Атеросклероз при воспалительных заболеваниях суставов

АТЕРОСКЛЕРОЗСУСТАВОВ БОЛЕЗН

Методические указания к выполнению курсовых работ по фармакогнозии

УЧЕБНО-МЕТОДИЧЕСКИЕ ПОСОБИЯФАРМАКОГНОЗИ

Micro-Structuring of New Materials Combined with Electronic Polymers for Interfaces with Cells

Materials based on novel Off-Stoichiometry Thiol-Ene polymers, abbreviated OSTE, show promising properties as materials forlow cost and scalable manufacturing of micro- and nanosystems such as lab-on-chip devices. The OSTE materials have tunablemechanical properties, offer possibility for low temperature bonding to many surfaces via tunable surface chemistry, and can beused in soft lithography. Unlike the commonly used elastomer poly(dimethylsiloxane), PDMS, the OSTE materials have lowpermeability for gasses, are resistant to common solvents and can be more permanently surface modified.In this master’s thesis project, the OSTE materials have been evaluated with focus on compatibility with cells, possibility fornanostructuring using soft lithography and the use of OSTE as a flexible support for conducting polymers.Results from cell seeding studies with HEP G2 cells suggest that cells can proliferate on a low thiol off-stoichiometry OSTEmaterial for at least five days. The biocompatibility for this type of OSTE material may be similar to poly(styrene). However, highlevels of free thiol monomers in the material decrease cell viability considerably.By using soft lithography techniques it is possible to fabricate OSTE nanochannels with at least the dimensions of 400 nm x 15nm. Combined with the advantages of using the OSTE materials, such as low temperature bonding and possibility for stablesurface modifications, a candidate construction material for future development of systems for DNA analysis is at hand.OSTE can serve as a flexible support for an adsorbed film of a conducting polymer with the possibility for future applicationssuch as electronic interfaces in microsystems. In this project, a film of PEDOT:PSS with the electrical resistance of ~5 kΩ wascreated by adsorption to an flexible OSTE material. Furthermore, results suggest that it is possible to further optimize theconductivity and water resistance of PEDOT:PSS films on OSTE

Thiol-ene and Thiol-ene-epoxy Based Polymers for Biomedical Microdevices

Within healthcare there is a market pull for biomedical devices that can rapidly perform laboratory processes, such as diagnostic testing, in a hand-held format. For this reason, biomedical devices must become smaller, more sophisticated, and easier to use for a reasonable cost. However, despite the accelerating academic research on biomedical microdevices, and especially plastic-based microfluidic chips, there is still a gap between the inventions in academia and their benefit to society. To bridge this gap there is a need for new materials which both exhibit similar properties as industrial thermoplastics, and that enable rapid prototyping in academia. In this thesis, thiol-ene and thiol-ene-epoxy thermosets are evaluated both in terms of their suitability for rapid prototyping of biomedical microdevices and their potential for industrial manufacturing of “lab-on-chips”. The first part of the thesis focuses on material development of thiol-ene and thiol-ene-epoxy thermosets. Chemical and mechanical properties are studied, as well as in vitro biocompatibility with cells. The second part of the thesis focuses on microfabrication methods for both thermosets. This includes reaction injection molding, photostructuring, and surface modification. It is demonstrated how thiol-ene and thiol-ene-epoxy both provide advantageous thermo-mechanical properties and versatile surface modifications via “thiol-click chemistry”. In the end of the thesis, two applications for both polymer platforms are demonstrated. Firstly, thiol-ene is used for constructing nanoliter well arrays for liquid storage and on-demand electrochemical release. Secondly, thiol-ene-epoxy is used to enhance the biocompatibility of neural probes by tuning their flexibility. It is concluded that both thiol-ene and thiol-ene-epoxy thermosets exhibit several properties that are highly suitable for rapid prototyping as well as for scalable manufacturing of biomedical microdevices.QC 20171003</p

Polymer Nanoliter Well Arrays for Liquid Storage and Rapid On-demand Electrochemical Release

Polymer microfluidic systems are of increasing importance in several applications in biomedicine and biosensing. The integrated encapsulation, storage, and controlled release of small amounts of liquid in such systems remains an unresolved technical challenge. Here, we report two methods for the room-temperature and adhesive-free sealing of 1–330 nanoliter volumes of liquid in off-stoichiometry thiol-ene polymer well arrays by spontaneous bonding to 200 nm thin gold films. Sealed well arrays were stored for more than one month in a liquid environment with &lt;10% liquid loss, and for more than one week in air with minimal loss. We demonstrated that controlling the electrical potential and polarity over encapsulated wells allowed for selecting one of two well opening mechanisms: slow anodic electrochemical etching, or rapid electrolytic gas pressure-induced bursting of the gold film. The results may find potential applications in diagnostic testing, in vivo drug delivery, or in spatio-temporal release of chemical compounds in biological assays.QC 20180515</p

LONG-TERM STORAGE OF NANOLITRE AND PICOLITRE LIQUID VOLUMES IN POLYMER MICROFLUIDIC DEVICES

We introduce uncomplicated nanolitre (23 nL) and picolitre (3.5 pL) liquid volume encapsulation in Off-Stoichiometry Thiol-Ene-Epoxy polymer (OSTEmerTM322) wells using spontaneous room- temperature bonding of gold films to thiol and thioether groups present on the surface of the polymer for leak free sealing. First, we show liquid encapsulation within nL, and pL polymer wells by utilizing 100 nm thin Au-film transfer-bonding onto intermediately cured, and micropatterned OSTEmerTM322. This approach yielded 3 magnitude orders smaller liquid volume encapsulation than previously reported. Secondly, we show that encapsulated liquid can be stored for &gt;116 h. Finally, we demonstrate encapsulated liquid release by thermopneumatic bursting. We conclude that OSTEmerTM322 is excellent for metal-film sealant integration in polymer microfluidic devices. QC 20160404</p

Учебная программа по учебной дисциплине "Экономика предприятия"

Учебная программа "Экономика предприятия" кафедры "Экономика" для дневной формы получения образования: общее количество часов - 240, трудоемкость учебной дисциплины - 3 з.е., форма контроля знаний - экзамен, курсовая работа

Reaction injection molding and direct covalent bonding of OSTE+ polymer microfluidic devices

In this article, we present OSTE+RIM, a novel reaction injection molding (RIM) process that combines the merits of off-stoichiometric thiol–ene epoxy (OSTE+) thermosetting polymers with the fabrication of high quality microstructured parts. The process relies on the dual polymerization reactions of OSTE+ polymers, where the first curing step is used in OSTE+RIM for molding intermediately polymerized parts with well-defined shapes and reactive surface chemistries. In the facile back-end processing, the replicated parts are directly and covalently bonded and become fully polymerized using the second curing step, generating complete microfluidic devices. To achieve unprecedented rapid processing, high replication fidelity and low residual stress, OSTE+RIM uniquely incorporates temperature stabilization and shrinkage compensation of the OSTE+ polymerization during molding. Two different OSTE+ formulations were characterized and used for the OSTE+RIM fabrication of optically transparent, warp-free and natively hydrophilic microscopy glass slide format microfluidic demonstrator devices, featuring a storage modulus of 2.3 GPa and tolerating pressures of at least 4 bars. QC 201500618</p

E-Beam Nanostructuring and Direct Click Biofunctionalization of Thiol–Ene Resist

Electron beam lithography (EBL) is of major importance for ultraminiaturized biohybrid system fabrication, as it allows combining biomolecular patterning and mechanical structure definition on the nanoscale. Existing methods are limited by multistep biomolecule immobilization procedures, harsh processing conditions that are harmful to sensitive biomolecules, or the structural properties of the resulting protein monolayers or hydrogel-based resists. This work introduces a thiol-ene EBL resist with chemically reactive thiol groups on its native surface that allow the direct and selective "click" immobilization of biomolecules under benign processing conditions. We constructed EBL structured features of size down to 20 nm, and direct functionalized the nanostructures with a sandwich of biotin and streptavidin. The facile combination of polymer nanostructuring with biomolecule immobilization enables mechanically robust biohybrid components of interest for nanoscale biomedical, electronic, photonic, and robotic applications.QC 20181114</p

Characterisation of the KTP channel TALK-1 in the endocrine pancreas

Ion channels are instrumental in the regulated control of insulin secretion from the pancreatic islets. We therefore investigated the role of a novel potassium (K+) channel, TALK-1, In the endocrine pancreas. TALK-1 is a member of the twin-pore potassium (K2P) channel family, which are characterised by their unique dual pore domains and background conductance activity that Is active across the physiological range of membrane potentials. Four splice variants of human TALK-1 have previously been identified. We found that only one variant; the equivalent of human TALK-1 b is expressed in the rodent. RT-PCR and quantitative RT-PCR data revealed that TALK-1 has a limited expression profile and is most highly expressed in the islets of Langerhans in rodent and human tissues.EThOS - Electronic Theses Online ServiceGBUnited Kingdo