Preservation of anti-SARS-CoV-2 neutralising antibodies in convalescent plasma after pathogen reduction with methylene blue and visible light

Background - COVID-19 convalescent plasma (CCP) is an experimental treatment against SARS-CoV-2. Although there has so far been no evidence of transmission through transfusion, pathogen reduction technologies (PRT) have been applied to CCP to mitigate risk of infectious disease. This study aims to assess the impact of methylene blue (MB) plus visible light PRT on the virus-neutralising activity of the specific antibodies against SARS-CoV-2. Material and methods - Thirty-five plasma doses collected by plasmapheresis from COVID-19 convalescent donors were subjected to MB plus visible light PRT. Anti-SARS-CoV-2 RBD S1 epitope IgGs antibodies were quantified by ELISA. Titres of SARS-CoV-2 neutralising antibodies (NtAbs) were measured before and after the PRT process. A Spearman's correlation was run to determine the relationship between antibody neutralisation ability and SARS-CoV-2 IgG ELISA ratio. Pre- and post-inactivation neutralising antibody titres were evaluated using a Wilcoxon test. Results - The plasma pathogen reduction procedure did not diminish NtAbS titres and so did not cause a change in the viral neutralisation capacity of CCP. There was a strong correlation between pre-and post-PRT NtAbs and anti-SARS-CoV-2 IgGs titres. Discussion - Our results showed PRT with MB did not impair the CCP passive immunity preserving its potential therapeutic potency. Therefore, PRT of CCP should be recommended to mitigate the risk for transmission of transfusionassociated infectious disease. There is a good correlation between SARS-CoV-2 IgG titres determined by ELISA and the neutralising capacity. This allows blood centres to select CCP donors based on IgG ELISA titres avoiding the much more labour-intensive laboratory processes for determining neutralising antibodies.Peer reviewe

Cov-caldas: A new COVID-19 chest X-Ray dataset from state of Caldas-Colombia

The emergence of COVID-19 as a global pandemic forced researchers worldwide in various disciplines to investigate and propose efficient strategies and/or technologies to prevent COVID-19 from further spreading. One of the main challenges to be overcome is the fast and efficient detection of COVID-19 using deep learning approaches and medical images such as Chest Computed Tomography (CT) and Chest X-ray images. In order to contribute to this challenge, a new dataset was collected in collaboration with “S.E.S Hospital Universitario de Caldas” (https://hospitaldecaldas.com/) from Colombia and organized following the Medical Imaging Data Structure (MIDS) format. The dataset contains 7,307 chest X-ray images divided into 3,077 and 4,230 COVID-19 positive and negative images. Images were subjected to a selection and anonymization process to allow the scientific community to use them freely. Finally, different convolutional neural networks were used to perform technical validation. This dataset contributes to the scientific community by tackling significant limitations regarding data quality and availability for the detection of COVID-19. © 2022, The Author(s)

Unidad de soporte de los institutos universitarios del campus de Burjassot-Paterna de la Universitat de València. Situación actual y plan de mejora

[ES] En dicho trabajo se ha analizado la situación actual y se ha presentado un Plan de Mejora de la Unidad de Soporte de Institutos Burjassot‐Paterna de la Universitat de Valènciaobjeto del análisis. La Unidad de Soporte a los Institutos Universitarios de Investigación del Campus de Burjassot – Paterna tiene encomendada la gestión administrativa y económica de los proyectos, contratos y convenios de investigación que promueve el personal investigador adscrito a los mismos, soporte administrativo a los Institutos, así como la gestión de los edificios y las instalaciones auxiliares puestas a disposición de los Institutos de Investigación. Ejerce sus funciones en base a la descentralización administrativa promovida por la Universitat de València, bajo la dependencia orgánica y funcional de la Gerencia.Leiva Vayá, MJ. (2015). UNIDAD DE SOPORTE DE LOS INSTITUTOS UNIVERSITARIOS DEL CAMPUS DE BURJASSOTPATERNA DE LA UNIVERSITAT DE VALÈNCIA. SITUACIÓN ACTUAL Y PLAN DE MEJORA. http://hdl.handle.net/10251/58165.Archivo delegad

Propuesta de elaboración de un manual de procedimientos para la elaboración de expedientes económicos del Departamento de Personalitat, Avaluació i Tractaments Psicològics de la Universitat de València Estudi General

[ES] Proponer la implantación de un Manual de Procedimientos de Expedientes Económicos que sirva de guía para la gestión de los trámites administrativos que se realizan en el Departamento de Personalidad, Evaluación y Tratamientos Psicológicos de la UVEGLeiva Vayá, MJ. (2017). Propuesta de elaboración de un Manual de procedimientos para la elaboración de expedientes económicos del Departamento de Personalitat, Avaluació i Tractaments Psicològics de la Universitat de València Estudi General. http://hdl.handle.net/10251/89314.TFG

Impact of global warming on mycotoxins

The large impacts of global warming projected on crops worldwide will subsequently influence not only food security, by reducing yields and thus food availability, but food and feed safety, mycotoxins being considered one of the most important food safety hazards affected by climate change. Future changes in temperature, precipitation, and atmospheric CO2 concentration are expected to carry along an increased risk of mycotoxin contamination of cereal crops in the field and might have an impact on the geographical distribution of certain cereals, mycotoxigenic fungi and their mycotoxins

Anti-SARS-CoV-2 IgA and IgG in Human Milk From Vaccinated Mothers After Holder Pasteurization

Breastfeeding rendered crucial for preterm infants during COVID-19 pandemic. However, the role of donated human milk (DHM) in the protection against SARS-CoV-2 infection when own mother’s milk is not available or insufficient remains unclear. Pasteurization of DHM contributes to the loss of some biological and nutritional properties of human milk. The presence of specific breast milk SARS-CoV-2 antibodies has been demonstrated in women after COVID-19 disease and after vaccination. We aimed to evaluate the impact of Holder pasteurization on vaccinated donor women’s milk and to identify the impact on the concentrations of specific immunoglobulins (Ig) against SARS-CoV-2. A prospective, observational, exploratory pilot study in lactating women (n=12) who received the complete course of mRNA-based vaccines (BioNTech/Pfizer) against SARS-CoV-2 infection was conducted. Levels of antibodies directed to structural receptor-binding domain of the SARS-CoV-2 spike protein were determined by ELISA before and after pasteurization. IgA and IgG anti-SARS-CoV-2 immunoglobulin concentrations were significantly reduced after Holder pasteurization. Interestingly, there was a negative correlation between the initial amount of anti-SARS-CoV-2 antibodies and the percentage of their recovery after the pasteurization for both isotypes. Despite the partial loss of immunoglobulins still a high percentage of antibodies remained after the pasteurization, a mean of 70.53 (3.4)% of anti-SARS-CoV-2 IgA and 81.99 (21.89)% of IgG antibodies. Our study underscores the potential relevance of breast feeding or alternatively DHM to provide babies at risk with virus-specific SARS-CoV-2 antibodies thus protecting them against COVID-19.We thank the support and guidance of M.C. Collado and C. Martinez-Costa. We thank all the families who were involved in the study during this difficult time and in the middle of the COVID-19 pandemic as well as the collaborators of the MilkCORONA study team and, the support received by the research grant from LaMarató-TV3 (ref. 31/109/202106).N

NEGATIVE - XNAT - 3

Third part of Negative XNAT Images, in DICOM format

POSITIVE - XNAT - 2

Second part of Positive XNAT Images, in DICOM format

NEGATIVE - NPY Processed

Preprocessed negative images stored in numpy array format (npy