterogeneity in nutrients (PO4, NO3), integrated water column chlorophyll, integrated water column primary production, and macrozooplankton biomass in the central North Pacific are described on spatial scales ranging from less than one to several thousand kilometers and on temporal scales from one day to 12 years. Fluctuations in these properties represent an index of the biological response of the ecosystem to physical forcing on various scales. These patterns are an important aspect of ecosystem structure because environmental perturbations may affect the outcome of biological interactions between populations. Heterogeneity in each property was low on all scales. Diel changes were evident only in macrozooplankton biomass, and no seasonal cycles were detected. This is consistent with a low overall level of physical forcing, little advection from outside the system into it, and lack of seasonal changes in nutrient flux to the euphotic zone. The central North Pacific shows relatively low heterogeneity, especially on mesoscales (tens to hundreds of kilometers), when compared to other pelagic ecosystems, suggesting that environmental disturbances do not have a major effect upon macrozooplankton and nekton populations

A statistical analysis of subsampling and an evaluation of the Folsom plankton splitter

Subsampling techniques are important for the determination of precise plankton density estimates. A binomial model of random subsampling, and its Poisson extension, were developed for the purpose of evaluating the performance of compartment-type plankton subsamplers. Two approaches were used to assess the performance of the Folsom plankton splitter on an extensive series of nearshore Lake Michigan crustacean zooplankton samples collected between 1974 and 1979. First, Folsom subsamples were observed to be significantly (p < 0.05) more variable than expected from the random model of subsampling. Second, a random effects ANOVA model was used to compare fractions of the total variance in density estimates that were attributable to subsampling and sampling phases of a specially designed study. Departures from randomness in subsampling were sufficiently small that an analysis of optimal allocation of effort between subsampling and sampling phases, based on the ANOVA model, indicated that only one to three subsamples needed to be examined per sample.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42920/1/10750_2004_Article_BF00016403.pd

Phytoplankton across Tropical and Subtropical Regions of the Atlantic, Indian and Pacific Oceans

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Nitrogen fixation and transfer in open ocean diatom–cyanobacterial symbioses

Many diatoms that inhabit low-nutrient waters of the open ocean live in close association with cyanobacteria. Some of these associations are believed to be mutualistic, where N2-fixing cyanobacterial symbionts provide N for the diatoms. Rates of N2 fixation by symbiotic cyanobacteria and the N transfer to their diatom partners were measured using a high-resolution nanometer scale secondary ion mass spectrometry approach in natural populations. Cell-specific rates of N2 fixation (1.15–71.5 fmol N per cell h−1) were similar amongst the symbioses and rapid transfer (within 30 min) of fixed N was also measured. Similar growth rates for the diatoms and their symbionts were determined and the symbiotic growth rates were higher than those estimated for free-living cells. The N2 fixation rates estimated for Richelia and Calothrix symbionts were 171–420 times higher when the cells were symbiotic compared with the rates estimated for the cells living freely. When combined, the latter two results suggest that the diatom partners influence the growth and metabolism of their cyanobacterial symbionts. We estimated that Richelia fix 81–744% more N than needed for their own growth and up to 97.3% of the fixed N is transferred to the diatom partners. This study provides new information on the mechanisms controlling N input into the open ocean by symbiotic microorganisms, which are widespread and important for oceanic primary production. Further, this is the first demonstration of N transfer from an N2 fixer to a unicellular partner. These symbioses are important models for molecular regulation and nutrient exchange in symbiotic systems

Multivariate statistical analysis of net diatom species distributions in the Southwestern Atlantic and Indian Ocean

Vertical net haul diatom assemblages from near South Georgia, and from between Africa and Antarctica, were examined and compared. Variation among South Georgia stations was examined by principal component, cluster and canonical discriminant analyses. Diatom distributions provide evidence for at least two distinct water masses. The region north of the island is characterized by neritic, temperate diatoms and by an assemblage with low species diversity. The region south of the island is characterized by oceanic, antarctic species and relatively high species diversity. The regions are most distinct to the west of the island, intergrading east of the island. Within the north-south division, five station groupings were detected on the basis of distribution of dominant net diatoms. By comparing classical species ecological categorizations to results of principal component analysis, a “neritic-oceanic” factor was identified from net diatom distributions. This factor was common to both areas in spite of the fact that Biscoe and Agulhas collections were from different seasons.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46982/1/300_2004_Article_BF00446041.pd

Predicting the Electron Requirement for Carbon Fixation in Seas and Oceans

Marine phytoplankton account for about 50% of all global net primary productivity (NPP). Active fluorometry, mainly Fast Repetition Rate fluorometry (FRRf), has been advocated as means of providing high resolution estimates of NPP. However, not measuring CO2-fixation directly, FRRf instead provides photosynthetic quantum efficiency estimates from which electron transfer rates (ETR) and ultimately CO2-fixation rates can be derived. Consequently, conversions of ETRs to CO2-fixation requires knowledge of the electron requirement for carbon fixation (Φe,C, ETR/CO2 uptake rate) and its dependence on environmental gradients. Such knowledge is critical for large scale implementation of active fluorescence to better characterise CO2-uptake. Here we examine the variability of experimentally determined Φe,C values in relation to key environmental variables with the aim of developing new working algorithms for the calculation of Φe,C from environmental variables. Coincident FRRf and 14C-uptake and environmental data from 14 studies covering 12 marine regions were analysed via a meta-analytical, non-parametric, multivariate approach. Combining all studies, Φe,C varied between 1.15 and 54.2 mol e- (mol C)-1 with a mean of 10.9±6.91 mol e- mol C)-1. Although variability of Φe,C was related to environmental gradients at global scales, region-specific analyses provided far improved predictive capability. However, use of regional Φe,C algorithms requires objective means of defining regions of interest, which remains challenging. Considering individual studies and specific small-scale regions, temperature, nutrient and light availability were correlated with Φe,C albeit to varying degrees and depending on the study/region and the composition of the extant phytoplankton community. At the level of large biogeographic regions and distinct water masses, Φe,C was related to nutrient availability, chlorophyll, as well as temperature and/or salinity in most regions, while light availability was also important in Baltic Sea and shelf waters. The novel Φe,C algorithms provide a major step forward for widespread fluorometry-based NPP estimates and highlight the need for further studying the natural variability of Φe,C to verify and develop algorithms with improved accuracy. © 2013 Lawrenz et al

Benthic algal response to N and P enrichment along a pH gradient

Nutrient enrichment and its effect on benthic algal growth, community composition, and average cell size was assessed across two sites of differing pH within a single habitat. Nutrients were added using in situ substrata, which released either N, P, or no additional nutrients (controls) at each site for 21 days. Upon collection, chlorophyll and biovolume standing stocks of the attached algal microflora were measured. Chlorophyll concentration was different among all treatments, accumulating greatest on P, followed by N, and the least on C substrata (P < 0.001) and was highest at site-2 (P < 0.001), while total algal biovolume was highest on P compared to both N and C substrata (P < 0.05) and did not vary between sites. Increased growth on P substrata was due to the enhanced biovolume of filamentous green algae, although the affected taxa varied between sites. Biovolume to cell density ratios (as a measure of average cell size) were highest on P substrata over both N-enriched and control substrata (P < 0.05) and this pattern was similar between sites. Progression towards a community composed of larger cells following P enrichment observed along this pH gradient, seems to be related to the dominance of larger celled filamentous green algae. Thus, nutrients exhibited greater control on benthic algal growth than did changes in hydrogen ion concentration.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42877/1/10750_2004_Article_BF00007599.pd