CONFINEMENT OF MICRONUCLEUS ON DAWKINSIA TAMBRAPARNIEI BY THE PARADOXICAL EFFECT OF THE ARSENIC DERIVATIVES

Objective: In the current study, in vivo genotoxic effects of arsenic derivatives such as arsenic trioxide (As2O3) and sodium arsenite (NaAsO2) on peripheral blood erythrocytes of Dawkinsia tambraparniei were investigated using the micronucleus (MN) test.Methods: MN staining was done using acridine orange pre-coated slides. Fluorescent microscope was used for scoring.Results: In NaAsO2 exposed, the erythrocytes highest value was recorded at 42 days which is twofold higher than exposure at 7 days when compared to As2O3. Highest percentage was recorded about 13.9 in NaAsO2, and in case of As2O3, it was recorded as 0.2% less. It was clearly confirmed that either form of arsenic is toxic to organism.Conclusion: Anthropogenic activities have also brought in substantial amounts of them into the environment by mobilization from their natural insoluble deposits or environmental sins. Hence, arsenic pollution should have measured, and arsenic removal process should have carried out

Evaluation of appendicitis risk prediction models in adults with suspected appendicitis

Background Appendicitis is the most common general surgical emergency worldwide, but its diagnosis remains challenging. The aim of this study was to determine whether existing risk prediction models can reliably identify patients presenting to hospital in the UK with acute right iliac fossa (RIF) pain who are at low risk of appendicitis. Methods A systematic search was completed to identify all existing appendicitis risk prediction models. Models were validated using UK data from an international prospective cohort study that captured consecutive patients aged 16–45 years presenting to hospital with acute RIF in March to June 2017. The main outcome was best achievable model specificity (proportion of patients who did not have appendicitis correctly classified as low risk) whilst maintaining a failure rate below 5 per cent (proportion of patients identified as low risk who actually had appendicitis). Results Some 5345 patients across 154 UK hospitals were identified, of which two‐thirds (3613 of 5345, 67·6 per cent) were women. Women were more than twice as likely to undergo surgery with removal of a histologically normal appendix (272 of 964, 28·2 per cent) than men (120 of 993, 12·1 per cent) (relative risk 2·33, 95 per cent c.i. 1·92 to 2·84; P < 0·001). Of 15 validated risk prediction models, the Adult Appendicitis Score performed best (cut‐off score 8 or less, specificity 63·1 per cent, failure rate 3·7 per cent). The Appendicitis Inflammatory Response Score performed best for men (cut‐off score 2 or less, specificity 24·7 per cent, failure rate 2·4 per cent). Conclusion Women in the UK had a disproportionate risk of admission without surgical intervention and had high rates of normal appendicectomy. Risk prediction models to support shared decision‐making by identifying adults in the UK at low risk of appendicitis were identified

EFFICACY OF MICRODOSES POTENTIZED HOMEOPATHIC DRUG, ARSENICUM ALBUM INDUCED BY ARSENIC TRIOXIDE IN ZEBRAFISH (DANIO RERIO)-A FTIR STUDY IN GILLS

Objective: Arsenic, one of the major natural as well as anthropogenic pollutant produces biochemical changes in the organs of animals due to its potential toxicity. Arsenic is toxic to fish and may be taken through gills and skin. In the current work, arsenic trioxide-treated fishes were prescribed with potentized homoeopathic drug, ars alb 30C, and ars alb 200C. The biochemical contents in exposed gill tissues of zebrafish (Danio rerio) were analysed using Fourier transform infrared spectroscopy (FTIR).Methods: The acclimatised fish were treated with arsenic trioxide and LC50 was calculated. Along with arsenic trioxide, the homoeopathic drug ars alb 30C and ars alb 200C were given three drops per litre. After a period of 7 and 14 d, the gills of exposed fish were dissected. The samples were lyophilized and analysed in FTIR.Results: In arsenic exposed fish, the amine N-H stretch was different when compared to the control and the treated different potencies of ars alb 30C and 200C respectively at 7days. Whereas at 14 d the arsenic exposed fish the amine N-H stretch was drastically reduced. Notably, in treating different potencies of ars alb 30C and 200C, the amine N-H stretch was increased.Conclusion: It was found that the higher the potency that is ars alb 200C was more effective against Arsenic toxicity. It shows higher dilution may be efficacious

Mastering Kali Linux for advanced penetration testing: secure your network with Kali Linux 2019.1 : the ultimate white hat hackers' toolkit

Mastering Kali Linux for Advanced Penetration Testing, Third edition will provide you with a number of proven techniques to defeat the latest network defenses using Kali Linux. From selecting the most effective tools, to rapidly compromising any given network/system/application infrastructure security, escalating privileges and highlighting ..

Correction: Assessment of lung function in successfully treated tuberculosis reveals high burden of ventilatory defects and COPD.

[This corrects the article DOI: 10.1371/journal.pone.0217289.]