The assessment of water resources in ungauged catchments in Rwanda

Study region: Rwanda is a landlocked country in Africa with precipitation ranging from 800 mm yr−1 in the east to 1500 mm yr−1 in high-altitude regions in the north and west. Study focus: Streamflow estimation is an important task that is required in water resource assessments due to its importance in planning, decision-making and economic development. In this study, streamflow characteristics of ungauged catchments in Rwanda were calculated using a regionalization approach based on climate similarity and stepwise multiple-regression analysis. One climatic homogeneous region was identified and datasets of nine gauged stations and general available catchment characteristics were used to develop non-transformed and log-transformed regression models. New hydrological insights for the region: Results of this study show that climate, physiography and land cover strongly influence the hydrology of catchments in Rwanda. Using leave-one-out crossvalidation, the log-transformed models were found to predict the flow parameters more suitably. These models can be used for estimating the flow parameters in ungauged catchments in Rwanda and the methodology can be applied in any other region, as long as sufficient and good quality streamflow data is available

Comparative Proteomic Analysis of Serum from Patients with Systemic Sclerosis and Sclerodermatous GVHD. Evidence of Defective Function of Factor H

BACKGROUND: Systemic sclerosis (SSc) is an autoimmune disease characterized by immunological and vascular abnormalities. Until now, the cause of SSc remains unclear. Sclerodermatous graft-versus-host disease (ScGVHD) is one of the most severe complications following bone marrow transplantation (BMT) for haematological disorders. Since the first cases, the similarity of ScGVHD to SSc has been reported. However, both diseases could have different etiopathogeneses. The objective of this study was to identify new serum biomarkers involved in SSc and ScGVHD. METHODOLOGY: Serum was obtained from patients with SSc and ScGVHD, patients without ScGVHD who received BMT for haematological disorders and healthy controls. Bi-dimensional electrophoresis (2D) was carried out to generate maps of serum proteins from patients and controls. The 2D maps underwent image analysis and differently expressed proteins were identified. Immuno-blot analysis and ELISA assay were used to validate the proteomic data. Hemolytic assay with sheep erythrocytes was performed to evaluate the capacity of Factor H (FH) to control complement activation on the cellular surface. FH binding to endothelial cells (ECs) was also analysed in order to assess possible dysfunctions of this protein. PRINCIPAL FINDINGS: Fourteen differentially expressed proteins were identified. We detected pneumococcal antibody cross-reacting with double stranded DNA in serum of all bone marrow transplanted patients with ScGVHD. We documented higher levels of FH in serum of SSc and ScGVHD patients compared healthy controls and increased sheep erythrocytes lysis after incubation with serum of diffuse SSc patients. In addition, we observed that FH binding to ECs was reduced when we used serum from these patients. CONCLUSIONS: The comparative proteomic analysis of serum from SSc and ScGVHD patients highlighted proteins involved in either promoting or maintaining an inflammatory state. We also found a defective function of Factor H, possibly associated with ECs damage

Non-IDH1-R132H IDH1/2 mutations are associated with increased DNA methylation and improved survival in astrocytomas, compared to IDH1-R132H mutations

Somatic mutations in the isocitrate dehydrogenase genes IDH1 and IDH2 occur at high frequency in several tumour types. Even though these mutations are confined to distinct hotspots, we show that gliomas are the only tumour type with an exceptionally high percentage of IDH1(R132H) mutations. Patients harbouring IDH1(R132H) mutated tumours have lower levels of genome-wide DNA-methylation, and an associated increased gene expression, compared to tumours with other IDH1/2 mutations ("non-R132H IDH1/2 mutations"). This reduced methylation is seen in multiple tumour types and thus appears independent of the site of origin. For 1p/19q non-codeleted glioma (astrocytoma) patients, we show that this difference is clinically relevant: in samples of the randomised phase III CATNON trial, patients harbouring tumours with IDH mutations other than IDH1(R132H) have a better outcome (hazard ratio 0.41, 95% CI [0.24, 0.71], p = 0.0013). Such non-R132H IDH1/2-mutated tumours also had a significantly lower proportion of tumours assigned to prognostically poor DNA-methylation classes (p < 0.001). IDH mutation-type was independent in a multivariable model containing known clinical and molecular prognostic factors. To confirm these observations, we validated the prognostic effect of IDH mutation type on a large independent dataset. The observation that non-R132H IDH1/2-mutated astrocytomas have a more favourable prognosis than their IDH1(R132H) mutated counterpart indicates that not all IDH-mutations are identical. This difference is clinically relevant and should be taken into account for patient prognostication.MTG6Molecular tumour pathology - and tumour genetic

The assessment of water resources in ungauged catchments in Rwanda

Study region: Rwanda is a landlocked country in Africa with precipitation ranging from 800 mm yr−1 in the east to 1500 mm yr−1 in high-altitude regions in the north and west. Study focus: Streamflow estimation is an important task that is required in water resource assessments due to its importance in planning, decision-making and economic development. In this study, streamflow characteristics of ungauged catchments in Rwanda were calculated using a regionalization approach based on climate similarity and stepwise multiple-regression analysis. One climatic homogeneous region was identified and datasets of nine gauged stations and general available catchment characteristics were used to develop non-transformed and log-transformed regression models. New hydrological insights for the region: Results of this study show that climate, physiography and land cover strongly influence the hydrology of catchments in Rwanda. Using leave-one-out crossvalidation, the log-transformed models were found to predict the flow parameters more suitably. These models can be used for estimating the flow parameters in ungauged catchments in Rwanda and the methodology can be applied in any other region, as long as sufficient and good quality streamflow data is available

Conscious awareness is necessary to assess trust and mimic facial expressions, while pupils impact trust unconsciously

People make rapid inferences about others' thoughts and intentions. For example, they observe facial movements and pupil size of others and unwittingly make use of this information when deciding whether to trust someone or not. However, whether spontaneous mimicry depends on visual awareness of the stimulus and whether these processes underlie trust decisions is still unknown. To investigate whether visual awareness modulates the relationship between emotional expressions, mimicry and trust, participants played a series of trust games and saw either their partners' faces with a neutral, happy or fearful expression, or their partners' eyes in which the pupil size was large, medium or small. Subjects' trust investments, facial movements and pupil responses were measured. In half of the trials, the stimuli were rendered invisible by continuous flash suppression. Results showed that facial expressions were mimicked and influenced trust decisions during the conscious condition, but not during the unconscious (suppressed) condition. The opposite was found for pupil size, which influenced trust decisions during states of unawareness. These results suggest that the neurobiological pathway linking the observation of facial expressions to mimicry and trust is predominantly conscious, whereas partner pupil size influences trust primarily when presented unconsciously. This article is part of the theme issue 'Cracking the laugh code: laughter through the lens of biology, psychology and neuroscience'

Conscious awareness is necessary to assess trust and mimic facial expressions, while pupils impact trust unconsciously

People make rapid inferences about others' thoughts and intentions. For example, they observe facial movements and pupil size of others and unwittingly make use of this information when deciding whether to trust someone or not. However, whether spontaneous mimicry depends on visual awareness of the stimulus and whether these processes underlie trust decisions is still unknown. To investigate whether visual awareness modulates the relationship between emotional expressions, mimicry and trust, participants played a series of trust games and saw either their partners' faces with a neutral, happy or fearful expression, or their partners' eyes in which the pupil size was large, medium or small. Subjects' trust investments, facial movements and pupil responses were measured. In half of the trials, the stimuli were rendered invisible by continuous flash suppression. Results showed that facial expressions were mimicked and influenced trust decisions during the conscious condition, but not during the unconscious (suppressed) condition. The opposite was found for pupil size, which influenced trust decisions during states of unawareness. These results suggest that the neurobiological pathway linking the observation of facial expressions to mimicry and trust is predominantly conscious, whereas partner pupil size influences trust primarily when presented unconsciously. This article is part of the theme issue 'Cracking the laugh code: laughter through the lens of biology, psychology and neuroscience'

Rainfall characteristics and regionalization in peninsular malaysia based on a high resolution gridded data set

Daily gridded rainfall data over Peninsular Malaysia are delineated using an objective clustering algorithm, with the objective of classifying rainfall grids into groups of homogeneous regions based on the similarity of the rainfall annual cycles. It has been demonstrated that Peninsular Malaysia can be statistically delineated into eight distinct rainfall regions. This delineation is closely associated with the topographic and geographic characteristics. The variation of rainfall over the Peninsula is generally characterized by bimodal variations with two peaks, i.e., a primary peak occurring during the autumn transitional period and a secondary peak during the spring transitional period. The east coast zones, however, showed a single peak during the northeast monsoon (NEM). The influence of NEM is stronger compared to the southwest monsoon (SWM). Significantly increasing rainfall trends at 95% confidence level are not observed in all regions during the NEM, with exception of northwest zone (R1) and coastal band of west coast interior region (R3). During SWM, most areas have become drier over the last three decades. The study identifies higher variation of mean monthly rainfall over the east coast regions, but spatially, the rainfall is uniformly distributed. For the southwestern coast and west coast regions, a larger range of coefficients of variation is mostly obtained during the NEM, and to a smaller extent during the SWM. The inland region received least rainfall in February, but showed the largest spatial variation. The relationship between rainfall and the El Niño Southern Oscillation (ENSO) was examined based on the Multivariate ENSO Index (MEI). Although the concurrent relationships between rainfall in the different regions and ENSO are generally weak with negative correlations, the rainfall shows stronger positive correlation with preceding ENSO signals with a time lag of four to eight months

Rainfall characteristics and regionalization in peninsular malaysia based on a high resolution gridded data set

Daily gridded rainfall data over Peninsular Malaysia are delineated using an objective clustering algorithm, with the objective of classifying rainfall grids into groups of homogeneous regions based on the similarity of the rainfall annual cycles. It has been demonstrated that Peninsular Malaysia can be statistically delineated into eight distinct rainfall regions. This delineation is closely associated with the topographic and geographic characteristics. The variation of rainfall over the Peninsula is generally characterized by bimodal variations with two peaks, i.e., a primary peak occurring during the autumn transitional period and a secondary peak during the spring transitional period. The east coast zones, however, showed a single peak during the northeast monsoon (NEM). The influence of NEM is stronger compared to the southwest monsoon (SWM). Significantly increasing rainfall trends at 95% confidence level are not observed in all regions during the NEM, with exception of northwest zone (R1) and coastal band of west coast interior region (R3). During SWM, most areas have become drier over the last three decades. The study identifies higher variation of mean monthly rainfall over the east coast regions, but spatially, the rainfall is uniformly distributed. For the southwestern coast and west coast regions, a larger range of coefficients of variation is mostly obtained during the NEM, and to a smaller extent during the SWM. The inland region received least rainfall in February, but showed the largest spatial variation. The relationship between rainfall and the El Niño Southern Oscillation (ENSO) was examined based on the Multivariate ENSO Index (MEI). Although the concurrent relationships between rainfall in the different regions and ENSO are generally weak with negative correlations, the rainfall shows stronger positive correlation with preceding ENSO signals with a time lag of four to eight months

Effects of renal failure on complement C3d levels

Elevated plasma concentrations of complement split product C3d have been reported to represent activation of the complement system. In the present study the effect of renal function on C3d concentrations was investigated in patients with various degrees of renal impairment, in patients with chronic renal failure and in CAPD patients. It appeared that elevated plasma C3d concentrations were present in patients with plasma creatinine concentrations in excess of 200 mumol/l regardless of the type of kidney disease. It is very likely that this can be attributed to renal handling (i.e. glomerular filtration, tubular reabsorption and renal catabolism) of C3d in a similar way as has been demonstrated for other low molecular weight proteins. The peritoneal permeability to C3d was slightly less than could be expected on the basis of its molecular weight without evidence of local production of C3d. Renal function should be taken into account in the interpretation of elevated plasma concentrations of C3