Greenhouse Production of Jatropha, a Potential Biofuel Crop

ADDITIONAL INDEX WORDS. biofuel, biodiesel, bioenergy, Jatropha curcas SUMMARY. The objective of this study was to evaluate greenhouse techniques for the production of jatropha ( Jatropha curcas). Jatropha seedlings were transplanted into 1-gal containers filled with bark mix, coir, or peat-based substrate and fertilized with 0, 4.1, 5.9, or 8.3 oz/ft 3 of a 15N-4.05P-9.96K controlled-release fertilizer (CRF). Plants were watered every 2, 3, or 4 days for 80 days in the greenhouse. Jatropha plants grown in peat-based substrate had greater stem diameter and shoot dry weight (SDW) than plants grown in bark mix. For each growing substrate, plants fertilized with 8.3 and 5.9 oz/ft 3 of CRF had greater SDW than plants fertilized with 4.1 and 0 oz/ft 3 of CRF. Similarly, for all three substrates, plants irrigated every 2 or 3 days had greater SDW than plants irrigated every 4 days. Although jatropha has been classified as a low-nutrient and water requiring plant, the results of this study suggest that increased inputs of fertilizer and water produce larger plants. Further research needs to be conducted on the benefit of larger plants from the greenhouse on subsequent oil production in the field

Phloroglucinol is Effective for in vitro Growth and Multiplication of Musa accuminata Cv. Grand Naine Shoots and Roots

Despite being a major staple food in the world, banana production in the United States is still limited, with about 500 acres under cultivation. Micropropagation has been an effective method for the large-scale production of bananas to meet both domestic and international markets. However, the efficiency of micropropagation protocols depends on several factors, particularly on the types, combinations, and levels of plant growth regulators used in the culture media. Phloroglucinol is a growth regulator that acts synergistically with auxins and cytokinins. The use of phloroglucinol for the production and development of in vitro plantlets of Musa spp. cv. Grande Naine were investigated. Multiplication and elongation of shoots and roots in vitro was enhanced by the addition of 200 μM phloroglucinol to MS medium, as compared to the control with 13.2 μM BA. Higher concentrations (400 to 1000 μM phloroglucinol) resulted in reduced growth and development of shoots and roots in vitro

Determining the preliminary performance of selected Annonaceae cultivars in subtropical conditions

Plants phenology is extremely important tool for crop management, crop manipulation, and marketing. The objective of this research was to investigate the phenology and fruit quality of the several Annonaceae species under subtropical conditions of Homestead, Florida, USA. The study was carried out in an experimental sugar apple (Annona squamosa L.), orchard with cultivars: 'LeahReese', 'Lessard Thai' and 'Red' and the atemoya ‘Gefner’ (A. cherimola x A. squamosa L). Ten uniformly vigorous and healthy plants of each cultivar were selected. The fruit harvest period was similar for all the cultivars, except for sugar-apple 'LeahReese', which had the shortest harvest period of only 41 days. Multivariate analysis showed that 'Lessard Thai' and 'Red' sugar apple cultivars had smaller sizes and lower chemical quality. Cultivar "LeahResse" had the highest fruits, in addition, a strong correlation with the atemoya hybrid "Gefner". The sugar apples ‘Lessard Thai’ and 'Red' had similar phenology and had a 166-day pruning to harvest cycle. In contrast, 'LeahReese' sugar apple and 'Gefner' atemoya had significantly longer cycles of 182 and 196 days, respectively. The sugar apple 'LeahReese' had the best physical characteristics of the cultivars investigated, while the atemoya 'Gefner' had the best physiochemical characteristics and the longest shelf life

Development and validation of a standard area diagram set as assessment aid for estimating the severity of bacterial spot on tri-lobed leaves of yellow passion fruit

Bacterial spot (Xanthomonas axonopodis pv. passiflorae) significantly reduces yellow passion fruit (Passiflora edulis Sims) yield and longevity. A standard area diagram set (SADs) for severity assessment of bacterial spot on tri-lobed leaves of yellow passion was developed and validated in this study. The SADs consisted of eight severity levels (2; 4; 9; 18; 35; 58; 80; and 94%). For its validation, 20 raters, who initially estimated the disease severity without the aid of the SADs, were divided into four groups (G1 and G3, inexperienced; G2 and G4, experienced). Subsequently, G1 and G2 performed the second evaluation without the SADs, and G3 and G4 completed the second evaluation with the proposed SADs. The accuracy and precision of the assessments were determined by simple linear regression and by the Lin’s concordance correlation coefficient (LCCC). The proposed SADs allowed accurate and precise quantification of bacterial spot severity, increasing the agreement between estimated and actual values. Inexperienced raters benefited the most from the use of the SADs. The increase in accuracy and precision in the non-aided groups, when present, was less pronounced than those increments observed in the SADs-aided groups. The LCCC confirmed the increases in accuracy and precision detected by the linear regression analysis

Yellow passion fruit reaction to Xanthomonas axonopodis pv. passiflorae and to Cowpea aphid-borne mosaic virus

Yellow passion fruit (Passiflora edulis Sims) yield and longevity have been drastically reduced by bacterial spot (Xanthomonas axonopodis pv. passiflorae - Xap) and passion fruit woodiness disease (PWD) (Cowpea aphid-borne mosaic virus - CABMV). This study was aimed at evaluating the reaction of 11 genotypes of yellow passion fruit, based on the reaction of their progenies, to both mechanically inoculated Xap and CABMV, under greenhouse conditions. There was a progressive increase in bacterial spot and PWD severity with time. BRS Gigante Amarelo, MAR20#12, and MAR20#34 were selected as the progenies with lowest bacterial spot severity and disease progress over time. MAR20#2005, EC-L-7, UnB2015-1, and EC-3-0 presented the lowest PWD severity and disease progress over time. These progenies, along with individual plants from other progenies in which disease severity was significantly low until the last evaluation, will be cloned and tested again for Xap and CABMV, including other isolates

Effect of plant-growth regulators, adjuvants and artificial pollination on the fixation and quality of Annona squamosa L cultivars’ fruit

The aim of the study was to assess the effect of plant regulators (gibberellic acid and naphthalene acetic acid) and adjuvant LI 700® with and without hand pollination on the fruiting and quality of Annona squamosa L. cultivars. The experiment was conducted in the experimental orchard of the Florida Tropical Research and Education Center (TREC/UF) in Homestead, Florida, USA. The experimental design in random blocks comprised 14 treatments, 10 repetitions and 3 flowers per treatment and per tree for ‘Red’ and ‘Lessard Thai’ cultivars. To assess quality, 9 fruit from each treatment were used, with 3 repetitions  and 3 fruit per repetition. High instability was observed in fruit fixation rates between cultivars. The application of growth regulators did not increase fruit fixation in the tested cultivars. The application of naphthalene acetic acid had a deleterious effect on the flowers of the sugar apple cultivars. The most uneven (asymmetrical) fruit was obtained in the treatments with the application of growth regulators. The application of the adjuvant showed toxicity, causing damage to the skin of the fruit. The fruit with the greatest symmetry had the lowest levels of soluble solids and the highest toxicity. The application of growth regulators associated with hand pollination was not efficient, presenting a low percentage of fruit fixation for Annonaceae cultivars. None of the studied treatments produced parthenocarpic fruit (without seeds). The utilization of an adjuvant in the applied concentration caused damage to the fruit peels (darkening) due to toxicity in both cultivars

Germination, carbohydrate composition and vigor of cryopreserved Caesalpinia echinata seeds

The present study investigated the germination and vigor of Caesalpinia echinata (Brazilwood) seeds stored at negative temperatures. Recently harvested seeds were cryopreserved at -18º or -196ºC and periodically evaluated for germination, seed vigor and carbohydrate composition. The temperatures did not influence the germination percentages or vigor. The germination percentage decreased from 88% in recently harvested seeds to 60% after 730 days of storage. The different temperature and storage times tested did not affect the vigor seed germination as indicated by the measures of plant growth and survival. The different temperatures used did not cause changes in the carbohydrate composition. The tegument cell walls were rich in lignin, arabinose and xylose. The cytoplasm of the cotyledons and embryos had high levels of glucose, fructose, and sucrose. The cryopreservation technique here presented was effective in the conservation of Brazilwood seeds for the medium term

IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production

IL-15 is a key regulator of NK cell maintenance and proliferation and synergizes with other myeloid cell-derived cytokines to enhance NK cell effector function. At low concentrations, trans-presentation of IL-15 by dendritic cells can activate NK cells, whereas at higher concentrations it can act directly on NK cells, independently of accessory cells. In this study, we investigate the potential for IL-15 to boost responses to influenza virus by promoting accessory cell function. We find that coculture of human PBMCs with inactivated whole influenza virus (A/Victoria/361/2011) in the presence of very low concentrations of IL-15 results in increased production of myeloid cell-derived cytokines, including IL-12, IFN-α2, GM-CSF, and IL-1β, and an increased frequency of polyfunctional NK cells (defined by the expression of two or more of CD107a, IFN-γ, and CD25). Neutralization experiments demonstrate that IL-15-mediated enhancement of NK cell responses is primarily dependent on IL-12 and partially dependent on IFN-αβR1 signaling. Critically, IL-15 boosted the production of IL-12 in influenza-stimulated blood myeloid dendritic cells. IL-15 costimulation also restored the ability of less-differentiated NK cells from human CMV-seropositive individuals to respond to influenza virus. These data suggest that very low concentrations of IL-15 play an important role in boosting accessory cell function to support NK cell effector functions